Leica EM CPD300 auto Program:
Mounting and Scanning
Dried sample was glued on an insect pin and scanned with an Xradia MicroXCT-200 X-ray imaging system (Carl Zeiss X-ray Microscopy Inc., Pleasanton, USA).
Micro-CT of Insect Larva Protocol
Application Note for Leica EM CPD300 - Life Science Research
Introduction
Species: red blood worm (midge larva)
Critical point drying of midge larvae with subsequent X-ray micro-computed tomography (micro-CT) to reconstruct the inner anatomy.
Procedure
Sample Holder:
Sample was placed individually in the chambers of Arthropoda holder.
Fixation and Dehydration
| 2.5 % Glutardialdehyde (in 0.1 M phosphate buffer) | overnight |
| 0.1 M phosphate buffer (1.8% Sucrose, pH 7.2) | 3x10 min. |
| Ethanol series: 60%, 70%, 80%, 90%, 96%, 100% | 2x10 min. |
| Iodine staining (1% iodine solution in 100% ethanol) | overnight |
| Ethanol: 100% | 2x10 min. |
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