The Microscopy Knowledge Portal
The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
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Topics – A Vast Amount of Expert Knowledge
- Basics in Microscopy
- Confocal Microscopy
- Digital Microscopy
- Dentistry
- EM Sample Preparation
- Fluorescence Microscopy
- Light Sheet Microscopy
- Neurosurgery
- Optical Coherence Tomography
- Super-Resolution
- Stereo Microscopy
- Surface Metrology
- Quality Assurance
- Otolaryngology (ENT)
- Ophthalmology
- Plastic Reconstructive
- Widefield Microscopy
- More
Latest Articles
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Dissecting proteomic heterogeneity of the tumor microenvironment
This lecture will highlight cutting edge applications in applying laser microdissection and microscaled quantitative proteomics and phosphoproteomics to uncover exquisite intra- and inter-tumor…Read article
Elevate your inspection workflow for medical devices
This webinar will demonstrate a smart device for traceable microscopy that helps you achieve more consistent visual inspection of medical devices across multiple users. Additionally, this device…Read article
Developmental Biology Image Gallery
Developmental biology explores the development of complex organisms from the embryo to adulthood to understand in detail the origins of disease. This category of the gallery shows images about…Read article
Going Digital for Medical Device Visual Inspection
This article discusses the reasons why paper-based documentation and reporting makes visual microscope inspection of medical devices inefficient and unreliable. It also addresses other factors…Read article
Kinetochore assembly during mitosis with TauSTED on 3D
Three-dimensional organization of the mitotic spindle together with the distribution of CENP-C and BUB1 based on TauSTED with multiple STED lines (592, 660 and 775 nm) can provide insights on…Read article
Fast 2 colour live cell dynamics with TauSTED gentle super-resolution
TauSTED, with low photon budget and the fast detection scheme in our STELLARIS confocal platform, enables the tracking of highly dynamic processes in live cells. We can see in this example the…Read article
“Brains-In-A-Dish” from Induced Pluripotent Stem Cells (iPSCs)
This article discusses the benefits of using the THUNDER technology for imaging inside 3D human cortical brain organoids. These organoids are derived from human induced pluripotent stem cells (iPSCs)…Read article
Fission Yeast Actomyosin Ring Integrity
This article demonstrates that the stress-activated protein kinase pathway (SAPK) and its effector, MAPK Sty1, downregulates CAR assembly in the fission yeast Schizosaccharomyces pombe when its…Read article
How to Quantify Changes in the Metabolic Status of Single Cells
Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed. Now,…Read article
Developing Heart Pacemaker Cells from Cardiac Spheroids
During the last decade, 3D cell culture has been established as a more realistic model compared to classical 2D culture systems. Cells can develop into miniature 3D objects, so called spheroids, which…Read article
Putting Dynamic Live Cell Data into the Ultrastructural Context
With the Leica Nano Workflow, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…Read article
Into the Third Dimension with "Wow Effect"- Observe Cells in 3D and Real-Time
Life is fast, especially for a cell. As a rule, cells should be examined under physiological conditions which are as close as possible to their natural environment. New technologies offer tremendous…Read article
Observing 3D Cell Cultures During Development
3D cell cultures, such as organoids and spheroids, give insights into cells and their interactions with their microenvironment. These 3D cell cultures are playing an increasingly important role for…Read article
Label-free Differentiation of Microplastics
Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of pixel-accurate lifetime distributions in a sample…Read article
Cytosolic Calcium Ions in Melanoma Cancer Cells
In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging is presented…Read article
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