Mounting and Scanning
Dried sample was glued on an insect pin and scanned with an Xradia MicroXCT-200 X-ray imaging system (Carl Zeiss X-ray Microscopy Inc., Pleasanton, USA).
Species: red blood worm (midge larva)
Critical point drying of midge larvae with subsequent X-ray micro-computed tomography (micro-CT) to reconstruct the inner anatomy.
Sample was placed individually in the chambers of Arthropoda holder.
Fixation and Dehydration
|2.5 % Glutardialdehyde (in 0.1 M phosphate buffer)||overnight|
|0.1 M phosphate buffer (1.8% Sucrose, pH 7.2)||3x10 min.|
|Ethanol series: 60%, 70%, 80%, 90%, 96%, 100%||2x10 min.|
|Iodine staining (1% iodine solution in 100% ethanol)||overnight|
|Ethanol: 100%||2x10 min.|