Science Lab
Science Lab
The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
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Pinhole Effect in Confocal Microscopes
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its…
Apr 26, 2017Story - Loading...
Pinhole Geometry: Four Corners are Perfect
Square and hexagonal pinholes provide identical image signal levels, if the geometries are compared in a sensible manner. The amount of light passing…
May 23, 2013Tutorial - Loading...
Pinhole Controls Optical Slicing
True confocal scanning microscopes (TCS) use a variable detection pinhole. Good optical sectioning tries to use just the inner core of the PSF.
Jun 21, 2011Tutorial - Loading...
Confocal Optical Section Thickness
Confocal microscopes are employed to optically slice comparably thick samples.
Jun 20, 2011Tutorial