Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-illumination using narrow-band blue (490 nm) light. Note the increased image contrast (Ploem, 1967)

Milestones in Incident Light Fluorescence Microscopy

Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…

Acousto Optics in True Confocal Spectral Microscope Systems

Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this…

Handbook of Optical Filters for Fluorescence Microscopy

Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and…