Viral infection triggers an inflammatory cellular response which is characterized by altered gene expression. In this study, the influence of coronaviruses on cellular NF-κB (transcription factor) signaling and the chromatin landscape was investigated. With the help of a LMD6000 laser microdissection system, cells expressing the coronavirus N protein were isolated and their total RNA extracted for downstream analysis. Subsequent quantitative mRNA expression analysis (RT-qPCR) and microarray analysis revealed that the virus has an influence on multiple signaling, immunoregulatory, and metabolic processes. Indirect immunofluorescence analyses were performed with a DM IRE2 or DMi8 fluorescence microscope. Refer to the publication for more details.
PLOS Pathogens (2017) vol. 13, iss. 3, e1006286
DOI: 10.1371/journal.ppat.1006286
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