Science Lab

To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines…

CLEM (correlative live cell and electronmicroscopy) seeks to bridge the data acquired with different imaging strategies, typically between light…

The precise measurement of surface structures and topography is the key thing during the production, control and development in many sectors of…

Immersion freezing of thin aqueous specimens is an essential preparation technique for cryo-transmission electron microscopy (cryo-TEM), aiming to…

For the understanding of functions of proteins in biological and pathological processes, reporter molecules such as fluorescent proteins have become…

Microscopy-based imaging is booming and the need for tools to retrieve quantitative data from images is urgent. This book provides simple but reliable…

This article gives an overview of two different types of superresolution techniques. Stimulated emission depletion (STED) microscopy is a versatile…

This article summarizes the development and differences in design and functionality of confocal technology as far as spectral properties are…

Modern biomedical research is currently dominated by imaging and measuring with optical microscopes. One branch of the microscopy technology is…

Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under…

Basics, history, and applications of multiphoton microscopy. Honouring Goeppert-Mayer’s prediction of simultaneous two-photon absorption by an atom…

Phasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies.…

This four-part series of articles published in Microscopy and Analysis covers the factors to consider in choosing a camera among CCD, EMCCD, and…

In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular…

To explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell’s team of researchers at the Max Planck…

Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized…

The advent of superresolution microscopy has opened up new research opportunities into dynamic processes at the nanoscale inside living biological…

This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective…