Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Latest Articles

Loading...
Gaining Insights into Colorectal Cancer Metastasis
To follow cancer metastasis at the point of occurrence intravital multiphoton microscopy is the tool of choice. Intravital microscopy allows…
Jun 18, 2021
Abstract
Loading...
Accessing the Metabolic Information of Stem Cells
Cancer often comes with changes in the metabolism of cells. Monitoring the metabolic state using fluorescence lifetime imaging microscopy (FLIM) is a…
Jun 18, 2021
Abstract
Loading...
Kinetochore Assembly during Mitosis with TauSTED on 3D
Three-dimensional organization of the mitotic spindle together with the distribution of CENP-C and BUB1 based on TauSTED with multiple STED lines…
Jun 02, 2021
Abstract
Loading...
Fast 2 Colour Live Cell Dynamics with TauSTED Gentle Super-resolution
TauSTED, with low photon budget and the fast detection scheme in our STELLARIS confocal platform, enables the tracking of highly dynamic processes in…
Jun 02, 2021
Abstract
Loading...
Fission Yeast Actomyosin Ring Integrity
This article demonstrates that the stress-activated protein kinase pathway (SAPK) and its effector, MAPK Sty1, downregulates CAR assembly in the…
May 28, 2021
Abstract
Loading...
Label-free Differentiation of Microplastics
Phasor analysis for fluorescence lifetime imaging microscopy (FLIM) is a powerful and fitting-free tool that provides a 2D graphical assessment of…
May 12, 2021
Abstract
Loading...
Cytosolic Calcium Ions in Melanoma Cancer Cells
In this article, a ratiometric fluorometric method for cytosolic calcium ion (Ca2+) measurement in cultured melanoma cells using Fura 2-AM cell…
May 12, 2021
Abstract
Loading...
Fish and Chips Sheet into the Light
The zebrafish larval brain is small and contains about 1 million times less neurons than the human counterpart. The neural circuitry and the general…
May 05, 2021
Abstract
Loading...
A Quality Metric for the Systematic Evaluation of Clearing Protocols
3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they…
Apr 15, 2021
Abstract
Loading...
How FLIM Microscopy helps to detect microplastic pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not…
Dec 28, 2020
Abstract
Loading...
OCT-Analysis of a Clear Corneal Incision
The aim of this prospective study was to analyse the effect of lateral stromal hydration on the morphology of clear corneal incision architecture…
Sep 22, 2020
Abstract
Loading...
Coronavirus Replication in Host Cells
In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in…
Sep 14, 2020
Abstract
Loading...
IL-18 cytokine derived from the enteric nervous system is important for intestinal immunity
Interleukin 18 (IL-18) is a proinflammatory cytokine, which induces cell-mediated immune reaction upon bacterial infection. In the intestine, it is…
Jul 10, 2020
Abstract
Loading...
Observing the effect on transcription factor signaling and chromatin landscapes in cells infected with the human coronavirus 229E
Viral infection triggers an inflammatory cellular response which is characterized by altered gene expression. In this study, the influence of…
Jun 25, 2020
Abstract
Loading...
Cholesterol Homeostasis Modulates Platinum Sensitivity in Human Ovarian Cancer
Ovarian cancer is one of the most severe types of cancers that women can suffer from during their lifetimes. It is the cancer’s tendency for frequent…
Jun 02, 2020
Abstract
Loading...
New Light Shed on the Nanodomain Organization of the Endoplasmic Reticulum (ER)
The endoplasmic reticulum (ER) is a continuous membrane organelle in charge of protein synthesis, lipid synthesis and detoxification. The ER structure…
Dec 02, 2019
Abstract
Loading...
Medicinal Use of Qianhu Plant Roots
This study focused on tissue-specific metabolite profiling and comparison of various parts of bolting and unbolting Peucedanum praeruptorum Dunn…
Nov 06, 2019
Abstract
Loading...
Pore Scale Visualization of Drainage in 3D Porous Media by Confocal Microscopy
This work reports new interesting findings concerning porous media infiltration which have direct implications for the fields of energy (oil recovery)…
Oct 28, 2019
Abstract