Abstract

Multiple Microscopy Modes in a Single Sweep with Supercontinuum White Light

The white light laser source, merged with hybrid detectors in photon counting mode and true spectral detection offers a series of different imaging modes from a single instrument. The continuously tunable excitation, combined with different pulse-frequencies covers all requirements for FLIM. Gated detection allows to separate fluorescent species that exert identical emission color, but differ in lifetime. One example is separation of stain-emission from autogenic fluorescence. But also standard confocal imaging benefits from the flexibility in excitation and sensitivity and low dark signal in detection.

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Kuschel LR and Borlinghaus RT:
Advanced Microscopy/Supercontinuum White Light: Multiple microscopy modes in a single sweep with supercontinuum white light

BioOptics World 3 (2013)

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