Story

Laser Microdissection Publication List

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

Authors

Topics & Tags

DNA Research

HJ, Cha IH, Cha YH:
Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas
PLoS One. 2021 Sep 3;16(9):e0256979. doi: 10.1371/journal.pone.0256979. eCollection 2021.

Kobayashi H, Nakai T, Nakanishi Y, Esumi M, Masuda S:
Phylogenetic analysis of combined lobular and ductal carcinoma of the breast
Mol Med Rep. 2021 Oct;24(4):718. doi: 10.3892/mmr.2021.12357. 

Winick-Ng W, Kukalev A, Harabula I, Redondo LZ, Szabo D, Meijer M, Serebreni L, Zhang Y, Bianco S, Chiariello AM, Irastorza-Azcarate I, Thieme CJ, Sparks TM, Carvalho S, Fiorillo L, Musella F, Irani E, Triglia ET, Kolodziejczyk AA, Abentung A, Apostolova A, Paul EJ, Franke V, Kempfer R, Akalin A, Teichmann SA, Dechant G, Ungless MA, Nicodemi M, Welch L, Castelo-Branco G, Pombo A:
Cell-type specialization in the brain is encoded by specific long-range chromatin topologies
bioRxiv, 2021; doi: doi.org/10.1101/2020.04.02.020990

RNA Research

Tjaden J, Eickhoff A, Stahlke S, Gehmeyr J, Vorgerd M, Theis V , Matschke V and Theiss C:
Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment
Cells 2021, 10(9), 2277; doi.org/10.3390/cells10092277

Kolega J, Poppenberg KE, Lim HW, Gutierrez LC, Veeturi SS, Siddiqui AH, Rajabzadeh-Oghaz H, Tutino VM: 
Identification of intima-to-media signals for flow-induced vascular remodeling using correlative gene expression analysis
Sci Rep. 2021 Aug 9;11(1):16142. doi: 10.1038/s41598-021-95403-x.

Matsuyama S, Pervin M, Nakagawa M, Izawa T, Kuwamura M, Yamate J:
Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin
J Vet Med Sci. 2021 Jul 22. doi: 10.1292/jvms.21-0341.

Proteomics, Lipidomics, Metabolomics

Robertson GT, Ramey ME, Massoudi LM, Carter CL, Zimmerman M, Kaya F, Graham BG, Gruppo V, Hastings C, Woolhiser LK, Scott DWL, Asay BC, Eshun-Wilson F, Maidj E, Podell BK, Vásquez JJ, Lyons MA, Dartois V, Lenaerts AJ:
Comparative Analysis of Pharmacodynamics in the C3HeB/FeJ Mouse Tuberculosis Model for DprE1 inhibitors TBA-7371, PBTZ169 and OPC-167832
Antimicrob Agents Chemother. 2021 Aug 9:AAC0058321. doi: 10.1128/AAC.00583-21.

Noberini R, Savoia EO, Brandini S, Greco F, Marra F, Bertalot G, Pruneri G, McDonnell LA, Bonaldi T:
Spatial epi-proteomics enabled by histone post-translational modification analysis from low-abundance clinical samples
Clin Epigenetics. 2021 Jul 28;13(1):145. doi: 10.1186/s13148-021-01120-7.

Ernest JP, Sarathy J, Wang N, Kaya F, Zimmerman MD, Strydom N, Wang H, Xie M, Gengenbacher M, Via LE, Barry CE 3rd, Carter CL, Savic RM, Dartois V:
Lesion penetration and activity limit the utility of second-line injectable agents in pulmonary tuberculosis.
Antimicrob Agents Chemother. 2021 Jul 12:AAC0050621. doi: 10.1128/AAC.00506-21.

Fluorescence Applications

Zhao Y, Chu S, Gui S, Qin Y, Xu R, Shan T, Peng H:
Tissue-specific metabolite profiling of Fallopia multiflora (Heshouwu) and Fallopia multiflora var. angulata by mass spectrometry imaging and laser microdissection combined with UPLC-Q/TOF-MS
Journal of Pharmaceutical and Biomedical Analysis, Volume 200, 5 June 2021, 114070

Winick-Ng W, Kukalev A, Harabula I, Redondo LZ, Szabo D, Meijer M, Serebreni L, Zhang Y, Bianco S, Chiariello AM, Irastorza-Azcarate I, Thieme CJ, Sparks TM, Carvalho S, Fiorillo L, Musella F, Irani E, Triglia ET, Kolodziejczyk AA, Abentung A, Apostolova A, Paul EJ, Franke V, Kempfer R, Akalin A, Teichmann SA, Dechant G, Ungless MA, Nicodemi M, Welch L, Castelo-Branco G, Pombo A:
Cell-type specialization in the brain is encoded by specific long-range chromatin topologies
bioRxiv, 2021; doi: doi.org/10.1101/2020.04.02.020990

Li D, Liu J, Yang C, Tian Y, Yin C, Hu L, Chen Z, Zhao F, Zhang R, Lu A, Zhang G, Qian A:
Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging
Theranostics. 2021 Mar 20;11(11):5585-5604. doi: 10.7150/thno.54477.

Cancer Research

Chen ZH, Yan SM, Chen XX, Zhang Q, Liu SX, Liu Y, Luo YL, Zhang C, Xu M, Zhao YF, Huang LY, Liu BL, Xia TL, Xu DZ, Liang Y, Chen YM, Wang W, Yuan SQ, Zhang HZ, Yun JP, Zhai WW, Zeng MS, Bai F, Zhong Q :
The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma.
Genome Med. 2021 Sep 7;13(1):146. doi: 10.1186/s13073-021-00963-2.

Kobayashi H, Nakai T, Nakanishi Y, Esumi M, Masuda S:
Phylogenetic analysis of combined lobular and ductal carcinoma of the breast
Mol Med Rep. 2021 Oct;24(4):718. doi: 10.3892/mmr.2021.12357.

Cui Y, Kim HS, Cho ES, Han D, Park JA, Park JY, Nam W, Kim HJ, Cha IH, Cha YH:
Longitudinal detection of somatic mutations in saliva and plasma for the surveillance of oral squamous cell carcinomas
PLoS One. 2021 Sep 3;16(9):e0256979. doi: 10.1371/journal.pone.0256979. eCollection 2021.

Neurosciences

Tjaden J, Eickhoff A, Stahlke S, Gehmeyr J, Vorgerd M, Theis V , Matschke V and Theiss C:
Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment
Cells 2021, 10(9), 2277; doi.org/10.3390/cells10092277

Malik S, Valdebenito S, D'Amico D, Prideaux B, Eugenin EA:
HIV infection of astrocytes compromises inter-organelle interactions and inositol phosphate metabolism: a potential mechanism of bystander damage and viral reservoir survival
Prog Neurobiol. 2021 Aug 26:102157. doi: 10.1016/j.pneurobio.2021.102157.

Winick-Ng W, Kukalev A, Harabula I, Redondo LZ, Szabo D, Meijer M, Serebreni L, Zhang Y, Bianco S, Chiariello AM, Irastorza-Azcarate I, Thieme CJ, Sparks TM, Carvalho S, Fiorillo L, Musella F, Irani E, Triglia ET, Kolodziejczyk AA, Abentung A, Apostolova A, Paul EJ, Franke V, Kempfer R, Akalin A, Teichmann SA, Dechant G, Ungless MA, Nicodemi M, Welch L, Castelo-Branco G, Pombo A:
Cell-type specialization in the brain is encoded by specific long-range chromatin topologies
bioRxiv, 2021; doi: doi.org/10.1101/2020.04.02.020990

Clinical/Pathology/Immunology Research Applications

Malik S, Valdebenito S, D'Amico D, Prideaux B, Eugenin EA:
HIV infection of astrocytes compromises inter-organelle interactions and inositol phosphate metabolism: a potential mechanism of bystander damage and viral reservoir survival
Prog Neurobiol. 2021 Aug 26:102157. doi: 10.1016/j.pneurobio.2021.102157.

Robertson GT, Ramey ME, Massoudi LM, Carter CL, Zimmerman M, Kaya F, Graham BG, Gruppo V, Hastings C, Woolhiser LK, Scott DWL, Asay BC, Eshun-Wilson F, Maidj E, Podell BK, Vásquez JJ, Lyons MA, Dartois V, Lenaerts AJ:
Comparative Analysis of Pharmacodynamics in the C3HeB/FeJ Mouse Tuberculosis Model for DprE1 inhibitors TBA-7371, PBTZ169 and OPC-167832
Antimicrob Agents Chemother. 2021 Aug 9:AAC0058321. doi: 10.1128/AAC.00583-21.

Matsuyama S, Pervin M, Nakagawa M, Izawa T, Kuwamura M, Yamate J:
Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin
J Vet Med Sci. 2021 Jul 22. doi: 10.1292/jvms.21-0341.

Developmental Research, Epigenetics & Aging

Miyai K, Yonekura Y, Ito K, Matsukuma S, Tsuda H :
Gene expression microarray analysis of adult testicular germ cell tumor: a comparison between pure-type seminomas and seminoma components in mixed tumors
Virchows Arch. 2021 Aug 4. doi: 10.1007/s00428-021-03168-5.

Noberini R, Savoia EO, Brandini S, Greco F, Marra F, Bertalot G, Pruneri G, McDonnell LA, Bonaldi T:
Spatial epi-proteomics enabled by histone post-translational modification analysis from low-abundance clinical samples
Clin Epigenetics. 2021 Jul 28;13(1):145. doi: 10.1186/s13148-021-01120-7.

Wang D,  Guo C, Li Y, Zhou M, Wang H, Liu J, Chen P:
Estrogen upregulates DNMT1 and leads to the hypermethylation of RUNX3 inthe malignant transformation of ovarian endometriosis
Reproductive BioMedicine Online(2021), doi:https://doi.org/10.1016/j.rbmo.2021.06.030

Plant Research

Ishimaru T, Parween S, Saito Y, Masamura T, Kondo M, Sreenivasutu N:
Laser Microdissection Transcriptome Data Derived Gene Regulatory Networks of Developing Rice Endosperm Revealed Tissue- and Stage-specific Regulators Modulating Starch Metabolism
Research Square, 23 Aug 2021, DOI: 10.21203/rs.3.rs-814515/v1

Jia HH, Xu YT, Yin ZP, Wu XM, Qing M, Fan YJ, Song X, Xie KD, Xie ZZ, Xu Q, Deng XX, Guo WW:
Transcriptomes and DNA methylomes in apomictic cells delineate nucellar embryogenesis initiation in citrus
DNA Res. 2021 Aug 23:dsab014. doi: 10.1093/dnares/dsab014.

Jhu M-Y, Ichihashi Y, Farhi M, Wong C, Sinha NR:
LATERAL ORGAN BOUNDARIES DOMAIN 25 functions as a key regulator of haustorium development in dodders
Plant Physiology, 2021 May 27, kiab231, doi.org/10.1093/plphys/kiab231

Forensics

Prajapati S, Rajmane P, Jayakrishna P, Nair MS, Kshirsagar P and Meshram M:
Application and utility of alternative methods in isolation of pure cells from forensic biological mixtures in modern-day: a review
JFSR – Journal of Forensic Science and Research, Aug 2021, ISSN 2575-0186

Chong KWY, Thong Z, Kiu-Choong Syn C :
Recent trends and developments in forensic DNA extraction
WIREs Forensic Science, 30 July 2020, DOI: 10.1002/wfs2.1395

England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison SA:
Compatibility of the ForenSeq™ DNA Signature Prep Kit with laser microdissected cells: An exploration of issues that arise with samples containing low cell numbers
Forensic Science International: Genetics, 1 May 2020, 102278

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Foster RA, Tienken D, Littmann S, Whitehouse MJ, Kuypers MMM, White AE:
The rate and fate of N2 and C fixation by marine diatom-diazotroph symbioses ISME J. 2021 Aug 24. doi: 10.1038/s41396-021-01086-7.

Duerschlag J, Mohr W, Ferdelman TG, LaRoche J, Desai D, Croot PL, Voß D, Zielinski O, Lavik G, Littmann S, Martínez-Pérez C, Tschitschko B, Bartlau N, Osterholz H, Dittmar T, Kuypers MMM:
Niche partitioning by photosynthetic plankton as a driver of CO2-fixation across the oligotrophic South Pacific Subtropical Ocean
ISME J. 2021 Aug 19. doi: 10.1038/s41396-021-01072-z.

Lentini G, Ben Chaabene R, Vadas O, Ramakrishnan C, Mukherjee B, Mehta V, Lunghi M, Grossmann J, Maco B, Visentin R, Hehl AB, Korkhov VM, Soldati-Favre D:
Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion.
Nat Commun. 2021 Jun 18;12(1):3788. doi: 10.1038/s41467-021-24083-y.

Live Cell Culture

Malik S, Valdebenito S, D'Amico D, Prideaux B, Eugenin EA:
HIV infection of astrocytes compromises inter-organelle interactions and inositol phosphate metabolism: a potential mechanism of bystander damage and viral reservoir survival
Prog Neurobiol. 2021 Aug 26:102157. doi: 10.1016/j.pneurobio.2021.102157.

Valdebenito S, Malik S, Luu R, Loudig O, Mitchell M, Okafo G, Bhat K, Prideaux B, Eugenin EA:
Tunneling nanotubes, TNT, communicate glioblastoma with surrounding non-tumor astrocytes to adapt them to hypoxic and metabolic tumor conditions
Sci Rep. 2021 Jul 15;11(1):14556. doi: 10.1038/s41598-021-93775-8.

Pensold D, Zimmer-Bensch G:
Methods for Single-Cell Isolation and Preparation
In: Yu B., Zhang J., Zeng Y., Li L., Wang X. (eds) Single-cell Sequencing and Methylation. Advances in Experimental Medicine and Biology, vol 1255.

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?

  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009

You will find a more detailed list of local contacts here.