DNA Research
Frequent SLC35A2 brain mosaicism in mild malformation of cortical development with oligodendroglial hyperplasia in epilepsy (MOGHE)
Bonduelle T, Hartlieb T, Baldassari S, Sim NS, Kim SH, Kang HC, Kobow K, Coras R, Chipaux M, Dorfmüller G, Adle-Biassette H, Aronica E, Lee JH, Blumcke I, Baulac S
Acta Neuropathol Commun. 2021 Jan 6;9(1):3. doi: 10.1186/s40478-020-01085-3.
Endometrial cancer with a POLE mutation progresses frequently through the type I pathway despite its high-grade endometrioid morphology: a cohort study at a single institution in Japan
Monsur M, Yamaguchi M, Tashiro H, Yoshinobu K, Saito F, Erdenebaatar C, Li C, Iwagoi Y, Ohba T, Iyama KI, Katabuchi H
Med Mol Morphol. 2021 Jan 5. doi: 10.1007/s00795-020-00273-3.
Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing
Ellis P, Moore L, Sanders MA, Butler TM, Brunner SF, Lee-Six H, Osborne R, Farr B, Coorens THH, Lawson ARJ, Cagan A, Stratton MR, Martincorena I, Campbell PJ
Nat Protoc. 2020 Dec 14. doi: 10.1038/s41596-020-00437-6.
RNA Research
CcLBD25 functions as a key regulator of haustorium development in Cuscuta campestris
Jhu M-Y, Ichihashi Y, Farhi M, Wong C, Sinha NR
bioRxiv, Jan 2021, doi: doi.org/10.1101/2021.01.04.425251
Microglial inflammation after chronic spinal cord injury is enhanced by reactive astrocytes via the fibronectin/β1 integrin pathway
Yoshizaki S, Tamaru T, Hara M, Kijima K, Tanaka M, Konno DJ, Matsumoto Y, Nakashima Y, Okada S
J Neuroinflammation. 2021 Jan 6;18(1):12. doi: 10.1186/s12974-020-02059-x.
A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots
Mounier T, Navarro-Sanz S, Bureau C, Antoine L, Varoquaux F, Durandet F, Périn C
BMC Mol Cell Biol. 2020 Dec 10;21(1):92. doi: 10.1186/s12860-020-00312-y.
Proteomics, Lipidomics, Metabolomics
Mass Spectrometry Spatial-Omics on a Single Conductive Slide
Mezger STP, Mingels AMA, Bekers O, Heeren RMA, Cillero-Pastor B
Anal Chem. 2021 Jan 7. doi: 10.1021/acs.analchem.0c04572.
Demineralization and sectioning of human kidney stones: A molecular investigation revealing the spatial heterogeneity of the stone matrix
Canela VH, Bledsoe SB, Lingeman JE, Gerber G, Worcester EM, El-Achkar TM, Williams JC Jr
Physiol Rep. 2021 Jan;9(1):e14658. doi: 10.14814/phy2.14658.
Comparative analysis of differentially abundant proteins quantified by LC–MS/MS between flash frozen and laser microdissected OCT-embedded breast tumor samples
Sturtz LA, Wang G, Shah P, Searfoss R, Raj-Kumar P-K, Hooke JA, Fantacone-Campbell JL, Deyarmin B, Cutler ML, Sarangarajan R, Narain NR, Kiebish MA, Kovatich AJ, Shriver CD
Clin Proteom 17, 40 (7 Nov 2020).
Fluorescence Applications
Microglial inflammation after chronic spinal cord injury is enhanced by reactive astrocytes via the fibronectin/β1 integrin pathway
Yoshizaki S, Tamaru T, Hara M, Kijima K, Tanaka M, Konno DJ, Matsumoto Y, Nakashima Y, Okada S
J Neuroinflammation. 2021 Jan 6;18(1):12. doi: 10.1186/s12974-020-02059-x.
Agonistic CD40 antibody therapy induces tertiary lymphoid structures but impairs the response to immune checkpoint blockade in glioma
van Hooren L, Vaccaro A, Ramachandran M, Vazaios K, Libard S, van de Walle T, Georganaki M, Huang H, Pietilä I, Lau J, Ulvmar MH, Karlsson MCI, Zetterling M, Mangsbo SM, Jakola AS, Olsson Bontell T, Smits A, Essand M, Dimberg A
bioRxiv, 06 Jan 2021, doi: doi.org/10.1101/2021.01.05.425377
Transcriptome profiling of laser-captured crown root primordia reveals new pathways activated during early stages of crown root formation in rice.
Lavarenne J, Gonin M, Champion A, Javelle M, Adam H, Rouster J, Conejéro G, Lartaud M, Verdeil JL, Laplaze L, Sallaud C, Lucas M, Gantet P
PLoS One. 2020 Nov 19;15(11):e0238736. doi: 10.1371/journal.pone.0238736. eCollection 2020.
Cancer Research
Genomic Sequencing of Cancer-related Genes in Sinonasal Squamous Cell Carcinoma and Coexisting Inverted Papilloma
Uchi R, Jiromaru R, Yasumatsu R, Yamamoto H, Hongo T, Manako T, Sato K, Hashimoto K, Wakasaki T, Matsuo M, Nakagawa T
Anticancer Res. 2021 Jan;41(1):71-79. doi: 10.21873/anticanres.14752.
Agonistic CD40 antibody therapy induces tertiary lymphoid structures but impairs the response to immune checkpoint blockade in glioma
van Hooren L, Vaccaro A, Ramachandran M, Vazaios K, Libard S, van de Walle T, Georganaki M, Huang H, Pietilä I, Lau J, Ulvmar MH, Karlsson MCI, Zetterling M, Mangsbo SM, Jakola AS, Olsson Bontell T, Smits A, Essand M, Dimberg A
bioRxiv, 06 Jan 2021, doi: doi.org/10.1101/2021.01.05.425377
Endometrial cancer with a POLE mutation progresses frequently through the type I pathway despite its high-grade endometrioid morphology: a cohort study at a single institution in Japan
Monsur M, Yamaguchi M, Tashiro H, Yoshinobu K, Saito F, Erdenebaatar C, Li C, Iwagoi Y, Ohba T, Iyama KI, Katabuchi H
Med Mol Morphol. 2021 Jan 5. doi: 10.1007/s00795-020-00273-3.
Neurosciences
Microglial inflammation after chronic spinal cord injury is enhanced by reactive astrocytes via the fibronectin/β1 integrin pathway
Yoshizaki S, Tamaru T, Hara M, Kijima K, Tanaka M, Konno DJ, Matsumoto Y, Nakashima Y, Okada S
J Neuroinflammation. 2021 Jan 6;18(1):12. doi: 10.1186/s12974-020-02059-x.
Frequent SLC35A2 brain mosaicism in mild malformation of cortical development with oligodendroglial hyperplasia in epilepsy (MOGHE)
Bonduelle T, Hartlieb T, Baldassari S, Sim NS, Kim SH, Kang HC, Kobow K, Coras R, Chipaux M, Dorfmüller G, Adle-Biassette H, Aronica E, Lee JH, Blumcke I, Baulac S
Acta Neuropathol Commun. 2021 Jan 6;9(1):3. doi: 10.1186/s40478-020-01085-3.
Constant Light in Critical Postnatal Days Affects Circadian Rhythms in Locomotion and Gene Expression in the Suprachiasmatic Nucleus, Retina, and Pineal Gland Later in Life
Kubištová A, Spišská V, Petrželková L, Hrubcová L, Moravcová S, Maierová L, Bendová Z
Biomedicines. 2020 Dec 7;8(12):E579. doi: 10.3390/biomedicines8120579.
Clinical Applications
Demineralization and sectioning of human kidney stones: A molecular investigation revealing the spatial heterogeneity of the stone matrix
Canela VH, Bledsoe SB, Lingeman JE, Gerber G, Worcester EM, El-Achkar TM, Williams JC Jr
Physiol Rep. 2021 Jan;9(1):e14658. doi: 10.14814/phy2.14658.
Frequent SLC35A2 brain mosaicism in mild malformation of cortical development with oligodendroglial hyperplasia in epilepsy (MOGHE)
Bonduelle T, Hartlieb T, Baldassari S, Sim NS, Kim SH, Kang HC, Kobow K, Coras R, Chipaux M, Dorfmüller G, Adle-Biassette H, Aronica E, Lee JH, Blumcke I, Baulac S
Acta Neuropathol Commun. 2021 Jan 6;9(1):3. doi: 10.1186/s40478-020-01085-3.
Distribution of amyloidosis subtypes based on tissue biopsy site - Consecutive analysis of 729 patients at a single amyloidosis center in Japan
Abe R, Katoh N, Takahashi Y, Takasone K, Yoshinaga T, Yazaki M, Kametani F, Sekijima Y
Pathol Int. 2020 Oct 28. doi: 10.1111/pin.13041.
Developmental Research, Epigenetics & Aging
Global hypermethylation of intestinal epithelial cells is a hallmark feature of neonatal surgical necrotizing enterocolitis
Good M, Chu T, Shaw P, McClain L, Chamberlain A, Castro C, Rimer JM, Mihi B, Gong Q, Nolan LS, Cooksey K, Linneman L, Agrawal P, Finegold DN, Peters D
Clin Epigenetics. 2020 Dec 11;12(1):190. doi: 10.1186/s13148-020-00983-6.
Runx2 is essential for the transdifferentiation of chondrocytes into osteoblasts
Qin X, Jiang Q, Nagano K, Moriishi T, Miyazaki T, Komori H, Ito K, Mark KV, Sakane C, Kaneko H, Komori T
PLoS Genet. 2020 Nov 30;16(11):e1009169. doi: 10.1371/journal.pgen.1009169.
Uterine epithelial progesterone receptor governs uterine receptivity through epithelial cell differentiation.
Gebril M, Hirota Y, Aikawa S, Fukui Y, Kaku T, Matsuo M, Hirata T, Akaeda S, Hiraoka T, Shimizu-Hirota R, Takeda N, Taha T, Balah OA, Elnoury MAH, Fujii T, Osuga Y
Endocrinology. 2020 Oct 25:bqaa195. doi: 10.1210/endocr/bqaa195.
Plant Research
CcLBD25 functions as a key regulator of haustorium development in Cuscuta campestris
Jhu M-Y, Ichihashi Y, Farhi M, Wong C, Sinha NR
bioRxiv, Jan 2021, doi: doi.org/10.1101/2021.01.04.425251
A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots
Mounier T, Navarro-Sanz S, Bureau C, Antoine L, Varoquaux F, Durandet F, Périn C
BMC Mol Cell Biol. 2020 Dec 10;21(1):92. doi: 10.1186/s12860-020-00312-y.
Spatial transcriptional signatures define margin morphogenesis along the proximal-distal and medio-lateral axes in tomato (Solanum lycopersicum) leaves
Martinez CC, Li S, Woodhouse MR, Sugimoto K, Sinha NR
The Plant Cell, koaa012, doi.org/10.1093/plcell/koaa012
Forensics
Recent trends and developments in forensic DNA extraction
Chong KWY, Thong Z, Kiu-Choong Syn C
WIREs Forensic Science, 30 July 2020, DOI: 10.1002/wfs2.1395
Compatibility of the ForenSeq™ DNA Signature Prep Kit with laser microdissected cells: An exploration of issues that arise with samples containing low cell numbers
England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison SA
Forensic Science International: Genetics, 1 May 2020, 102278
Application of Optical Trapping to Obtain Single-Source STR Profiles fr ofiles from Forensically Rele ensically Relevant Body Fluid Mixtur ant Body Fluid Mixtures with es with Modified DNA Analysis Workflow
O`Brien BJ
Thesis, 28 April 2020
Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.
Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/
Miscellaneous
Mass Spectrometry Spatial-Omics on a Single Conductive Slide
Mezger STP, Mingels AMA, Bekers O, Heeren RMA, Cillero-Pastor B
Anal Chem. 2021 Jan 7. doi: 10.1021/acs.analchem.0c04572.
Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection
Cheng L, Mann SA, Lopez-Beltran A, Chovanec M, Santoni M, Wang M,. Albany C, Adra N, Davidson DD, Cimadamore A, Montironi R, and Zhang S
Methods Mol Biol. 2021;2195:31-47. doi: 10.1007/978-1-0716-0860-9_3.
Therapeutic response monitoring after targeted therapy in an orthotopic rat model of hepatocellular carcinoma using contrast-enhanced ultrasound: Focusing on inter-scanner, and inter-operator reproducibility
Ryu H, Kim JH, Lee S, Han JK
PLoS One. 2020 Dec 23;15(12):e0244304. doi: 10.1371/journal.pone.0244304. eCollection 2020.
Live Cell Culture
Methods for Single-Cell Isolation and Preparation
Pensold D, Zimmer-Bensch G
In: Yu B., Zhang J., Zeng Y., Li L., Wang X. (eds) Single-cell Sequencing and Methylation. Advances in Experimental Medicine and Biology, vol 1255.
Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.
Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.
