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Laser Microdissection Publication List

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

Authors

Topics & Tags

DNA Research

A diffusion-like process accommodates new crypts during clonal expansion in human colonic epithelium.
Olpe C, Khamis D, Chukanova M, Skoufou-Papoutsaki N, Kemp R, Marks K, Tatton C, Lindskog C, Nicholson A, Brunton-Sim R, Malhotra S, Hoopen RT, Stanley R, Winton DJ, Morrissey E
Gastroenterology. 2021 Apr 22:S0016-5085(21)00659-4. doi: 10.1053/j.gastro.2021.04.035.

Higher Mutation Burden in High Proliferation Compartments of Heterogeneous Melanoma Tumors
Grzywa TM, Koppolu AA, Paskal W, Klicka K, Rydzanicz M, Wejman J, Ploski R, and Wlodarski P
Int. J. Mol. Sci. 2021, 22(8), 3886; doi.org/10.3390/ijms22083886

Pathways for the development of multiple epithelial types of intraductal papillary mucinous neoplasm of the pancreas
Kobayashi T, Omori Y, Ono Y, Karasaki H, Mizukami Y, Makino N, Motoi F, Unno M, Ueno Y, Furukawa T
J Gastroenterol. 2021 Apr 1. doi: 10.1007/s00535-021-01783-2.

RNA Research

Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
Garshick MS, Nikain C, Tawil M, Pena S, Barrett TJ, Wu BG, Gao Z, Blaser MJ, Fisher EA
Sci Rep. 2021 Apr 26;11(1):8966. doi: 10.1038/s41598-021-88479-y.

Exosomal transfer of osteoclast-derived miRNAs to chondrocytes contributes to osteoarthritis progression
Liu J, Wu X, Lu J, Huang G, Dang L, Zhang H, Zhong C, Zhang Z, Li D, Li F, Liang C, Yu Y, Zhang B-T, Chen L, Lu A and Zhang G
Nature Aging, 15 Apr ’21, volume 1, pages368–384(2021)

Integrative RNA-Seq and H3 Trimethylation ChIP-Seq Analysis of Human Lung Cancer Cells Isolated by Laser-Microdissection
Ong Q, Sakashita S, Hanawa E, Kaneko N, Noguchi M, and Muratani M
Cancers 2021, 13(7), 1719; doi.org/10.3390/cancers13071719

Proteomics, Lipidomics, Metabolomics

Antifeedant, cytotoxic, and anti-inflammatory neo-clerodane diterpenoids in the peltate glandular trichomes and fresh leaves of Ajuga forrestii
Wang Y, Liu Y-C, Li W-Y, Guo K, Liu Y, Li S-H
Phytochemistry, Volume 186, June 2021, 112731

Tissue-specific metabolite profiling of Fallopia multiflora (Heshouwu) and Fallopia multiflora var. angulata by mass spectrometry imaging and laser microdissection combined with UPLC-Q/TOF-MS
Zhao Y, Chu S, Gui S, Qin Y, Xu R, Shan T, Peng H
Journal of Pharmaceutical and Biomedical Analysis, Volume 200, 5 June 2021, 114070

Potential Role of Epithelial Endoplasmic Reticulum Stress and Anterior Gradient Protein 2 Homolog in Crohn's Disease Fibrosis
Vieujean S, Hu S, Bequet E, Salee C, Massot C, Bletard N, Pierre N, Quesada Calvo F, Baiwir D, Mazzucchelli G, De Pauw E, Coimbra Marques C, Delvenne P, Rieder F, Louis E, Meuwis MA
J Crohns Colitis. 2021 Apr 2:jjab061. doi: 10.1093/ecco-jcc/jjab061.

Fluorescence Applications

Tissue-specific metabolite profiling of Fallopia multiflora (Heshouwu) and Fallopia multiflora var. angulata by mass spectrometry imaging and laser microdissection combined with UPLC-Q/TOF-MS
Zhao Y, Chu S, Gui S, Qin Y, Xu R, Shan T, Peng H
Journal of Pharmaceutical and Biomedical Analysis, Volume 200, 5 June 2021, 114070

Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging
Li D, Liu J, Yang C, Tian Y, Yin C, Hu L, Chen Z, Zhao F, Zhang R, Lu A, Zhang G, Qian A
Theranostics. 2021 Mar 20;11(11):5585-5604. doi: 10.7150/thno.54477.

Engineered glycomaterial implants orchestrate large-scale functional repair of brain tissue chronically after severe traumatic brain injury
Latchoumane CV, Betancur MI, Simchick GA, Sun MK, Forghani R, Lenear CE, Ahmed A, Mohankumar R, Balaji N, Mason HD, Archer-Hartmann SA, Azadi P, Holmes PV, Zhao Q, Bellamkonda RV, Karumbaiah L
Sci Adv. 2021 Mar 5;7(10):eabe0207. doi: 10.1126/sciadv.abe0207. Print 2021 Mar.

Cancer Research

A diffusion-like process accommodates new crypts during clonal expansion in human colonic epithelium.
Olpe C, Khamis D, Chukanova M, Skoufou-Papoutsaki N, Kemp R, Marks K, Tatton C, Lindskog C, Nicholson A, Brunton-Sim R, Malhotra S, Hoopen RT, Stanley R, Winton DJ, Morrissey E
Gastroenterology. 2021 Apr 22:S0016-5085(21)00659-4. doi: 10.1053/j.gastro.2021.04.035.

IGHG1 induces EMT in gastric cancer cells by regulating TGF-β/SMAD3 signaling pathway
Li Y, Wang P, Ye D, Bai X, Zeng X, Zhao Q, Zhang Z
Journal of Cancer 2021, Vol. 12; 12(12): 3458-3467. doi: 10.7150/jca.56056

Higher Mutation Burden in High Proliferation Compartments of Heterogeneous Melanoma Tumors
Grzywa TM, Koppolu AA, Paskal W, Klicka K, Rydzanicz M, Wejman J, Ploski R, and Wlodarski P
Int. J. Mol. Sci. 2021, 22(8), 3886; doi.org/10.3390/ijms22083886

Neurosciences

Engineered glycomaterial implants orchestrate large-scale functional repair of brain tissue chronically after severe traumatic brain injury
Latchoumane CV, Betancur MI, Simchick GA, Sun MK, Forghani R, Lenear CE, Ahmed A, Mohankumar R, Balaji N, Mason HD, Archer-Hartmann SA, Azadi P, Holmes PV, Zhao Q, Bellamkonda RV, Karumbaiah L
Sci Adv. 2021 Mar 5;7(10):eabe0207. doi: 10.1126/sciadv.abe0207. Print 2021 Mar.

Disabling VEGF-Response of Purkinje Cells by Downregulation of KDR via miRNA-204-5p
Gehmeyr J, Maghnouj A, Tjaden J, Vorgerd M, Hahn S, Matschke V, Theis V, and Theiss C
Int. J. Mol. Sci. 2021, 22(4), 2173; doi.org/10.3390/ijms22042173

Inhibition of repulsive guidance molecule-a protects dopaminergic neurons in a mouse model of Parkinson's disease
Oda W, Fujita Y, Baba K, Mochizuki H, Niwa H, Yamashita T
Cell Death Dis. 2021 Feb 15;12(2):181. doi: 10.1038/s41419-021-03469-2.

Clinical Applications

Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
Garshick MS, Nikain C, Tawil M, Pena S, Barrett TJ, Wu BG, Gao Z, Blaser MJ, Fisher EA
Sci Rep. 2021 Apr 26;11(1):8966. doi: 10.1038/s41598-021-88479-y.

Potential Role of Epithelial Endoplasmic Reticulum Stress and Anterior Gradient Protein 2 Homolog in Crohn's Disease Fibrosis
Vieujean S, Hu S, Bequet E, Salee C, Massot C, Bletard N, Pierre N, Quesada Calvo F, Baiwir D, Mazzucchelli G, De Pauw E, Coimbra Marques C, Delvenne P, Rieder F, Louis E, Meuwis MA
J Crohns Colitis. 2021 Apr 2:jjab061. doi: 10.1093/ecco-jcc/jjab061.

Donor-Derived ALECT2 Amyloidosis and Recurrent Fibrillary Glomerulonephritis in a Transplant Allograft
Nasr SH, Chavez O, Dasari S, Theis JD, Vrana JA, Fatima H, Fu L, Baliga RS, McPhail ED
Kidney Medicine, 16 Feb 2021, doi.org/10.1016/j.xkme.2020.11.019

Developmental Research, Epigenetics & Aging

Exosomal transfer of osteoclast-derived miRNAs to chondrocytes contributes to osteoarthritis progression
Liu J, Wu X, Lu J, Huang G, Dang L, Zhang H, Zhong C, Zhang Z, Li D, Li F, Liang C, Yu Y, Zhang B-T, Chen L, Lu A and Zhang G
Nature Aging, 15 Apr ’21, volume 1, pages368–384(2021)

Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging
Li D, Liu J, Yang C, Tian Y, Yin C, Hu L, Chen Z, Zhao F, Zhang R, Lu A, Zhang G, Qian A
Theranostics. 2021 Mar 20;11(11):5585-5604. doi: 10.7150/thno.54477.

Development, maturation, and maintenance of human prostate inferred from somatic mutations.
Grossmann S, Hooks Y, Wilson L, Moore L, O'Neill L, Martincorena I, Voet T, Stratton MR, Heer R, Campbell PJ
Cell Stem Cell. 2021 Feb 24:S1934-5909(21)00055-2. doi: 10.1016/j.stem.2021.02.005.

Plant Research

Tissue-specific metabolite profiling of Fallopia multiflora (Heshouwu) and Fallopia multiflora var. angulata by mass spectrometry imaging and laser microdissection combined with UPLC-Q/TOF-MS
Zhao Y, Chu S, Gui S, Qin Y, Xu R, Shan T, Peng H
Journal of Pharmaceutical and Biomedical Analysis, Volume 200, 5 June 2021, 114070

Antifeedant, cytotoxic, and anti-inflammatory neo-clerodane diterpenoids in the peltate glandular trichomes and fresh leaves of Ajuga forrestii
Wang Y, Liu Y-C, Li W-Y, Guo K, Liu Y, Li S-H
Phytochemistry, Volume 186, June 2021, 112731

Laser Microdissection of Specific Stem-Base Tissue Types from Olive Microcuttings for Isolation of High-Quality RNA
Velada I, Menéndez E, Teixeira RT, Cardoso H, and Peixe A
Biology 2021, 10, 209.

Forensics

Recent trends and developments in forensic DNA extraction
Chong KWY, Thong Z, Kiu-Choong Syn C
WIREs Forensic Science, 30 July 2020, DOI: 10.1002/wfs2.1395

Compatibility of the ForenSeq™ DNA Signature Prep Kit with laser microdissected cells: An exploration of issues that arise with samples containing low cell numbers
England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison SA
Forensic Science International: Genetics, 1 May 2020, 102278

Application of Optical Trapping to Obtain Single-Source STR Profiles fr ofiles from Forensically Rele ensically Relevant Body Fluid Mixtur ant Body Fluid Mixtures with es with Modified DNA Analysis Workflow
O`Brien BJ
Thesis, 28 April 2020

Information for practitioners

Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Laser microdissection as a tool to study fungal gene expression in mycorrhizal endosymbiosis
Balestrini R, Perotto S, Fiorilli V
IJM - Italian Journal of Mycology, ISSN 2531-7342 - Vol. 50 (2021): 1-9

AI-driven Deep Visual Proteomics defines cell identity and heterogeneity
Mund A, Coscia F, Hollandi R, Kovacs F, Kriston A, Brunner A-D, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
bioRxiv, 27 Jan 2021, doi: doi: doi.org/10.1101/2021.01.25.427969

Venous Thrombosis and Thrombocyte Activity in Zebrafish Models of Quantitative and Qualitative Fibrinogen Disorders
Fish RJ, Freire C, Di Sanza C, Neerman-Arbez M
Int J Mol Sci. 2021 Jan 11;22(2):E655. doi: 10.3390/ijms22020655.

Live Cell Culture

Methods for Single-Cell Isolation and Preparation
Pensold D, Zimmer-Bensch G
In: Yu B., Zhang J., Zeng Y., Li L., Wang X. (eds) Single-cell Sequencing and Methylation. Advances in Experimental Medicine and Biology, vol 1255.

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

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