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Laser Microdissection Publication List

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

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Topics & Tags

DNA Research

Quantitative analysis of p16 methylation in Barrett's carcinogenesis
Chueca E, Valero A, Hördnler C, Puertas A, Carrera P, García-González MA, Strunk M, Lanas A, Piazuelo E
Ann Diagn Pathol. 2020 Jun 17;47:151554. doi: 10.1016/j.anndiagpath.2020.151554.

Clinical and molecular characteristics of mucosal signet-ring cell carcinoma in Helicobacter pyloriuninfected stomach
Kiso M, Urabe Y, Ito M, Masuda K, Boda T, Kotachi T, Hata K, Yorita N, Nagasaki N, Abduwali M, Hiyama Y, Oka S, Tanaka S, Chayama K
BMC Gastroenterology under Review at ResearchSquare

Regional Variation in Epidermal Susceptibility to UV-Induced Carcinogenesis Reflects Proliferative Activity of Epidermal Progenitors
Roy E, Wong HY, Villani R, Rouille T, Salik B, Sim SL, Murigneux V, Stark MS, Fink JL, Soyer HP, Walker G, Lyons JG, Saunders N, Khosrotehrani K
Cell Rep. 2020 Jun 2;31(9):107702. doi: 10.1016/j.celrep.2020.107702.

RNA Research

Lipotoxic Injury Differentially Regulates Brain Microvascular Gene Expression in Male Mice
Nuthikattu S, Milenkovic D, Rutledge JC, Villablanca AC
Nutrients. 2020 Jun 13;12(6):E1771. doi: 10.3390/nu12061771.

Developmental Programming: Sheep Granulosa and Theca Cell-Specific Transcriptional Regulation by Prenatal Testosterone
Puttabyatappa M, Guo X, Dou J, Dumesic D, Bakulski KM, Padmanabhan V
Endocrinology. 2020 Jun 9:bqaa094. doi: 10.1210/endocr/bqaa094.

NADPH oxidase subunit NOXO1 is a target for emphysema treatment in COPD
Seimetz M, Sommer N, Bednorz M, Pak O, Veith C, Hadzic S, Gredic M, Parajuli N, Kojonazarov B, Kraut S, Wilhelm J, Knoepp F, Henneke I, Pichl A, Kanbagli ZI, Scheibe S, Fysikopoulos A Wu, C-Y, Klepetko W, Jaksch P, Eichstaedt C, Grünig E, Hinderhofer K, Geiszt M, Müller N, Rezende F, Buchmann G, Wittig I, Hecker M, Hecker A, Padberg W, Dorfmüller P, Gattenlöhner P, Vogelmeier CF, Günther A, Karnati S, Baumgart-Vogt E, Schermuly RT, Ghofrani HA, Seeger W, Schröder K, Grimminger F, Brandes RP, and Weissmann N
Nature Metabolism (8 Jun 2020). doi.org/10.1038/s42255-020-0215-8

Proteomics, Lipidomics, Metabolomics

Proteomic Analysis Identifies Distinct Glomerular Extracellular Matrix in Collapsing Focal Segmental Glomerulosclerosis
Merchant ML, Barati MT, Caster DJ, Hata JL, Hobeika L, Coventry S, Brier ME, Wilkey DW, Li M, Rood IM, Deegens JK, Wetzels JF, Larsen CP, Troost JP, Hodgin JB, Mariani LH, Kretzler M, Klein JB, McLeish KR
J Am Soc Nephrol. 2020 Jun 19:ASN.2019070696. doi: 10.1681/ASN.2019070696.

Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats
Kadota A, Iwaide S, Miyazaki S, Mitsui I, Machida N, Murakami T
Veterinary Pathology. 17 Jun 2020. doi.org/10.1177/0300985820934113

Paraffin Immunofluorescence Increases Light-Chain Detection in Extra-Renal Light Chain Amyloidosis and Other Light-Chain-Associated Diseases
Gibier JB, Perbet R, Lopez B, Colombat M, Dubois R, Humez S, Terriou L, Copin MC, Gnemmi V
Arch Pathol Lab Med. 2020 Jun 15. doi: 10.5858/arpa.2020-0018-OA.

Fluorescence Applications

Regional Variation in Epidermal Susceptibility to UV-Induced Carcinogenesis Reflects Proliferative Activity of Epidermal Progenitors
Roy E, Wong HY, Villani R, Rouille T, Salik B, Sim SL, Murigneux V, Stark MS, Fink JL, Soyer HP, Walker G, Lyons JG, Saunders N, Khosrotehrani K
Cell Rep. 2020 Jun 2;31(9):107702. doi: 10.1016/j.celrep.2020.107702.

Combined maternal exposure to cypermethrin and stress affect embryonic brain and placental outcomes in mice
Elser BA, Kayali K, Dhakal R, O'Hare B, Wang K, Lehmler HJ, Stevens HE
Toxicol Sci. 2020 Mar 19. pii: kfaa040. doi: 10.1093/toxsci/kfaa040.

Dynamically Monitoring the Clonal Evolution of Lung Cancer Based on the Molecular Characterization of Circulating Tumor Cells using Aptamer Cocktail-Modified Nanosubstrates
Zhu Y, Zou C, Zhang J, Jiang W, Guan F, Tang K, Li S, Li G, Wang J, Ke Z
ACS Appl Mater Interfaces. 2020 Jan 15. doi: 10.1021/acsami.9b22234.

Cancer Research

Quantitative analysis of p16 methylation in Barrett's carcinogenesis
Chueca E, Valero A, Hördnler C, Puertas A, Carrera P, García-González MA, Strunk M, Lanas A, Piazuelo E
Ann Diagn Pathol. 2020 Jun 17;47:151554. doi: 10.1016/j.anndiagpath.2020.151554.

Clinical and molecular characteristics of mucosal signet-ring cell carcinoma in Helicobacter pyloriuninfected stomach
Kiso M, Urabe Y, Ito M, Masuda K, Boda T, Kotachi T, Hata K, Yorita N, Nagasaki N, Abduwali M, Hiyama Y, Oka S, Tanaka S, Chayama K
BMC Gastroenterology under Review at ResearchSquare

Regional Variation in Epidermal Susceptibility to UV-Induced Carcinogenesis Reflects Proliferative Activity of Epidermal Progenitors
Roy E, Wong HY, Villani R, Rouille T, Salik B, Sim SL, Murigneux V, Stark MS, Fink JL, Soyer HP, Walker G, Lyons JG, Saunders N, Khosrotehrani K
Cell Rep. 2020 Jun 2;31(9):107702. doi: 10.1016/j.celrep.2020.107702.

Neurosciences

Lipotoxic Injury Differentially Regulates Brain Microvascular Gene Expression in Male Mice
Nuthikattu S, Milenkovic D, Rutledge JC, Villablanca AC
Nutrients. 2020 Jun 13;12(6):E1771. doi: 10.3390/nu12061771.

GluN2D-mediated excitatory drive onto medial prefrontal cortical PV+ fast-spiking inhibitory interneurons
Garst-Orozco J, Malik R, Lanz TA, Weber ML, Xi H, Arion D, Enwright JF 3rd, Lewis DA, O'Donnell P, Sohal VS, Buhl DL
PLoS One. 2020 Jun 4;15(6):e0233895. doi: 10.1371/journal.pone.0233895. eCollection 2020.

Sparse Activity of Hippocampal Adult-Born Neurons during REM Sleep Is Necessary for Memory Consolidation
Kumar D, Koyanagi I, Carrier-Ruiz A, Vergara P, Srinivasan S, Sugaya Y, Kasuya M, Yu TS, Vogt KE, Muratani M, Ohnishi T, Singh S, Teixeira CM, Chérasse Y, Naoi T, Wang SH, Nondhalee P, Osman BAH, Kaneko N, Sawamoto K, Kernie SG, Sakurai T, McHugh TJ, Kano M, Yanagisawa M, Sakaguchi M
Neuron. 2020 May 20:S0896-6273(20)30354-8. doi: 10.1016/j.neuron.2020.05.008.

Clinical Applications

Proteomic Analysis Identifies Distinct Glomerular Extracellular Matrix in Collapsing Focal Segmental Glomerulosclerosis
Merchant ML, Barati MT, Caster DJ, Hata JL, Hobeika L, Coventry S, Brier ME, Wilkey DW, Li M, Rood IM, Deegens JK, Wetzels JF, Larsen CP, Troost JP, Hodgin JB, Mariani LH, Kretzler M, Klein JB, McLeish KR
J Am Soc Nephrol. 2020 Jun 19:ASN.2019070696. doi: 10.1681/ASN.2019070696.

Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats
Kadota A, Iwaide S, Miyazaki S, Mitsui I, Machida N, Murakami T
Veterinary Pathology. 17 Jun 2020. doi.org/10.1177/0300985820934113

Paraffin Immunofluorescence Increases Light-Chain Detection in Extra-Renal Light Chain Amyloidosis and Other Light-Chain-Associated Diseases
Gibier JB, Perbet R, Lopez B, Colombat M, Dubois R, Humez S, Terriou L, Copin MC, Gnemmi V
Arch Pathol Lab Med. 2020 Jun 15. doi: 10.5858/arpa.2020-0018-OA.

Developmental Research, Epigenetics & Aging

Developmental Programming: Sheep Granulosa and Theca Cell-Specific Transcriptional Regulation by Prenatal Testosterone
Puttabyatappa M, Guo X, Dou J, Dumesic D, Bakulski KM, Padmanabhan V
Endocrinology. 2020 Jun 9:bqaa094. doi: 10.1210/endocr/bqaa094.

Dehydroascorbic acid, the oxidized form of vitamin C, improves renal histology and function in old mice
Forman K, Martínez F, Cifuentes M, Fernández M, Bertinat R, Torres P, Salazar K, Godoy A, Nualart F
J Cell Physiol. 2020 May 21. doi: 10.1002/jcp.29791.

Glutathione peroxidase-1 overexpression reduces oxidative stress, and improves pathology and proteome remodeling in the kidneys of old mice
Chu Y, Lan RS, Huang R, Feng H, Kumar R, Dayal S, Chan K-S, Dai D-F
Aging Cell. 2020 May 13. doi: 10.1111/acel.13154.

Plant Research

Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis
Balestrini R, Fiorilli V
Methods Mol Biol. 2020;2146:171-184. doi: 10.1007/978-1-0716-0603-2_13.

Molecular identifcation of Nectriaceae in infections of apple replant disease afected roots collected by Harris Uni‑Core punching or laser microdissection
Popp C, Wamhoff D, Winkelmann T, Maiss E, Grunewaldt-Stöcker G
Journal of Plant Diseases and Protection, 9 May 2020,  doi.org/10.1007/s41348-020-00333-x

Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos
Zhou X, Liu Z, Shen K, Zhao P, Sun MX
Nat Commun. 2020 Mar 13;11(1):1366. doi: 10.1038/s41467-020-15189-w

Forensics

Compatibility of the ForenSeq™ DNA Signature Prep Kit with laser microdissected cells: An exploration of issues that arise with samples containing low cell numbers
England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison SA
Forensic Science International: Genetics, 1 May 2020, 102278

Application of Optical Trapping to Obtain Single-Source STR Profiles fr ofiles from Forensically Rele ensically Relevant Body Fluid Mixtur ant Body Fluid Mixtures with es with Modified DNA Analysis Workflow
O`Brien BJ
Thesis, 28 April 2020

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Information for practitioners

 

Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells
Zhang Z, and Loudon A
Bio-protocol 10(08): e3590. DOI:10.21769/BioProtoc.3590

Ezrin T567 phosphorylation regulates migration and invasion of ectopic endometrial stromal cells by changing actin cytoskeleton
Chen C, Ye C, Xia J, Zhou Y, Wu R
Life Sci. 2020 May 4:117681. doi: 10.1016/j.lfs.2020.117681.

Cell-type diversity and regionalized gene expression in the planarian intestine
Forsthoefel DJ, Cejda NI, Khan UW, Newmark PA
Elife. 2020 Apr 2;9. pii: e52613. doi: 10.7554/eLife.52613.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.