The Microscopy Knowledge Portal
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The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments.
Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
Latest Articles
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How to use a Surgical Microscope as an Operating Room Nurse
Surgical microscopes play an essential role in the modern microsurgery procedures. It provides the surgeon, assistant and operating room staff with a…
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How to improve your Biomarker Discovery Workflow with Laser Microdissection
Biomarkers can be used as indicators of certain diseases, such as cancer. The tumor microenvironment moved into the spotlight in this concern. It is…
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Introduction to Ion Beam Etching with the EM TIC 3X
In this article you can learn how to optimize the preparation quality of your samples by using the ion beam etching method with the EM TIC 3X ion beam…
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How to Drape an Overhead Surgical Microscope
The tutorial features the Leica ARveo digital Augmented Reality microscope for complex neurosurgery. The procedure also applies to the Leica M530 OHX,…
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How to Drape a Surgical Microscope
Before performing surgical procedures, it is important to drape the surgical microscope to ensure sterile working conditions. At Leica, we are…
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How to prepare samples for Stimulated Raman Scattering
Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows…
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Principles of Multiphoton Microscopy for Deep Tissue Imaging
Basics of multiphoton microscopy. This tutorial explains the principles of multiphoton microscopy for deep tissue imaging. Multiphoton microscopy…
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Step by Step Guide to the Molecular Basics of CARS Microscopy
CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic…
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Brief Introduction to Surface Metrology
This report briefly discusses several important metrology techniques and standard definitions commonly used to assess the topography of surfaces, also…
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Introduction to Ultramicrotomy
When studying samples, to visualize their fine structure with nanometer scale resolution, most often electron microscopy is used. There are 2 types:…
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The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS…
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How to create EDOF (Extended Depth of Focus) images
Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the…
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Automated topographic analysis with the Leica Map software
Watch this video to learn how you can save time when doing topographic analysis using the Leica Map software. The software allows you to go quickly…
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Using Mounting Frames for DLS Sample Preparation
Sample handling is an important topic in the context of Light Sheet Microscopy. The TCS SP8 DLS integrates Light Sheet technology into an inverted…
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Using a Rotation Device for DLS Sample Mounting
The TCS SP8 DLS from Leica Microsystems is an innovative concept to integrate the Light Sheet Microscopy technology into the confocal microscope. Due…
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What Does 30,000:1 Magnification Really Mean?
One important criterion concerning the performance of an optical microscope is magnification. This report will offer digital microscopy users helpful…
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How to do a Proper Cell Culture Quick Check
In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium.…
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Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly…
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- Super-Resolution
- Fluorescence Microscopy
- Live-Cell Imaging
- Light Sheet Microscopy
- Widefield Microscopy
- THUNDER Imaging
- Optical Coherence Tomography
- Surgical Microscopy
- Confocal Microscopy
- Laser Microdissection
- Neuroscience
- Forensics
- Quality Assurance
- Stereo Microscopy
- Digital Microscopy
- Ophthalmology
- Multiphoton Microscopy
- Quantitative Imaging
- Coherent Raman Scattering (CRS)
- CLEM
- EM Sample Preparation
- Neurosurgery
- Education
- Otolaryngology (ENT)
- Plastic Reconstructive
- TIRF Microscopy
- Surface Metrology
- Metallography
- Basics in Microscopy
- Steel Quality
- Dentistry
- CRISPR-Cas9
- Conservation
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