Contact & Support
Header Image
  • Clearing Procedures for Deep Tissue Imaging

    Multi-channel multiphoton microscopy with dedicated optics for CLARITY. Why clearing? Curiosity is human nature. And nothing attracts as much curiosity as the inside of living organisms. While in ancient times those who cut human bodies open to do research were put to death, and modern anatomy started only after Pope Clement VII allowed dissection, we can now watch brains working in living animals – and have a good chance of soon being able to interfere with the observed activities for healing (or control) purposes.
    Read article
  • Video Interview with Timo Zimmermann

    The first super-resolution image he saw was an eye opener for him: "It was not just structures that got smaller. I was looking at a sample that I specifically had high hopes of seeing another layer of complexity and this actually was there."
    Read article
  • Brief Introduction to Ultramicrotomy

    The usual thicknesses for transmission electron microscopic examinations range between 20 nm and 150 nm. Ultramicrotomy is a fast and clean method of producing ultra-thin sections of biological samples as well as polymers, rubber, ductile and even hard and brittle materials. A key advantage of ultramicrotomy is the size and homogeneity of the electron-transparent area of specimens prepared with this technique.
    Read article
  • Are Your Digital Microscope Measurements Accurate and Reliable?

    For certain applications, such as the production and maintenance of automobiles, aircraft, or power plants, quality control and reliability assurance, as well as safety and health inspection, accurate and reliable image data with precise calibration are very important. Digital microscopes are appealing for a wide range of technical applications in various industries, such as automotive, aerospace, precision engineering, microelectronics, and medical devices.
    Read article
  • Video Interviews with Kees Jalink

    Kees Jalink's group at the Netherlands Cancer Institute in Amsterdam, The Netherlands, explores signal transduction pathways and cell adhesion processes in cancer cells. In his eyes especially the new three-dimensional nanoscopic view of the relevant structure of interest is an essential feature to get the full picture.
    Read article
  • 125 Years of Comparison Microscopy – From the "camera lucida" to the Automated Comparison Bridge

    To be able to optically compare two objects with scientific accuracy, it must be possible to view them at the same time. This is particularly true for comparing small objects that can only be visualized with the aid of an optical magnification system. If you only have one microscope and have to look at the objects alternately, you need an excellent memory and can never rule out the risk of judgment errors, especially when examining objects with only minor differences in structure, color or profile.
    Read article
  • The Environment Makes the Stem Cell

    A recent publication in Nature shows that all stem cells divide and compete for niche space by passively "kicking out" others so that eventually one stem cell takes over the whole niche. Jacco van Rheenen and Saskia Ellenbroek talk about a new method of intravital imaging, which allows following the fate of individual stem cells over time in vivo and explains the new paradigm for stem cell development in the intestinal stem cell niche.
    Read article
  • Smart Control for Resonant Galvo Scanners

    High time-resolution confocal microscopy (HTRCLSM) requires fast scanning devices. Whereas non-resonant galvo scanners allow full position control, but only at slow speed, resonant scanners allow ~25,000 lines per second, but offer much less positioning freedom. To still allow zoom and pan functions, several approaches have been tried, with varying success. The Leica confocal microscopes od the TCS series use a very smart solution that enables stepless zooming with short switching times.
    Read article
  • The Basic Principles of Laser Microdissection

    In contrast to other systems which use a fixed laserfocus for dissection, Leica Microsystems' LMD systems guide the laserfocus for dissection. This unique feature allows highly precise laser dissection independent of the stage accuracy. This tutorial describes the basic principles of the laser guidance and how the laser can be adjusted for perfect cutting results.
    Read article
  • STED Publication List

    It is already 20 years ago that STED microscopy was first described by Stephan Hell. In the meantime it has become an important tool to study subcellular structures beyond the diffraction limit. This reference list gives an overview about the technological development of STED nanoscopy and also shows selected publications performed on a Leica STED instrument.
    Read article
  • Analysis of Oil Shale as an Alternative Source of Energy

    For some years now, the search for alternative sources of raw materials has concentrated on oil shale deposits. As the exploitation of these raw materials is still extremely complex and expensive, analysis of oil shale samples is therefore extremely important for identifying the deposit’s potential in advance and optimizing mining methods.
    Read article
  • Triple Ion Beam Cutting of Diamond/Al Composites for Interface Characterization

    The microstructure of new materials with heterogenic components is extremely difficult to study. As mechanical polishing often not leads to smooth surfaces, a novel ion beam cutting technique has proven to be very effective. In this article, the applicability of a novel triple ion beam (TIB) cutting technique for a clean metallographic preparation of the diamond/Al composites will be described.
    Read article
  • Video Interview with Stephan Sigrist

    Stephan Sigrist is professor for biology at the Freie Universität Berlin in Germany. His research focus are synapses, synaptic information transfer and processing between neurons in the developing drosophila larva. His aim is to understand how synapses actually get diversified in our brains.
    Read article
  • Webinar: Increase Efficiency and Productivity at your Microscope Workstation

    Ergonomic workstations improve more than comfort – they improve the overall wellbeing of the work force. With a few simple adjustments and the right equipment to provide better posture, microscope users can dramatically increase productivity, focus, and efficiency on the job, while reducing health issues in both the short and long term.
    Read article
  • Video Interview with Dr. Yasushi Okada

    Yasushi Okada, team leader at Riken Quantitative Biology Center in Osaka, Japan, investigates vesicular transport mechanisms in neuronal cells. As the size of transported vesicles is below 100 nm and the diameter of microtubules is about 25 nm, he uses super-resolution techniques to study the sophisticated machinery of neuronal transport.
    Read article
  • Webinar: Innovative Sample Preparation Method of Heterogeneous Materials for EM Observation

    Sample Preparation is a prerequisite for microscopy. It’s essential for achieving accurate and reproducible results. To get the best results in interfacial characterization, Leica Microsystems developed a triple ion beam cutting technique for electron microscopy observation. This technique is integrated into the ion beam slope cutter Leica EM TIC 3X and optimizes the processing conditions of sample preparation to reveal the nano-sized interfacial features of the specimen.
    Read article
  • Hunting Down the Hay Bacillus – Educational Microscopes in Biology Teaching

    Learning begins with perception. Sensory impressions are branded in our mind and become the building blocks of knowledge. The more intensively young people are involved in the lesson and the more experiences they can make themselves, the easier they find it to learn. Hands-on microscopy is therefore a key component of modern science teaching, for example at the Philippinum Grammar School in Weilburg, where students love working with Leica educational microscopes.
    Read article
  • Huygens STED Deconvolution Quick Guide

    This document is intended to give Leica STED users a brief introduction to deconvolving images on Huygens Professional using images acquired with the Leica TCS SP8 STED 3X microscope. For a more detailed description of Huygens Professional, including additional features and tools, please visit the Manuals section of SVI (www.svi.nl).
    Read article
  • Bird Park Gives Fascinating Insights into the Variety of Nature

    The Vogel- und Naturschutz-Tierpark Herborn to the north of Frankfurt may be small, but it’s always a great experience for school classes – and not only because it’s home to more than 300 animals of 80 different species, from South African blue cranes and white storks to meerkats and muntjac deer. Another special highlight for schoolchildren is the opportunity to look through a stereomicroscope and gain fascinating insights into nature.
    Read article
  • New Paths in Glaucoma Therapy – Operation Video Supports Knowledge Transfer in Eye Surgery

    If medication and laser techniques have no prospect of success in the treatment of glaucoma, surgery is essential to stop further damage to the optic nerve. A new surgical method is showing promising results for reducing the potential complications of an operation. Professor Dr. Dr. h.c. Franz Grehn, Director of Würzburg University Eye Hospital, has developed the new method and produced a video to accompany a clinical comparison study. This film was awarded the Video Award 2013 sponsored by Leica Microsystems at the 111th Congress of the German Ophthalmological Society.
    Read article
  • Tracking Glomerular Fate Over Long Time Distances

    Multi-channel multiphoton microscopy with dedicated optics for CLARITY. The glomerular filtration barrier (GFB) is a complex spatial structure within the kidney glomerulis where ultrafiltration takes place. Podocytes are critical elements of the GFB and take part in the filtration process. They have been shown to be involved in the development of kidney diseases. However, due to technical limitations, the mechanism of glomerular pathology is not well understood.
    Read article
  • Fully Automated Tracking of Chloroplasts in Elodea Leaf Cells from 4D Image Data

    Fully automated tracking of moving 3D structures in living cells represents several challenges that are typical to the analysis of biological image data. Taking 4D image data from chloroplasts in living Elodea leaf cells as an example we demonstrate new approaches to meet these challenges. Chloroplasts are surprisingly dynamic organelles: They move extensively throughout cells, they regularly split or fuse and their shape changes constantly.
    Read article
  • Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

    The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as 20 nanometers. GSDIM is suitable for a wide range of samples.
    Read article
  • Map the Brain with CLARITY

    Imaging whole brains with CLARITY and multiphoton microscopy. Image a whole brain without sectioning? Investigate neuronal circuits without reconstruction? Perform molecular phenotyping without destroying subcellular structures? Understanding the brain with molecular resolution and global scope has always been challenging. The novel CLARITY method, developed by the Deisseroth laboratory at Stanford University, USA, pushes the barrier of deep tissue imaging a big step ahead.
    Read article