Hernandez-Clavijo et. al. investigated the importance of TMEM16A, a calcium-activated chloride channel, for axonal pathfinding between the VNO and AOB and its effect on VSN density within the VNO. Whole VNOs and parts of the brain containing the AOB were cleared using CLARITY and imaged with a Leica digital light-sheet microscope. While a TMEM16A-ko influenced the electrical activity of VSNs, no effect was observed on VSN density or axonal pathfinding in the investigated VSN population.
Hernandez-Clavijo A, Sarno N, Gonzalez-Velandia KY, Degen R, Fleck D, Rock JR, Spehr M, Menini A and Pifferi S:
TMEM16A and TMEM16B Modulate Pheromone-Evoked Action Potential Firing in Mouse Vomeronasal Sensory Neurons
eNeuro 25 August 2021, 8 (5) ENEURO.0179-21.2021; DOI: https://doi.org/10.1523/ENEURO.0179-21.2021