DNA Research
Frequent SLC35A2 brain mosaicism in mild malformation of cortical development with oligodendroglial hyperplasia in epilepsy (MOGHE)
Bonduelle T, Hartlieb T, Baldassari S, Sim NS, Kim SH, Kang HC, Kobow K, Coras R, Chipaux M, Dorfmüller G, Adle-Biassette H, Aronica E, Lee JH, Blumcke I, Baulac S
Acta Neuropathol Commun. 2021 Jan 6;9(1):3. doi: 10.1186/s40478-020-01085-3.
Endometrial cancer with a POLE mutation progresses frequently through the type I pathway despite its high-grade endometrioid morphology: a cohort study at a single institution in Japan
Monsur M, Yamaguchi M, Tashiro H, Yoshinobu K, Saito F, Erdenebaatar C, Li C, Iwagoi Y, Ohba T, Iyama KI, Katabuchi H
Med Mol Morphol. 2021 Jan 5. doi: 10.1007/s00795-020-00273-3.
Reliable detection of somatic mutations in solid tissues by laser-capture microdissection and low-input DNA sequencing
Ellis P, Moore L, Sanders MA, Butler TM, Brunner SF, Lee-Six H, Osborne R, Farr B, Coorens THH, Lawson ARJ, Cagan A, Stratton MR, Martincorena I, Campbell PJ
Nat Protoc. 2020 Dec 14. doi: 10.1038/s41596-020-00437-6.
RNA Research
Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs
Wang F, Zhao S, Deng D, Wang W, Xu X, Liu X, Zhao S, Yu M
Int J Mol Sci. 2021 Jan 27;22(3):1248. doi: 10.3390/ijms22031248.
Tumour inflammation signature and expression of S100A12 and HLA class I improve survival in HPV-negative hypopharyngeal cancer.
Mints M, Landin D, Näsman A, Mirzaie L, Ursu RG, Zupancic M, Marklund L, Dalianis T, Munck-Wikland E, Ramqvist T
Sci Rep. 2021 Jan 19;11(1):1782. doi: 10.1038/s41598-020-80226-z.
Short prolactin isoforms are expressed in photoreceptors of canine retinas undergoing retinal degeneration
Sudharsan R, Murgiano L, Tang HY, Olsen TW, Chavali VRM, Aguirre GD, Beltran WA.
Sci Rep. 2021 Jan 11;11(1):460. doi: 10.1038/s41598-020-80691-6.
Proteomics, Lipidomics, Metabolomics
AI-driven Deep Visual Proteomics defines cell identity and heterogeneity
Mund A, Coscia F, Hollandi R, Kovacs F, Kriston A, Brunner A-D, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
bioRxiv, 27 Jan 2021, doi: doi: doi.org/10.1101/2021.01.25.427969
The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease
Hondius DC, Koopmans F, Leistner C, Pita-Illobre D, Peferoen-Baert RM, Marbus F, Paliukhovich I, Li KW, Rozemuller AJM, Hoozemans JJM, Smit AB
Acta Neuropathol. 2021 Jan 25. doi: 10.1007/s00401-020-02261-4.
Protein co-expression networks identified from HOT lesions of ER+HER2-Ki-67high luminal breast carcinomas
Yamada K, Nishimura T, Wakiya M, Satoh E, Fukuda T, Amaya K, Bando Y, Hirano H, Ishikawa T
Sci Rep. 2021 Jan 18;11(1):1705. doi: 10.1038/s41598-021-81509-9.
Fluorescence Applications
Alterations of Dopamine-Related Transcripts in A11 Diencephalospinal Pathways after Spinal Cord Injury
Zhao S, DeFinis JH, and Hou S
Neural Plasticity, 15 Jan 2021, Volume 2021, Article ID 8838932, 12 pages
Mechanical forces switch blood vessel subtypes to arrest adolescent bone growth
Dzamukova M, Brunner TM, Miotla-Zarebska J, Heinrich F, Brylka L, Mashreghi M-F, Anjali Kusumbe, Kühn R, Schinke T, Vincent TL, and Löhning M
Reasearch Square, V1, 12 Jan 2021, DOI: 10.21203/rs.3.rs-120475/v1
Venous Thrombosis and Thrombocyte Activity in Zebrafish Models of Quantitative and Qualitative Fibrinogen Disorders
Fish RJ, Freire C, Di Sanza C, Neerman-Arbez M
Int J Mol Sci. 2021 Jan 11;22(2):E655. doi: 10.3390/ijms22020655.
Cancer Research
Tumour inflammation signature and expression of S100A12 and HLA class I improve survival in HPV-negative hypopharyngeal cancer.
Mints M, Landin D, Näsman A, Mirzaie L, Ursu RG, Zupancic M, Marklund L, Dalianis T, Munck-Wikland E, Ramqvist T
Sci Rep. 2021 Jan 19;11(1):1782. doi: 10.1038/s41598-020-80226-z.
Protein co-expression networks identified from HOT lesions of ER+HER2-Ki-67high luminal breast carcinomas
Yamada K, Nishimura T, Wakiya M, Satoh E, Fukuda T, Amaya K, Bando Y, Hirano H, Ishikawa T
Sci Rep. 2021 Jan 18;11(1):1705. doi: 10.1038/s41598-021-81509-9.
Genomic Sequencing of Cancer-related Genes in Sinonasal Squamous Cell Carcinoma and Coexisting Inverted Papilloma
Uchi R, Jiromaru R, Yasumatsu R, Yamamoto H, Hongo T, Manako T, Sato K, Hashimoto K, Wakasaki T, Matsuo M, Nakagawa T
Anticancer Res. 2021 Jan;41(1):71-79. doi: 10.21873/anticanres.14752.
Neurosciences
The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease
Hondius DC, Koopmans F, Leistner C, Pita-Illobre D, Peferoen-Baert RM, Marbus F, Paliukhovich I, Li KW, Rozemuller AJM, Hoozemans JJM, Smit AB
Acta Neuropathol. 2021 Jan 25. doi: 10.1007/s00401-020-02261-4.
Alterations of Dopamine-Related Transcripts in A11 Diencephalospinal Pathways after Spinal Cord Injury
Zhao S, DeFinis JH, and Hou S
Neural Plasticity, 15 Jan 2021, Volume 2021, Article ID 8838932, 12 pages
Gradient of brain mosaic RHEB variants causes a continuum of cortical dysplasia.
Lee WS, Baldassari S, Chipaux M, Adle-Biassette H, Stephenson SEM, Maixner W, Harvey AS, Lockhart PJ, Baulac S, Leventer RJ
Ann Clin Transl Neurol. 2021 Jan 12. doi: 10.1002/acn3.51286.
Clinical Applications
The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease
Hondius DC, Koopmans F, Leistner C, Pita-Illobre D, Peferoen-Baert RM, Marbus F, Paliukhovich I, Li KW, Rozemuller AJM, Hoozemans JJM, Smit AB
Acta Neuropathol. 2021 Jan 25. doi: 10.1007/s00401-020-02261-4.
Therapeutic potential of Fosmanogepix (APX001) for intra-abdominal candidiasis: from lesion penetration to efficacy in a mouse model
Lee A, Wang N, Carter CL, Zimmerman M, Dartois V, Shaw KJ, Perlin DS, Zhao Y
Antimicrob Agents Chemother. 2021 Jan 19:AAC.02476-20. doi: 10.1128/AAC.02476-20.
Distribution of amyloidosis subtypes based on tissue biopsy site - Consecutive analysis of 729 patients at a single amyloidosis center in Japan
Abe R, Katoh N, Takahashi Y, Takasone K, Yoshinaga T, Yazaki M, Kametani F, Sekijima Y
Pathol Int. 2020 Oct 28. doi: 10.1111/pin.13041.
Developmental Research, Epigenetics & Aging
Integrating LCM-Based Spatio-Temporal Transcriptomics Uncovers Conceptus and Endometrial Luminal Epithelium Communication that Coordinates the Conceptus Attachment in Pigs
Wang F, Zhao S, Deng D, Wang W, Xu X, Liu X, Zhao S, Yu M
Int J Mol Sci. 2021 Jan 27;22(3):1248. doi: 10.3390/ijms22031248.
Somatic mutations reveal widespread mosaicism and mutagenesis in human placentas
Coorens THH, Oliver TRW, Sanghvi R, Sovio U, Cook E, Vento-Torno R, Haniffa M, Young MD, Rahbari R, Sebire N, Campbell PJ, Charnock-Jones DS, Smith GCS, Behjati S
bioRxiv, 27 Jan 2021, doi: doi.org/10.1101/2021.01.26.428217
Mechanical forces switch blood vessel subtypes to arrest adolescent bone growth
Dzamukova M, Brunner TM, Miotla-Zarebska J, Heinrich F, Brylka L, Mashreghi M-F, Anjali Kusumbe, Kühn R, Schinke T, Vincent TL, and Löhning M
Reasearch Square, V1, 12 Jan 2021, DOI: 10.21203/rs.3.rs-120475/v1
Plant Research
CcLBD25 functions as a key regulator of haustorium development in Cuscuta campestris
Jhu M-Y, Ichihashi Y, Farhi M, Wong C, Sinha NR
bioRxiv, Jan 2021, doi: doi.org/10.1101/2021.01.04.425251
A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots
Mounier T, Navarro-Sanz S, Bureau C, Antoine L, Varoquaux F, Durandet F, Périn C
BMC Mol Cell Biol. 2020 Dec 10;21(1):92. doi: 10.1186/s12860-020-00312-y.
Spatial transcriptional signatures define margin morphogenesis along the proximal-distal and medio-lateral axes in tomato (Solanum lycopersicum) leaves
Martinez CC, Li S, Woodhouse MR, Sugimoto K, Sinha NR
The Plant Cell, koaa012, doi.org/10.1093/plcell/koaa012
Forensics
Recent trends and developments in forensic DNA extraction
Chong KWY, Thong Z, Kiu-Choong Syn C
WIREs Forensic Science, 30 July 2020, DOI: 10.1002/wfs2.1395
Compatibility of the ForenSeq™ DNA Signature Prep Kit with laser microdissected cells: An exploration of issues that arise with samples containing low cell numbers
England R, Nancollis G, Stacey J, Sarman A, Min J, Harbison SA
Forensic Science International: Genetics, 1 May 2020, 102278
Application of Optical Trapping to Obtain Single-Source STR Profiles fr ofiles from Forensically Rele ensically Relevant Body Fluid Mixtur ant Body Fluid Mixtures with es with Modified DNA Analysis Workflow
O`Brien BJ
Thesis, 28 April 2020
Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.
Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/
Miscellaneous
AI-driven Deep Visual Proteomics defines cell identity and heterogeneity
Mund A, Coscia F, Hollandi R, Kovacs F, Kriston A, Brunner A-D, Bzorek M, Naimy S, Rahbeck Gjerdum LM, Dyring-Andersen B, Bulkescher JM, Lukas C, Gnann C, Lundberg E, Horvath P, Mann M
bioRxiv, 27 Jan 2021, doi: doi: doi.org/10.1101/2021.01.25.427969
Venous Thrombosis and Thrombocyte Activity in Zebrafish Models of Quantitative and Qualitative Fibrinogen Disorders
Fish RJ, Freire C, Di Sanza C, Neerman-Arbez M
Int J Mol Sci. 2021 Jan 11;22(2):E655. doi: 10.3390/ijms22020655.
Auto-qPCR: A Python based web app for automated and reproducible analysis of qPCR data
Maussion G, Thomas RA, Demirova I, Gu G, Cai E, Chen CX-Q, Abdian N, Strauss TJP, Kelaï S, Nauleau-Javaudin A, Beitel LK, Ramoz N, Gorwood P, Durcan TM
bioRxiv, Jan 2021, doi: doi.org/10.1101/2021.01.14.426748
Live Cell Culture
Methods for Single-Cell Isolation and Preparation
Pensold D, Zimmer-Bensch G
In: Yu B., Zhang J., Zeng Y., Li L., Wang X. (eds) Single-cell Sequencing and Methylation. Advances in Experimental Medicine and Biology, vol 1255.
Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.
Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.
