Multiple Microscopy Modes in a Single Sweep with Supercontinuum White Light
The white light laser source, merged with hybrid detectors in photon counting mode and true spectral detection offers a series of different imaging modes from a single instrument. The continuously tunable excitation, combined with different pulse-frequencies covers all requirements for FLIM. Gated detection allows to separate fluorescent species that exert identical emission color, but differ in lifetime. One example is separation of stain-emission from autogenic fluorescence. But also standard confocal imaging benefits from the flexibility in excitation and sensitivity and low dark signal in detection.