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Laser Microdissection Publication List

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

Authors

Topics & Tags

Table of Content

DNA Research

Somatic evolution in non-neoplastic IBD-affected colon
Olafsson S, McIntyre RE, Coorens T, Butler T, Jung H, Robinson P, Lee-Six H, Sanders MA, Arestang K, Dawson C, Tripathi M, Strongili K, Hooks Y, Stratton MR, Parkes M, Martincorena I, Raine T, Campbell PJ, Anderson CA
bioRxiv, 12 Mar 2020, doi: doi.org/10.1101/832014

Intraductal papillary neoplasms of the bile duct are consisted of two distinct types specifically associated with clinicopathological features and molecular phenotypes
Aoki Y, Mizuma M, Hata T, Aoki T, Omori Y, Ono Y, Mizukami Y, Unno M, Furukawa T
J Pathol. 2020 Feb 26. doi: 10.1002/path.5398.

Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis
Chen M, Bui AQ, Goldberg RB
Methods Mol Biol. 2020;2122:205-222. doi: 10.1007/978-1-0716-0342-0_15.

RNA Research

Tsukushi is essential for the development of the inner ear
Miwa T, Ohta K, Ito N, Hattori S, Miyakawa T, Takeo T, Nakagata N, Song WJ, Minoda R
Mol Brain. 2020 Mar 3;13(1):29. doi: 10.1186/s13041-020-00570-z.

Synaptotagmin 13 is neuroprotective across motor neuron diseases
Nizzardo M, Taiana M, Rizzo F, Aguila Benitez J, Nijssen J, Allodi I, Melzi V, Bresolin N, Comi GP, Hedlund E, Corti S
Acta Neuropathol. 2020 Feb 17. doi: 10.1007/s00401-020-02133-x.

Laser Capture Micro-dissection (LCM) of Neonatal Mouse Forebrain for RNA Isolation
Roy A, Deng M, Aldinger KA, Glass IA, Millen KJ
Bio-protocol, Vol 10, Iss 1, January 05, 2020; DOI:   10.21769/BioProtoc.3475

Proteomics, Lipidomics, Metabolomics

The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow
Hunt AL, Pierobon M, Baldelli E, Oliver J, Mitchell D, Gist G, Bateman NW, Larry Maxwell G, Petricoin EF, Conrads TP
Clin Proteomics. 2020 Mar 9;17:9. doi: 10.1186/s12014-020-09272-z. eCollection 2020.

Proteomics Reveals Extracellular Matrix Injury in the Glomeruli and Tubulointerstitium of Kidney Allografts with Early Antibody-Mediated Rejection
Clotet-Freixas S, McEvoy CM, Batruch I, Van J, Pastrello C, Kotlyar M, Arambewela M, Boshart A, Farkona S, Niu Y, Li Y, Famure O, Bozovic A, Kulasingam V, Chen P, Kim J, Chan E, Moshkelgosha S, Martinu T, Juvet S, Jurisica I, Chruscinski A, John R, Konvalinka A
bioRxiv 2020.03.03.975672; doi: doi.org/10.1101/2020.03.03.975672

Tissue distribution of doxycycline in animal models of tuberculosis
Gengenbacher M, Zimmerman MD, Sarathy JP, Kaya F, Wang H, Mina M, Carter C, Hossen MA, Su H, Trujillo C, Ehrt S, Schnappinger D, Dartois V
Antimicrob Agents Chemother. 2020 Feb 10. pii: AAC.02479-19. doi: 10.1128/AAC.02479-19.

Fluorescence Applications

Dynamically Monitoring the Clonal Evolution of Lung Cancer Based on the Molecular Characterization of Circulating Tumor Cells using Aptamer Cocktail-Modified Nanosubstrates
Zhu Y, Zou C, Zhang J, Jiang W, Guan F, Tang K, Li S, Li G, Wang J, Ke Z
ACS Appl Mater Interfaces. 2020 Jan 15. doi: 10.1021/acsami.9b22234.

Cav2.3 channels contribute to dopaminergic neuron loss in a model of Parkinson’s disease
Benkert J, Hess S, Roy S, Beccano-Kelly D, Wiederspohn N, Duda J, Simons C, Patil K, Gaifullina A,  Mannal N, Dragicevic E, Spaich D, Müller S, Nemeth J, Hollmann H, Deuter N, Mousba Y, Kubisch C,
Poetschke C, Striessnig J, Pongs O, Schneider T, Wade-Martins R, Patel S, Parlato R, Frank T, Kloppenburg P & Liss B
Nat Commun 10, 5094 (8 Nov 2019) doi:10.1038/s41467-019-12834-x

Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues
Skalicky S, Zwiers PJ, Kuiper T, Schraml E, Hackl M, Molema G
Biotechniques. 2019 Oct 17. doi: 10.2144/btn-2019-0032.

Cancer Research

Cerebral organoid model reveals excessive proliferation of human caudal late interneuron progenitors in Tuberous Sclerosis Complex
Eichmüller OL, Corsini NS, Vértesy Á, Scholl T, Gruber V-E, Peer AM, Chu J, Novatchkova M, Paredes MF, Feucht M, Knoblich JA
bioRxiv, Feb 27, 2020; doi: doi.org/10.1101/2020.02.27.967802

Intraductal papillary neoplasms of the bile duct are consisted of two distinct types specifically associated with clinicopathological features and molecular phenotypes
Aoki Y, Mizuma M, Hata T, Aoki T, Omori Y, Ono Y, Mizukami Y, Unno M, Furukawa T
J Pathol. 2020 Feb 26. doi: 10.1002/path.5398.

Transcriptomic and Proteomic analysis of clear cell foci (CCF) in the human non-cirrhotic liver identifies several differentially expressed genes and proteins with functions in cancer cell biology and glycogen metabolism
Metzendorf C, Wineberger K, Rausch J, Cigiliano A, Calvisi DF, Peters, Sun B, Mennerich D, Kietzmann T, Dombrowski F, Ribback S
bioXRxiv, 10 Feb 2020, 10 Feb 2020

Neurosciences

Synaptotagmin 13 is neuroprotective across motor neuron diseases
Nizzardo M, Taiana M, Rizzo F, Aguila Benitez J, Nijssen J, Allodi I, Melzi V, Bresolin N, Comi GP, Hedlund E, Corti S
Acta Neuropathol. 2020 Feb 17. doi: 10.1007/s00401-020-02133-x.

Neurochemical Characterization of Neurons Expressing Estrogen Receptor β in the Hypothalamic Nuclei of Rats Using in Situ Hybridization and Immunofluorescence
Kanaya M, Higo S, Ozawa H
Int J Mol Sci. 2019 Dec 23;21(1). pii: E115. doi: 10.3390/ijms21010115.

The Western Diet Regulates Hippocampal Microvascular Gene Expression: An Integrated Genomic Analyses in Female Mice.
Nuthikattu S, Milenkovic D, Rutledge J, Villablanca A
Sci Rep. 2019 Dec 13;9(1):19058. doi: 10.1038/s41598-019-55533-9.

Clinical Applications

Proteomics Reveals Extracellular Matrix Injury in the Glomeruli and Tubulointerstitium of Kidney Allografts with Early Antibody-Mediated Rejection
Clotet-Freixas S, McEvoy CM, Batruch I, Van J, Pastrello C, Kotlyar M, Arambewela M, Boshart A, Farkona S, Niu Y, Li Y, Famure O, Bozovic A, Kulasingam V, Chen P, Kim J, Chan E, Moshkelgosha S, Martinu T, Juvet S, Jurisica I, Chruscinski A, John R, Konvalinka A
bioRxiv 2020.03.03.975672; doi: doi.org/10.1101/2020.03.03.975672

Serine Protease Imbalance in the Small Airways and Development of Centrilobular Emphysema in COPD
Uemasu K, Tanabe N, Tanimura K, Hasegawa K, Mizutani T, Hamakawa Y, Sato S, Ogawa E, Thomas MJ, Ikegami M, Muro S, Hirai T, Sato A
Am J Respir Cell Mol Biol. 2020 Feb 26. doi: 10.1165/rcmb.2019-0377OC.

Ichthyosis with confetti caused by new and recurrent mutations in KRT10 associated with varying degrees of keratin 10 mis-localization
Pan Y, Feng C, Wang H, Lee M, Tang Z, Lin Z
Journal of Dermatological Science, Feb 21, 2020; DOI:https://doi.org/10.1016/j.jdermsci.2020.02.005

Developmental Research, Epigenetics & Aging

Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos
Zhou X, Liu Z, Shen K, Zhao P, Sun MX
Nat Commun. 2020 Mar 13;11(1):1366. doi: 10.1038/s41467-020-15189-w

Tsukushi is essential for the development of the inner ear
Miwa T, Ohta K, Ito N, Hattori S, Miyakawa T, Takeo T, Nakagata N, Song WJ, Minoda R
Mol Brain. 2020 Mar 3;13(1):29. doi: 10.1186/s13041-020-00570-z.

Could perturbed fetal development of the ovary contribute to the development of polycystic ovary syndrome in later life?
Hartanti MD, Rosario R, Hummitzsch K, Bastian NA, Hatzirodos N, Bonner WM, Bayne RA, Irving-Rodgers HF, Anderson RA, Rodgers RJ
PLoS One. 2020 Feb 20;15(2):e0229351. doi: 10.1371/journal.pone.0229351. eCollection 2020.

Plant Research

Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos
Zhou X, Liu Z, Shen K, Zhao P, Sun MX
Nat Commun. 2020 Mar 13;11(1):1366. doi: 10.1038/s41467-020-15189-w

Peltate glandular trichomes of Colquhounia vestita harbor diterpenoid acids that contribute to plant adaptation to UV radiation and cold stresses
Tang T, Li CH, Li DS, Jing SX, Hua J, Luo SH, Liu Y, Li SH
Phytochemistry. 2020 Feb 5;172:112285. doi: 10.1016/j.phytochem.2020.112285.

Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis
Chen M, Bui AQ, Goldberg RB
Methods Mol Biol. 2020;2122:205-222. doi: 10.1007/978-1-0716-0342-0_15.

Forensics

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow
Hunt AL, Pierobon M, Baldelli E, Oliver J, Mitchell D, Gist G, Bateman NW, Larry Maxwell G, Petricoin EF, Conrads TP
Clin Proteomics. 2020 Mar 9;17:9. doi: 10.1186/s12014-020-09272-z. eCollection 2020.

Aluminum-induced toxicity in Urochloa brizantha genotypes: A first glance into root Al-apoplastic and -symplastic compartmentation, Al-translocation and antioxidant performance
Furlan F, Borgo L, Silveira Rabêlo FH, Lanzoni Rossi M, Scaglia Linhares F, Pinheiro Martinelli A, Antunes Azevedo R, Lavres J
Chemosphere, Volume 243, March 2020, 125362

Single cell analyses reveal contrasting life strategies of the two main nitrifiers in the ocean
Kitzinger K, Marchant HK, Bristow LA, Herbold CW, Padilla CC, Kidane AT, Littmann S, Daims H, Pjevac P, Stewart FJ, Wagner M, Kuypers MMM
Nat Commun. 2020 Feb 7;11(1):767. doi: 10.1038/s41467-020-14542-3.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.