Fixation and Dehydration
2.5% Glutaraldehyde in 0.1M Cacodylate Buffer | 2 h |
0.1M Cacodylate Buffer | 3x10 min. |
1% OsO4 in 0.1M Cacodylate Buffer | 4-12 h |
0.1M Cacodylate Buffer | 3x10 min. |
Ethanol series: 30%, 50%, 70%, 80%, 80%, 90%, 90%, 100%, 100% | 10 min. |
1:1 Mix Ethanol / Acetone | 10 min. |
100% Acetone | 10 min. |
Leica EM CPD300 Critical Point Dryer auto Program:


Coating:
Gold: 10-20 nm