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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Table of Content

DNA Research

Molecular association of functioning stroma with carcinoma cells in the ovary: A preliminary study
Narikiyo M, Yano M, Kamada K, Katoh T, Ito K, Shuto M, Kayano H, Yasuda M
Oncol Lett. 2019 Mar;17(3):3562-3568. doi: 10.3892/ol.2019.9992.

Proteases and their inhibitors as prognostic factors for high-grade serous ovarian cancer
Trudel D, Avarvarei L-M, Orain M, Turcotte S, Plante M, Grégoire J, Kappelhoff R, Labbé DP, Bachvarov D, Têtu B, Overall CM, Bairati I
Pathology - Research and Practice, 2 Mar 2019, doi.org/10.1016/j.prp.2019.02.019

dPCR Mutational Analyses in Cell-Free DNA: A Comparison with Tissues
Takeshita T, Iwase H
Methods Mol Biol. 2019;1909:105-118. doi: 10.1007/978-1-4939-8973-7_8.

RNA Research

Polyploid Hepatocytes Facilitate Adaptation and Regeneration to Chronic Liver Injury
Wilkinson PD, Alencastro F, Delgado ER, Leek MP, Weirich MP, Otero PA, Roy N, Brown WK, Oertel M, Duncan AW
Am J Pathol. 2019 Mar 27. pii: S0002-9440(18)30970-2. doi: 10.1016/j.ajpath.2019.02.008.

High-throughput RNA sequencing from paired lesional- and non-lesional skin reveals major alterations in the psoriasis circRNAome
Moldovan L-I, Birballe Hansen T, Trillingsgaard Venø M, Okholm TLH, Andersen TL, Hager H, Iversen L, Kjems J, Johansen C Kristensen LS
bioRxiv, 581066; doi: doi.org/10.1101/581066

Profibrotic epithelial phenotype: a central role for MRTF and TAZ
Bialik JF, Ding M, Speight P, Dan Q, Miranda MZ, Di Ciano-Oliveira C, Kofler MM, Rotstein OD, Pedersen SF, Szászi K, Kapus A
Sci Rep. 2019 Mar 13;9(1):4323. doi: 10.1038/s41598-019-40764-7.

Proteomics

Quantitative proteomic profiling of tumor-associated vascular endothelial cells in colorectal cancer
Wang Q, Yang Q, Li M, Zhang Y, Cai Y, Liang X, Fu Y, Xiao Z, Zhou M, Xie Z, Huang H, Huang Y, Chen Y, He Q, Peng F, Chen Z
bioRxiv preprint first posted online Feb. 26, 2019

Measurement of the Spatial Distribution of S1P in Small Quantities of Tissues: Development and Application of a Highly Sensitive LC-MS/MS Method Combined with Laser Microdissection
Wang J, Kano K, Saigusa D, Aoki J
Mass Spectrom (Tokyo). 2019;8(1):A0072. doi: 10.5702/massspectrometry.A0072.

An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Nagai-Okatani C, Nagai M, Sato T, Kuno A
Int J Mol Sci. 2019 Feb 6;20(3). pii: E700. doi: 10.3390/ijms20030700.

Fluorescence Applications

Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Swieck K, Conta-Steencken A, Middleton FA, Siebert JR, Osterhout DJ, Stelzner DJ
BMC Neurosci. 2019 Mar 18;20(1):10. doi: 10.1186/s12868-019-0491-y.

Pinealectomy or light exposure exacerbates biliary damage and liver fibrosis in cholestatic rats through decreased melatonin synthesis
Chen L, Zhou T, Wu N, O’Brien A, Venter J, Ceci L, Kyritsi K, Onori P, Gaudio E, Sybenga A, Xie L, Wu C, Fabris L, Invernizzi P, Zawieja D, Liangpunsakul S, Meng F, Francis H, Glaser S
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 16 Mar 2019, doi.org/10.1016/j.bbadis.2019.03.002

Role of Intraganglionic Transmission in the Trigeminovascular Pathway
Hurley JH, Kunkler PE, Zhang L, Knopp KL, Oxford GS
Mol Pain. 2019 Feb 20:1744806919836570. doi: 10.1177/1744806919836570.

Cancer Research

Investigation of the clonal origin of multifocal papillary thyroid carcinoma according to the X‑chromosome inactivation pattern
Chen D, Qi W, Feng L, Wang J, Wang L, Guan H
Oncology Letters 17, no. 5 (2019): 4695-4700. doi.org/10.3892/ol.2019.10105

Molecular association of functioning stroma with carcinoma cells in the ovary: A preliminary study
Narikiyo M, Yano M, Kamada K, Katoh T, Ito K, Shuto M, Kayano H, Yasuda M
Oncol Lett. 2019 Mar;17(3):3562-3568. doi: 10.3892/ol.2019.9992.

Proteases and their inhibitors as prognostic factors for high-grade serous ovarian cancer
Trudel D, Avarvarei L-M, Orain M, Turcotte S, Plante M, Grégoire J, Kappelhoff R, Labbé DP, Bachvarov D, Têtu B, Overall CM, Bairati I
Pathology - Research and Practice, 2 Mar 2019, doi.org/10.1016/j.prp.2019.02.019

Neurosciences

Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Swieck K, Conta-Steencken A, Middleton FA, Siebert JR, Osterhout DJ, Stelzner DJ
BMC Neurosci. 2019 Mar 18;20(1):10. doi: 10.1186/s12868-019-0491-y.

Exercise-induced enhancement of synaptic function  triggered by the inverse BAR protein, Mtss1L
Chatzi C, Zhang G, Hendricks W, Chen Y, Schnell E, Goodman RH, Westbrook GL
bioRxiv, Feb. 9, 2019, dx.doi.org/10.1101/545582doi:

Neuropilin-1-mediated pruning of corticospinal tract fibers is required for motor recovery after spinal cord injury
Nakanishi T, Fujita Y, Yamashita T
Cell Death Dis. 2019 Jan 25;10(2):67. doi: 10.1038/s41419-019-1338-2.

Clinical Applications

Polyploid Hepatocytes Facilitate Adaptation and Regeneration to Chronic Liver Injury
Wilkinson PD, Alencastro F, Delgado ER, Leek MP, Weirich MP, Otero PA, Roy N, Brown WK, Oertel M, Duncan AW
Am J Pathol. 2019 Mar 27. pii: S0002-9440(18)30970-2. doi: 10.1016/j.ajpath.2019.02.008.

Profibrotic epithelial phenotype: a central role for MRTF and TAZ
Bialik JF, Ding M, Speight P, Dan Q, Miranda MZ, Di Ciano-Oliveira C, Kofler MM, Rotstein OD, Pedersen SF, Szászi K, Kapus A
Sci Rep. 2019 Mar 13;9(1):4323. doi: 10.1038/s41598-019-40764-7.

Two types of amyloidosis presenting in a single patient: a case series
Sidiqi MH, McPhail ED, Theis JD, Dasari S, Vrana JA, Drosou ME, Leung N, Hayman S, Rajkumar SV, Warsame R, Ansell SM, Gertz MA, Grogan M, Dispenzieri A
Blood Cancer J. 2019 Mar 5;9(3):30. doi: 10.1038/s41408-019-0193-9.

Developmental Research, Epigenetics & Aging

Hypothalamic insulin receptor expression and DNA promoter methylation are sex-specifically altered in adult offspring of high-fat diet (HFD)-overfed mother rats
Schellong K, Melchior K, Ziska T, Ott R, Henrich W, Rancourt RC, Plagemann A
The Journal of Nutritional Biochemistry, 10 Feb 2019, doi.org/10.1016/j.jnutbio.2019.01.014

Thermal stress induces glycolytic beige fat formation via a myogenic state
Chen Y, Ikeda K, Yoneshiro T, Scaramozza A, Tajima K, Wang Q, Kim K, Shinoda K, Sponton CH, Brown Z, Brack A, Kajimura S
Nature. 2019 Jan;565(7738):180-185. doi: 10.1038/s41586-018-0801-z.

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Plant Research

Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles
Gorshkov O, Chernova T, Mokshina N, Gogoleva N, Suslov D, Tkachenko A, Gorshkova T
Plants (Basel). 2019 Feb 19;8(2). pii: E47. doi: 10.3390/plants8020047.

Cellular‐Specific Detection of Polyphenolic Compounds by NMR‐and MS‐Based Techniques
Hölscher D
Recent Advances in Polyphenol Research, 2019

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Forensics

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Relationship between Mg, B and Mn status and tomato tolerance against Cd toxicity
Carvalho MEA, Piotto FA, Franco MR, Rossi ML, Martinelli AP, Cuypers A, Azevedo RA
J Environ Manage. 2019 Mar 28;240:84-92. doi: 10.1016/j.jenvman.2019.03.026.

Mitochondria and oxidative stress in ovarian endometriosis
Chen C, Zhou Y, Hu C, Wang Y, Yan Z, Li Z, Wu R
Free Radic Biol Med. 2019 Mar 26. pii: S0891-5849(19)30264-3. doi: 10.1016/j.freeradbiomed.2019.03.027.

Enhanced spinal cord microstimulation using conducting polymer-coated carbon microfibers
Vara H, Collazos-Castro JE
Acta Biomater. 2019 Mar 21. pii: S1742-7061(19)30212-0. doi: 10.1016/j.actbio.2019.03.037.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.