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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Table of Content

DNA Research

Detection of human parvovirus B19 infection in the thymus of patients with thymic hyperplasia-associated myasthenia gravis
Gong L, Li Y, Li X, Tu Q, Mou X, Wang S, Wan Y, Lu Q, Wang J, Zhang W, Zhu S, Han X, Yao L, Zhang J, Huang G, Zhang W
Clin Microbiol Infect. 2018 Apr 9. pii: S1198-743X(18)30298-2. doi: 10.1016/j.cmi.2018.03.036.

NanoVelcro rare-cell assays for detection and characterization of circulating tumor cells
Jan YJ, Chen JF, Zhu Y, Lu YT, Chen SH, Chung H, Smalley M, Huang YW, Dong J, Chen LC, Yu HH, Tomlinson JS, Hou S, Agopian VG, Posadas EM, Tseng HR
Adv Drug Deliv Rev. 2018 Mar 15. pii: S0169-409X(18)30045-0. doi: 10.1016/j.addr.2018.03.006.

Esophageal squamous cell carcinoma with low mitochondrial copy number has mesenchymal and stem-like characteristics, and contributes to poor prognosis
Masuike Y, Tanaka K, Makino T, Yamasaki M, Miyazaki Y, Takahashi T, Kurokawa Y, Nakajima K, Mori M, Doki Y
PLoS One. 2018 Feb 15;13(2):e0193159. doi: 10.1371/journal.pone.0193159. eCollection 2018.
http://jem.rupress.org/cgi/pmidlookup?view=long&pmid=29439001

RNA Research

Urinary miR-196a predicts disease progression in patients with chronic kidney disease
Zhang C, Liang S, Cheng S, Li W, Wang X, Zheng C, Zeng C, Shi S, Xie L, Zen K, Liu Z
J Transl Med. 2018 Apr 10;16(1):91. doi: 10.1186/s12967-018-1470-2.

Micro RNAs are involved in activation of epicardium during zebrafish heart regeneration
Ceci M, Carlantoni C, Missinato MA, Bonvissuto D, Di Giacomo B, Contu R, Romano N
Cell Death Discov. 2018 Mar 12;4:41. doi: 10.1038/s41420-018-0041-x. eCollection 2018.

Transcriptional regulation of macrophage cholesterol efflux and atherogenesis by a long noncoding RNA
Sallam T, Jones M, Thomas BJ, Wu X, Gilliland T, Qian K, Eskin A, Casero D, Zhang Z, Sandhu J, Salisbury D, Rajbhandari P, Civelek M, Hong C, Ito A, Liu X, Daniel B, Lusis AJ, Whitelegge J, Nagy L, Castrillo A, Smale S, Tontonoz P
Nat Med. 2018 Feb 12. doi: 10.1038/nm.4479.

Proteomics

Liver zonation index of drug transporter and metabolizing enzyme protein expressions in mouse liver acinus
Tachikawa M, Sumiyoshiya Y, Saigusa D, Sasaki K, Watanabe M, Uchida Y, Terasaki T
Drug Metab Dispos. 2018 Mar 5. pii: dmd.117.079244. doi: 10.1124/dmd.117.079244.

Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells
Zhu Y, Piehowski PD, Zhao R, Chen J, Shen Y, Moore RJ, Shukla AK, Petyuk VA, Campbell-Thompson M, Mathews CE, Smith RD, Qian WJ, Kelly RTNat Commun. 2018 Feb 28;9(1):882. doi: 10.1038/s41467-018-03367-w.

Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer's Disease Tissue Using Laser-Capture Microdissection for Downstream Proteomics
Drummond E, Nayak S, Pires G, Ueberheide B, Wisniewski T
Methods Mol Biol. 2018;1723:319-334. doi: 10.1007/978-1-4939-7558-7_18

Fluorescence Applications

Upregulation of HDAC2 in Laser Capture Nigral Microglia in Parkinson’s Disease
Tan Y, Delvaux E, Nolz J, Coleman PD, Chen S, Mastroeni D
Neurobiology of Aging, 2018, ISSN 0197-4580, doi.org/10.1016/j.neurobiolaging.2018.02.018.

Liver zonation index of drug transporter and metabolizing enzyme protein expressions in mouse liver acinus
Tachikawa M, Sumiyoshiya Y, Saigusa D, Sasaki K, Watanabe M, Uchida Y, Terasaki T 
Drug Metab Dispos. 2018 Mar 5. pii: dmd.117.079244. doi: 10.1124/dmd.117.079244.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.

Cancer Research

The Colorectal Cancer Microenvironment: Strategies for Studying the Role of Cancer-Associated Fibroblasts
Bhome R, Mellone M, Emo K, Thomas GJ, Sayan AE, Mirnezami AH
Methods Mol Biol. 2018;1765:87-98. doi: 10.1007/978-1-4939-7765-9_6.

NanoVelcro rare-cell assays for detection and characterization of circulating tumor cells
Jan YJ, Chen JF, Zhu Y, Lu YT, Chen SH, Chung H, Smalley M, Huang YW, Dong J, Chen LC, Yu HH, Tomlinson JS, Hou S, Agopian VG, Posadas EM, Tseng HR
Adv Drug Deliv Rev. 2018 Mar 15. pii: S0169-409X(18)30045-0. doi: 10.1016/j.addr.2018.03.006.

Esophageal squamous cell carcinoma with low mitochondrial copy number has mesenchymal and stem-like characteristics, and contributes to poor prognosis
Masuike Y, Tanaka K, Makino T, Yamasaki M, Miyazaki Y, Takahashi T, Kurokawa Y, Nakajima K, Mori M, Doki Y
PLoS One. 2018 Feb 15;13(2):e0193159. doi: 10.1371/journal.pone.0193159. eCollection 2018.

Neurosciences

Upregulation of HDAC2 in Laser Capture Nigral Microglia in Parkinson’s Disease
Tan Y, Delvaux E, Nolz J, Coleman PD, Chen S, Mastroeni D
Neurobiology of Aging, 2018, ISSN 0197-4580, doi.org/10.1016/j.neurobiolaging.2018.02.018.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.

Olfactory receptors are expressed in pancreatic β-cells and promote glucose-stimulated insulin secretion
Munakata Y, Yamada T, Imai J, Takahashi K, Tsukita S, Shirai Y, Kodama S, Asai Y, Sugisawa T, Chiba Y, Kaneko K, Uno K, Sawada S, Hatakeyama H, Kanzaki M, Miyazaki JI, Oka Y, Katagiri H
Sci Rep. 2018 Jan 24;8(1):1499. doi: 10.1038/s41598-018-19765-5.

Clinical Applications

Urinary miR-196a predicts disease progression in patients with chronic kidney disease
Zhang C, Liang S, Cheng S, Li W, Wang X, Zheng C, Zeng C, Shi S, Xie L, Zen K, Liu Z
J Transl Med. 2018 Apr 10;16(1):91. doi: 10.1186/s12967-018-1470-2.

Detection of human parvovirus B19 infection in the thymus of patients with thymic hyperplasia-associated myasthenia gravis
Gong L, Li Y, Li X, Tu Q, Mou X, Wang S, Wan Y, Lu Q, Wang J, Zhang W, Zhu S, Han X, Yao L, Zhang J, Huang G, Zhang W
Clin Microbiol Infect. 2018 Apr 9. pii: S1198-743X(18)30298-2. doi: 10.1016/j.cmi.2018.03.036.

Micro RNAs are involved in activation of epicardium during zebrafish heart regeneration
Ceci M, Carlantoni C, Missinato MA, Bonvissuto D, Di Giacomo B, Contu R, Romano N
Cell Death Discov. 2018 Mar 12;4:41. doi: 10.1038/s41420-018-0041-x. eCollection 2018.

Developmental Research, Epigenetics & Aging

MiR-142-3p Inhibits TGF-β3-Induced Blood-Testis Barrier Impairment by Targeting Lethal Giant Larvae Homolog 2
Xu Y, Wu W, Fan Y, Jiang S, Jia X, Su W
Cell Physiol Biochem. 2018 Mar 22;46(1):253-268. doi: 10.1159/000488427.

Laminin-332 regulates differentiation of human interfollicular epidermal stem cells
Yamada T, Hasegawa S, Miyachi K, Date Y, Inoue Y, Yagami A, Arima M, Iwata Y, Yamamoto N, Nakata S, Matsunaga K, Sugiura K, Akamatsu H
Mech Ageing Dev. 2018 Mar 16. pii: S0047-6374(17)30259-2. doi: 10.1016/j.mad.2018.03.007.

Comparative gene expression profiles of dental follicle at different stages of periodontal development: Combined use of laser capture microdissection and microarray
Dang P, Tang Q, Nie M-Y, An Y, Dong R, Hua X-D, Jung H-S, Shi S-G
Journal of Oral Biosciences, doi.org/10.1016/j.job.2018.02.003

Plant Research

Host- and stage-dependent secretome of the arbuscular mycorrhizal fungus Rhizophagus irregularis
Zeng T, Holmer R, Hontelez J, Te Lintel-Hekkert B, Marufu L, de Zeeuw T, Wu F, Schijlen E, Bisseling T, Limpens E
Plant J. 2018 Mar 23. doi: 10.1111/tpj.13908.

Morphology of glandular trichomes of Japanese catnip (Schizonepeta tenuifolia Briquet) and developmental dynamics of their secretory activity
Liu C, Srividya N, Parrish AN, Yue W, Shan M, Wu Q, Lange BM
Phytochemistry. 2018 Mar 10;150:23-30. doi: 10.1016/j.phytochem.2018.02.018.

Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis
Zhang S, Thakare D, Yadegari R
Methods Mol Biol. 2018;1676:153-163. doi: 10.1007/978-1-4939-7315-6_9.

Forensics

DNA perspectives of fixed and paraffin embedded human tissues as resource materials for the identification
Ullah S, Garg RK, Noor F
Egyptian Journal of Forensic Sciences (2017) 7:23

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017

Forensic Biology – Expert Evidence
Gunn P, Roebuck H, Summerell A
Bookchapter, reviewed March 2017

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

Differences in the accumulation of phosphorus between vegetative cells and heterocysts in the cyanobacterium Nodularia spumigena
Braun PD, Schulz-Vogt HN, Vogts A, Nausch M
Sci Rep. 2018 Apr 4;8(1):5651. doi: 10.1038/s41598-018-23992-1.

Characterization of a novel miniaturized burst-mode infrared laser system for IR-MALDESI mass spectrometry imaging
Ekelöf M, Manni J Sr, Nazari M, Bokhart M, Muddiman DC
Anal Bioanal Chem. 2018 Feb 17. doi: 10.1007/s00216-018-0918-9.

Development of vaccine for dyslipidemia targeted to a proprotein convertase subtilisin/kexin type 9 (PCSK9) epitope in mice
Kawakami R, Nozato Y, Nakagami H, Ikeda Y, Shimamura M, Yoshida S, Sun J, Kawano T, Takami Y, Noma T, Rakugi H, Minamino T, Morishita R
PLoS One. 2018 Feb 13;13(2):e0191895. doi: 10.1371/journal.pone.0191895. eCollection 2018.

Live Cell Culture

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.