Introduction
Species: Eubostrichus dianae
Critical point drying of nematode Eubostrichus dianae to detect the ectosymbiotic bacteria layer with subsequent gold coating and SEM analysis.
Procedure
Sample Holder:
Samples were placed into the filter discs and porous pots holder.
Fixation and Dehydration
| 2.5% Glutaraldehyde in 0.1M Cacodylate Buffer | 2 h |
| 0.1M Cacodylate Buffer | 3x10 min. |
| 1% OsO4 in 0.1M Cacodylate Buffer | 4-12 h |
| 0.1M Cacodylate Buffer | 3x10 min. |
| Ethanol series: 30%, 50%, 70%, 80%, 80%, 90%, 90%, 100%, 100% | 10 min. |
| 1:1 Mix Ethanol / Acetone | 10 min. |
| 100% Acetone | 10 min. |
Leica EM CPD300 Critical Point Dryer auto Program:
Coating:
Gold: 10-20 nm
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