DNA Research
Modulation of mtDNA copy number ameliorates the pathological consequences of a heteroplasmic mtDNA mutation in the mouse
Filograna R, Koolmeister C, Upadhyay M, Pajak A, Clemente P, Wibom R, Simard ML, Wredenberg A, Freyer C, Stewart JB, Larsson NG
Sci Adv. 2019 Apr 3;5(4):eaav9824. doi: 10.1126/sciadv.aav9824. eCollection 2019 Apr.
Molecular association of functioning stroma with carcinoma cells in the ovary: A preliminary study
Narikiyo M, Yano M, Kamada K, Katoh T, Ito K, Shuto M, Kayano H, Yasuda M
Oncol Lett. 2019 Mar;17(3):3562-3568. doi: 10.3892/ol.2019.9992.
dPCR Mutational Analyses in Cell-Free DNA: A Comparison with Tissues
Takeshita T, Iwase H
Methods Mol Biol. 2019;1909:105-118. doi: 10.1007/978-1-4939-8973-7_8.
RNA Research
Effects of ATRAP in Renal Proximal Tubules on Angiotensin-Dependent Hypertension
Kinguchi S, Wakui H, Azushima K, Haruhara K, Koguchi T, Ohki K, Uneda K, Matsuda M, Haku S, Yamaji T, Yamada T, Kobayashi R, Minegishi S, Ishigami T, Yamashita A, Fujikawa T, Tamura K
J Am Heart Assoc. 2019 Apr 16;8(8):e012395. doi: 10.1161/JAHA.119.012395
A conserved CCM complex promotes apoptosis non-autonomously by regulating zinc homeostasis
Chapman EM, Lant B, Ohashi Y, Yu B, Schertzberg M, Go C, Dogra D, Koskimäki J, Girard R, Li Y, Fraser AG, Awad IA, Abdelilah-Seyfried S, Gingras AC, Derry W
Nat Commun. 2019 Apr 17;10(1):1791. doi: 10.1038/s41467-019-09829-z.
Profibrotic epithelial phenotype: a central role for MRTF and TAZ
Bialik JF, Ding M, Speight P, Dan Q, Miranda MZ, Di Ciano-Oliveira C, Kofler MM, Rotstein OD, Pedersen SF, Szászi K, Kapus A
Sci Rep. 2019 Mar 13;9(1):4323. doi: 10.1038/s41598-019-40764-7.
Proteomics, Lipidomics, Metabolomics
Tissue distribution and penetration of isavuconazole at the site of infection in Experimental Invasive Aspergillosis in Mice with underlying Chronic Granulomatous Disease
Lee A, Prideaux B, Lee MH, Zimmerman M, Dolgov E, Perlin DS, Zhao
Antimicrob Agents Chemother. 2019 Apr 15. pii: AAC.00524-19. doi: 10.1128/AAC.00524-19.
Proteomic analysis for the diagnosis of fibrinogen Aα-chain amyloidosis
Taylor GW, Gilbertson JA, Sayed R, Blanco A, Rendell NB, RowczenioD, Rezk T, Mangione PP, Canetti D, Bass P, Hawkins PN, Gillmore JD
Kidney International Reports (2019 Apr 15), doi: doi.org/10.1016/j.ekir.2019.04.007.
Novel biomarkers distinguishing pancreatic head Cancer from distal cholangiocarcinoma based on proteomic analysis
Takenami T, Maeda S, Karasawa H, Suzuki T, Furukawa T, Morikawa T, Takadate T, Hayashi H, Nakagawa K, Motoi F, Naitoh T, Unno M
BMC Cancer. 2019 Apr 5;19(1):318. doi: 10.1186/s12885-019-5548-x.
Fluorescence Applications
Tissue-Specific Metabolite Profiling on the Different Parts of Bolting and Unbolting Peucedanum praeruptorum Dunn (Qianhu) by Laser Microdissection Combined with UPLC-Q/TOF⁻MS and HPLC⁻DAD
Chen LL, Chu SS, Zhang L, Xie J, Dai M, Wu X, Peng HS
Molecules. 2019 Apr 11;24(7). pii: E1439. doi: 10.3390/molecules24071439.
Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Swieck K, Conta-Steencken A, Middleton FA, Siebert JR, Osterhout DJ, Stelzner DJ
BMC Neurosci. 2019 Mar 18;20(1):10. doi: 10.1186/s12868-019-0491-y.
Pinealectomy or light exposure exacerbates biliary damage and liver fibrosis in cholestatic rats through decreased melatonin synthesis
Chen L, Zhou T, Wu N, O’Brien A, Venter J, Ceci L, Kyritsi K, Onori P, Gaudio E, Sybenga A, Xie L, Wu C, Fabris L, Invernizzi P, Zawieja D, Liangpunsakul S, Meng F, Francis H, Glaser S
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 16 Mar 2019, doi.org/10.1016/j.bbadis.2019.03.002
Cancer Research
Clonotypic heterogeneity in cutaneous T-cell lymphoma (mycosis fungoides) revealed by comprehensive whole-exome sequencing
Iyer A, Hennessey D, O'Keefe S, Patterson J, Wang W, Salopek T, Wong GK, Gniadecki R
Blood Adv. 2019 Apr 9;3(7):1175-1184. doi: 10.1182/bloodadvances.2018027482.
Novel biomarkers distinguishing pancreatic head Cancer from distal cholangiocarcinoma based on proteomic analysis
Takenami T, Maeda S, Karasawa H, Suzuki T, Furukawa T, Morikawa T, Takadate T, Hayashi H, Nakagawa K, Motoi F, Naitoh T, Unno M
BMC Cancer. 2019 Apr 5;19(1):318. doi: 10.1186/s12885-019-5548-x.
Investigation of the clonal origin of multifocal papillary thyroid carcinoma according to the X‑chromosome inactivation pattern
Chen D, Qi W, Feng L, Wang J, Wang L, Guan H
Oncology Letters 17, no. 5 (2019): 4695-4700. doi.org/10.3892/ol.2019.10105
https://www.spandidos-publications.com/10.3892/ol.2019.9992
Neurosciences
Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury
Swieck K, Conta-Steencken A, Middleton FA, Siebert JR, Osterhout DJ, Stelzner DJ
BMC Neurosci. 2019 Mar 18;20(1):10. doi: 10.1186/s12868-019-0491-y.
Exercise-induced enhancement of synaptic function triggered by the inverse BAR protein, Mtss1L
Chatzi C, Zhang G, Hendricks W, Chen Y, Schnell E, Goodman RH, Westbrook GL
bioRxiv, Feb. 9, 2019, dx.doi.org/10.1101/545582doi:
Neuropilin-1-mediated pruning of corticospinal tract fibers is required for motor recovery after spinal cord injury
Nakanishi T, Fujita Y, Yamashita T
Cell Death Dis. 2019 Jan 25;10(2):67. doi: 10.1038/s41419-019-1338-2.
Clinical Applications
Proteomic analysis for the diagnosis of fibrinogen Aα-chain amyloidosis
Taylor GW, Gilbertson JA, Sayed R, Blanco A, Rendell NB, RowczenioD, Rezk T, Mangione PP, Canetti D, Bass P, Hawkins PN, Gillmore JD
Kidney International Reports (2019 Apr 15), doi: doi.org/10.1016/j.ekir.2019.04.007.
Tissue distribution and penetration of isavuconazole at the site of infection in Experimental Invasive Aspergillosis in Mice with underlying Chronic Granulomatous Disease
Lee A, Prideaux B, Lee MH, Zimmerman M, Dolgov E, Perlin DS, Zhao
Antimicrob Agents Chemother. 2019 Apr 15. pii: AAC.00524-19. doi: 10.1128/AAC.00524-19.
Modulation of mtDNA copy number ameliorates the pathological consequences of a heteroplasmic mtDNA mutation in the mouse
Filograna R, Koolmeister C, Upadhyay M, Pajak A, Clemente P, Wibom R, Simard ML, Wredenberg A, Freyer C, Stewart JB, Larsson NG
Sci Adv. 2019 Apr 3;5(4):eaav9824. doi: 10.1126/sciadv.aav9824. eCollection 2019 Apr.
Developmental Research, Epigenetics & Aging
A conserved CCM complex promotes apoptosis non-autonomously by regulating zinc homeostasis
Chapman EM, Lant B, Ohashi Y, Yu B, Schertzberg M, Go C, Dogra D, Koskimäki J, Girard R, Li Y, Fraser AG, Awad IA, Abdelilah-Seyfried S, Gingras AC, Derry W
Nat Commun. 2019 Apr 17;10(1):1791. doi: 10.1038/s41467-019-09829-z.
Hypothalamic insulin receptor expression and DNA promoter methylation are sex-specifically altered in adult offspring of high-fat diet (HFD)-overfed mother rats
Schellong K, Melchior K, Ziska T, Ott R, Henrich W, Rancourt RC, Plagemann A
The Journal of Nutritional Biochemistry, 10 Feb 2019, doi.org/10.1016/j.jnutbio.2019.01.014
Thermal stress induces glycolytic beige fat formation via a myogenic state
Chen Y, Ikeda K, Yoneshiro T, Scaramozza A, Tajima K, Wang Q, Kim K, Shinoda K, Sponton CH, Brown Z, Brack A, Kajimura S
Nature. 2019 Jan;565(7738):180-185. doi: 10.1038/s41586-018-0801-z.
Plant Research
Tissue-Specific Metabolite Profiling on the Different Parts of Bolting and Unbolting Peucedanum praeruptorum Dunn (Qianhu) by Laser Microdissection Combined with UPLC-Q/TOF⁻MS and HPLC⁻DAD
Chen LL, Chu SS, Zhang L, Xie J, Dai M, Wu X, Peng HS
Molecules. 2019 Apr 11;24(7). pii: E1439. doi: 10.3390/molecules24071439.
Transcriptome analysis of Rlm2-mediated host immunity in the Brassica napus-Leptosphaeria maculans pathosystem
Becker MG, Haddadi P, Wan J, Adam L, Walker P, Larkan N, Daayf F, Borhan MH, Belmonte MF
Mol Plant Microbe Interact. 2019 Apr 2. doi: 10.1094/MPMI-01-19-0028-R.
Intrusive Growth of Phloem Fibers in Flax Stem: Integrated Analysis of miRNA and mRNA Expression Profiles
Gorshkov O, Chernova T, Mokshina N, Gogoleva N, Suslov D, Tkachenko A, Gorshkova T
Plants (Basel). 2019 Feb 19;8(2). pii: E47. doi: 10.3390/plants8020047.
Forensics
Cold Case Homicides: DNA Testing of Retained Autopsy Sexual Assault Smears
Clark M, Gill J, Sasinouski K, McGuire A
J Forensic Sci. 2019 Feb 7. doi: 10.1111/1556-4029.14023.
Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review)
Tao R, Wang S, Zhang J, Zhang J, Yang Z, Sheng X, Hou Y, Zhang S, Li C
Int J Legal Med. 2018 May 25. doi: 10.1007/s00414-018-1862-0.
Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017
http://www.advocacymatters.com.au/images/CH-81---Forensic-biology.pdf
Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.
Further information on Leica LMD impact at forensics:
www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/
Miscellaneous
Optimized cutting laser trajectory for laser capture microdissection
Korabecna M, Tonar Z, Tomori Z, Demjen E
Biologia (2019). doi.org/10.2478/s11756-019-00234-x
Relationship between Mg, B and Mn status and tomato tolerance against Cd toxicity
Carvalho MEA, Piotto FA, Franco MR, Rossi ML, Martinelli AP, Cuypers A, Azevedo RA
J Environ Manage. 2019 Mar 28;240:84-92. doi: 10.1016/j.jenvman.2019.03.026.
Mitochondria and oxidative stress in ovarian endometriosis
Chen C, Zhou Y, Hu C, Wang Y, Yan Z, Li Z, Wu R
Free Radic Biol Med. 2019 Mar 26. pii: S0891-5849(19)30264-3. doi: 10.1016/j.freeradbiomed.2019.03.027.
Live Cell Culture
Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis
Zhou X, Shi C, Zhao P, Sun M
Plant Reprod. 2018 Dec 13. doi: 10.1007/s00497-018-00353-6.
Long-distance electron transport in individual, living cable bacteria
Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
Proc Natl Acad Sci U S A. 2018 May 7. pii: 201800367. doi: 10.1073/pnas.1800367115.
A novel ex vivo Huntington's disease model for studying GABAergic neurons and cell grafts by laser microdissection
André EM, Daviaud N, Sindji L, Cayon J, Perrot R, Montero-Menei CN
PLoS One. 2018 Mar 5;13(3):e0193409. doi: 10.1371/journal.pone.0193409. eCollection 2018.
