


The Microscopy Knowledge Portal
The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
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Topics – A Vast Amount of Expert Knowledge
- Basics in Microscopy
- Confocal Microscopy
- Digital Microscopy
- Dentistry
- EM Sample Preparation
- Fluorescence Microscopy
- Light Sheet Microscopy
- Neurosurgery
- Optical Coherence Tomography
- Super-Resolution
- Stereo Microscopy
- Surface Metrology
- Quality Assurance
- Otolaryngology (ENT)
- Ophthalmology
- Plastic Reconstructive
- Widefield Microscopy
- More
Latest Articles
How to improve your Biomarker Discovery Workflow with Laser Microdissection
Biomarkers can be used as indicators of certain diseases, such as cancer. The tumor microenvironment moved into the spotlight in this concern. It is in close interaction with the tumor itself.…Read articleKey Factors to Consider When Selecting a Stereo Microscope
Stereo microscopes are often nicknamed the “workhorse” of the lab or production site. Users spend many hours looking through the eyepieces inspecting, observing, documenting, or dissecting samples.…Read articleIntroduction to Ion Beam Etching with the EM TIC 3X
In this article you can learn how to optimize the preparation quality of your samples by using the ion beam etching method with the EM TIC 3X ion beam milling machine. A short introduction of the…Read articleHow to Drape an Overhead Surgical Microscope
The tutorial features the Leica ARveo digital Augmented Reality microscope for complex neurosurgery. The procedure also applies to the Leica M530 OHX, OH6, OH5 and OH4.Read articleHow to Drape a Surgical Microscope
Before performing surgical procedures, it is important to drape the surgical microscope to ensure sterile working conditions. At Leica, we are committed to helping you with your surgical practice. In…Read articleHow to prepare samples for Stimulated Raman Scattering
Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or…Read articlePrinciples of Multiphoton Microscopy for Deep Tissue Imaging
Basics of multiphoton microscopy. This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy uses excitation wavelengths in the infrared taking…Read articleStep by Step Guide to the Molecular Basics of CARS Microscopy
CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial…Read articleBrief Introduction to Surface Metrology
This report briefly discusses several important metrology techniques and standard definitions commonly used to assess the topography of surfaces, also known as surface texture or surface finish. With…Read articleIntroduction to Ultramicrotomy
When studying samples, to visualize their fine structure with nanometer scale resolution, most often electron microscopy is used. There are 2 types: scanning electron microscopy (SEM) which images the…Read articleThe Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS SP8 STED 3X nanoscope from Leica Microsystems. It…Read articleAutomated topographic analysis with the Leica Map software
Watch this video to learn how you can save time when doing topographic analysis using the Leica Map software. The software allows you to go quickly from automated analysis of multiple sets of acquired…Read articleHow to create EDOF (Extended Depth of Focus) images
Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the optional Extended Depth of Focus (EDOF) function of…Read articleUsing Mounting Frames for DLS Sample Preparation
Sample handling is an important topic in the context of Light Sheet Microscopy. The TCS SP8 DLS integrates Light Sheet technology into an inverted confocal platform and can hence make use of general…Read articleUsing a Rotation Device for DLS Sample Mounting
The TCS SP8 DLS from Leica Microsystems is an innovative concept to integrate the Light Sheet Microscopy technology into the confocal microscope. Due to its unique optical architecture samples can be…Read article