Open Multiplexing: Transforming Tissue Research with Cell DIVE

Learn how multiplex imaging with Cell DIVE’s open platform can adapt to meet the needs of your study

Pancreatic Ductal Adenocarcinoma with 11 cancer immunity and progression biomarkers shown –CD3, CD4, CD20, CD31, CD45, Collagen, HLA1, NaKATPase, PCK26, SMA1, and Vimentin. Pancreatic_Ductal_Adenocarcinoma_11_biomarkers_shown.jpg

About this webinar

Cancer is a complex disease at the level of both cells and tissues, and uncovering novel treatments requires understanding the biology and organization of many different cell types and tissues. The ability to properly characterize these different features is limited by the analysis method; genomics methods are incredibly broad, but lack spatial information, and imaging methods frequently lack the breadth to understand multiple interlinked oncological phenomena.

Here, we present Cell DIVE, a complete open multiplex imaging workflow that allows for the imaging of over sixty biomarkers in a single tissue section through iterative staining and dye inactivation. This allows for multiple areas of cancer biology to be explored simultaneously. First, using Cell DIVE, we show how cancer cells in hypoxic regions of pancreatic tumors can adapt to extreme conditions, evade cell death, and avoid immune surveillance. 

Second, we explore how Cell DIVE’s flexible and scalable workflow can grow to meet the needs of your multiplex imaging study while allowing the researcher flexibility in how the workflow is performed. 

Last, we demonstrate how automation can be leveraged to improve the speed of complex imaging studies and minimize touch points. By utilizing more biomarkers in an imaging study, the influence and relationships of additional biological pathways on cancer progression can be more easily studied, potentially paving the way for better targeting of emerging therapies.

What to expect in the webinar

Key Learnings

  • Learn how crystal-clear whole tissue images enable you to deepen your understanding of the tissue microenvironment
  • Understand how open multiplexing can help you adapt to ever-changing study requirements
  • Watch how open multiplexing lets your research dictate the level of automation required, which antibodies to use, how to build your antibody panel and more

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The Speakers

Katie White, M.Sc.

Product Manager for the Cell DIVE Multiplexed Imaging Solution, Leica Microsystems

Katie White studied Mechanical Engineering as an undergraduate at the University of Delaware and completed a Master’s degree in Biomedical Engineering at the University of New South Wales in Sydney, Australia. Katie began working with microscopes in 2008 as an Field Application Scientist for a high-resolution widefield microscopy system. Since then, she has spent time as a sales specialist and global product support specialist for high-resolution, super-resolution, and high content imaging systems. Now, Katie is the Product Manager for the Cell DIVE Multiplexed Imaging Solution.

Michael Smith, Ph.D.

Application Manager for the Cell DIVE Multiplexed Imaging Solution, Leica Microsystems

Michael completed a Ph.D. in the genetics of DNA repair at Columbia University and a postdoctoral fellowship in genome integrity and cellular aging at Memorial Sloan Kettering Cancer Center, where he leveraged microscopy to understand the real-time dynamics of DNA homeostasis. His passion for answering biological questions using imaging technologies led him to his current position, where he provides technical expertise and customer support for the Cell DIVE platform.

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