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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Table of Content

DNA Research

Rapid detection of EGFR mutations in decalcified lung cancer bone metastasis
Boureille A, Ferraro-Peyret C, Pontarollo G, Confavreux C, Pialat J-B, Isaac S, Forest F, Yvorel V,  Watkin E, Girard N, Brevet M
Journal of Bone Oncology, Volume 21, 2020, 100277, ISSN 2212-1374,

The Indian cobra reference genome and transcriptome enables comprehensive identification of venom toxins.
Suryamohan K, Krishnankutty SP, Guillory J, Jevit M, Schröder MS, Wu M, Kuriakose B, Mathew OK, Perumal RC, Koludarov I, Goldstein LD, Senger K, Dixon MD, Velayutham D, Vargas D, Chaudhuri S, Muraleedharan M, Goel R, Chen YJ, Ratan A, Liu P, Faherty B, de la Rosa G, Shibata H, Baca M, Sagolla M, Ziai J, Wright GA, Vucic D, Mohan S, Antony A, Stinson J, Kirkpatrick DS, Hannoush RN, Durinck S, Modrusan Z, Stawiski EW, Wiley K, Raudsepp T, Kini RM, Zachariah A, Seshagiri S
Nat Genet. 2020 Jan 6. doi: 10.1038/s41588-019-0559-8.

Methods for mapping 3D chromosome architecture
Kempfer R, Pombo A
Nat Rev Genet. 2019 Dec 17. doi: 10.1038/s41576-019-0195-2.

RNA Research

Plant-Associated Microbes Alter Root Growth by Modulating Root Apical Meristem
Hussain A, Husna, Ullah I, Naseem M
Methods Mol Biol. 2020;2094:49-58. doi: 10.1007/978-1-0716-0183-9_6

Thyroid hormone inhibits hepatocellular carcinoma progression via induction of differentiation and metabolic reprogramming
Kowalik MA, Puliga E, Cabras L, Sulas P, Petrelli A, Perra A, Ledda-Columbano GM, Morandi A, Merlin S, Orrù C, Sanchez-Martin C, Fornari F, Gramantieri L, Parri M, Rasola A, Bellomo SE, Sebastian C, Follenzi A, Giordano S, Columbano A
J Hepatol. 2020 Jan 15. pii: S0168-8278(20)30009-X. doi: 10.1016/j.jhep.2019.12.018.

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
Wu M, Kriti D, van Bakel H, Jabs EW, Holmes G
J Vis Exp. 2019 Dec 18;(154). doi: 10.3791/60503.

Proteomics, Lipidomics, Metabolomics

Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Cahill JF, Kertesz V
Methods Mol Biol. 2020;2064:89-101. doi: 10.1007/978-1-4939-9831-9_7.

Proteomics of Cytochrome c Oxidase-Negative versus -Positive Muscle Fiber Sections in Mitochondrial Myopathy
Murgia M, Tan J, Geyer PE, Doll S, Mann M, Klopstock T
Cell Reports, Volume 29, Issue 12, 17 Dec 2019, Pages 3825-3834.e4

Jupiter microtubule-associated homolog 1 (JPT1): A predictive and pharmacodynamic biomarker of metformin response in endometrial cancers
Bateman NW, Teng PN, Hope E, Hood BL, Oliver J, Ao W, Zhou M, Wang G, Tommarello D, Wilson K, Litzy T, Conrads KA, Hamilton CA, Darcy KM, Casablanca Y, Maxwell GL, Bae-Jump V, Conrads TP
Cancer Med. 2019 Dec 6. doi: 10.1002/cam4.2729.

Fluorescence Applications

Cav2.3 channels contribute to dopaminergic neuron loss in a model of Parkinson’s disease
Benkert J, Hess S, Roy S, Beccano-Kelly D, Wiederspohn N, Duda J, Simons C, Patil K, Gaifullina A,  Mannal N, Dragicevic E, Spaich D, Müller S, Nemeth J, Hollmann H, Deuter N, Mousba Y, Kubisch C,
Poetschke C, Striessnig J, Pongs O, Schneider T, Wade-Martins R, Patel S, Parlato R, Frank T, Kloppenburg P & Liss B
Nat Commun 10, 5094 (8 Nov 2019) doi:10.1038/s41467-019-12834-x

Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues
Skalicky S, Zwiers PJ, Kuiper T, Schraml E, Hackl M, Molema G
Biotechniques. 2019 Oct 17. doi: 10.2144/btn-2019-0032.

Circulating uromodulin inhibits systemic oxidative stress by inactivating the TRPM2 channel
LaFavers KA, Macedo E, Garimella PS, Lima C, Khan S, Myslinski J, McClintick J, Witzmann FA, Winfree S, Phillips CL, Hato T, Dagher PC, Wu XR, El-Achkar TM, Micanovic R
Sci Transl Med. 2019 Oct 2;11(512). pii: eaaw3639. doi: 10.1126/scitranslmed.aaw3639.

Cancer Research

Rapid detection of EGFR mutations in decalcified lung cancer bone metastasis
Boureille A, Ferraro-Peyret C, Pontarollo G, Confavreux C, Pialat J-B, Isaac S, Forest F, Yvorel V,  Watkin E, Girard N, Brevet M
Journal of Bone Oncology, Volume 21, 2020, 100277, ISSN 2212-1374,

SIRPB1 promotes prostate cancer cell proliferation via Akt activation
Song Q, Qin S, Pascal LE, Zou C, Wang W, Tong H, Zhang J, Catalona WJ, Dhir R, Morrell M, Balasubramani GK, Lu Y, Wang Z
Prostate. 2020 Jan 6. doi: 10.1002/pros.23950.

Uterine adenomyosis is an oligoclonal disorder associated with KRAS mutations
Inoue S, Hirota Y, Ueno T, Fukui Y, Yoshida E, Hayashi T, Kojima S, Takeyama R, Hashimoto T, Kiyono T, Ikemura M, Taguchi A, Tanaka T, Tanaka Y, Sakata S, Takeuchi K, Muraoka A, Osuka S, Saito T, Oda K, Osuga Y, Terao Y, Kawazu M, Mano H
Nat Commun. 2019 Dec 19;10(1):5785. doi: 10.1038/s41467-019-13708-y.

Neurosciences

Neurochemical Characterization of Neurons Expressing Estrogen Receptor β in the Hypothalamic Nuclei of Rats Using in Situ Hybridization and Immunofluorescence
Kanaya M, Higo S, Ozawa H
Int J Mol Sci. 2019 Dec 23;21(1). pii: E115. doi: 10.3390/ijms21010115.

The Western Diet Regulates Hippocampal Microvascular Gene Expression: An Integrated Genomic Analyses in Female Mice.
Nuthikattu S, Milenkovic D, Rutledge J, Villablanca A
Sci Rep. 2019 Dec 13;9(1):19058. doi: 10.1038/s41598-019-55533-9.

Cav2.3 channels contribute to dopaminergic neuron loss in a model of Parkinson’s disease
Benkert J, Hess S, Roy S, Beccano-Kelly D, Wiederspohn N, Duda J, Simons C, Patil K, Gaifullina A,  Mannal N, Dragicevic E, Spaich D, Müller S, Nemeth J, Hollmann H, Deuter N, Mousba Y, Kubisch C,
Poetschke C, Striessnig J, Pongs O, Schneider T, Wade-Martins R, Patel S, Parlato R, Frank T, Kloppenburg P & Liss B
Nat Commun 10, 5094 (8 Nov 2019) doi:10.1038/s41467-019-12834-x

Clinical Applications

Regional Differences in Tight Junction Protein Expression in the Blood–DRG Barrier and Their Alterations after Nerve Traumatic Injury in Rats
Lux TJ, Hu X, Ben-Kraiem A, Blum R, Tsung-Chieh Chen J, Rittner HL
Int. J. Mol. Sci. 2020, 21, 270; doi:10.3390/ijms21010270

Neural EGFL like 1 as a potential pro-chondrogenic, anti-inflammatory dual-functional disease-modifying osteoarthritis drug
Li C, Zheng Z, Ha P, Jiang W, Berthiaume EA, Lee S, Mills Z, Pan H, Chen EC, Jiang J, Culiat CT, Zhang X, Ting K, Soo C
Biomaterials, Volume 226, January 2020, 119541

Proteomics of Cytochrome c Oxidase-Negative versus -Positive Muscle Fiber Sections in Mitochondrial Myopathy
Murgia M, Tan J, Geyer PE, Doll S, Mann M, Klopstock T
Cell Reports, Volume 29, Issue 12, 17 Dec 2019, Pages 3825-3834.e4

Developmental Research, Epigenetics & Aging

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
Wu M, Kriti D, van Bakel H, Jabs EW, Holmes G
J Vis Exp. 2019 Dec 18;(154). doi: 10.3791/60503.

Prenatal electroporation-mediated gene transfer restores Slc26a4 knock-out mouse hearing and vestibular function
Takeda H, Miwa T, Kim MY, Choi BY, Orita Y, Minoda R
Sci Rep. 2019 Nov 29;9(1):17979. doi: 10.1038/s41598-019-54262-3.

Spatiotemporally Dependent Vascularization Is Differently Utilized among Neural Progenitor Subtypes during Neocortical Development
Komabayashi-Suzuki M, Yamanishi E, Watanabe C, Okamura M, Tabata H, Iwai R, Ajioka I, Matsushita J, Kidoya H, Takakura N, Okamoto T, Kinoshita K, Ichihashi M, Nagata KI, Ema M, Mizutani KI
Cell Rep. 2019 Oct 29;29(5):1113-1129.e5. doi: 10.1016/j.celrep.2019.09.048.

Plant Research

Plant-Associated Microbes Alter Root Growth by Modulating Root Apical Meristem
Hussain A, Husna, Ullah I, Naseem M
Methods Mol Biol. 2020;2094:49-58. doi: 10.1007/978-1-0716-0183-9_6

Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Cahill JF, Kertesz V
Methods Mol Biol. 2020;2064:89-101. doi: 10.1007/978-1-4939-9831-9_7.

A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms
Kivivirta K, Herbert D, Lange M, Beuerlein K, Altmüller J, Becker A
Plant Methods. 2019 Dec 16;15:151. doi: 10.1186/s13007-019-0536-3. eCollection 2019.

Forensics

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

The Indian cobra reference genome and transcriptome enables comprehensive identification of venom toxins.
Suryamohan K, Krishnankutty SP, Guillory J, Jevit M, Schröder MS, Wu M, Kuriakose B, Mathew OK, Perumal RC, Koludarov I, Goldstein LD, Senger K, Dixon MD, Velayutham D, Vargas D, Chaudhuri S, Muraleedharan M, Goel R, Chen YJ, Ratan A, Liu P, Faherty B, de la Rosa G, Shibata H, Baca M, Sagolla M, Ziai J, Wright GA, Vucic D, Mohan S, Antony A, Stinson J, Kirkpatrick DS, Hannoush RN, Durinck S, Modrusan Z, Stawiski EW, Wiley K, Raudsepp T, Kini RM, Zachariah A, Seshagiri S
Nat Genet. 2020 Jan 6. doi: 10.1038/s41588-019-0559-8.

Aluminum-induced toxicity in Urochloa brizantha genotypes: A first glance into root Al-apoplastic and -symplastic compartmentation, Al-translocation and antioxidant performance
Furlan F, Borgo L, Silveira Rabêlo FH, Lanzoni Rossi M, Scaglia Linhares F, Pinheiro Martinelli A, Antunes Azevedo R, Lavres J
Chemosphere, Volume 243, March 2020, 125362

Methods for mapping 3D chromosome architecture
Kempfer R, Pombo A
Nat Rev Genet. 2019 Dec 17. doi: 10.1038/s41576-019-0195-2.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.