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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Table of Content

DNA Research

Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens
Su Y, Bidlingmaier S, Lee NK, Liu B
Methods Mol Biol. 2018;1701:331-347. doi: 10.1007/978-1-4939-7447-4_18.

Anti-aminoacyl-tRNA synthetase-related myositis and dermatomyositis: clues for differential diagnosis on muscle biopsy
Cerbelli B, Pisano A, Colafrancesco S, Pignataro MG, Biffoni M, Berni S, De Luca A, Riccieri V, Priori R, Valesini G, d'Amati G, Giordano C
Virchows Arch. 2017 Nov 16. doi: 10.1007/s00428-017-2269-x.

Location Matters: Distinct DNA Methylation Patterns in GABAergic Interneuronal Populations from Separate Microcircuits within the Human Hippocampus
Ruzicka WB, Subburaju S, Coyle JT, Benes FM
Hum Mol Genet. 2017 Nov 2. doi: 10.1093/hmg/ddx395.

RNA Research

LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
Nichterwitz S, Benitez JA, Hoogstraaten R, Deng Q, Hedlund E
Methods Mol Biol. 2018;1649:95-110. doi: 10.1007/978-1-4939-7213-5_6.

Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis
Zhang S, Thakare D, Yadegari R
Methods Mol Biol. 2018;1676:153-163. doi: 10.1007/978-1-4939-7315-6_9.

Ultrasound microbubble-mediated transfection of NF-κB decoy oligodeoxynucleotide into gingival tissues inhibits periodontitis in rats in vivo
Yamaguchi H, Ishida Y, Hosomichi J, Suzuki JI, Hatano K, Usumi-Fujita R, Shimizu Y, Kaneko S, Ono T
PLoS One. 2017 Nov 1;12(11):e0186264. doi: 10.1371/journal.pone.0186264. eCollection 2017.

Proteomics

Single Cell Immuno-laser Microdissection Coupled to Label-free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia
García-Berrocoso T, Llombart V, Colàs-Campàs L, Hainard A, Licker V, Penalba A, Ramiro L, Simats A, Bustamante A, Martínez-Saez E, Canals F, Sanchez JC, Montaner J
Mol Cell Proteomics. 2017 Nov 13. pii: mcp.RA117.000419. doi: 10.1074/mcp.RA117.000419.

Valeur prédictive du récepteur NKp30 dans la réponse à l’imatinib mesylate des tumeurs stromales gastrointestinales et identification d’un nouveau mécanisme inhibiteur des cellules Natural Killer par la voie TNFα/TNFR2/BIRC3/TRAF1
Ivagnes A
Thesis (article mainly in French), Oncoimmunology

Gastrin Induces Nuclear Export and Proteasome Degradation of Menin in Enteric Glial Cells
Sundaresan S, Meininger CA, Kang AJ, Photenhauer AL, Hayes MM, Sahoo N, Grembecka J, Cierpicki T, Ding L, Giordano TJ, Else T, Madrigal DJ, Low MJ, Campbell F, Baker A-M, Xu H, Wright NA, Merchant JL
Gastroenterology (2017), doi: 10.1053/j.gastro.2017.08.038.

Fluorescence Applications

Single Cell Immuno-laser Microdissection Coupled to Label-free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia
García-Berrocoso T, Llombart V, Colàs-Campàs L, Hainard A, Licker V, Penalba A, Ramiro L, Simats A, Bustamante A, Martínez-Saez E, Canals F, Sanchez JC, Montaner J
Mol Cell Proteomics. 2017 Nov 13. pii: mcp.RA117.000419. doi: 10.1074/mcp.RA117.000419.

Gastrin Induces Nuclear Export and Proteasome Degradation of Menin in Enteric Glial Cells
Sundaresan S, Meininger CA, Kang AJ, Photenhauer AL, Hayes MM, Sahoo N, Grembecka J, Cierpicki T, Ding L, Giordano TJ, Else T, Madrigal DJ, Low MJ, Campbell F, Baker A-M, Xu H, Wright NA, Merchant JL
Gastroenterology (2017), doi: 10.1053/j.gastro.2017.08.038.

Direct Reprogramming of Resident NG2 Glia into Neurons with Properties of Fast-Spiking Parvalbumin-Containing Interneurons
Pereira M, Birtele M, Shrigley S, Benitez JA, Hedlund E, Parmar M, Ottosson DR
Stem Cell Reports. 2017 Aug 22. pii: S2213-6711(17)30333-8. doi: 10.1016/j.stemcr.2017.07.023.

Cancer Research

A tissue microRNA signature that predicts the prognosis of breast cancer in young women
Hironaka-Mitsuhashi A, Matsuzaki J, Takahashi RU, Yoshida M, Nezu Y, Yamamoto Y, Shiino S, Kinoshita T, Ushijima T, Hiraoka N, Shimizu C, Tamura K, Ochiya T
PLoS One. 2017 Nov 15;12(11):e0187638. doi: 10.1371/journal.pone.0187638. eCollection 2017.

Differential preventive activity of sulindac and atorvastatin in Apc+/Min-FCCCmice with or without colorectal adenomas
Chang W-C, Jackson C, Riel S, Cooper HS, Devarajan K, Hensley HH, Zhou Y, Vanderveer LA, Nguyen MT, Clapper ML
Gut 2017;0:1–9. doi:10.1136/gutjnl-2017-313942

Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens
Su Y, Bidlingmaier S, Lee NK, Liu B
Methods Mol Biol. 2018;1701:331-347. doi: 10.1007/978-1-4939-7447-4_18.

Neurosciences

Intranasally administered IGF-1 inhibits spreading depression in vivo
Grinberg YY1, Zitzow LA2, Kraig RP
Brain Res. 2017 Dec 15;1677:47-57. doi: 10.1016/j.brainres.2017.09.022. Epub 2017 Sep 23.

Astrocyte reactivity and astrogliosis after spinal cord injury
Okada S, Hara M, Kobayakawa K, Matsumoto Y, Nakashima Y
Neurosci Res. 2017 Oct 17. pii: S0168-0102(17)30592-8. doi: 10.1016/j.neures.2017.10.004. Review.

Laser-captured microglia in the Alzheimer’s and Parkinson’s brain reveal unique regional expression profiles and suggest a potential role for hepatitis B in Alzheimer’s brain
Mastroeni D, Nolz J,Sekar S, Delvaux E, Serrano G, Cuyugan L, Liang WS, Beach TG, Rogers J, Coleman PD
Neurobiology of Aging, 2017, ISSN 0197-4580, doi.org/10.1016/j.neurobiolaging.2017.10.019.

Clinical Applications

Anti-aminoacyl-tRNA synthetase-related myositis and dermatomyositis: clues for differential diagnosis on muscle biopsy
Cerbelli B, Pisano A, Colafrancesco S, Pignataro MG, Biffoni M, Berni S, De Luca A, Riccieri V, Priori R, Valesini G, d'Amati G, Giordano C
Virchows Arch. 2017 Nov 16. doi: 10.1007/s00428-017-2269-x.

RNA-sequencing-based comparative analysis of human hepatic progenitor cells and their niche from alcoholic steatohepatitis livers
Ceulemans A, Verhulst S, Van Haele M, Govaere O, Ventura JJ, van Grunsven LA, Roskams T
Cell Death Dis. 2017 Nov 2;8(11):e3164. doi: 10.1038/cddis.2017.543.

Ultrasound microbubble-mediated transfection of NF-κB decoy oligodeoxynucleotide into gingival tissues inhibits periodontitis in rats in vivo
Yamaguchi H, Ishida Y, Hosomichi J, Suzuki JI, Hatano K, Usumi-Fujita R, Shimizu Y, Kaneko S, Ono T
PLoS One. 2017 Nov 1;12(11):e0186264. doi: 10.1371/journal.pone.0186264. eCollection 2017.

Developmental Research, Epigenetics & Aging

Temporally distinct transcriptional regulation of myocyte dedifferentiation and Myofiber growth during muscle regeneration
Louie KW, Saera-Vila A, Kish PE, Colacino JA, Kahana A
BMC Genomics. 2017 Nov 9;18(1):854. doi: 10.1186/s12864-017-4236-y.

Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis
Zhang S, Thakare D, Yadegari R
Methods Mol Biol. 2018;1676:153-163. doi: 10.1007/978-1-4939-7315-6_9.

LEC1 sequentially regulates the transcription of genes involved in diverse developmental processes during seed development
Pelletier JM, Kwong RW, Park S, Le BH, Baden R, Cagliari A, Hashimoto M, Munoz MD, Fischer RL, Goldberg RB, Harada JJ
Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):E6710-E6719. doi: 10.1073/pnas.1707957114. Epub 2017 Jul 24.

Plant Research

Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis
Zhang S, Thakare D, Yadegari R
Methods Mol Biol. 2018;1676:153-163. doi: 10.1007/978-1-4939-7315-6_9

Laser Capture Microdissection for Amplification of Alternative Oxidase (AOX) Genes in Target Tissues in Daucus carota L.
Ragonezi C, Arnholdt-Schmitt B
Methods Mol Biol. 2017;1670:245-252. doi: 10.1007/978-1-4939-7292-0_21.

Laser microdissection hyphenated with high performance gel permeation chromatography-charged aerosol detector and ultra performance liquid chromatography-triple quadrupole mass spectrometry for histochemical analysis of polysaccharides in herbal medicine: Ginseng, a case study
Chen QL, Chen YJ, Zhou SS, Yip KM, Xu J, Chen HB, Zhao ZZ
Int J Biol Macromol. 2017 Aug 31. pii: S0141-8130(17)32336-X. doi: 10.1016/j.ijbiomac.2017.08.162.

Forensics

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017

Forensic Biology – Expert Evidence
Gunn P, Roebuck H, Summerell A
Bookchapter, reviewed March 2017

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/

Miscellaneous

3D Maskless Micropatterning for Regeneration of Highly Organized Tubular Tissues
Ma C, Qu T, Chang B, Jing Y, Feng JQ, Liu X
Adv Healthc Mater. 2017 Nov 9. doi: 10.1002/adhm.201700738.

Combined loss of EAF2 and p53 induces prostate carcinogenesis in male mice
Wang Y, Pascal LE, Zhong M, Ai J, Wang D, Jing Y, Pilch J, Song Q, Rigatti LH, Graham LE, Nelson JB, Parwani AV, Wang Z
Endocrinology. 2017 Sep 27. doi: 10.1210/en.2017-00409.

Biogeography of cryoconite forming cyanobacteria on polar and Asian glaciers
Segawa T, Yonezawa T, Edwards A, Akiyoshi A, Tanaka S, Uetake J, Irvine-Fynn T, Fukui K, Li Z, Takeuchi N
Journal of Biogeography, 2017 Sep 9, DOI: 10.1111/jbi.13089

Live Cell Culture

Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.

A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.