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Abstract

Observing the effect on transcription factor signaling and chromatin landscapes in cells infected with the human coronavirus 229E

Viral infection triggers an inflammatory cellular response which is characterized by altered gene expression. In this study, the influence of coronaviruses on cellular NF-κB (transcription factor) signaling and the chromatin landscape was investigated. With the help of a LMD6000 laser microdissection system, cells expressing the coronavirus N protein were isolated and their total RNA extracted for downstream analysis. Subsequent quantitative mRNA expression analysis (RT-qPCR) and microarray analysis revealed that the virus has an influence on multiple signaling, immunoregulatory, and metabolic processes. Indirect immunofluorescence analyses were performed with a DM IRE2 or DMi8 fluorescence microscope. Refer to the publication for more details.

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M. Poppe, S. Wittig, L. Jurida, M. Bartkuhn, J. Wilhelm, H. Müller, K. Beuerlein, N. Karl, S. Bhuju, J. Ziebuhr, M. Lienhard Schmitz, M. Kracht:

The NF-κB-dependent and -independent transcriptome and chromatin landscapes of human coronavirus 229E-infected cells

PLOS Pathogens (2017) vol. 13, iss. 3, e1006286

DOI: 10.1371/journal.ppat.1006286