Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Latest Articles

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An Innovative Non-contact Method to Determine Surface Free Energy on Micro-areas

Surface free energy (SFE) is a property which depends on the chemical state and roughness of the surface and it is necessary to develop a reliable…

Apr 28, 2014

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Focus on Microscopy 2014 – Abstracts of the Presentations Online Available

Focus on Microscopy 2014 was held at the University of Sydney, Australia from April 13–16. Over 600 delegates and exhibitors attend this year’s FOM…

Apr 25, 2014

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Surface Roughness of Flowable Resin Composites eroded by Acidic and Alcoholic Drinks

Acidic beverages, such as soft drinks (orange juice, and cola), or ethanol (whisky), can produce erosion of resin composites. The surface degradation…

Apr 24, 2014

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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this…

Mar 28, 2014

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A New Filtering Technique for Removing Anti-Stokes Emission Background in Gated CW-STED Microscopy

Stimulated emission depletion (STED) microscopy is a prominent approach of super-resolution optical microscopy, which allows cellular imaging with so…

Mar 25, 2014

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Genomic Survey, Gene Expression Analysis and Structural Modeling Suggest Diverse Roles of DNA Methyltransferases in Legumes

DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases),…

Mar 14, 2014

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Glucose-Stimulated Calcium Dynamics in Islets of Langerhans in Acute Mouse Pancreas Tissue Slices

In endocrine cells within islets of Langerhans calcium ions couple cell stimulation to hormone secretion. Since the advent of modern fluorimetry,…

Mar 11, 2014

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Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal…

Jan 30, 2014

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Novel Fluorescent Carbonic Nanomaterials for Sensing and Imaging

Small brightly fluorescent carbon nanoparticles have emerged as a new class of materials important for sensing and imaging applications. We analyze…

Jan 16, 2014

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The Relationship Between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices

Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically…

Dec 17, 2013

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Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Mauthner cells (M-cells) are large reticulospinal neurons located in the hindbrain of teleost fish. They are key neurons involved in a characteristic…

Oct 11, 2013

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STED Microscopy: A New Chapter in Light Imaging

When Stefan Hell invented a technique that pushed beyond the diffraction limit of light for the first time, a new field of nanoscale imaging was…

Sep 16, 2013

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From Dynamic Live Cell Imaging to 3D Ultrastructure

To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines…

Aug 28, 2013

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A Precise and Rapid Mapping Protocol for Correlative Light and Electron Microscopy of small invertebrate organisms

CLEM (correlative live cell and electronmicroscopy) seeks to bridge the data acquired with different imaging strategies, typically between light…

Jul 16, 2013

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Automated Digital 3D Topography Measurement with the Leica DCM 3D Dual Core Measuring Microscope

The precise measurement of surface structures and topography is the key thing during the production, control and development in many sectors of…

May 17, 2013

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Immersion Freezing of Suspended Particles and Cells for Cryo-Electron Microscopy

Immersion freezing of thin aqueous specimens is an essential preparation technique for cryo-transmission electron microscopy (cryo-TEM), aiming to…

Apr 12, 2013

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Imaging Enzymes at Work

For the understanding of functions of proteins in biological and pathological processes, reporter molecules such as fluorescent proteins have become…

Apr 05, 2013

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Image Cytometry

Microscopy-based imaging is booming and the need for tools to retrieve quantitative data from images is urgent. This book provides simple but reliable…

Apr 04, 2013

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