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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Table of Content

DNA Research

A germline MBD4 mutation was identified in a patient with colorectal oligopolyposis and early‑onset cancer: A case report
Tanakaya K, Kumamoto K, Tada Y, Eguchi H, Ishibashi K, Idani H, Tachikawa T, Akagi K, Okazaki Y, Ishida H
Oncol Rep. 2019 Jul 17. doi: 10.3892/or.2019.7239.

In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9
Steinecke A, Kurabayashi N, Hayano Y, Ishino Y, Taniguchi H
Cell Rep. 2019 Jul 9;28(2):325-331.e4. doi: 10.1016/j.celrep.2019.06.038.

Genomic landscape of epithelium with low-grade atypia on gastric cancer after Helicobacter pylori eradiation therapy
Masuda K, Urabe Y, Ito M, Ono A, Clair Nelson H, Nakamura K, Kotachi T, Boda T, Tanaka S, Chayama
J Gastroenterol. 2019 Jun 13. doi: 10.1007/s00535-019-01596-4.

RNA Research

Conditional Deletion of Eaf1 Induces Murine Prostatic Intraepithelial Neoplasia in Mice
Pascal LE, Su F, Wang D, Ai J, Song Q, Wang Y, O'Malley KJ, Cross B, Rigatti LH, Green A, Dhir R, Wang Z
Neoplasia. 2019 Aug;21(8):752-764. doi: 10.1016/j.neo.2019.05.005.#

Adenosine stimulates neuromedin U mRNA expression in the rat pars tuberalis
Aizawa S, Gu T, Kaminoda A, Fujioka R, Ojima F, Sakata I, Sakai T, Ogoshi M, Takahashi S, Takeuchi S
Mol Cell Endocrinol. 2019 Jul 22;496:110518. doi: 10.1016/j.mce.2019.110518.

Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
Ma P, Song NN, Li Y, Zhang Q, Zhang L, Zhang L, Kong Q, Ma L, Yang X, Ren B, Li C, Zhao X, Li Y, Xu Y, Gao X, Ding YQ, Mao B
Cell Rep. 2019 Jul 9;28(2):541-553.e4. doi: 10.1016/j.celrep.2019.06.017.

Proteomics, Lipidomics, Metabolomics

MASS SPECTROMETRY-BASED PROTEOME PROFILE MAY BE USEFUL TO DIFFERENTIATE ADENOID CYSTIC CARCINOMA FROM POLYMORPHOUS ADENOCARCINOMA OF SALIVARY GLANDS,
Fonseca FP, Macedo CCS, dos Santos Costa FS, Leme AFP, Rodrigues RR, Pontes HAR, Altemani A, van Heerden WFP, Martins MD, de Almeida OP, Silva ARS, Lopes MA, Vargas PA
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2019 Aug 1, ISSN 2212-4403

Lewy pathology in Parkinson's disease consists of crowded organelles and lipid membranes
Shahmoradian SH, Lewis AJ, Genoud C, Hench J, Moors TE, Navarro PP, Castaño-Díez D, Schweighauser G, Graff-Meyer A, Goldie KN, Sütterlin R, Huisman E, Ingrassia A, Gier Y, Rozemuller AJM, Wang J, Paepe A, Erny J, Staempfli A, Hoernschemeyer J, Großerüschkamp F, Niedieker D, El-Mashtoly SF, Quadri M, Van IJcken WFJ, Bonifati V, Gerwert K, Bohrmann B, Frank S, Britschgi M, Stahlberg H, Van de Berg WDJ, Lauer ME
Nat Neurosci. 2019 Jul;22(7):1099-1109. doi: 10.1038/s41593-019-0423-2.

Precise co-registration of mass spectrometry imaging, histology, and laser microdissection-based omics
Dewez F, Martin-Lorenzo M, Herfs M, Baiwir D, Mazzucchelli G, De Pauw E, Heeren RMA, Balluff B
Anal Bioanal Chem. 2019 Jul 1. doi: 10.1007/s00216-019-01983-z.

Fluorescence Applications

In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9
Steinecke A, Kurabayashi N, Hayano Y, Ishino Y, Taniguchi H
Cell Rep. 2019 Jul 9;28(2):325-331.e4. doi: 10.1016/j.celrep.2019.06.038.

Self-seeding circulating tumor cells promote the proliferation and metastasis of human osteosarcoma by upregulating interleukin-8
Liu T, Ma Q, Zhang Y, Wang X, Xu K, Yan K, Dong W, Fan Q, Zhang Y, Qiu X
Cell Death Dis. 2019 Jul 31;10(8):575. doi: 10.1038/s41419-019-1795-7.

Tissue-Specific Metabolite Profiling on the Different Parts of Bolting and Unbolting Peucedanum praeruptorum Dunn (Qianhu) by Laser Microdissection Combined with UPLC-Q/TOF⁻MS and HPLC⁻DAD
Chen LL, Chu SS, Zhang L, Xie J, Dai M, Wu X, Peng HS
Molecules. 2019 Apr 11;24(7). pii: E1439. doi: 10.3390/molecules24071439.

Cancer Research

Conditional Deletion of Eaf1 Induces Murine Prostatic Intraepithelial Neoplasia in Mice
Pascal LE, Su F, Wang D, Ai J, Song Q, Wang Y, O'Malley KJ, Cross B, Rigatti LH, Green A, Dhir R, Wang Z
Neoplasia. 2019 Aug;21(8):752-764. doi: 10.1016/j.neo.2019.05.005.#

Self-seeding circulating tumor cells promote the proliferation and metastasis of human osteosarcoma by upregulating interleukin-8
Liu T, Ma Q, Zhang Y, Wang X, Xu K, Yan K, Dong W, Fan Q, Zhang Y, Qiu X
Cell Death Dis. 2019 Jul 31;10(8):575. doi: 10.1038/s41419-019-1795-7.

Precise co-registration of mass spectrometry imaging, histology, and laser microdissection-based omics
Dewez F, Martin-Lorenzo M, Herfs M, Baiwir D, Mazzucchelli G, De Pauw E, Heeren RMA, Balluff B
Anal Bioanal Chem. 2019 Jul 1. doi: 10.1007/s00216-019-01983-z.

Neurosciences

A Fully Integrated Spintip-Based Approach for Sensitive and Quantitative Profiling of Region-Resolved in Vivo Brain Glycoproteome
Huang P, Li H, Gao W, Cai Z, Tian R
Anal Chem. 2019 Jul 16;91(14):9181-9189. doi: 10.1021/acs.analchem.9b01930.

In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9
Steinecke A, Kurabayashi N, Hayano Y, Ishino Y, Taniguchi H
Cell Rep. 2019 Jul 9;28(2):325-331.e4. doi: 10.1016/j.celrep.2019.06.038.

Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
Ma P, Song NN, Li Y, Zhang Q, Zhang L, Zhang L, Kong Q, Ma L, Yang X, Ren B, Li C, Zhao X, Li Y, Xu Y, Gao X, Ding YQ, Mao B
Cell Rep. 2019 Jul 9;28(2):541-553.e4. doi: 10.1016/j.celrep.2019.06.017.

Clinical Applications

A germline MBD4 mutation was identified in a patient with colorectal oligopolyposis and early‑onset cancer: A case report
Tanakaya K, Kumamoto K, Tada Y, Eguchi H, Ishibashi K, Idani H, Tachikawa T, Akagi K, Okazaki Y, Ishida H
Oncol Rep. 2019 Jul 17. doi: 10.3892/or.2019.7239.

A pathological study of Leishmania infantum natural infection in European rabbits (Oryctolagus cuniculus) and Iberian hares (Lepus granatensis)
Ortega-García MV, Salguero FJ, Rodríguez-Bertos A, Moreno I, García N, García-Seco T, Luz Torre G, Domínguez L, Domínguez M
Transbound Emerg Dis. 2019 Jul 24. doi: 10.1111/tbed.13305.

Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation
Shimizu H, Sakimoto T, Yamagami S
Sci Rep. 2019 Jul 3;9(1):9596. doi: 10.1038/s41598-019-46116-9.

Developmental Research, Epigenetics & Aging

Uterine spiral artery muscle dedifferentiation
Robson A, Lash GE, Innes BA, Zhang JY, Robson SC, Bulmer JN
Hum Reprod. 2019 Jul 26. pii: dez124. doi: 10.1093/humrep/dez124.

Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
Ma P, Song NN, Li Y, Zhang Q, Zhang L, Zhang L, Kong Q, Ma L, Yang X, Ren B, Li C, Zhao X, Li Y, Xu Y, Gao X, Ding YQ, Mao B
Cell Rep. 2019 Jul 9;28(2):541-553.e4. doi: 10.1016/j.celrep.2019.06.017.

Maternal High Fat Diet and in-Utero Metformin Exposure Significantly Impact upon the Fetal Renal Proteome of Male Mice
Nüsken E, Turnwald EM, Fink G, Voggel J, Yosy C, Kretschmer T, Handwerk M, Wohlfarth M, Weber LT, Hucklenbruch-Rother E, Dötsch J, Nüsken KD, Appel S
J Clin Med. 2019 May 11;8(5). pii: E663. doi: 10.3390/jcm8050663.

Plant Research

Tissue-Specific Metabolite Profiling on the Different Parts of Bolting and Unbolting Peucedanum praeruptorum Dunn (Qianhu) by Laser Microdissection Combined with UPLC-Q/TOF⁻MS and HPLC⁻DAD
Chen LL, Chu SS, Zhang L, Xie J, Dai M, Wu X, Peng HS
Molecules. 2019 Apr 11;24(7). pii: E1439. doi: 10.3390/molecules24071439.

Transcriptome landscape of the early Brassica napus seed
Ziegler DJ, Khan D, Kalichuk JL, Becker MG, Belmonte MF
J Integr Plant Biol. 2019 Apr 2. doi: 10.1111/jipb.12812.

Transcriptome analysis of Rlm2-mediated host immunity in the Brassica napus-Leptosphaeria maculans pathosystem
Becker MG, Haddadi P, Wan J, Adam L, Walker P, Larkan N, Daayf F, Borhan MH, Belmonte MF
Mol Plant Microbe Interact. 2019 Apr 2. doi: 10.1094/MPMI-01-19-0028-R.

Forensics

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Precise co-registration of mass spectrometry imaging, histology, and laser microdissection-based omics
Dewez F, Martin-Lorenzo M, Herfs M, Baiwir D, Mazzucchelli G, De Pauw E, Heeren RMA, Balluff B
Anal Bioanal Chem. 2019 Jul 1. doi: 10.1007/s00216-019-01983-z.

The roles of Centrin 2 and Dynein Light Chain 8a in apical secretory organelles discharge of Toxoplasma gondii
Lentini G, Dubois DJ, Maco B, Soldati-Favre D, Frénal K
Traffic. 2019 Jun 17. doi: 10.1111/tra.12673.

Standardizing Proteomics Workflow for Liquid Chromatography-Mass Spectrometry: Technical and Statistical Considerations
Srivastava S, Merchant M, Rai A, Rai SN
J Proteomics Bioinform 12: 048-055. doi: 10.4172/0974-276X.1000496

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.