Fast True Confocal Scanning reduces photobleaching and increases the fluorescence yield at identical acquisition times. The long-lasting triplet state (or any other “dark state”) is less populated when the illumination is applied in shorter pulses at the same intensity. Consequently, more fluorochromes are available for the fluorescence process (brighter images) and fewer fluorochromes disintegrate from triplet states or excited triplet states (less bleaching).
The obvious has been explored. These days, biologists strive to identify and analyze hidden and rare events. The task is tackled by automatically screening large numbers of objects – typically growing in multi-well plates – over a long period of time. When an interesting feature is identified (manually or by means of computed recognition), modern systems can automatically monitor and record these events at high resolution.
STED uses a differential method of two different diffraction patterns, where one pattern excites and the second pattern de-excites fluorochromes. The residual excited area is controllable by intensity down to (theoretically) zero – unlimited resolution.
Hoffman modulation contrast has established itself as a standard for the observation of unstained, low-contrast biological specimens. The integration of the modulator in the beam path of themodern inverted microscopes allows a wide range of brightfield or phase objectives to be used, rather than a small selection of special objectives.
“Confocal Microscopy” refers to a particular optical microscope that allows recording optical sections. Optical sectioning is achieved in a confocal system by illuminating and observing a single diffraction limited spot.
An insight into the unknown world of the deep sea was given in an exhibition staged by the Senckenberg Society together with the Natural History Museum in Basel, Switzerland. Shown in Frankfurt am Main and Berlin, Germany, the "Deep Sea" exhibition was a huge public success in 2009. From May to September 2010, the exhibition has been shown in the Natural History Museum in London. Scientists of the Senckenberg Society work with stereomicroscopes and digital cameras both in the laboratory and on the research ships.
Laser dissection is used in a large number of research fields, e.g. neurology, cancer research, plant analysis. Here, user report on the research results they have attained by using laser microdissection.
Brain research using Confocal and Multiphoton Microscopy.
Using imaging techniques such as confocal and two-photon microscopy, neuronal dendritic arborization of neurons and their synaptic interconnections can be visualized.