Kakimoto Y, Ito S, Abiru H, Kotani H, Ozeki M, Tamaki K, and Tsuruyama T:
Sorbin and SH3 Domain‐Containing Protein 2 is Released from Infarcted Heart in the Very Early Phase: Proteomic Analysis of Cardiac Tissues From Patients
J. Am. Heart Assoc. 2: e000565 (2013); originally published 16.12.2013; doi: 10.1161/JAHA.113.000565
In the course of this study the authors utilized laser microdissection to sample infarcted and healthy human cardiac tissue on a square millimeter scale. A very high sampling accuracy was needed because of subsequent proteomic analysis (LC-MS/MS) of the histologic samples, which is a highly sensitive method. Laser microdissection was used in this case to exclude unwanted tissue areas – like large capillaries or areas of fibrosis – that would distort mass spectrometric results.