Science Lab

Learn. Share. Contribute. The knowledge portal of Leica Microsystems. Find scientific research and teaching material on the subject of microscopy. The portal supports beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies. Become part of the Science Lab community and share your expertise.

Researchers Insights: Microscopy in Cancer Research

Discover how imaging techniques are driving cancer research forward. In this issue, we present comprehensive multimodal studies using microscopy, as well as new directions in intraoperative cancer…

Predictive Service Prevents Downtime in Ghent

At the VIB BioImaging Core in Ghent, Belgium, researchers depend on Leica’s Stellaris 8 confocal microscope to explore the frontiers of biomedical science. When Leica’s RemoteCare system detected a…

Zebrafish heart, DAPI (nuclei, blue), Tropomyosin (cardiomyocytes, red) and GFP (primordial cardiac layer, green). Courtesy of Anna Jazwinska, University of Fribourg, Switzerland.

A Guide to Fluorescence Microscopy

Fluorescence microscopy uses the ability of fluorophores, dyes, or fluorescent proteins to emit light of a specific wavelength after being excited with light of a shorter wavelength. Biomolecules can…

Some 2D measurements, e.g., lengths and areas, made on a PCB sample with a Leica measurement microscope using the Enersight software.

How to Select the Right Measurement Microscope

With a measurement microscope, users can measure the size and dimensions of sample features in both 2D and 3D, something crucial for inspection, QC, failure analysis, and R&D. However, choosing the…

Example of calibrating a microscope at a higher magnification value using a stage micrometer.

Microscope Calibration for Measurements: Why and How You Should Do It

Microscope calibration ensures accurate and consistent measurements for inspection, quality control (QC), failure analysis, and research and development (R&D). Calibration steps are described in this…

Corneal transplantation. Image courtesy of Mr. David Anderson.

Ophthalmology Case Study: Corneal Transplantation

Learn about the use of intraoperative Optical Coherence Tomography in Corneal Transplantation and how it helps achieve correct positioning of donor tissue.

Optical microscope image, which is a composition of both brightfield and fluorescence illumination, showing organic contamination on a wafer surface. The inset images in the upper left corner show the brightfield image (above) and fluorescence image (below with dark background).

Visualizing Photoresist Residue and Organic Contamination on Wafers

As the scale of integrated circuits (ICs) on semiconductors passes below 10 nm, efficient detection of organic contamination, like photoresist residue, and defects during wafer inspection is becoming…

Image of magnetic steel taken with a 100x objective using Kerr microscopy. The magnetic domains in the grains appear in the image with lighter and darker patterns. A few domains are marked with red arrows. Courtesy of Florian Lang-Melzian, Robert Bosch GmbH, Germany.

Rapidly Visualizing Magnetic Domains in Steel with Kerr Microscopy

The rotation of polarized light after interaction with magnetic domains in a material, known as the Kerr effect, enables the investigation of magnetized samples with Kerr microscopy. It allows rapid…

Region of a patterned wafer inspected using optical microscopy and automated and reproducible DIC (differential interference contrast). With DIC users are able to visualize small height differences on the wafer surface more easily.

6-Inch Wafer Inspection Microscope for Reliably Observing Small Height Differences

A 6-inch wafer inspection microscope with automated and reproducible DIC (differential interference contrast) imaging, no matter the skill level of users, is described in this article. Manufacturing…

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