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Laser Microdissection Publication List

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

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Topics & Tags

DNA Research

Loss of wild-type p53 promotes mutant p53-driven metastasis through acquisition of survival and tumor-initiating properties
Nakayama M, Pyo Hong C, Oshima H, Sakai E, Kim S-J, Oshima M
Nat Commun. 2020 May 11;11(1):2333. doi: 10.1038/s41467-020-16245-1.

Molecular identifcation of Nectriaceae in infections of apple replant disease afected roots collected by Harris Uni‑Core punching or laser microdissection
Popp C, Wamhoff D, Winkelmann T, Maiss E, Grunewaldt-Stöcker G
Journal of Plant Diseases and Protection, 9 May 2020,  https://doi.org/10.1007/s41348-020-00333-x

Mapping the spreading routes of lymphatic metastases in human colorectal cancer
Zhang C, Zhang L, Xu T, Xue R, Yu L, Zhu Y, Wu Y, Zhang Q, Li D, Shen S, Tan D, Bai F, Zhang H
Nat Commun. 2020 Apr 24;11(1):1993. doi: 10.1038/s41467-020-15886-6.

RNA Research

Timekeeping in the hindbrain: a multi-oscillatory circadian centre in the mouse dorsal vagal complex
Chrobok L, Northeast RC, Myung J, Cunningham PS, Petit C, Piggins HD
Commun Biol. 2020 May 8;3(1):225. doi: 10.1038/s42003-020-0960-y.

Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis
Balestrini R, Fiorilli V
Methods Mol Biol. 2020;2146:171-184. doi: 10.1007/978-1-0716-0603-2_13.

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
Comba A, Dunn PJ, Kish PE, Kadiyala P, Kahana A, Castro MG, Lowenstein PR
J Vis Exp. 2020 Apr 12;(158). doi: 10.3791/60939.

Proteomics, Lipidomics, Metabolomics

Glutathione peroxidase-1 overexpression reduces oxidative stress, and improves pathology and proteome remodeling in the kidneys of old mice
Chu Y, Lan RS, Huang R, Feng H, Kumar R, Dayal S, Chan K-S, Dai D-F
Aging Cell. 2020 May 13. doi: 10.1111/acel.13154.

STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer
Oberhuber M, Pecoraro M, Rusz M, Oberhuber G, Wieselberg M, Haslinger P, Gurnhofer E, Schlederer M, Limberger T, Lagger S, Pencik J, Kodajova P, Högler S, Stockmaier G, Grund-Gröschke S, Aberger F, Bolis M, Theurillat JP, Wiebringhaus R, Weiss T, Haitel A, Brehme M, Wadsak W, Griss J, Mohr T, Hofer A, Jäger A, Pollheimer J, Egger G, Koellensperger G, Mann M, Hantusch B, Kenner L
Mol Syst Biol. 2020 Apr;16(4):e9247. doi: 10.15252/msb.20199247.

Molecular Analysis of Clinically Defined Subsets of High-Grade Serous Ovarian Cancer
Lee S, Zhao L, Rojas C, Bateman NW, Yao H, Lara OD, Celestino J, Morgan MB, Nguyen TV, Conrads KA, Rangel KM, Dood RL, Hajek RA, Fawcett GL, Chu RA, Wilson K, Loffredo JL, Viollet C, Jazaeri AA, Dalgard CL, Mao X, Song X, Zhou M, Hood BL, Banskota N, Wilkerson MD, Te J, Soltis AR, Roman K, Dunn A, Cordover D, Eterovic AK, Liu J, Burks JK, Baggerly KA, Fleming ND, Lu KH, Westin SN, Coleman RL, Mills GB, Casablanca Y, Zhang J, Conrads TP, Maxwell GL, Futreal PA, Sood AK
Cell Rep. 2020 Apr 14;31(2):107502. doi: 10.1016/j.celrep.2020.03.066.

Fluorescence Applications

Combined maternal exposure to cypermethrin and stress affect embryonic brain and placental outcomes in mice
Elser BA, Kayali K, Dhakal R, O'Hare B, Wang K, Lehmler HJ, Stevens HE
Toxicol Sci. 2020 Mar 19. pii: kfaa040. doi: 10.1093/toxsci/kfaa040.

Dynamically Monitoring the Clonal Evolution of Lung Cancer Based on the Molecular Characterization of Circulating Tumor Cells using Aptamer Cocktail-Modified Nanosubstrates
Zhu Y, Zou C, Zhang J, Jiang W, Guan F, Tang K, Li S, Li G, Wang J, Ke Z
ACS Appl Mater Interfaces. 2020 Jan 15. doi: 10.1021/acsami.9b22234.

Cav2.3 channels contribute to dopaminergic neuron loss in a model of Parkinson’s disease
Benkert J, Hess S, Roy S, Beccano-Kelly D, Wiederspohn N, Duda J, Simons C, Patil K, Gaifullina A,  Mannal N, Dragicevic E, Spaich D, Müller S, Nemeth J, Hollmann H, Deuter N, Mousba Y, Kubisch C,
Poetschke C, Striessnig J, Pongs O, Schneider T, Wade-Martins R, Patel S, Parlato R, Frank T, Kloppenburg P & Liss B
Nat Commun 10, 5094 (8 Nov 2019) doi:10.1038/s41467-019-12834-x

Cancer Research

Loss of wild-type p53 promotes mutant p53-driven metastasis through acquisition of survival and tumor-initiating properties
Nakayama M, Pyo Hong C, Oshima H, Sakai E, Kim S-J, Oshima M
Nat Commun. 2020 May 11;11(1):2333. doi: 10.1038/s41467-020-16245-1.

Integrative Transcriptome Analyses of the Human Fallopian Tube: Fimbria and Ampulla-Site of Origin of Serous Carcinoma of the Ovary
Sowamber R, Nelson O, Dodds L, DeCastro V, Paudel I, Milea A, Considine M, Cope L, Pinto A, Schlumbrecht M, Slomovitz B, Shaw PA, George SHL
Cancers (Basel). 2020 Apr 27;12(5). pii: E1090. doi: 10.3390/cancers12051090.

Mapping the spreading routes of lymphatic metastases in human colorectal cancer
Zhang C, Zhang L, Xu T, Xue R, Yu L, Zhu Y, Wu Y, Zhang Q, Li D, Shen S, Tan D, Bai F, Zhang H
Nat Commun. 2020 Apr 24;11(1):1993. doi: 10.1038/s41467-020-15886-6.

Neurosciences

Timekeeping in the hindbrain: a multi-oscillatory circadian centre in the mouse dorsal vagal complex
Chrobok L, Northeast RC, Myung J, Cunningham PS, Petit C, Piggins HD
Commun Biol. 2020 May 8;3(1):225. doi: 10.1038/s42003-020-0960-y.

Learning-induced mRNA alterations in olfactory bulb mitral cells in neonatal rats
Nartey MN, Peña-Castillo L, LeGrow M, Doré J, Bhattacharya S, Darby-King A, Carew SJ, Yuan Q, Harley CW, McLean JH
Learn Mem. 2020 Apr 15;27(5):209-221. doi: 10.1101/lm.051177.119.

Semaglutide lowers body weight in rodents via distributed neural pathways
Gabery S, Salinas CG, Paulsen SJ, Ahnfelt-Rønne J, Alanentalo T, Baquero AF, Buckley ST, Farkas E, Fekete C, Frederiksen KS, Helms HCC, Jeppesen JF, John LM, Pyke C, Nøhr J, Lu TT, Polex-Wolf J, Prevot V, Raun K, Simonsen L, Sun G, Szilvásy-Szabó A, Willenbrock H, Secher A, Knudsen LB
JCI Insight. 2020 Mar 26;5(6). pii: 133429. doi: 10.1172/jci.insight.133429.

Clinical Applications

p57-discordant villi in hydropic products of conception: a clinicopathological study of 70 cases
Gaillot-Durand L, Patrier S, Aziza J, Devisme L, Riera A-C, Marcorelles P, Pelluard F, Gasser B, Mauduit C, Hajri T, Massardier J, Bolze P-A, Golfier F, Devouassoux-Shisheboran M, Allias F
Human Pathology, 6 May 2020, ISSN 0046-8177

Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu
Zhang D, Kinloch AJ, Srinath A, Shenkar R, Girard R, Lightle R, Moore T, Koskimäki J, Mohsin A, Carrión-Penagos J, Romanos S, Shen L, Clark MR, Shi C, Awad IA
J Autoimmun. 2020 Apr 30:102469. doi: 10.1016/j.jaut.2020.102469.

Constitutive hydrogen inhalation prevents vascular remodeling via reduction of oxidative stress
Kiyoi T, Liu S, Takemasa E, Nakaoka H, Hato N, Mogi M
PLoS One. 2020 Apr 17;15(4):e0227582. doi: 10.1371/journal.pone.0227582. eCollection 2020.

Developmental Research, Epigenetics & Aging

Glutathione peroxidase-1 overexpression reduces oxidative stress, and improves pathology and proteome remodeling in the kidneys of old mice
Chu Y, Lan RS, Huang R, Feng H, Kumar R, Dayal S, Chan K-S, Dai D-F
Aging Cell. 2020 May 13. doi: 10.1111/acel.13154.

Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos
Zhou X, Liu Z, Shen K, Zhao P, Sun MX
Nat Commun. 2020 Mar 13;11(1):1366. doi: 10.1038/s41467-020-15189-w

Tsukushi is essential for the development of the inner ear
Miwa T, Ohta K, Ito N, Hattori S, Miyakawa T, Takeo T, Nakagata N, Song WJ, Minoda R
Mol Brain. 2020 Mar 3;13(1):29. doi: 10.1186/s13041-020-00570-z.

Plant Research

Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis
Balestrini R, Fiorilli V
Methods Mol Biol. 2020;2146:171-184. doi: 10.1007/978-1-0716-0603-2_13.

Molecular identifcation of Nectriaceae in infections of apple replant disease afected roots collected by Harris Uni‑Core punching or laser microdissection
Popp C, Wamhoff D, Winkelmann T, Maiss E, Grunewaldt-Stöcker G
Journal of Plant Diseases and Protection, 9 May 2020,  doi.org/10.1007/s41348-020-00333-x

Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos
Zhou X, Liu Z, Shen K, Zhao P, Sun MX
Nat Commun. 2020 Mar 13;11(1):1366. doi: 10.1038/s41467-020-15189-w

Forensics

Compatibility of the ForenSeq™ DNA Signature Prep Kit with laser microdissected cells: An exploration of issues that arise with samples containing low cell numbers
England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison SA
Forensic Science International: Genetics, 1 May 2020, 102278

Application of Optical Trapping to Obtain Single-Source STR Profiles fr ofiles from Forensically Rele ensically Relevant Body Fluid Mixtur ant Body Fluid Mixtures with es with Modified DNA Analysis Workflow
O`Brien BJ
Thesis, 28 April 2020

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Information for practitioners

 

Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Ezrin T567 phosphorylation regulates migration and invasion of ectopic endometrial stromal cells by changing actin cytoskeleton
Chen C, Ye C, Xia J, Zhou Y, Wu R
Life Sci. 2020 May 4:117681. doi: 10.1016/j.lfs.2020.117681.

Cell-type diversity and regionalized gene expression in the planarian intestine
Forsthoefel DJ, Cejda NI, Khan UW, Newmark PA
Elife. 2020 Apr 2;9. pii: e52613. doi: 10.7554/eLife.52613.

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye
Okano K, Saratani Y, Tamasawa A, Shoji Y, Toda R, Okano T
Sci Rep. 2020 Mar 19;10(1):5056. doi: 10.1038/s41598-020-61877-4.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.