Key publications of the Nobel Prize for Chemistry 2014
- Betzig E:
Proposed method for molecular optical imaging. Opt. Lett. 20: 237–39 (1995). - Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, and Hess HF:
Imaging intracellular fluorescent proteins at nanometer resolution. Science 313: 1642–45 (2006). - Dickson RM, Cubitt AB, Tsien RY, and Moerner WE:
On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature 388: 355–58 (1997). - Hell SW and Wichman J:
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion-microscopy. Opt. Lett. 19: 780–82 (1994). - Hell SW and Kroug M:
Ground-state depletion fluorescence microscopy, a concept for breaking the diffraction resolution limit. Appl. Phys. B. 60: 495–97 (1995). - Klar TA, Jakobs S, Dyba M, Egner A, and Hell SW:
Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proc. Natl. Acad. Sci. USA 97: 8206–10 (2000). - Moerner WE and Kador L:
Optical detection and spectroscopy of single molecules in a solid. Phys. Rev. Lett. 62: 2535–38 (1989).
Articles on Science Lab related to Stefan Hell
- Hell S, Schué A, and Köster I:
Video Interview with Stefan Hell, the Inventor of Super-Resolution (2014) - Hell S:
Video Talk on Super-Resolution: Overview and Stimulated Emission Depletion (STED) Microscopy (2014) - Andersen P:
Interview with Stefan Hell: Breaking Nanoscale Boundaries (2013) - Freebody M:
STED Microscopy: A New Chapter in Light Imaging (2013) - Turku BioImaging:
Video: STED Microscopy at Turku BioImaging (2013) - Schauen M, Opazo F, and Rizzoli S:
New Labeling Tools Can Help to Realize the Full Potential of Super-Resolution Microscopy (2013) - Borlinghaus RT:
Super-resolution Microscopy and the Third Dimension (2013) - Schauen M, and Kukat C:
Mitochondrial DNA Molecules are Packaged Individually (2013) - Berning S, Willig K, Steffens H, Dibaj P, and Hell S:
Sharp Live Images from the Mouse Brain (2012) - Brismar H, Hell S, Eggeling C, Szymborska A, Diaspro A, Hosy E, Vicidomini G, and van der Voort H:
Abstracts of the 2nd European Super-Resolution User-Club Meeting (2012) - Sigrist S, Diaspro A, Rizzoli S, Eggeling C, Nägerl V, and Willig K:
Abstracts of the First European Super-Resolution User Club Meeting (2011) - Borlinghaus RT:
Unlimited Resolution – STED (2011) - Borlinghaus RT:
Neuroscience and Microscopy – A Rewarding Partnership (2011) - Videos on STED Microscopy and its Inventor Stefan Hell (2011):
- Stefan Hell and the story on STED (2011 Körber European Science Prize)
- Nanoscopy with Focused Light – Stefan Hell presentation from SPIE Photonics West 2011
- Stefan Hell on nanometer-scale microscopy in biophotonics
- Better Resolution for Microscopes Discovered (Hansen Familiy Award)
- Hell S, Shroff H, and Selvin PR:
Webinar: Super-Resolution – Light Microscopy for the 21st Century (2011) - Perkel J:
A STED-y Route to Commercialization (2011) - Schué A, Sigrist S, Rizzoli S, and Wilczynski G:
Observing Life’s Nanostructures with STED (2009) - Interview with Stefan Hell – For me, Pioneering is ... (2008)
STED key publications
- First publication on STED technology:
Hell SW and Wichmann J: Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics letters 19: 780–82 (1994). - First biological image:
Klar TA, Jakobs S, Dyba M, Egner A, and Hell SW: Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proceedings of the National Academy of Sciences of the United States of America 97: 8206–10 (2000). - STED with genetically encoded marker: GFP
Willig KI et al.: Nanoscale resolution in GFP-based microscopy. Nature methods 3: 721–23 (2006). - STED with continuous wave laser beams:
Willig KI, Harke B, Medda R, and Hell SW: STED microscopy with continuous wave beams. Nature methods 4: 915–18 (2007). - Two-color STED with two STED lines:
Donnert G et al.: Two-color far-field fluorescence nanoscopy. Biophysical journal 92: L67–69 (2007). - Iso-STED and two-color STED with one STED line:
Schmidt R. et al.: Spherical nanosized focal spot unravels the interior of cells. Nature methods 5: 539–44 (2008). - STED- FCS application:
Eggeling C. et al.: Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature 457: 1159–62 (2009). - Gated STED:
Vicidomini G et al.: Sharper low-power STED nanoscopy by time gating. Nature methods 8: 571–73 (2011).
GSD key publications
- First publication on GSD technology:
Hell SW and Kroug M: Ground-state-depletion fluorscence microscopy: A concept for breaking the diffraction resolution limit. Applied Physics B Lasers and Optics 60 (5): 495–97 (1995). doi: 10.1007/BF01081333.
- First biological image (home built microscope, scanner based, named GSD microscopy):
Bretschneider S, Eggeling C and Hell SW: Breaking the diffraction barrier in fluorescence microscopy by optical shelving. Physical Review Letters 98 (21): 218103 (2007)
- First biological image (home built microscope, camera based, named GSDIM microscopy):
Fölling J, Bossi M, Bock H, Medda R, Wurm CA, Hein B, Jakobs S, Eggeling C and Hell SW: Fluorescence nanoscopy by ground-state depletion and single-molecule return. Nature Methods 5 (11): 943–45 (2008). doi: 10.1038/nmeth.1257. - Multicolor GSDIM in fixed and living cells:
Testa I, Wurm CA, Medda R, Rothermel E, von Middendorf C, Fölling J, Jakobs S, Schönle A, Hell S and Eggeling C: Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength. Biophysical Journal 99 (8): 2686–94 (2010). doi: 10.1016/j.bpj.2010.08.012. - GSDIM with Bacteria:
Baig F, Fernando LP, Salazar MA, Powell RR, Bruce TF and Harcum SW: Dynamic transcriptional response of Escherichia coli to inclusion body formation. Biotechnology and Bioengineering (2013). doi: 10.1002/bit.25169. - GSDIM with Viruses:
Weber M, Gawanbacht A, Habjan M, Rang A, Borner C, Schmidt AM, Veitinger S, Jacob R, Devignot S, Kochs G, García-Sastre A, Weber F: Incoming RNA virus nucleocapsids containing a 5’-triphosphorylated genome activate RIG-I and antiviral signaling. Cell Host & Microbe 13 (3): 336–46 (2013). doi: 10.1016/j.chom.2013.01.012
