Made entirely out of glass, the NeuroExaminer offers significant advantages over other devices conventionally made from polydimethylsiloxane (PDMS) that exhibit autofluorescence and tend to absorb small molecules. Although the NeuroExaminer was developed around the Leica digital light sheet (DLS) microscope, its design is thought to be compatible with a variety of selective plane illumination (SPIM) or oblique plane microscopy techniques. Furthermore, gentle manipulation allows to image individual larvae at multiple time points and thereby to observe changes in brain activity not only on short (seconds to minutes) but also on longer (hours to days) time scales. In the future, automatic loading and targeted stimuli application together with whole-brain in vivo imaging will provide a unique opportunity to gain novel insights into brain function in health and disease, and may ultimately also contribute to the development of novel and better treatments of neuropsychiatric disorders such as anxiety, depression, and drug addiction.
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