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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Table of Content

DNA Research

Heterogeneous components of lung adenocarcinomas confer distinct EGFR mutation and PD-L1 expression
Cai YR, Wu H, Shi X, Dong Y, Chang X, Zhang L, Zhou L, Su D, Yang M
BMC Cancer, Oncology, 20 Nov 2019, DOI:10.21203/rs.2.17531/v1

Clinically relevant molecular subtypes and genomic alteration-independent differentiation in gynecologic carcinosarcoma
Gotoh O, Sugiyama Y, Takazawa Y, Kato K, Tanaka N, Omatsu K, Takeshima N, Nomura H, Hasegawa K, Fujiwara K, Taki M, Matsumura N, Noda T, Mori S
Nat Commun. 2019 Oct 31;10(1):4965. doi: 10.1038/s41467-019-12985-x.

Sex-specific epigenetic alterations of the hypothalamic Agrp-Pomc system do not explain ‘diabesity’ in the offspring of high-fat diet (HFD) overfed maternal rats
Schellong K, Melchior K, Ziska T, Ott R, Henrich W, Rancourt RC, Plagemann A
The Journal of Nutritional Biochemistry, Available online 23 October 2019, 108257

RNA Research

Cav2.3 channels contribute to dopaminergic neuron loss in a model of Parkinson’s disease
Benkert J, Hess S, Roy S, Beccano-Kelly D, Wiederspohn N, Duda J, Simons C, Patil K, Gaifullina A,  Mannal N, Dragicevic E, Spaich D, Müller S, Nemeth J, Hollmann H, Deuter N, Mousba Y, Kubisch C,
Poetschke C, Striessnig J, Pongs O, Schneider T, Wade-Martins R, Patel S, Parlato R, Frank T, Kloppenburg P & Liss B
Nat Commun 10, 5094 (8 Nov 2019) doi:10.1038/s41467-019-12834-x

Both fallopian tube and ovarian surface epithelium are cells-of-origin for high-grade serous ovarian carcinoma
Zhang S, Dolgalev I, Zhang T, Ran H, Levine DA, Neel BG
Nat Commun. 2019 Nov 26;10(1):5367. doi: 10.1038/s41467-019-13116-2.

Platelet regulation of myeloid suppressor of cytokine signaling 3 accelerates atherosclerosis
Barrett TJ, Schlegel M, Zhou F, Gorenchtein M, Bolstorff J, Moore KJ, Fisher EA, Berger JS
Sci Transl Med. 2019 Nov 6;11(517). pii: eaax0481. doi: 10.1126/scitranslmed.aax0481.

Proteomics, Lipidomics, Metabolomics

Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Cahill JF, Kertesz V
Methods Mol Biol. 2020;2064:89-101. doi: 10.1007/978-1-4939-9831-9_7.

Proteomics of human glomerulonephritis by laser microdissection and liquid chromatography-tandem mass spectrometry
Kawata N, Kang D, Aiuchi T, Obama T, Yoshitake O, Shibata T, Takimoto M, Itabe H, Honda K
Nephrology (Carlton). 2019 Nov 9. doi: 10.1111/nep.13676.

Serum amyloid P deposition is a sensitive and specific feature of membranous-like glomerulopathy with masked IgG kappa deposits
Larsen CP, Sharma SG, Caza TN, Kenan DJ, Storey AJ, Edmondson RD, Herzog C, Arthur JM
Kideny International, 17 Oct 2019, KINT 1855, S0085-2538(19)31119-6

Fluorescence Applications

Cav2.3 channels contribute to dopaminergic neuron loss in a model of Parkinson’s disease
Benkert J, Hess S, Roy S, Beccano-Kelly D, Wiederspohn N, Duda J, Simons C, Patil K, Gaifullina A,  Mannal N, Dragicevic E, Spaich D, Müller S, Nemeth J, Hollmann H, Deuter N, Mousba Y, Kubisch C,
Poetschke C, Striessnig J, Pongs O, Schneider T, Wade-Martins R, Patel S, Parlato R, Frank T, Kloppenburg P & Liss B
Nat Commun 10, 5094 (8 Nov 2019) doi:10.1038/s41467-019-12834-x

Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues
Skalicky S, Zwiers PJ, Kuiper T, Schraml E, Hackl M, Molema G
Biotechniques. 2019 Oct 17. doi: 10.2144/btn-2019-0032.

Circulating uromodulin inhibits systemic oxidative stress by inactivating the TRPM2 channel
LaFavers KA, Macedo E, Garimella PS, Lima C, Khan S, Myslinski J, McClintick J, Witzmann FA, Winfree S, Phillips CL, Hato T, Dagher PC, Wu XR, El-Achkar TM, Micanovic R
Sci Transl Med. 2019 Oct 2;11(512). pii: eaaw3639. doi: 10.1126/scitranslmed.aaw3639.

Cancer Research

Both fallopian tube and ovarian surface epithelium are cells-of-origin for high-grade serous ovarian carcinoma
Zhang S, Dolgalev I, Zhang T, Ran H, Levine DA, Neel BG
Nat Commun. 2019 Nov 26;10(1):5367. doi: 10.1038/s41467-019-13116-2.

Heterogeneous components of lung adenocarcinomas confer distinct EGFR mutation and PD-L1 expression
Cai YR, Wu H, Shi X, Dong Y, Chang X, Zhang L, Zhou L, Su D, Yang M
BMC Cancer, Oncology, 20 Nov 2019, DOI:10.21203/rs.2.17531/v1

Intense Expression of EGFR L858R Characterizes the Micropapillary Component and L858R Is Associated with the Risk of Recurrence in pN0M0 Lung Adenocarcinoma with the Micropapillary Component
Kishi N, Ito M, Miyata Y, Kanai A, Handa Y, Tsutani Y, Kushitani K, Takeshima Y, Okada M
Ann Surg Oncol. 2019 Nov 15. doi: 10.1245/s10434-019-07854-2.

Neurosciences

Cav2.3 channels contribute to dopaminergic neuron loss in a model of Parkinson’s disease
Benkert J, Hess S, Roy S, Beccano-Kelly D, Wiederspohn N, Duda J, Simons C, Patil K, Gaifullina A,  Mannal N, Dragicevic E, Spaich D, Müller S, Nemeth J, Hollmann H, Deuter N, Mousba Y, Kubisch C,
Poetschke C, Striessnig J, Pongs O, Schneider T, Wade-Martins R, Patel S, Parlato R, Frank T, Kloppenburg P & Liss B
Nat Commun 10, 5094 (8 Nov 2019) doi:10.1038/s41467-019-12834-x

Spatiotemporally Dependent Vascularization Is Differently Utilized among Neural Progenitor Subtypes during Neocortical Development
Komabayashi-Suzuki M, Yamanishi E, Watanabe C, Okamura M, Tabata H, Iwai R, Ajioka I, Matsushita J, Kidoya H, Takakura N, Okamoto T, Kinoshita K, Ichihashi M, Nagata KI, Ema M, Mizutani KI
Cell Rep. 2019 Oct 29;29(5):1113-1129.e5. doi: 10.1016/j.celrep.2019.09.048.

NCS-1 deficiency affects mRNA-levels of genes involved in regulation of ATP-Synthesis and mitochondrial stress in highly vulnerable Substantia nigra dopaminergic neurons
Liss B, Simons C, Benkert J, Deuter N, Pongs O, Schneider T, Duda J
Front. Mol. Neurosci. | doi: 10.3389/fnmol.2019.00252

Clinical Applications

Neural EGFL like 1 as a potential pro-chondrogenic, anti-inflammatory dual-functional disease-modifying osteoarthritis drug
Li C, Zheng Z, Ha P, Jiang W, Berthiaume EA, Lee S, Mills Z, Pan H, Chen EC, Jiang J, Culiat CT, Zhang X, Ting K, Soo C
Biomaterials, Volume 226, January 2020, 119541

Platelet regulation of myeloid suppressor of cytokine signaling 3 accelerates atherosclerosis
Barrett TJ, Schlegel M, Zhou F, Gorenchtein M, Bolstorff J, Moore KJ, Fisher EA, Berger JS
Sci Transl Med. 2019 Nov 6;11(517). pii: eaax0481. doi: 10.1126/scitranslmed.aax0481.

Serum amyloid P deposition is a sensitive and specific feature of membranous-like glomerulopathy with masked IgG kappa deposits
Larsen CP, Sharma SG, Caza TN, Kenan DJ, Storey AJ, Edmondson RD, Herzog C, Arthur JM
Kideny International, 17 Oct 2019, KINT 1855, S0085-2538(19)31119-6

Developmental Research, Epigenetics & Aging

Spatiotemporally Dependent Vascularization Is Differently Utilized among Neural Progenitor Subtypes during Neocortical Development
Komabayashi-Suzuki M, Yamanishi E, Watanabe C, Okamura M, Tabata H, Iwai R, Ajioka I, Matsushita J, Kidoya H, Takakura N, Okamoto T, Kinoshita K, Ichihashi M, Nagata KI, Ema M, Mizutani KI
Cell Rep. 2019 Oct 29;29(5):1113-1129.e5. doi: 10.1016/j.celrep.2019.09.048.

Sex-specific epigenetic alterations of the hypothalamic Agrp-Pomc system do not explain ‘diabesity’ in the offspring of high-fat diet (HFD) overfed maternal rats
Schellong K, Melchior K, Ziska T, Ott R, Henrich W, Rancourt RC, Plagemann A
The Journal of Nutritional Biochemistry, Available online 23 October 2019, 108257

Coordination of Neuron Production in Mouse and Human Cerebral Cortex by the Homolog of Drosophila Mastermind Protein
Ayoub AE, Dominguez MH, Benoit J, Ortega JA, Radonjic N, Zecevic N, Rakic P
Brain Behav Evol. 2019 Aug 15;93(2-3):152-165. doi: 10.1159/000500494.

Plant Research

Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Cahill JF, Kertesz V
Methods Mol Biol. 2020;2064:89-101. doi: 10.1007/978-1-4939-9831-9_7.

Tissue-Specific Metabolite Profiling on the Different Parts of Bolting and Unbolting Peucedanum praeruptorum Dunn (Qianhu) by Laser Microdissection Combined with UPLC-Q/TOF⁻MS and HPLC⁻DAD
Chen LL, Chu SS, Zhang L, Xie J, Dai M, Wu X, Peng HS
Molecules. 2019 Apr 11;24(7). pii: E1439. doi: 10.3390/molecules24071439.

Cryosection preparation for histological study, gene expression analysis and imaging mass spectrometry
Yoshida M, Sato S, Kawamoto T, Kawamoto K, Saigusa D, Uruno A, Saito R, Matsuo-Ueda M, and Yamamoto H
Journal of Plant Biology Research, 21 Mar 2019, 1(1): 1-7

Forensics

Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.

Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

Aluminum-induced toxicity in Urochloa brizantha genotypes: A first glance into root Al-apoplastic and -symplastic compartmentation, Al-translocation and antioxidant performance
Furlan F, Borgo L, Silveira Rabêlo FH, Lanzoni Rossi M, Scaglia Linhares F, Pinheiro Martinelli A, Antunes Azevedo R, Lavres J
Chemosphere, Volume 243, March 2020, 125362

Nanofibril Organization in Silk Fiber as Inspiration for Ductile and Damage-Tolerant Fiber Design
Lin S, Ye C, Zhang W, Xu A, Chen S, Ren J, and Ling S
Adv. Fiber Mater. (2019). doi.org/10.1007/s42765-019-00013-y

A Specific Mutation in Muc2 Determines Early Dysbiosis in Colitis-Prone Winnie Mice
Liso M, De Santis S, Verna G, Dicarlo M, Calasso M, Santino A, Gigante I, Eri R, Raveenthiraraj S, Sobolewski A, Palmitessa V, Lippolis A, Mastronardi M, Armentano R, Serino G, De Angelis M, Chieppa M
Inflamm Bowel Dis. 2019 Nov 21. pii: izz279. doi: 10.1093/ibd/izz279.

Live Cell Culture

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.