Multicellular Organization during Stem Cell Dynamics

TIRF microscopy used to assess focal adhesions

TIRF microscopy, focal adhesions, Plexin-B2 mutant hNPCs  Focal_adhesions_in_Plexin-B2_mutant_hNPCs_teaser.jpg

Multicellular organization, e.g. during tissue morphogenesis or of human embryonic stem cells, relies on collective cytoskeletal dynamics. During their studies of multicellular developing human embryonic stem cells and neuroprogenitor cells, Alves et al. discovered that Plexin-B2 is an organizer of the actomyosin network and adhesion complexes. They used TIRF (Total Internal Reflection Fluorescence) microscopy to decipher the number, size, and shape of focal adhesions in wildtype and mutant (Plexin-B2 lacking) cells. In TIRF microscopy only a small volume of fluorophores near the cover glass is excited, resulting in a higher signal-to-background ratio. As a result, TIRF microscopy is well suited to study processes like cell-cell or cell-matrix adhesion.

Read the full article:

Junqueira Alves, C., Dariolli, R., Haydak, J. et al.:

Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion.

Nat. Commun. (2021) vol. 12, pp. 6019

DOI: 10.1038/s41467-021-26296-7

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