Mitochondrial dynamics quantitatively revealed by STED nanoscopy


Mitochondria are vital subcellular organelles which can generate energy to support the entire life cycle of biological cells. However, conventional fluorescence microscopy cannot provide a high enough resolution to visualize mitochondrial cristae. This study demonstrates the emerging capability of optical STED nanoscopy to investigate intracellular physiological processes with nanoscale resolution for an extended period of time.

Here, the authors developed an enhanced squaraine variant dye (MitoESq-635) to study the dynamic structures of mitochondrial cristae in live cells with a super-resolution technique. They performed time-lapse imaging of the mitochondrial inner membrane in living HeLa cells for over 50 min (3.9 sec per frame with 71.5 sec dark recovery) with a resolution of 35.2 nm. The forms and shapes of the cristae during mitochondrial fusion and fission can be clearly observed. The labelling specificity and performance of MitoESq-635 with low-power STED make it highly attractive as a next-generation standard for long-term super-resolution imaging of mitochondria.

Read the full article:

Yang X., Yang Z., Wu Z., He Y., ShanC., Chai P., Ma C., Tian M., Teng J., Jin D., Yan W., Das P., Qu J. & Xi P.:

Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe

Nature Communications volume 11, Article number: 3699 (2020)

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?

  • Leica Microsystems Inc.
    1700 Leider Lane
    Buffalo Grove, IL 60089 United States
    Office Phone : +1 800 248 0123
    Service Phone : 1 800 248 0223
    Fax : +1 847-236-3009

You will find a more detailed list of local contacts here.