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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Table of Content

DNA Research

dPCR Mutational Analyses in Cell-Free DNA: A Comparison with Tissues
Takeshita T, Iwase H
Methods Mol Biol. 2019;1909:105-118. doi: 10.1007/978-1-4939-8973-7_8.

A phenotypic map of disseminated hepatocellular carcinoma suggests clonal constraints in metastatic sites
Martins-Filho SN, Alves VAF, Wakamatsu A, Maeda M, Craig AJ, Assato AK, Villacorta-Martin C, D'Avola D, Labgaa I, Carrilho FJ, Thung SN, Villanueva A
Histopathology. 2019 Jan 12. doi: 10.1111/his.13809.

Rapidly and Slowly Growing Lineages in Chromosomal Instability-Type Gland-Forming Gastric Carcinomas as Revealed by Multisampling Analysis of DNA Copy-Number Profile
Duong TT, Vo DT, Nakayama T, Mukaisho KI, Bamba M, Nguyen TS, Sugihara H
Pathobiology. 2019 Jan 9:1-10. doi: 10.1159/000494926.

RNA Research

Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome
MacFarlane EG, Parker SJ, Shin JY, Ziegler SG, Creamer TJ, Bagirzadeh R, Bedja D, Chen Y, Calderon JF, Weissler K, Frischmeyer-Guerrerio PA, Lindsay ME, Habashi JP, Dietz H
J Clin Invest. 2019 Feb 1;129(2):659-675. doi: 10.1172/JCI123547. Epub 2019 Jan 7.

Thermal stress induces glycolytic beige fat formation via a myogenic state
Chen Y, Ikeda K, Yoneshiro T, Scaramozza A, Tajima K, Wang Q, Kim K, Shinoda K, Sponton CH, Brown Z, Brack A, Kajimura S
Nature. 2019 Jan;565(7738):180-185. doi: 10.1038/s41586-018-0801-z.

Pathological changes of distal motor neurons after complete spinal cord injury
Yokota K, Kubota K, Kobayakawa K, Saito T, Hara M, Kijima K, Maeda T, Katoh H, Ohkawa Y, Nakashima Y, Okada S
Mol Brain. 2019 Jan 9;12(1):4. doi: 10.1186/s13041-018-0422-3.

Proteomics

Spatial and temporal changes in myogenic protein expression by the microenvironment after freeze injury
Yoon N, Chu V, Gould M, Zhang M
J Anat. 2019 Mar;234(3):359-367. doi: 10.1111/joa.12925.

Combination of laser microdissection, 2D-DIGE and MALDI-TOF MS to identify protein biomarkers to predict colorectal cancer spread
Kirana C, Peng L, Miller R, Keating JP, Glenn C, Shi H, Jordan TW, Maddern GJ, Stubbs RS
Clin Proteomics. 2019 Jan 22;16:3. doi: 10.1186/s12014-019-9223-7. eCollection 2019.

AL amyloidosis with non-amyloid forming monoclonal immunoglobulin deposition; a case mimicking AHL amyloidosis
Manabe S, Iwasaki C, Hatano M, Kametani F, Yazaki M, Nitta K, Nagata M
BMC Nephrol. 2018 Nov 22;19(1):337. doi: 10.1186/s12882-018-1050-y.

Fluorescence Applications

Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome
MacFarlane EG, Parker SJ, Shin JY, Ziegler SG, Creamer TJ, Bagirzadeh R, Bedja D, Chen Y, Calderon JF, Weissler K, Frischmeyer-Guerrerio PA, Lindsay ME, Habashi JP, Dietz H
J Clin Invest. 2019 Feb 1;129(2):659-675. doi: 10.1172/JCI123547. Epub 2019 Jan 7.

Effects of nerve growth factor neutralization on TRP channel expression in laser-captured bladder afferent neurons in mice with spinal cord injury
Shimizu N, Wada N, Shimizu T, Suzuki T, Takaoka EI, Kanai AJ, de Groat WC, Hirayama A, Hashimito M, Uemura H, Yoshimura N
Neurosci Lett. 2018 Jun 27. pii: S0304-3940(18)30452-X. doi: 10.1016/j.neulet.2018.06.049.

Upregulation of HDAC2 in Laser Capture Nigral Microglia in Parkinson’s Disease
Tan Y, Delvaux E, Nolz J, Coleman PD, Chen S, Mastroeni D
Neurobiology of Aging, 2018, ISSN 0197-4580, doi.org/10.1016/j.neurobiolaging.2018.02.018.

Cancer Research

NF-κB-induced NOX1 activation promotes gastric tumorigenesis through the expansion of SOX2-positive epithelial cells
Echizen K, Horiuchi K, Aoki Y, Yamada Y, Minamoto T, Oshima H, Oshima M
Oncogene. 2019 Jan 30. doi: 10.1038/s41388-019-0702-0.

High miR-182 Levels Associate with Low-Risk Prostate Cancer
Baumann B, Acosta AM, Richards Z, Deaton R, Sapatynska A, Murphy A, Kajdacsy-Balla A, Gann PH, Nonn L
Am J Pathol. 2019 Jan 28. pii: S0002-9440(18)30384-5. doi: 10.1016/j.ajpath.2018.12.014.

Qualitative Assurance in Molecular Testing: Assessing Minimum Tumour Area Requirements for PCR Amplification and MALDI-TOF MS Using FFPE Tissues
Cumming N, Dinan A, McLean CA
Pathology, 2019

Neurosciences

Neuropilin-1-mediated pruning of corticospinal tract fibers is required for motor recovery after spinal cord injury
Nakanishi T, Fujita Y, Yamashita T
Cell Death Dis. 2019 Jan 25;10(2):67. doi: 10.1038/s41419-019-1338-2.

Pathological changes of distal motor neurons after complete spinal cord injury
Yokota K, Kubota K, Kobayakawa K, Saito T, Hara M, Kijima K, Maeda T, Katoh H, Ohkawa Y, Nakashima Y, Okada S
Mol Brain. 2019 Jan 9;12(1):4. doi: 10.1186/s13041-018-0422-3.

Transcriptomic Landscape of von Economo Neurons in Human Anterior Cingulate Cortex Revealed by Microdissected-Cell RNA Sequencing
Yang L, Yang Y, Yuan J, Sun Y, Dai J, Su B
Cereb Cortex. 2018 Dec 7. doi: 10.1093/cercor/bhy286.

Clinical Applications

Spatial and temporal changes in myogenic protein expression by the microenvironment after freeze injury
Yoon N, Chu V, Gould M, Zhang M
J Anat. 2019 Mar;234(3):359-367. doi: 10.1111/joa.12925.
doi.org/10.1111/joa.12925

Inhibition of Nuclear Factor Kappa B Prevents the Development of Experimental Periapical Lesions
Kaneko T, Myo Zaw SY, Sueyama Y, Katsube KI, Kaneko R, Nör JE, Okiji T
J Endod. 2019 Feb;45(2):168-173. doi: 10.1016/j.joen.2018.10.006.

Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome
MacFarlane EG, Parker SJ, Shin JY, Ziegler SG, Creamer TJ, Bagirzadeh R, Bedja D, Chen Y, Calderon JF, Weissler K, Frischmeyer-Guerrerio PA, Lindsay ME, Habashi JP, Dietz H
J Clin Invest. 2019 Feb 1;129(2):659-675. doi: 10.1172/JCI123547. Epub 2019 Jan 7.

Developmental Research, Epigenetics & Aging

Thermal stress induces glycolytic beige fat formation via a myogenic state
Chen Y, Ikeda K, Yoneshiro T, Scaramozza A, Tajima K, Wang Q, Kim K, Shinoda K, Sponton CH, Brown Z, Brack A, Kajimura S
Nature. 2019 Jan;565(7738):180-185. doi: 10.1038/s41586-018-0801-z.

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Laser microdissection-based tissue specific transcriptome analyses reveals novel regulatory network of genes involved in heat-induced grain chalk in rice endosperm
Ishimaru T, Parween S, Saito Y, Shigemitsu T, Yamakawa H, Nakazono M, Masumura T, Nishizawa NK, Kondo M, Sreenivasulu N
Plant Cell Physiol. 2018 Dec 4. doi: 10.1093/pcp/pcy233.

Plant Research

Cellular‐Specific Detection of Polyphenolic Compounds by NMR‐and MS‐Based Techniques
Hölscher D
Recent Advances in Polyphenol Research, 2019

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Laser microdissection-based tissue specific transcriptome analyses reveals novel regulatory network of genes involved in heat-induced grain chalk in rice endosperm
Ishimaru T, Parween S, Saito Y, Shigemitsu T, Yamakawa H, Nakazono M, Masumura T, Nishizawa NK, Kondo M, Sreenivasulu N
Plant Cell Physiol. 2018 Dec 4. doi: 10.1093/pcp/pcy233.

Forensics

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017

Forensic Biology – Expert Evidence
Gunn P, Roebuck H, Summerell A
Bookchapter, reviewed March 2017

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Dark aerobic sulfide oxidation by anoxygenic phototrophs in anoxic waters
Berg JS, Pjevac P, Sommer T, Buckner CRT, Philippi M, Hach PF, Liebeke M, Holtappels M, Danza F, Tonolla M, Sengupta A, Schubert CJ, Milucka J, Kuypers MMM
Environ Microbiol. 2019 Jan 28. doi: 10.1111/1462-2920.14543.

Comparative localization of cystathionine beta synthases and cystathionine gamma lyase in canine, non-human primate and human retina
Badiei A, Sudharsan R, Santana E, Dunaief JL, Aguirre GD
Exp Eye Res. 2019 Jan 14;181:72-84. doi: 10.1016/j.exer.2019.01.007. [

Single-cell imaging of phosphorus uptake shows that key harmful algae rely on different phosphorus sources for growth
Schoffelen NJ, Mohr W, Ferdelman TG, Littmann S, Duerschlag J, Zubkov MV, Ploug H, Kuypers MMM
Sci Rep. 2018 Nov 21;8(1):17182. doi: 10.1038/s41598-018-35310-w.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.