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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Topics & Tags

Table of Content

DNA Research

Pulmonary melanocytic nevus - A case report with a mutation analysis of common driver oncogenes
Tanaka M, Matsumura M, Okudela K, Mitsui H, Tateishi Y, Umeda S, Suzuki T, Koike C, Kataoka T, Kawano N, Kojima Y, Osawa H, Ohashi K
Pathol Int. 2019 Sep 25. doi: 10.1111/pin.12850.

Long-term Risk of Malignancy in Branch Duct Intraductal Papillary Mucinous Neoplasms
Oyama H, Tada M, Takagi K, Tateishi K, Hamada T, Nakai Y, Hakuta R, Ijichi H, Ishigaki K, Kanai S, Kogure H, Mizuno S, Saito K, Saito T, Sato T, Suzuki T, Takahara N, Morishita Y, Arita J, Hasegawa K, Tanaka M, Fukayama M, Koike K
Gastroenterology. 2019 Aug 29. pii: S0016-5085(19)41257-2. doi: 10.1053/j.gastro.2019.08.032.

In Vivo Single-Cell Genotyping of Mouse Cortical Neurons Transfected with CRISPR/Cas9
Steinecke A, Kurabayashi N, Hayano Y, Ishino Y, Taniguchi H
Cell Rep. 2019 Jul 9;28(2):325-331.e4. doi: 10.1016/j.celrep.2019.06.038.

RNA Research

MicroRNA23a Overexpression in Crohn's Disease Targets Tumour Necrosis Factor Alpha Inhibitor Protein 3, Increasing Sensitivity to TNF and Modifying the Epithelial Barrier
Felwick RK, Dingley GJR, Martinez-Nunez R, Sanchez-Elsner T, Cummings JRF, Collins JE
J Crohns Colitis. 2019 Oct 18. pii: jjz145. doi: 10.1093/ecco-jcc/jjz145.

Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues
Skalicky S, Zwiers PJ, Kuiper T, Schraml E, Hackl M, Molema G
Biotechniques. 2019 Oct 17. doi: 10.2144/btn-2019-0032.

Profiling the unique protective properties of intracranial arterial endothelial cells
Hermkens DMA, Stam OCG, de Wit NM, Fontijn RD, Jongejan A, Moerland PD, Mackaaij C, Waas ISE, Daemen MJAP, de Vries HE
Acta Neuropathol Commun. 2019 Oct 14;7(1):151. doi: 10.1186/s40478-019-0805-4.

Proteomics, Lipidomics, Metabolomics

Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Cahill JF, Kertesz V
Methods Mol Biol. 2020;2064:89-101. doi: 10.1007/978-1-4939-9831-9_7.

Enrichment of histones from patient samples for mass spectrometry-based analysis of post-translational modifications
Noberini R, Restellini C, Oliva Savoia E, Bonaldi T
Methods. 2019 Oct 9. pii: S1046-2023(19)30136-7. doi: 10.1016/j.ymeth.2019.10.001.

Laser Microdissection for Spatial Proteomics of Postmortem Brain Tissue
Hondius DC, Hoozemans JJM, Rozemuller AJM, Li KW, Smit AB
Neuroproteomics, 2019, Springer New York CY

Fluorescence Applications

Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues
Skalicky S, Zwiers PJ, Kuiper T, Schraml E, Hackl M, Molema G
Biotechniques. 2019 Oct 17. doi: 10.2144/btn-2019-0032.

Circulating uromodulin inhibits systemic oxidative stress by inactivating the TRPM2 channel
LaFavers KA, Macedo E, Garimella PS, Lima C, Khan S, Myslinski J, McClintick J, Witzmann FA, Winfree S, Phillips CL, Hato T, Dagher PC, Wu XR, El-Achkar TM, Micanovic R
Sci Transl Med. 2019 Oct 2;11(512). pii: eaaw3639. doi: 10.1126/scitranslmed.aaw3639.

Early pre- and postsynaptic decrease in glutamatergic and cholinergic signaling after spinalization is not modified when stimulating proprioceptive input to the ankle extensor α-motoneurons: Anatomical and neurochemical study
Grycz K, Głowacka A, Ji B, Czarkowska-Bauch J, Gajewska-Woźniak O, Skup M
PLoS One. 2019 Sep 26;14(9):e0222849. doi: 10.1371/journal.pone.0222849. eCollection 2019.

Cancer Research

Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues
Skalicky S, Zwiers PJ, Kuiper T, Schraml E, Hackl M, Molema G
Biotechniques. 2019 Oct 17. doi: 10.2144/btn-2019-0032.

Nanostructured Substrates for Detection and Characterization of Circulating Rare Cells: From Materials Research to Clinical Applications
Dong J, Chen JF, Smalley M, Zhao M, Ke Z, Zhu Y, Tseng HR
Adv Mater. 2019 Sep 30:e1903663. doi: 10.1002/adma.201903663.

Fatty acid beta oxidation enzyme HADHA is a novel potential therapeutic target in malignant lymphoma
Yamamoto K, Abe S, Honda A, Hashimoto J, Aizawa Y, Ishibashi S, Takemura T, Hanagata N, Yamamoto M, Miura O, Kurata M, Kitagawa M
Lab Invest. 2019 Sep 16. doi: 10.1038/s41374-019-0318-6.

Neurosciences

NCS-1 deficiency affects mRNA-levels of genes involved in regulation of ATP-Synthesis and mitochondrial stress in highly vulnerable Substantia nigra dopaminergic neurons
Liss B, Simons C, Benkert J, Deuter N, Pongs O, Schneider T, Duda J
Front. Mol. Neurosci. | doi: 10.3389/fnmol.2019.00252

Early pre- and postsynaptic decrease in glutamatergic and cholinergic signaling after spinalization is not modified when stimulating proprioceptive input to the ankle extensor α-motoneurons: Anatomical and neurochemical study
Grycz K, Głowacka A, Ji B, Czarkowska-Bauch J, Gajewska-Woźniak O, Skup M
PLoS One. 2019 Sep 26;14(9):e0222849. doi: 10.1371/journal.pone.0222849. eCollection 2019.

The median eminence as the hypothalamic area involved in rapid transfer of glucose to the brain: functional and cellular mechanisms
Martínez F, Cifuentes M, Tapia JC, Nualart F
J Mol Med (Berl). 2019 Aug;97(8):1085-1097. doi: 10.1007/s00109-019-01799-5.

Clinical Applications

Neural EGFL like 1 as a potential pro-chondrogenic, anti-inflammatory dual-functional disease-modifying osteoarthritis drug
Li C, Zheng Z, Ha P, Jiang W, Berthiaume EA, Lee S, Mills Z, Pan H, Chen EC, Jiang J, Culiat CT, Zhang X, Ting K, Soo C
Biomaterials, Volume 226, January 2020, 119541

MicroRNA23a Overexpression in Crohn's Disease Targets Tumour Necrosis Factor Alpha Inhibitor Protein 3, Increasing Sensitivity to TNF and Modifying the Epithelial Barrier
Felwick RK, Dingley GJR, Martinez-Nunez R, Sanchez-Elsner T, Cummings JRF, Collins JE
J Crohns Colitis. 2019 Oct 18. pii: jjz145. doi: 10.1093/ecco-jcc/jjz145.

Enrichment of histones from patient samples for mass spectrometry-based analysis of post-translational modifications
Noberini R, Restellini C, Oliva Savoia E, Bonaldi T
Methods. 2019 Oct 9. pii: S1046-2023(19)30136-7. doi: 10.1016/j.ymeth.2019.10.001.

Developmental Research, Epigenetics & Aging

Coordination of Neuron Production in Mouse and Human Cerebral Cortex by the Homolog of Drosophila Mastermind Protein
Ayoub AE, Dominguez MH, Benoit J, Ortega JA, Radonjic N, Zecevic N, Rakic P
Brain Behav Evol. 2019 Aug 15;93(2-3):152-165. doi: 10.1159/000500494.

Uterine spiral artery muscle dedifferentiation
Robson A, Lash GE, Innes BA, Zhang JY, Robson SC, Bulmer JN
Hum Reprod. 2019 Jul 26. pii: dez124. doi: 10.1093/humrep/dez124.

Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
Ma P, Song NN, Li Y, Zhang Q, Zhang L, Zhang L, Kong Q, Ma L, Yang X, Ren B, Li C, Zhao X, Li Y, Xu Y, Gao X, Ding YQ, Mao B
Cell Rep. 2019 Jul 9;28(2):541-553.e4. doi: 10.1016/j.celrep.2019.06.017.

Plant Research

Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Cahill JF, Kertesz V
Methods Mol Biol. 2020;2064:89-101. doi: 10.1007/978-1-4939-9831-9_7.

Tissue-Specific Metabolite Profiling on the Different Parts of Bolting and Unbolting Peucedanum praeruptorum Dunn (Qianhu) by Laser Microdissection Combined with UPLC-Q/TOF⁻MS and HPLC⁻DAD
Chen LL, Chu SS, Zhang L, Xie J, Dai M, Wu X, Peng HS
Molecules. 2019 Apr 11;24(7). pii: E1439. doi: 10.3390/molecules24071439.

Transcriptome landscape of the early Brassica napus seed
Ziegler DJ, Khan D, Kalichuk JL, Becker MG, Belmonte MF
J Integr Plant Biol. 2019 Apr 2. doi: 10.1111/jipb.12812.

Forensics

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Implication of vitality, seasonality and specific leaf area on PAH uptake inmoss and lichen transplanted in bags
Capozzi F, Sorrentino MC, Di Palma A, Mele F, Arena C, Adamo P, Spagnuolo V, Giordano S
Ecological Indicators, Volume 108, January 2020, 105727

Nanostructured Substrates for Detection and Characterization of Circulating Rare Cells: From Materials Research to Clinical Applications
Dong J, Chen JF, Smalley M, Zhao M, Ke Z, Zhu Y, Tseng HR
Adv Mater. 2019 Sep 30:e1903663. doi: 10.1002/adma.201903663.

Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Cahill JF, Kertesz V
Methods Mol Biol. 2020;2064:89-101. doi: 10.1007/978-1-4939-9831-9_7.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.