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Laser Microdissection Publication List

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

Authors

Topics & Tags

DNA Research

van Bladel DAG, van den Brand M, Rijntjes J, Pamidimarri Naga S, Haacke DLCM, Luijks JACW, Hebeda KM, van Krieken JHJM, Groenen PJTA, Scheijen B:
Clonality assessment and detection of clonal diversity in classic Hodgkin lymphoma by next-generation sequencing of immunoglobulin gene rearrangements
Mod Pathol. 2021 Dec 3. doi: 10.1038/s41379-021-00983-8.

Zhu Z, Wang W, Lin F, Jordan T, Li G, Silverman S, Qiu S, Joy AA, Chen C, Hockley DL, Zhang X, Zhou Q, Postovit LM, Zhang X, Hou Y, Mackey JR, Li B, Ka-Shu Wong G: 
Genome profiles of pathologist-defined cell clusters by multiregional LCM and G&T-seq in one triple-negative breast cancer patient
Cell Reports Medicine, Vol 2, Issue 10, 19 Oct 2021, 100404

Onozato Y, Sasaki Y, Abe Y, Sato H, Yagi M, Mizumoto N, Kon T, Sakai T, Ito M, Umehara M, Koseki A, Ueno Y:
Novel genomic alteration in superficial esophageal squamous cell neoplasms in non-smoker non-drinker females
Sci Rep. 2021 Oct 11;11(1):20150. doi: 10.1038/s41598-021-99790-z.

RNA Research

Joseph NF, Zucca A, Wingfield JL, Espadas I, Page D, Puthanveettil SV:
Molecular motor KIF3B in the prelimbic cortex constrains the consolidation of contextual fear memory
Mol Brain. 2021 Nov 8;14(1):162. doi: 10.1186/s13041-021-00873-9.

Zhu Z, Wang W, Lin F, Jordan T, Li G, Silverman S, Qiu S, Joy AA, Chen C, Hockley DL, Zhang X, Zhou Q, Postovit LM, Zhang X, Hou Y, Mackey JR, Li B, Ka-Shu Wong G:
Genome profiles of pathologist-defined cell clusters by multiregional LCM and G&T-seq in one triple-negative breast cancer patient
Cell Reports Medicine, Vol 2, Issue 10, 19 Oct 2021, 100404

Balay SD, Hochstoeger T, Vilceanu A, Malkemper EP, Snider W, Dürnberger G, Mechtler K, Schuechner S, Ogris E, Nordmann GC, Ushakova L, Nimpf S, Keays DA:
The expression, localisation and interactome of pigeon CRY2.
Sci Rep. 2021 Oct 13;11(1):20293. doi: 10.1038/s41598-021-99207-x.

Proteomics, Lipidomics, Metabolomics

Schrader JM, Xu F, Lee H, Barlock B, Benveniste H, Van Nostrand WE:
Emergent White Matter Degeneration in the rTg-DI Rat Model of Cerebral Amyloid Angiopathy Exhibits Unique Proteomic Changes
Am J Pathol. 2021 Dec 8:S0002-9440(21)00526-5. doi: 10.1016/j.ajpath.2021.11.010.

Yamada K, Nakanowatari M, Yumoto E, Satoh S, Asahina M:
Spatiotemporal plant hormone analysis from cryosections using laser microdissection-liquid chromatography-mass spectrometry
J Plant Res. 2021 Nov 23. doi: 10.1007/s10265-021-01360-x.

Sandebring-Matton A, Axenhus M, Bogdanovic N, Winblad B, Schedin-Weiss S, Nilsson P, and Tjernberg LO:
Microdissected Pyramidal Cell Proteomics of Alzheimer Brain Reveals Alterations in Creatine Kinase B-Type, 14-3-3-γ, and Heat Shock Cognate 71
Front. Aging Neurosci., 19 November 2021 | doi.org/10.3389/fnagi.2021.735334

Fluorescence Applications

Sun M, Zheng J, Wnag B and Yao J:
Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
BioMed Research International, Volume 2021 | Article ID 7269237

Tatsumi K, Kinugawa K, Isonishi A, Kitabatake M, Okuda H, Takemura S, Tanaka T, Mori E, Wanaka A:
Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
Mol Brain. 2021 Nov 8;14(1):163. doi: 10.1186/s13041-021-00874-8.

Irizarry BA, Davis J, Zhu X, Boon BDC, Rozemuller AJM, Van Nostrand WE, Smith SO:
Human cerebral vascular amyloid contains both anti-parallel and parallel in-register Aβ40 fibrils
J Biol Chem. 2021 Sep 29:101259. doi: 10.1016/j.jbc.2021.101259.

Cancer Research

van Bladel DAG, van den Brand M, Rijntjes J, Pamidimarri Naga S, Haacke DLCM, Luijks JACW, Hebeda KM, van Krieken JHJM, Groenen PJTA, Scheijen B:
Clonality assessment and detection of clonal diversity in classic Hodgkin lymphoma by next-generation sequencing of immunoglobulin gene rearrangements
Mod Pathol. 2021 Dec 3. doi: 10.1038/s41379-021-00983-8.

Zhu Z, Wang W, Lin F, Jordan T, Li G, Silverman S, Qiu S, Joy AA, Chen C, Hockley DL, Zhang X, Zhou Q, Postovit LM, Zhang X, Hou Y, Mackey JR, Li B, Ka-Shu Wong G:
Genome profiles of pathologist-defined cell clusters by multiregional LCM and G&T-seq in one triple-negative breast cancer patient
Cell Reports Medicine, Vol 2, Issue 10, 19 Oct 2021, 100404

Grünwald BT, Devisme A, Andrieux G, Vyas F, Aliar K, McCloskey CW, Macklin A, Jang GH, Denroche R, Romero JM, Bavi P, Bronsert P, Notta F, O'Kane G, Wilson J, Knox J, Tamblyn L, Udaskin M, Radulovich N, Fischer SE, Boerries M, Gallinger S, Kislinger T, Khokha R:
Spatially confined sub-tumor microenvironments in pancreatic cancer.
Cell. 2021 Oct 7:S0092-8674(21)01105-3. doi: 10.1016/j.cell.2021.09.022.

Neurosciences

Schrader JM, Xu F, Lee H, Barlock B, Benveniste H, Van Nostrand WE:
Emergent White Matter Degeneration in the rTg-DI Rat Model of Cerebral Amyloid Angiopathy Exhibits Unique Proteomic Changes
Am J Pathol. 2021 Dec 8:S0002-9440(21)00526-5. doi: 10.1016/j.ajpath.2021.11.010.

Sandebring-Matton A, Axenhus M, Bogdanovic N, Winblad B, Schedin-Weiss S, Nilsson P, and Tjernberg LO:
Microdissected Pyramidal Cell Proteomics of Alzheimer Brain Reveals Alterations in Creatine Kinase B-Type, 14-3-3-γ, and Heat Shock Cognate 71
Front. Aging Neurosci., 19 November 2021 | doi.org/10.3389/fnagi.2021.735334

Tatsumi K, Kinugawa K, Isonishi A, Kitabatake M, Okuda H, Takemura S, Tanaka T, Mori E, Wanaka A:
Olig2-astrocytes express neutral amino acid transporter SLC7A10 (Asc-1) in the adult brain
Mol Brain. 2021 Nov 8;14(1):163. doi: 10.1186/s13041-021-00874-8.

Clinical/Pathology/Immunology Research Applications

Kaga H, Matsumura H, Suzuki T, Dohmae N, Odaka M, Komatsuda A, Takahashi N, Wakui H:
Proteomic Analysis of Glomerular Proteins in Primary and Bucillamine-induced Membranous Nephropathy
Research Square, 12 Nov 2021, DOI: 10.21203/rs.3.rs-988234/v1 PPR: PPR419553

Said SM, Rocha AB, Valeri AM, Paueksakon P, Dasari S, Theis JD, Vrana JA, Obadina MO, Saghafi D, Alexander MP, Sethi S, Larsen CP, Joly F, Dispenzieri A, Bridoux F, Sirac C, Leung N, Fogo AB, McPhail ED, Nasr SH:
The characteristics of patients with kidney light chain deposition disease concurrent with light chain amyloidosis
Kidney Int. 2021 Nov 9:S0085-2538(21)01035-8. doi: 10.1016/j.kint.2021.10.019.

Ke M, Li X, Wang L, Yue S, Zhao B:
A Stepwise Data Interpretation Process For Renal Amyloidosis Subtyping by LMD-MS
Research Square, 13 Oct 2021, in review for BMC Nephrology

Developmental Research, Epigenetics & Aging

Friess C, Götz M, Kjell J:
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
J Vis Exp. 2021 Oct 7;(176). doi: 10.3791/63047.

Tezuka Y, Eguchi-Ishimae M, Ozaki E, Ito T, Ishii E, Eguchi M:
Activation of fibroblast growth factor-inducible 14 in the early phase of childhood IgA nephropathy
PLoS One. 2021 Oct 1;16(10):e0258090. doi: 10.1371/journal.pone.0258090. eCollection 2021.

Gormley M, Oliverio O, Kapidzic M, Ona K, Hall S, Fisher SJ:
RNA profiling of laser microdissected human trophoblast subtypes at mid-gestation reveals a role for cannabinoid signaling in invasion
Development. 2021 Sep 24:dev.199626. doi: 10.1242/dev.199626.

Plant Research

Yamada K, Nakanowatari M, Yumoto E, Satoh S, Asahina M:
Spatiotemporal plant hormone analysis from cryosections using laser microdissection-liquid chromatography-mass spectrometry
J Plant Res. 2021 Nov 23. doi: 10.1007/s10265-021-01360-x.

Deguchi A, Tatsuzawa F, Ishii K, Abe T, and Miyoshi K:
Localized Repression of Two bHLH Genes is Involved in the Formation of White Margins and White Abaxial Surfaces in Carnation Petals by Inducing the Absence of Anthocyanin Synthesis
The Horticulture Journal Preview, 14 Oct 2021, doi: 10.2503/hortj.UTD-313

Beretta VM, Franchini E, Ud Din I, Lacchini E, Van den Broeck L, Sozzani R, Orozco-Arroyo G, Adam H, Jouannic S, Gregis V, and Kater MM:
The ALOG family members OsG1L1 and OsG1L2 regulate inflorescence branching in rice
Poster, 64th Italian Society of Agrcultural Genetics, Annual Confress, Sep 2021

Forensics

Sun M, Zheng J, Wnag B and Yao J:
Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
BioMed Research International, Volume 2021 | Article ID 7269237

Prajapati S, Rajmane P, Jayakrishna P, Nair MS, Kshirsagar P and Meshram M:
Application and utility of alternative methods in isolation of pure cells from forensic biological mixtures in modern-day: a review
JFSR – Journal of Forensic Science and Research, Aug 2021, ISSN 2575-0186

Chong KWY, Thong Z, Kiu-Choong Syn C :
Recent trends and developments in forensic DNA extraction
WIREs Forensic Science, 30 July 2020, DOI: 10.1002/wfs2.1395

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Further information on Leica LMD impact at forensics: 
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Tripodi L, Molinaro D, Farini A, Cadiao G, Villa C, and Torrente Y:
Flavonoids and Omega3 Prevent Muscle and Cardiac Damage in Duchenne Muscular Dystrophy Animal Model
Cells 2021, 10(11), 2917; doi.org/10.3390/cells10112917

Branigan GL, Olsen KS, Burda I, Haemmerle MW, Ho J, Venuto A, D’Antonio ND, Briggs IE, and DiBenedetto AJ:
Zebrafish Paralogs brd2a and brd2b Are Needed for Proper Circulatory, Excretory and Central Nervous System Formation and Act as Genetic Antagonists during Development
J. Dev. Biol. 2021, 9(4), 46; doi.org/10.3390/jdb9040046

Radic Shechter K, Kafkia E, Zirngibl K, Gawrzak S, Alladin A, Machado D, Lüchtenborg C, Sévin DC, Brügger B, Patil KR, Jechlinger M:
Metabolic memory underlying minimal residual disease in breast cancer
Mol Syst Biol. 2021 Oct;17(10):e10141. doi: 10.15252/msb.202010141.

Live Cell Culture

Hammoudeh SM, Hammoudeh AM, Venkatachalam T, Rawat S, Jayakumar MN, Rahmani M, and Hamoudi R:
Enriched transcriptome analysis of laser capture microdissected populationsof single cells to investigate intracellular heterogeneity in immunostainedFFPE sections
Computational and Structural Biotechnology Journal, 9 Sep 21, S2001-0370(21)00395-0

Malik S, Valdebenito S, D'Amico D, Prideaux B, Eugenin EA:
HIV infection of astrocytes compromises inter-organelle interactions and inositol phosphate metabolism: a potential mechanism of bystander damage and viral reservoir survival
Prog Neurobiol. 2021 Aug 26:102157. doi: 10.1016/j.pneurobio.2021.102157.

Valdebenito S, Malik S, Luu R, Loudig O, Mitchell M, Okafo G, Bhat K, Prideaux B, Eugenin EA:
Tunneling nanotubes, TNT, communicate glioblastoma with surrounding non-tumor astrocytes to adapt them to hypoxic and metabolic tumor conditions
Sci Rep. 2021 Jul 15;11(1):14556. doi: 10.1038/s41598-021-93775-8.

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