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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Table of Content

DNA Research

Long-term Risk of Malignancy in Branch Duct Intraductal Papillary Mucinous Neoplasms
Oyama H, Tada M, Takagi K, Tateishi K, Hamada T, Nakai Y, Hakuta R, Ijichi H, Ishigaki K, Kanai S, Kogure H, Mizuno S, Saito K, Saito T, Sato T, Suzuki T, Takahara N, Morishita Y, Arita J, Hasegawa K, Tanaka M, Fukayama M, Koike K
Gastroenterology. 2019 Aug 29. pii: S0016-5085(19)41257-2. doi: 10.1053/j.gastro.2019.08.032.

A germline MBD4 mutation was identified in a patient with colorectal oligopolyposis and early‑onset cancer: A case report
Tanakaya K, Kumamoto K, Tada Y, Eguchi H, Ishibashi K, Idani H, Tachikawa T, Akagi K, Okazaki Y, Ishida H
Oncol Rep. 2019 Jul 17. doi: 10.3892/or.2019.7239.

In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9
Steinecke A, Kurabayashi N, Hayano Y, Ishino Y, Taniguchi H
Cell Rep. 2019 Jul 9;28(2):325-331.e4. doi: 10.1016/j.celrep.2019.06.038.

RNA Research

The median eminence as the hypothalamic area involved in rapid transfer of glucose to the brain: functional and cellular mechanisms
Martínez F, Cifuentes M, Tapia JC, Nualart F
J Mol Med (Berl). 2019 Aug;97(8):1085-1097. doi: 10.1007/s00109-019-01799-5.

Ischemic axonal injury up-regulates MARK4 in cortical neurons and primes tau phosphorylation and aggregation
Hayden EY, Putman J, Nunez S, Shin WS, Oberoi M, Charreton M, Dutta S, Li Z, Komuro Y, Joy MT, Bitan G, MacKenzie-Graham A, Jiang L, Hinman JD
Acta Neuropathol Commun. 2019 Aug 20;7(1):135. doi: 10.1186/s40478-019-0783-6.

Autophagy inhibition elicits emergence from metastatic dormancy by inducing and stabilizing Pfkfb3 expression
La Belle Flynn A, Calhoun BC, Sharma A, Chang JC, Almasan A, Schiemann WP
Nat Commun. 2019 Aug 14;10(1):3668. doi: 10.1038/s41467-019-11640-9.

Proteomics, Lipidomics, Metabolomics

Laser Microdissection for Spatial Proteomics of Postmortem Brain Tissue
Hondius DC, Hoozemans JJM, Rozemuller AJM, Li KW, Smit AB
Neuroproteomics, 2019, Springer New York CY

MASS SPECTROMETRY-BASED PROTEOME PROFILE MAY BE USEFUL TO DIFFERENTIATE ADENOID CYSTIC CARCINOMA FROM POLYMORPHOUS ADENOCARCINOMA OF SALIVARY GLANDS,
Fonseca FP, Macedo CCS, dos Santos Costa FS, Leme AFP, Rodrigues RR, Pontes HAR, Altemani A, van Heerden WFP, Martins MD, de Almeida OP, Silva ARS, Lopes MA, Vargas PA
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, 2019 Aug 1, ISSN 2212-4403

Lewy pathology in Parkinson's disease consists of crowded organelles and lipid membranes
Shahmoradian SH, Lewis AJ, Genoud C, Hench J, Moors TE, Navarro PP, Castaño-Díez D, Schweighauser G, Graff-Meyer A, Goldie KN, Sütterlin R, Huisman E, Ingrassia A, Gier Y, Rozemuller AJM, Wang J, Paepe A, Erny J, Staempfli A, Hoernschemeyer J, Großerüschkamp F, Niedieker D, El-Mashtoly SF, Quadri M, Van IJcken WFJ, Bonifati V, Gerwert K, Bohrmann B, Frank S, Britschgi M, Stahlberg H, Van de Berg WDJ, Lauer ME
Nat Neurosci. 2019 Jul;22(7):1099-1109. doi: 10.1038/s41593-019-0423-2.

Fluorescence Applications

Coordination of Neuron Production in Mouse and Human Cerebral Cortex by the Homolog of Drosophila Mastermind Protein
Ayoub AE, Dominguez MH, Benoit J, Ortega JA, Radonjic N, Zecevic N, Rakic P
Brain Behav Evol. 2019 Aug 15;93(2-3):152-165. doi: 10.1159/000500494.

Self-seeding circulating tumor cells promote the proliferation and metastasis of human osteosarcoma by upregulating interleukin-8
Liu T, Ma Q, Zhang Y, Wang X, Xu K, Yan K, Dong W, Fan Q, Zhang Y, Qiu X
Cell Death Dis. 2019 Jul 31;10(8):575. doi: 10.1038/s41419-019-1795-7.

In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9
Steinecke A, Kurabayashi N, Hayano Y, Ishino Y, Taniguchi H
Cell Rep. 2019 Jul 9;28(2):325-331.e4. doi: 10.1016/j.celrep.2019.06.038.

Cancer Research

Differences in gene expression between high-grade dysplasia and invasive HPV+ and HPV- tonsillar and base of tongue cancer
Haeggblom L, Ährlund-Richter A, Mirzaie L, Farrajota Neves da Silva P, Ursu RG, Ramqvist T, Näsman A
Cancer Med. 2019 Aug 27. doi: 10.1002/cam4.2450.

High expression of calreticulin indicates poor prognosis and modulates cell migration and invasion via activating Stat3 in nasopharyngeal carcinoma
Han Y, Liao Q, Wang H, Rao S, Yi P, Tang L, Tian Y, Oyang L, Wang H, Shi Y, Zhou Y
Journal of Cancer, 2019; 10(22): 5460-5468

Autophagy inhibition elicits emergence from metastatic dormancy by inducing and stabilizing Pfkfb3 expression
La Belle Flynn A, Calhoun BC, Sharma A, Chang JC, Almasan A, Schiemann WP
Nat Commun. 2019 Aug 14;10(1):3668. doi: 10.1038/s41467-019-11640-9.

Neurosciences

The median eminence as the hypothalamic area involved in rapid transfer of glucose to the brain: functional and cellular mechanisms
Martínez F, Cifuentes M, Tapia JC, Nualart F
J Mol Med (Berl). 2019 Aug;97(8):1085-1097. doi: 10.1007/s00109-019-01799-5.

Glutamate controls vessel-associated migration of GABA interneurons from the pial migratory route via NMDA receptors and endothelial protease activation
Léger C, Dupré N, Aligny C, Bénard M, Lebon A, Henry V, Hauchecorne M, Galas L, Frebourg T, Leroux P, Vivien D, Lecointre M, Marret S, Gonzalez BJ
Cell Mol Life Sci. 2019 Aug 7. doi: 10.1007/s00018-019-03248-5.

In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9
Steinecke A, Kurabayashi N, Hayano Y, Ishino Y, Taniguchi H
Cell Rep. 2019 Jul 9;28(2):325-331.e4. doi: 10.1016/j.celrep.2019.06.038.

Clinical Applications

Dissecting the genetic basis of focal cortical dysplasia: a large cohort study
Baldassari S, Ribierre T, Marsan E, Adle-Biassette H, Ferrand-Sorbets S, Bulteau C, Dorison N, Fohlen M, Polivka M, Weckhuysen S, Dorfmüller G, Chipaux M, Baulac S
Acta Neuropathol. 2019 Aug 23. doi: 10.1007/s00401-019-02061-5.

Sinus node-like pacemaker mechanisms regulate ectopic pacemaker activity in the adult rat atrioventricular ring
Logantha SJRJ, Kharche SR, Zhang Y, Atkinson AJ, Hao G, Boyett MR, Dobrzynski H
Sci Rep. 2019 Aug 13;9(1):11781. doi: 10.1038/s41598-019-48276-0.

Ischemic axonal injury up-regulates MARK4 in cortical neurons and primes tau phosphorylation and aggregation
Hayden EY, Putman J, Nunez S, Shin WS, Oberoi M, Charreton M, Dutta S, Li Z, Komuro Y, Joy MT, Bitan G, MacKenzie-Graham A, Jiang L, Hinman JD
Acta Neuropathol Commun. 2019 Aug 20;7(1):135. doi: 10.1186/s40478-019-0783-6.

Developmental Research, Epigenetics & Aging

Coordination of Neuron Production in Mouse and Human Cerebral Cortex by the Homolog of Drosophila Mastermind Protein
Ayoub AE, Dominguez MH, Benoit J, Ortega JA, Radonjic N, Zecevic N, Rakic P
Brain Behav Evol. 2019 Aug 15;93(2-3):152-165. doi: 10.1159/000500494.

Uterine spiral artery muscle dedifferentiation
Robson A, Lash GE, Innes BA, Zhang JY, Robson SC, Bulmer JN
Hum Reprod. 2019 Jul 26. pii: dez124. doi: 10.1093/humrep/dez124.

Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
Ma P, Song NN, Li Y, Zhang Q, Zhang L, Zhang L, Kong Q, Ma L, Yang X, Ren B, Li C, Zhao X, Li Y, Xu Y, Gao X, Ding YQ, Mao B
Cell Rep. 2019 Jul 9;28(2):541-553.e4. doi: 10.1016/j.celrep.2019.06.017.

Plant Research

Tissue-Specific Metabolite Profiling on the Different Parts of Bolting and Unbolting Peucedanum praeruptorum Dunn (Qianhu) by Laser Microdissection Combined with UPLC-Q/TOF⁻MS and HPLC⁻DAD
Chen LL, Chu SS, Zhang L, Xie J, Dai M, Wu X, Peng HS
Molecules. 2019 Apr 11;24(7). pii: E1439. doi: 10.3390/molecules24071439.

Transcriptome landscape of the early Brassica napus seed
Ziegler DJ, Khan D, Kalichuk JL, Becker MG, Belmonte MF
J Integr Plant Biol. 2019 Apr 2. doi: 10.1111/jipb.12812.

Transcriptome analysis of Rlm2-mediated host immunity in the Brassica napus-Leptosphaeria maculans pathosystem
Becker MG, Haddadi P, Wan J, Adam L, Walker P, Larkan N, Daayf F, Borhan MH, Belmonte MF
Mol Plant Microbe Interact. 2019 Apr 2. doi: 10.1094/MPMI-01-19-0028-R.

Forensics

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Protectiverole of berberine on ulcerative colitis through modulating enteric glial cells-intestinal epithelial cells-immune cells interactions
Li H, Fan C, Lu H, Feng C, He P, Yang X, Xiang C, Zuo J, Tang W
Acta Pharmaceutica Sinica B, 17 Aug 2019, S2211-3835(19)30637-9

Systematic evaluation of repeatability of IR-MALDESI-MS and normalization strategies for correcting the analytical variation and improving image quality
Tu A, Muddiman DC
Anal Bioanal Chem. 2019 Sep;411(22):5729-5743. doi: 10.1007/s00216-019-01953-5.

Ectosymbiotic bacteria at the origin of magnetoreception in a marine protist
Monteil CL, Vallenet D, Menguy N, Benzerara K, Barbe V, Fouteau S, Cruaud C, Floriani M, Viollier E, Adryanczyk G, Leonhardt N, Faivre D, Pignol D, López-García P, Weld RJ, Lefevre CT
Nat Microbiol. 2019 Jul;4(7):1088-1095. doi: 10.1038/s41564-019-0432-7.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.