Micro-Computed Tomography (micro-CT) of Insect Brain Protocol
Application Note for Leica EM CPD300 - Life Science Research
Species: Blow fly (Lucilia caesar)
Critical point drying of the blow fly with subsequent X-ray micro-computed tomography (micro-CT) to detect neuroanatomical features.
Samples were placed individually in the chambers of Arthropoda holder.
Fixation and Dehydration
|0.1 M phosphate buffer (1.8% Sucrose, pH 7.2)||3x10 min.|
|Ethanol series: 60%, 70%, 80%, 90%, 96%, 100%||2x10 min.|
|Iodine staining (1% iodine solution in 100% ethanol)||overnight|
|Ethanol: 100%||2x10 min.|