Contact & Support

Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

Authors

Topics & Tags

Table of Content

DNA Research

Tipifarnib Inhibits HRAS-Driven Dedifferentiated Thyroid Cancers
Untch BR, Dos Anjos V, Garcia-Rendueles MER, Knauf JA, Krishnamoorthy GP, Saqcena M, Bhanot UK, Socci ND, Ho AL, Ghossein R, Fagin JA
Cancer Res. 2018 Aug 15;78(16):4642-4657. doi: 10.1158/0008-5472.CAN-17-1925.

Precancerous neoplastic cells can move through the pancreatic ductal system
Makohon-Moore AP, Matsukuma K, Zhang M, Reiter JG, Gerold JM, Jiao Y, Sikkema L, Attiyeh MA, Yachida S, Sandone C, Hruban RH, Klimstra DS, Papadopoulos N, Nowak MA, Kinzler KW, Vogelstein B, Iacobuzio-Donahue CA
Nature. 2018 Sep;561(7722):201-205. doi: 10.1038

Difference in transducin-like enhancer of split 1 protein expression between basal cell adenomas and basal cell adenocarcinomas - an immunohistochemical study
Oyama Y, Nishida H, Kusaba T, Kadowaki H, Arakane M, Wada J, Urabe S, Hirano T, Kawano K, Suzuki M, Yokoyama S, Daa T
Diagn Pathol. 2018 Jul 27;13(1):48. doi: 10.1186/s13000-018-0726-8.

RNA Research

Temporal Regulation of Glomerular and Cortical Tubulointerstitial Genes Involved in the Development of Nephrotoxic Serum Nephritis
Ougaard ME, Sembach FE, Kvist PH, Tonnesen M, Frederiksen KS, Egfjord M, Jensen HE, Galsgaard E
Nephron. 2018;140(3):218-230. doi: 10.1159/000492294.

Prometastatic Molecular Profiles in Breast Tumors From Socially Isolated Women
Bower JE, Shiao SL, Sullivan P, Lamkin DM, Atienza R, Mercado F, Arevalo J, Asher A, Ganz PA, Cole SW
JNCI Cancer Spectr. 2018 Jul;2(3):pky029. doi: 10.1093/jncics/pky029. Epub 2018 Jul 19.

Knockout of secretin receptor reduces biliary damage and liver fibrosis in Mdr2-/- mice by diminishing senescence of cholangiocytes
Zhou T, Wu N, Meng F, Venter J, Giang TK, Francis H, Kyritsi K, Wu C, Franchitto A, Alvaro D, Marzioni M, Onori P, Mancinelli R, Gaudio E, Glaser S, Alpini G
Lab Invest. 2018 Jul 5. doi: 10.1038/s41374-018-0093-9.

Proteomics

Differential Proteomic Analysis between Small Cell Lung Carcinoma (SCLC) and Pulmonary Carcinoid Tumors Reveals Molecular Signatures for Malignancy in Lung Cancer
Fujii K, Miyata Y, Takahashi I, Koizumi H, Saji H, Hoshikawa M, Takagi M, Nishimura T, Nakamura
Proteomics Clin Appl. 2018 Nov;12(6):e1800015. doi: 10.1002/prca.201800015.

Lactic acid induces lactate transport and glycolysis/OXPHOS interconversion in glioblastoma

Duan K, Liu ZJ, Hu SQ, Huo HY, Xu ZR, Ruan JF, Sun Y, Dai LP, Yan CB, Xiong W, Cui QH, Yu HJ, Yu M, Qin Y
Biochem Biophys Res Commun. 2018 Jun 20. pii: S0006-291X(18)31404-9. doi: 10.1016/j.bbrc.2018.06.092.

Proteomics analysis identifies new markers associated with capillary cerebral amyloid angiopathy in Alzheimer's disease
Hondius DC, Eigenhuis KN, Morrema THJ, van der Schors RC, van Nierop P, Bugiani M, Li KW, Hoozemans JJM, Smit AB, Rozemuller AJM
Acta Neuropathol Commun. 2018 Jun 4;6(1):46. doi: 10.1186/s40478-018-0540-2.

Fluorescence Applications

Effects of nerve growth factor neutralization on TRP channel expression in laser-captured bladder afferent neurons in mice with spinal cord injury
Shimizu N, Wada N, Shimizu T, Suzuki T, Takaoka EI, Kanai AJ, de Groat WC, Hirayama A, Hashimito M, Uemura H, Yoshimura N
Neurosci Lett. 2018 Jun 27. pii: S0304-3940(18)30452-X. doi: 10.1016/j.neulet.2018.06.049.

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix
Liang L, Xu J, Liang ZT, Dong XP, Chen HB, Zhao ZZ
Molecules. 2018 May 8;23(5). pii: E1115. doi: 10.3390/molecules23051115.

Upregulation of HDAC2 in Laser Capture Nigral Microglia in Parkinson’s Disease
Tan Y, Delvaux E, Nolz J, Coleman PD, Chen S, Mastroeni D
Neurobiology of Aging, 2018, ISSN 0197-4580, doi.org/10.1016/j.neurobiolaging.2018.02.018.

Cancer Research

Sensitive tumour detection and classification using plasma cell-free DNA methylomes
Shen SY, Singhania R, Fehringer G, Chakravarthy A, Roehrl MHA, Chadwick D, Zuzarte PC, Borgida A, Wang TT, Li T, Kis O, Zhao Z, Spreafico A, Medina TDS, Wang Y, Roulois D, Ettayebi I, Chen Z, Chow S, Murphy T, Arruda A, O'Kane GM, Liu J, Mansour M, McPherson JD, O'Brien C, Leighl N, Bedard PL, Fleshner N, Liu G, Minden MD, Gallinger S, Goldenberg A, Pugh TJ, Hoffman MM, Bratman SV, Hung RJ, De Carvalho DD
Nature. 2018 Nov 14. doi: 10.1038/s41586-018-0703-0.

Precancerous neoplastic cells can move through the pancreatic ductal system
Makohon-Moore AP, Matsukuma K, Zhang M, Reiter JG, Gerold JM, Jiao Y, Sikkema L, Attiyeh MA, Yachida S, Sandone C, Hruban RH, Klimstra DS, Papadopoulos N, Nowak MA, Kinzler KW, Vogelstein B, Iacobuzio-Donahue CA
Nature. 2018 Sep;561(7722):201-205. doi: 10.1038

Tipifarnib Inhibits HRAS-Driven Dedifferentiated Thyroid Cancers
Untch BR, Dos Anjos V, Garcia-Rendueles MER, Knauf JA, Krishnamoorthy GP, Saqcena M, Bhanot UK, Socci ND, Ho AL, Ghossein R, Fagin JA
Cancer Res. 2018 Aug 15;78(16):4642-4657. doi: 10.1158/0008-5472.CAN-17-1925.

Neurosciences

Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees
Khan AM, Grant AH, Martinez A, Burns GAPC, Thatcher BS, Anekonda VT, Thompson BW, Roberts ZS, Moralejo DH, Blevins JE
Adv Neurobiol. 2018;21:101-193. doi: 10.1007/978-3-319-94593-4_6.

Purkinje cell COX deficiency and mtDNA depletion in an animal model of spinocerebellar ataxia type 1
Ripolone M, Lucchini V, Ronchi D, Fagiolari G, Bordoni A, Fortunato F, Mondello S, Bonato S, Meregalli M, Torrente Y, Corti S, Comi GP, Moggio M, Sciacco M
J Neurosci Res. 2018 Sep;96(9):1576-1585. doi: 10.1002/jnr.24263.

Effects of nerve growth factor neutralization on TRP channel expression in laser-captured bladder afferent neurons in mice with spinal cord injury
Shimizu N, Wada N, Shimizu T, Suzuki T, Takaoka EI, Kanai AJ, de Groat WC, Hirayama A, Hashimito M, Uemura H, Yoshimura N
Neurosci Lett. 2018 Jun 27. pii: S0304-3940(18)30452-X. doi: 10.1016/j.neulet.2018.06.049.
linkinghub.elsevier.com/retrieve/pii/S0304-3940(18)30452-X

Clinical Applications

The Secretin/Secretin Receptor Axis Modulates Ductular Reaction and Liver Fibrosis through Changes in Transforming Growth Factor-β1-Mediated Biliary Senescence
Wu N, Meng F, Zhou T, Venter J, Giang TK, Kyritsi K, Wu C, Alvaro D, Onori P, Mancinelli R, Gaudio E, Francis H, Alpini G, Glaser S, Franchitto A
Am J Pathol. 2018 Jul 21. pii: S0002-9440(18)30150-0. doi: 10.1016/j.ajpath.2018.06.015.

Effects of BNN27, a novel C17-spiroepoxy steroid derivative, on experimental retinal detachment-induced photoreceptor cell death
Tsoka P, Matsumoto H, Maidana DE, Kataoka K, Naoumidi I, Gravanis A, Vavvas DG, Tsilimbaris MK
Sci Rep. 2018 Jul 13;8(1):10661. doi: 10.1038/s41598-018-28633-1.

miR-103 promotes endothelial maladaptation by targeting lncWDR59
Natarelli L, Geißler C, Csaba G, Wei Y, Zhu M, di Francesco A, Hartmann P, Zimmer R, Schober A
Nat Commun. 2018 Jul 6;9(1):2645. doi: 10.1038/s41467-018-05065-z.

Developmental Research, Epigenetics & Aging

Seminal plasma modulates the immune-cytokine network in the porcine uterine tissue and pre-ovulatory follicles
Waberski D, Schäfer J, Bölling A, Scheld M, Henning H, Hambruch N, Schuberth HJ, Pfarrer C, Wrenzycki C, Hunter RH
PLoS One. 2018 Aug 28;13(8):e0202654. doi: 10.1371/journal.pone.0202654. eCollection 2018.

Runx1-Stat3 signaling regulates the epithelial stem cells in continuously growing incisors
Sarper SE, Inubushi T, Kurosaka H, Ono Minagi H, Kuremoto KI, Sakai T, Taniuchi I, Yamashiro T
Sci Rep. 2018 Jul 19;8(1):10906. doi: 10.1038/s41598-018-29317-6

Four-dimensional, dynamic mosaicism is a hallmark of normal human skin that permits mapping of the organization and patterning of human epidermis during terminal differentiation
Wang Y, Masaki T, Khan SG, Tamura D, Kuschal C, Rogers M, DiGiovanna JJ, Kraemer KH
PLoS One. 2018 Jun 13;13(6):e0198011. doi: 10.1371/journal.pone.0198011. eCollection 2018.

Plant Research

Auxin analysis using laser microdissected plant tissues sections
Muñoz-Sanhueza LG, Lee Y, Tillmann M, Cohen JD, Hvoslef-Eide AK
BMC Plant Biol. 2018 Jun 25;18(1):133. doi: 10.1186/s12870-018-1352-z.

Tissue-specific chemical profiling and quantitative analysis of bioactive components of Cinnamomum cassia by combining laser-microdissection with UPLC-Q/TOF-MS
Zhou W, Liang Z, Li P, Zhao Z, Chen J
Chem Cent J. 2018 Jun 21;12(1):71. doi: 10.1186/s13065-018-0438-x.

Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix
Liang L, Xu J, Liang ZT, Dong XP, Chen HB, Zhao ZZ
Molecules. 2018 May 8;23(5). pii: E1115. doi: 10.3390/molecules23051115.

Forensics

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017

Forensic Biology – Expert Evidence
Gunn P, Roebuck H, Summerell A
Bookchapter, reviewed March 2017

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Cell invasion and pyruvate oxidase derived H2O2 are critical for Streptococcus pneumoniae mediated cardiomyocyte killing
Brissac T, Shenoy AT, Patterson LA, Orihuela CJ
Infect Immun. 2017 Oct 23. pii: IAI.00569-17. doi: 10.1128/IAI.00569-17.

A low-noise silicon nitride nanopore device on a polymer substrate
Choi W, Jeon ES, Chun KY, Kim YR, Park KB, Kim KB, Han CS
PLoS One. 2018 Jul 20;13(7):e0200831. doi: 10.1371/journal.pone.0200831. eCollection 2018.

Hard and soft plastic resin embedding for single-cell element uptake investigations of marine-snow-associated microorganisms using nano-scale secondary ion mass spectrometry
Rogge A, Flintrop CM, Iversen MH, Salter I, Fong AA, Vogts A, Waite AM
Limnol. Oceanogr.: Methods 00, 2018, 00–00

Live Cell Culture

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.