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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Topics & Tags

Table of Content

DNA Research

Proteases and their inhibitors as prognostic factors for high-grade serous ovarian cancer
Trudel D, Avarvarei L-M, Orain M, Turcotte S, Plante M, Grégoire J, Kappelhoff R, Labbé DP, Bachvarov D, Têtu B, Overall CM, Bairati I
Pathology - Research and Practice, 2 Mar 2019, doi.org/10.1016/j.prp.2019.02.019

Massively Parallel Sequencing Analysis of Benign Melanocytic Nevi
Lozada JR, Geyer FC, Selenica P, Brown D, Alemar B, Merghoub T, Berger MF, Busam KJ, Halpern AC, Weigelt B, Reis-Filho JS, Hollmann TJ
Histopathology. 2019 Feb 21. doi: 10.1111/his.13843.

dPCR Mutational Analyses in Cell-Free DNA: A Comparison with Tissues
Takeshita T, Iwase H
Methods Mol Biol. 2019;1909:105-118. doi: 10.1007/978-1-4939-8973-7_8.

RNA Research

Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles
Gorshkov O, Chernova T, Mokshina N, Gogoleva N, Suslov D, Tkachenko A, Gorshkova T
Plants (Basel). 2019 Feb 19;8(2). pii: E47. doi: 10.3390/plants8020047.

Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome
MacFarlane EG, Parker SJ, Shin JY, Ziegler SG, Creamer TJ, Bagirzadeh R, Bedja D, Chen Y, Calderon JF, Weissler K, Frischmeyer-Guerrerio PA, Lindsay ME, Habashi JP, Dietz H
J Clin Invest. 2019 Feb 1;129(2):659-675. doi: 10.1172/JCI123547. Epub 2019 Jan 7.

Thermal stress induces glycolytic beige fat formation via a myogenic state
Chen Y, Ikeda K, Yoneshiro T, Scaramozza A, Tajima K, Wang Q, Kim K, Shinoda K, Sponton CH, Brown Z, Brack A, Kajimura S
Nature. 2019 Jan;565(7738):180-185. doi: 10.1038/s41586-018-0801-z.

Proteomics

Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer
Wang Q, Yang Q, Li M, Zhang Y, Cai Y, Liang X, Fu Y, Xiao Z, Zhou M, Xie Z, Huang H, Huang Y, Chen Y, He Q, Peng F, Chen Z
bioRxiv preprint first posted online Feb. 26, 2019

Measurement of the Spatial Distribution of S1P in Small Quantities of Tissues: Development and Application of a Highly Sensitive LC-MS/MS Method Combined with Laser Microdissection
Wang J, Kano K, Saigusa D, Aoki J
Mass Spectrom (Tokyo). 2019;8(1):A0072. doi: 10.5702/massspectrometry.A0072.

An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Nagai-Okatani C, Nagai M, Sato T, Kuno A
Int J Mol Sci. 2019 Feb 6;20(3). pii: E700. doi: 10.3390/ijms20030700.

Fluorescence Applications

Role of Intraganglionic Transmission in the Trigeminovascular Pathway
Hurley JH, Kunkler PE, Zhang L, Knopp KL, Oxford GS
Mol Pain. 2019 Feb 20:1744806919836570. doi: 10.1177/1744806919836570.

Exercise-induced enhancement of synaptic function  triggered by the inverse BAR protein, Mtss1L
Chatzi C, Zhang G, Hendricks W, Chen Y, Schnell E, Goodman RH, Westbrook GL
bioRxiv, Feb. 9, 2019, dx.doi.org/10.1101/545582doi:

An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Nagai-Okatani C, Nagai M, Sato T, Kuno A
Int J Mol Sci. 2019 Feb 6;20(3). pii: E700. doi: 10.3390/ijms20030700.

Cancer Research

Proteases and their inhibitors as prognostic factors for high-grade serous ovarian cancer
Trudel D, Avarvarei L-M, Orain M, Turcotte S, Plante M, Grégoire J, Kappelhoff R, Labbé DP, Bachvarov D, Têtu B, Overall CM, Bairati I
Pathology - Research and Practice, 2 Mar 2019, doi.org/10.1016/j.prp.2019.02.019

Dihydrotestosterone inhibits arylsulfatase B and Dickkopf Wnt signaling pathway inhibitor (DKK)-3 leading to enhanced Wnt signaling in prostate epithelium in response to stromal Wnt3A
Bhattacharyya S, Feferman L, Tobacman JK
Prostate. 2019 Feb 22. doi: 10.1002/pros.23776.

Early-stage serrated adenocarcinomas are divided into several molecularly distinct subtypes
Hirano D, Urabe Y, Tanaka S, Nakamura K, Ninomiya Y, Yuge R, Hayashi R, Oka S, Kitadai Y, Shimamoto F, Arihiro K, Chayama K
PLoS One. 2019 Feb 20;14(2):e0211477. doi: 10.1371/journal.pone.0211477. eCollection 2019.

Neurosciences

Exercise-induced enhancement of synaptic function  triggered by the inverse BAR protein, Mtss1L
Chatzi C, Zhang G, Hendricks W, Chen Y, Schnell E, Goodman RH, Westbrook GL
bioRxiv, Feb. 9, 2019, dx.doi.org/10.1101/545582doi:

Neuropilin-1-mediated pruning of corticospinal tract fibers is required for motor recovery after spinal cord injury
Nakanishi T, Fujita Y, Yamashita T
Cell Death Dis. 2019 Jan 25;10(2):67. doi: 10.1038/s41419-019-1338-2.

Pathological changes of distal motor neurons after complete spinal cord injury
Yokota K, Kubota K, Kobayakawa K, Saito T, Hara M, Kijima K, Maeda T, Katoh H, Ohkawa Y, Nakashima Y, Okada S
Mol Brain. 2019 Jan 9;12(1):4. doi: 10.1186/s13041-018-0422-3.

Clinical Applications

Two types of amyloidosis presenting in a single patient: a case series
Sidiqi MH, McPhail ED, Theis JD, Dasari S, Vrana JA, Drosou ME, Leung N, Hayman S, Rajkumar SV, Warsame R, Ansell SM, Gertz MA, Grogan M, Dispenzieri A
Blood Cancer J. 2019 Mar 5;9(3):30. doi: 10.1038/s41408-019-0193-9.

Amelioration of Ductular Reaction by Stem Cell Derived Extracellular Vesicles in MDR2 knockout mice via let-7 microRNA
McDaniel K, Wu N, Zhou T, Huang L, Sato K, Venter J, Ceci L, Chen D, Ramos-Lorenzo S, Invernizzi P, Bernuzzi F, Wu C, Francis H, Glaser S, Alpini G, Meng F
Hepatology. 2019 Feb 5. doi: 10.1002/hep.30542.

Spatial and temporal changes in myogenic protein expression by the microenvironment after freeze injury
Yoon N, Chu V, Gould M, Zhang M
J Anat. 2019 Mar;234(3):359-367. doi: 10.1111/joa.12925.
doi.org/10.1111/joa.12925

Developmental Research, Epigenetics & Aging

Hypothalamic insulin receptor expression and DNA promoter methylation are sex-specifically altered in adult offspring of high-fat diet (HFD)-overfed mother rats
Schellong K, Melchior K, Ziska T, Ott R, Henrich W, Rancourt RC, Plagemann A
The Journal of Nutritional Biochemistry, 10 Feb 2019, doi.org/10.1016/j.jnutbio.2019.01.014

Thermal stress induces glycolytic beige fat formation via a myogenic state
Chen Y, Ikeda K, Yoneshiro T, Scaramozza A, Tajima K, Wang Q, Kim K, Shinoda K, Sponton CH, Brown Z, Brack A, Kajimura S
Nature. 2019 Jan;565(7738):180-185. doi: 10.1038/s41586-018-0801-z.

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Plant Research

Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles
Gorshkov O, Chernova T, Mokshina N, Gogoleva N, Suslov D, Tkachenko A, Gorshkova T
Plants (Basel). 2019 Feb 19;8(2). pii: E47. doi: 10.3390/plants8020047.

Cellular‐Specific Detection of Polyphenolic Compounds by NMR‐and MS‐Based Techniques
Hölscher D
Recent Advances in Polyphenol Research, 2019

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Forensics

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Nagai-Okatani C, Nagai M, Sato T, Kuno A
Int J Mol Sci. 2019 Feb 6;20(3). pii: E700. doi: 10.3390/ijms20030700.

Dark aerobic sulfide oxidation by anoxygenic phototrophs in anoxic waters
Berg JS, Pjevac P, Sommer T, Buckner CRT, Philippi M, Hach PF, Liebeke M, Holtappels M, Danza F, Tonolla M, Sengupta A, Schubert CJ, Milucka J, Kuypers MMM
Environ Microbiol. 2019 Jan 28. doi: 10.1111/1462-2920.14543.

Comparative localization of cystathionine beta synthases and cystathionine gamma lyase in canine, non-human primate and human retina
Badiei A, Sudharsan R, Santana E, Dunaief JL, Aguirre GD
Exp Eye Res. 2019 Jan 14;181:72-84. doi: 10.1016/j.exer.2019.01.007. [

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.