Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Latest Articles

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Visualizing Tropoelastin in a Long-Term Human Elastic Fibre Cell Culture Model
Elastin is an essential protein found in a variety of tissues where resilience and flexibility are needed, such as the skin and the heart. When aiming…
Oct 24, 2016
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Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles
Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic…
Oct 19, 2016
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Clearing of Fixed Tissue: A Review from a Microscopist’s Perspective
Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including…
Sep 12, 2016
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The Apical Ectodermal Ridge of the Mouse Model of Ectrodactyly Dlx5;Dlx6−/− Shows Altered Stratification and Cell Polarity, which are Restored by Exogenous Wnt5a Ligand
The congenital malformation split hand/foot (SHFM) is characterized by missing central fingers and dysmorphology or fusion of the remaining ones.…
Sep 07, 2016
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Adeno-associated Viral Vectors do not Efficiently Target Muscle Satellite Cells
Adeno-associated viral (AAV) vectors are becoming an important tool for gene therapy of numerous genetic and other disorders. Several recombinant AAV…
Sep 05, 2016
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Mirror-Enhanced Super-Resolution Microscopy
Axial excitation confinement beyond the diffraction limit is crucial to the development of next-generation, super-resolution microscopy. STimulated…
Aug 18, 2016
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The Actin Cytoskeleton Modulates the Activation of iNKT Cells by Segregating CD1d Nanoclusters on Antigen-Presenting Cells
The ability of invariant natural killer T (iNKT) cells to recognize endogenous antigens represents a distinct immune recognition strategy, which…
Aug 10, 2016
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Highly Selective Fluorescent and Colorimetric Probe for Live-cell Monitoring of Sulphide Based on Bioorthogonal Reaction
H2S is the third endogenously generated gaseous signaling compound and has also been known to involve a variety of physiological processes. To better…
Jul 29, 2016
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Translation Microscopy (TRAM) for Super-Resolution Imaging
Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the…
Jul 22, 2016
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Label-free in vivo Imaging of Myelinated Axons in Health and Disease with Spectral Confocal Reflectance Microscopy
We report a new technique for high-resolution in vivo imaging of myelinated axons in the brain, spinal cord and peripheral nerve that requires no…
Jul 01, 2016
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Two-Photon Excitation STED Microscopy with Time-Gated Detection
We report on a novel two-photon excitation stimulated emission depletion (2PE-STED) microscope based on time-gated detection. The time-gated detection…
Jun 21, 2016
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Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent
Super-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here,…
Apr 27, 2016
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4Pi-RESOLFT Nanoscopy
Here we apply the 4Pi scheme to RESOLFT nanoscopy using two-photon absorption for the on-switching of fluorescent proteins. We show that in this…
Feb 26, 2016
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Cryo-electron Microscopy of Tubular Arrays of HIV-1 Gag Resolves Structures Essential for Immature Virus Assembly
HIV-1 undergoes a two-step assembly process. First, an immature noninfectious particle is assembled, which leaves the infected cell. Second, the…
Feb 09, 2016
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Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and…
Feb 05, 2016
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How to Combine STED and CLARITY
Previously, the preferred way to study the subtlest elements of the kidney, such as foot processes and the slit diaphragm has been by the use of…
Jan 29, 2016
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A Healthy Dose of Interferometry
Although interferometry may once have been dismissed by the medical community because of its inability to see inside the human body, its application…
Jan 14, 2016
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TEM Sample Preparation Made Easy - Prepare TEM Specimen by Broad Beam Argon Ion Milling
Quantitative and analytical analysis at high spatial resolution places stringent demands on the quality of the produced TEM specimens. Pristine and…
Dec 15, 2015
Abstract