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Laser Microdissection Publication List

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze regions of interest down to single cells to obtain results that are reproducible, and specific. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis. This references list is monthly updated and presents current papers in the major application fields for laser microdissection.

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Topics & Tags

Table of Content

DNA Research

Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens
Su Y, Bidlingmaier S, Lee NK, Liu B
Methods Mol Biol. 2018;1701:331-347. doi: 10.1007/978-1-4939-7447-4_18.

Extrauterine high-grade serous carcinomas with bilateral adnexal involvement as the only two disease sites are clonal based on tp53 sequencing results: implications for biology, classification, and staging
Singh N, Faruqi A, Kommoss F, McCluggage WG, Trevisan G, Senz J, Lum A, Gilks CB, Anglesio M
Mod Pathol. 2017 Nov 17. doi: 10.1038/modpathol.2017.159.

Anti-aminoacyl-tRNA synthetase-related myositis and dermatomyositis: clues for differential diagnosis on muscle biopsy
Cerbelli B, Pisano A, Colafrancesco S, Pignataro MG, Biffoni M, Berni S, De Luca A, Riccieri V, Priori R, Valesini G, d'Amati G, Giordano C
Virchows Arch. 2017 Nov 16. doi: 10.1007/s00428-017-2269-x.

RNA Research

LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
Nichterwitz S, Benitez JA, Hoogstraaten R, Deng Q, Hedlund E
Methods Mol Biol. 2018;1649:95-110. doi: 10.1007/978-1-4939-7213-5_6.

Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis
Zhang S, Thakare D, Yadegari R
Methods Mol Biol. 2018;1676:153-163. doi: 10.1007/978-1-4939-7315-6_9.

Opposite effects of Activin type 2 receptor ligands on cardiomyocyte proliferation during development and repair
Dogra D, Ahuja S, Kim HT, Rasouli SJ, Stainier DYR, Reischauer S
Nat Commun. 2017 Dec 1;8(1):1902. doi: 10.1038/s41467-017-01950-1

Proteomics

Single Cell Immuno-laser Microdissection Coupled to Label-free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia
García-Berrocoso T, Llombart V, Colàs-Campàs L, Hainard A, Licker V, Penalba A, Ramiro L, Simats A, Bustamante A, Martínez-Saez E, Canals F, Sanchez JC, Montaner J
Mol Cell Proteomics. 2017 Nov 13. pii: mcp.RA117.000419. doi: 10.1074/mcp.RA117.000419.

Valeur prédictive du récepteur NKp30 dans la réponse à l’imatinib mesylate des tumeurs stromales gastrointestinales et identification d’un nouveau mécanisme inhibiteur des cellules Natural Killer par la voie TNFα/TNFR2/BIRC3/TRAF1
Ivagnes A
Thesis (article mainly in French), Oncoimmunology

Gastrin Induces Nuclear Export and Proteasome Degradation of Menin in Enteric Glial Cells
Sundaresan S, Meininger CA, Kang AJ, Photenhauer AL, Hayes MM, Sahoo N, Grembecka J, Cierpicki T, Ding L, Giordano TJ, Else T, Madrigal DJ, Low MJ, Campbell F, Baker A-M, Xu H, Wright NA, Merchant JLGastroenterology (2017), doi: 10.1053/j.gastro.2017.08.038.

Fluorescence Applications

Photoreceptor glucose metabolism determines normal retinal vascular growth
Fu Z, Löfqvist CA, Liegl R, Wang Z, Sun Y, Gong Y, Liu CH, Meng SS, Burnim SB, Arellano I, Chouinard MT, Duran R, Poblete A, Cho SS, Akula JD, Kinter M, Ley D, Pupp IH, Talukdar S, Hellström A, Smith LE
EMBO Mol Med. 2017 Nov 27. pii: e201707966. doi: 10.15252/emmm.201707966.

Single Cell Immuno-laser Microdissection Coupled to Label-free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia
García-Berrocoso T, Llombart V, Colàs-Campàs L, Hainard A, Licker V, Penalba A, Ramiro L, Simats A, Bustamante A, Martínez-Saez E, Canals F, Sanchez JC, Montaner J
Mol Cell Proteomics. 2017 Nov 13. pii: mcp.RA117.000419. doi: 10.1074/mcp.RA117.000419.

Gastrin Induces Nuclear Export and Proteasome Degradation of Menin in Enteric Glial Cells
Sundaresan S, Meininger CA, Kang AJ, Photenhauer AL, Hayes MM, Sahoo N, Grembecka J, Cierpicki T, Ding L, Giordano TJ, Else T, Madrigal DJ, Low MJ, Campbell F, Baker A-M, Xu H, Wright NA, Merchant JL
Gastroenterology (2017), doi: 10.1053/j.gastro.2017.08.038.

Cancer Research

Extrauterine high-grade serous carcinomas with bilateral adnexal involvement as the only two disease sites are clonal based on tp53 sequencing results: implications for biology, classification, and staging
Singh N, Faruqi A, Kommoss F, McCluggage WG, Trevisan G, Senz J, Lum A, Gilks CB, Anglesio M
Mod Pathol. 2017 Nov 17. doi: 10.1038/modpathol.2017.159.

A tissue microRNA signature that predicts the prognosis of breast cancer in young women
Hironaka-Mitsuhashi A, Matsuzaki J, Takahashi RU, Yoshida M, Nezu Y, Yamamoto Y, Shiino S, Kinoshita T, Ushijima T, Hiraoka N, Shimizu C, Tamura K, Ochiya T
PLoS One. 2017 Nov 15;12(11):e0187638. doi: 10.1371/journal.pone.0187638. eCollection 2017.

Differential preventive activity of sulindac and atorvastatin in Apc+/Min-FCCCmice with or without colorectal adenomas
Chang W-C, Jackson C, Riel S, Cooper HS, Devarajan K, Hensley HH, Zhou Y, Vanderveer LA, Nguyen MT, Clapper ML
Gut 2017;0:1–9. doi:10.1136/gutjnl-2017-313942

Neurosciences

Intranasally administered IGF-1 inhibits spreading depression in vivo
Grinberg YY1, Zitzow LA2, Kraig RP
Brain Res. 2017 Dec 15;1677:47-57. doi: 10.1016/j.brainres.2017.09.022. Epub 2017 Sep 23.

Astrocyte reactivity and astrogliosis after spinal cord injury
Okada S, Hara M, Kobayakawa K, Matsumoto Y, Nakashima Y
Neurosci Res. 2017 Oct 17. pii: S0168-0102(17)30592-8. doi: 10.1016/j.neures.2017.10.004. Review.

Laser-captured microglia in the Alzheimer’s and Parkinson’s brain reveal unique regional expression profiles and suggest a potential role for hepatitis B in Alzheimer’s brain
Mastroeni D, Nolz J,Sekar S, Delvaux E, Serrano G, Cuyugan L, Liang WS, Beach TG, Rogers J, Coleman PD
Neurobiology of Aging, 2017, ISSN 0197-4580, doi.org/10.1016/j.neurobiolaging.2017.10.019.

Clinical Applications

Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens
Su Y, Bidlingmaier S, Lee NK, Liu B
Methods Mol Biol. 2018;1701:331-347. doi: 10.1007/978-1-4939-7447-4_18.

miR-365 promotes diabetic retinopathy through inhibiting Timp3 and increasing oxidative stress
Wang J, Zhang, J, Chen X, Yang Y, Wang F, Li W, Awuti M, Sun Y, Lian C, Li Z, Wang M, Xu J-Y, Jin C, Tian H, Gao F, Zhang J, Sinha D, Lu L, Xu G-T
Experimental Eye Research, 0014-4835, 28 Nov 2017, doi.org/10.1016/j.exer.2017.11.006

Anti-aminoacyl-tRNA synthetase-related myositis and dermatomyositis: clues for differential diagnosis on muscle biopsy
Cerbelli B, Pisano A, Colafrancesco S, Pignataro MG, Biffoni M, Berni S, De Luca A, Riccieri V, Priori R, Valesini G, d'Amati G, Giordano C
Virchows Arch. 2017 Nov 16. doi: 10.1007/s00428-017-2269-x.

Developmental Research, Epigenetics & Aging

Regulation of mesenchymal signaling in palatal mucosa differentiation
Neupane S, Adhikari N, Jung JK, An CH, Lee S, Jun JH, Kim JY, Lee Y, Sohn WJ, Kim JY
Histochem Cell Biol. 2017 Dec 5. doi: 10.1007/s00418-017-1620-2.

Opposite effects of Activin type 2 receptor ligands on cardiomyocyte proliferation during development and repair
Dogra D, Ahuja S, Kim HT, Rasouli SJ, Stainier DYR, Reischauer S
Nat Commun. 2017 Dec 1;8(1):1902. doi: 10.1038/s41467-017-01950-1

Photoreceptor glucose metabolism determines normal retinal vascular growth
Fu Z, Löfqvist CA, Liegl R, Wang Z, Sun Y, Gong Y, Liu CH, Meng SS, Burnim SB, Arellano I, Chouinard MT, Duran R, Poblete A, Cho SS, Akula JD, Kinter M, Ley D, Pupp IH, Talukdar S, Hellström A, Smith LE
EMBO Mol Med. 2017 Nov 27. pii: e201707966. doi: 10.15252/emmm.201707966.

Plant Research

Laser-Capture Microdissection of Maize Kernel Compartments for RNA-Seq-Based Expression Analysis
Zhang S, Thakare D, Yadegari R
Methods Mol Biol. 2018;1676:153-163. doi: 10.1007/978-1-4939-7315-6_9.

Sequential gene activation and gene imprinting during early embryo development in maize
Meng D, Zhao J, Zhao C, Luo H, Xie M, Liu R, Lai J, Zhang X, Jin W
Plant J. 2017 Nov 24. doi: 10.1111/tpj.13786.

An uncanonical CCCH-tandem zinc finger protein represses secondary wall synthesis and controls mechanical strength in rice
Zhang D, Xu, Z, Cao S, Chen K, Li S, Liu X, Gao X, Zhang B, Zhou Y
Molecular Plant, 1674-2052, doi.org/10.1016/j.molp.2017.11.004

Forensics

Establishing the limit of detection of massively parallel sequencing using laser micro-dissected cells
Nancollis G, England R, Harbison SA
Forensic Science International: Genetics Supplement Series, Sep 2017

Forensic Biology – Expert Evidence
Gunn P, Roebuck H, Summerell A
Bookchapter, reviewed March 2017

Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process
Hansson O, Egeland T, Gill P
Int J Legal Med. 2016 Nov 3

Information for practitioners
Helpful information, videos, and many tipps & tricks on laser microdissection in forensic applications can be found here.

Miscellaneous

Metabolic versatility of a novel N2-fixing Alphaproteobacterium isolated from a marine oxygen minimum zone
Martínez-Pérez C, Mohr W, Schwedt A, Dürschlag J, Callbeck CM, Schunck H, Dekaezemacker J, Buckner CRT, Lavik G, Fuchs BM, Kuypers MMM:
Environ Microbiol. 2017 Dec 1. doi: 10.1111/1462-2920.14008.

Aβ oligomer eliminating compounds interfere successfully with pEAβ (3–42) induced motor neurodegenerative phenotype in transgenic mice
Dunkelmann T, Teichmann K, Ziehm T, Schemmert S, Frenzel D, Tusche M, Dammers C, Jürgens D, Langen K-J, Demuth H-U, Shah NJ, Kutzsche J, Willuweit A, Willbold D
Neuropeptides, 0143-4179, 27 Nov 2017, doi.org/10.1016/j.npep.2017.11.011

Interrogating marine virus-host interactions and elemental transfer with BONCAT and nanoSIMS-based methods
Pasulka AL, Thamatrakoln K, Kopf SH, Guan Y, Poulos B, Moradian A, Sweredoski MJ, Hess S, Sullivan MB, Bidle KD, Orphan VJ
Environ Microbiol. 2017 Nov 21. doi: 10.1111/1462-2920.13996.

Live Cell Culture

miR-182 Regulates Slit2-Mediated Axon Guidance by Modulating the Local Translation of a Specific mRNA
Bellon A, Iyer A, Bridi S, Lee FC, Ovando-Vázquez C, Corradi E, Longhi S, Roccuzzo M, Strohbuecker S, Naik S, Sarkies P, Miska E, Abreu-Goodger C, Holt CE, Baudet ML
Cell Rep. 2017 Jan 31;18(5):1171-1186. doi: 10.1016/j.celrep.2016.12.093.

Identification of cardiac progenitors that survive in the ischemic human heart after ventricular myocyte death
Omatsu-Kanbe M, Nozuchi N, Nishino Y, Mukaisho KI, Sugihara H, Matsuura H
Sci Rep. 2017 Jan 25;7:41318. doi: 10.1038/srep41318.

Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase
Lee KJ, Saha J, Sun J, Fattah KR, Wang SC, Jakob B, Chi L, Wang SY, Taucher-Scholz G, Davis AJ, Chen DJ
Nucleic Acids Res. 2016 Feb 29;44(4):1732-45. doi: 10.1093/nar/gkv1499.