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Laser Microdissection Publication List

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

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Topics & Tags

DNA Research

Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer
Arstad C, Taskén K, Refinetti P, Axcrona U, Giercksky K-E, Ekstrøm PO
Prostate Cancer, 28 Aug 2020, Volume 2020, Article ID 7673684

Furan Induced Characteristic Glutathione S-Transferase Placental Form-Positive Foci in Terms of Cell Kinetics and Gene Expression
Takasu S, Ishii Y, Kijima A, Ogawa K, Nakane S, Umemura T
Toxicol Pathol. 2020 Aug 24:192623320948782. doi: 10.1177/0192623320948782.

Granulomatous Inflammation of the Muzzle in White-Tailed Deer (Odocoileus virginianus) and Mule Deer (Odocoileus hemionus) Associated With Mannheimia granulomatis
Keel MK, Keeler S, Brown J, Fenton H, Munk B, Gerhold R, Gottdenker N, Ruder M, Elsmo E, Nemeth N
Vet Pathol. 2020 Aug 19:300985820948825. doi: 10.1177/0300985820948825.

RNA Research

Liraglutide Improves the Kidney Function in a Murine Model of Chronic Kidney Disease
Ougaard ME, Sembach FE, Jensen HE, Pyke C, Knudsen LB, Kvist PH
Nephron. 2020 Sep 2:1-12. doi: 10.1159/000509418.

LCM-seq reveals unique transcriptional adaptation mechanisms of resistant neurons and identifies protective pathways in spinal muscular atrophy
Nichterwitz S, Nijssen J, Storvall H, Schweingruber C, Comley LH, Allodi I, Lee MV, Deng Q, Sandberg R, Hedlund E
Genome Res. 2020 Aug;30(8):1083-1096. doi: 10.1101/gr.265017.120.

The effects of tissue fixation on sequencing and transcript abundance of nucleic acids from microdissected liver samples of smallmouth bass (Micropterus dolomieu)
Walsh HL, Sperry AJ, Blazer VS
PLoS One. 2020 Aug 10;15(8):e0236104. doi: 10.1371/journal.pone.0236104.

Proteomics, Lipidomics, Metabolomics

Homozygous expression of the myofibrillar myopathy-associated p.W2710X filamin C variant reveals major pathomechanisms of sarcomeric lesion formation
Schuld J, Orfanos Z, Chevessier F, Eggers B, Heil L, Uszkoreit J, Unger A, Kirfel G, van der Ven PFM, Marcus K, Linke WA, Clemen CS, Schröder R, Fürst DO
Acta Neuropathol Commun. 2020 Sep 4;8(1):154. doi: 10.1186/s40478-020-01001-9.

Proteomic analysis shows that the main constituent of subepidermal localised cutaneous amyloidosis is not galectin-7
Chapman JR, Liu A, Yi SS, Hernandez E, Ritorto MS, Jungbluth AA, Pulitzer M, Dogan A
Amyloid. 2020 Sep 1;1-7. doi:10.1080/13506129.2020.1811962

A proteogenomic profile of early lung adenocarcinomas by protein co-expression network and genomic alteration analysis
Nishimura T, Nakamura H, Tan KT, Zhuo DW, Fujii K, Koizumi H, Naruki S, Takagi M, Furuya N, Kato Y, Chen SJ, Kato H, Saji H
Sci Rep. 2020 Aug 12;10(1):13604. doi: 10.1038/s41598-020-70578-x.

Fluorescence Applications

Proteomic analysis shows that the main constituent of subepidermal localised cutaneous amyloidosis is not galectin-7
Chapman JR, Liu A, Yi SS, Hernandez E, Ritorto MS, Jungbluth AA, Pulitzer M, Dogan A
Amyloid. 2020 Sep 1;1-7. doi:10.1080/13506129.2020.1811962

Wnt signaling activates MFSD2A to suppress vascular endothelial transcytosis and maintain blood-retinal barrier
Wang Z, Liu CH, Huang S, Fu Z, Tomita Y, Britton WR, Cho SS, Chen CT, Sun Y, Ma JX, and Chen J
Science Advances  28 Aug 2020: Vol. 6, no. 35, eaba7457; DOI: 10.1126/sciadv.aba7457

Inhibition of HDAC increases BDNF expression and promotes neuronal rewiring and functional recovery after brain injury
Sada N, Fujita Y, Mizuta N, Ueno M, Furukawa T, Yamashita T
Cell Death Dis. 2020 Aug 18;11(8):655. doi: 10.1038/s41419-020-02897-w.

Cancer Research

Defining a Distinct Immunotherapy Eligible Subset of Patients with Cancer of Unknown Primary Using Gene Expression Profiling with the 92-Gene Assay
Raghav K, Overman M, Poage GM, Soifer HS, Schnabel CA, Varadhachary GR
Oncologist. 2020 Sep 7. doi: 10.1634/theoncologist.2020-0234.

Somatic Mitochondrial DNA Point Mutations Used as Biomarkers to Demonstrate Genomic Heterogeneity in Primary Prostate Cancer
Arstad C, Taskén K, Refinetti P, Axcrona U, Giercksky K-E, Ekstrøm PO
Prostate Cancer, 28 Aug 2020, Volume 2020, Article ID 7673684

Cytokine-related genes play critical roles in extrafollicular growth of follicular lymphoma cells
Yoshida N, Miyoshi H, Arakawa F, Nakashima K, Kawamoto K, Seto M, Ohshima K
Hematol Oncol. 2020 Aug 24. doi: 10.1002/hon.2795.

Neurosciences

LCM-seq reveals unique transcriptional adaptation mechanisms of resistant neurons and identifies protective pathways in spinal muscular atrophy
Nichterwitz S, Nijssen J, Storvall H, Schweingruber C, Comley LH, Allodi I, Lee MV, Deng Q, Sandberg R, Hedlund E
Genome Res. 2020 Aug;30(8):1083-1096. doi: 10.1101/gr.265017.120.

Inhibition of HDAC increases BDNF expression and promotes neuronal rewiring and functional recovery after brain injury
Sada N, Fujita Y, Mizuta N, Ueno M, Furukawa T, Yamashita T
Cell Death Dis. 2020 Aug 18;11(8):655. doi: 10.1038/s41419-020-02897-w.

TDP-43 transports ribosomal protein mRNA to regulate axonal local translation in neuronal axons
Nagano S, Jinno J, Abdelhamid RF, Jin Y, Shibata M, Watanabe S, Hirokawa S, Nishizawa M, Sakimura K, Onodera O, Okada H, Okada T, Saito Y, Takahashi-Fujigasaki J, Murayama S, Wakatsuki S, Mochizuki H, Araki T
Acta Neuropathol. 2020 Aug 16. doi: 10.1007/s00401-020-02205-y.

Clinical Applications

Homozygous expression of the myofibrillar myopathy-associated p.W2710X filamin C variant reveals major pathomechanisms of sarcomeric lesion formation
Schuld J, Orfanos Z, Chevessier F, Eggers B, Heil L, Uszkoreit J, Unger A, Kirfel G, van der Ven PFM, Marcus K, Linke WA, Clemen CS, Schröder R, Fürst DO
Acta Neuropathol Commun. 2020 Sep 4;8(1):154. doi: 10.1186/s40478-020-01001-9.

Liraglutide Improves the Kidney Function in a Murine Model of Chronic Kidney Disease
Ougaard ME, Sembach FE, Jensen HE, Pyke C, Knudsen LB, Kvist PH
Nephron. 2020 Sep 2:1-12. doi: 10.1159/000509418.

Proteomic analysis shows that the main constituent of subepidermal localised cutaneous amyloidosis is not galectin-7
Chapman JR, Liu A, Yi SS, Hernandez E, Ritorto MS, Jungbluth AA, Pulitzer M, Dogan A
Amyloid. 2020 Sep 1;1-7. doi:10.1080/13506129.2020.1811962

Developmental Research, Epigenetics & Aging

Wnt signaling activates MFSD2A to suppress vascular endothelial transcytosis and maintain blood-retinal barrier
Wang Z, Liu CH, Huang S, Fu Z, Tomita Y, Britton WR, Cho SS, Chen CT, Sun Y, Ma JX, and Chen J
Science Advances  28 Aug 2020: Vol. 6, no. 35, eaba7457; DOI: 10.1126/sciadv.aba7457

Increased predominance of the matured ventricular subtype in embryonic stem cell derived cardiomyocytes in vivo
Ichimura H, Kadota S, Kashihara T, Yamada M, Ito K, Kobayashi H, Tanaka Y, Shiba N, Chuma S, Tohyama S, Seto T, Okada K, Kuwahara K, Shiba Y
Sci Rep. 2020 Jul 17;10(1):11883. doi: 10.1038/s41598-020-68373-9.

Analysis of accessible chromatin landscape in the inner cell mass and trophectoderm of
Yang M, Tao X, Shiny T, Zhao T, Scott RT, Seli E
human blastocysts
Mol Hum Reprod. 2020 Jul 14;gaaa048. doi: 10.1093/molehr/gaaa048.

Plant Research

Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis
Balestrini R, Fiorilli V
Methods Mol Biol. 2020;2146:171-184. doi: 10.1007/978-1-0716-0603-2_13.

Molecular identifcation of Nectriaceae in infections of apple replant disease afected roots collected by Harris Uni‑Core punching or laser microdissection
Popp C, Wamhoff D, Winkelmann T, Maiss E, Grunewaldt-Stöcker G
Journal of Plant Diseases and Protection, 9 May 2020,  doi.org/10.1007/s41348-020-00333-x

Cell lineage-specific transcriptome analysis for interpreting cell fate specification of proembryos
Zhou X, Liu Z, Shen K, Zhao P, Sun MX
Nat Commun. 2020 Mar 13;11(1):1366. doi: 10.1038/s41467-020-15189-w

Forensics

Compatibility of the ForenSeq™ DNA Signature Prep Kit with laser microdissected cells: An exploration of issues that arise with samples containing low cell numbers
England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison SA
Forensic Science International: Genetics, 1 May 2020, 102278

Application of Optical Trapping to Obtain Single-Source STR Profiles fr ofiles from Forensically Rele ensically Relevant Body Fluid Mixtur ant Body Fluid Mixtures with es with Modified DNA Analysis Workflow
O`Brien BJ
Thesis, 28 April 2020

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Information for practitioners

 

Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Hemostasis and thrombosis in zebrafish models of human congenital fibrinogen disorders
Freire Sanz C
Thesis, 2020, 10.13097/archive-ouverte/unige:140844

Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection
Cheng L, Mann SA, Lopez-Beltran A, Chovanec M, Santoni M, Wang M,. Albany C, Adra N, Davidson DD, Cimadamore A, Montironi R, and Zhang S
Methods Mol Biol. 2021;2195:31-47. doi: 10.1007/978-1-0716-0860-9_3.

Biomimicking Fiber Platform with Tunable Stiffness to Study Mechanotransduction Reveals Stiffness Enhances Oligodendrocyte Differentiation but Impedes Myelination through YAP-Dependent Regulation
Ong W, Marinval N, Lin J, Nai MH, Chong YS, Pinese C, Sajikumar S, Lim CT, Ffrench-Constant C, Bechler ME, Chew S
Small. 2020 Aug 12:e2003656. doi: 10.1002/smll.202003656.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.