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Super-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics

Plasma membrane dynamics are an important ruler of cellular activity, particularly through the interaction and diffusion dynamics of membrane-embedded proteins and lipids. FCS (fluorescence correlation spectroscopy) on an optical (confocal) microscope is a popular tool for investigating such dynamics. Unfortunately, its full applicability is con-strained by the limited spatial resolution of a conventional optical microscope. The present chapter depicts the combination of optical super-resolution STED (stimulated emission depletion) microscopy with FCS , and why it is an important tool for investigating molecular membrane dynamics in living cells. Compared with conventional FCS , the STED- FCS approach demonstrates an improved possibility to distinguish free from anomalous molecular diffusion, and thus to give new insights into lipid-protein interactions and the traditional lipid ‘raft’ theory.

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Eggeling C

Super-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics

Essays In Biochemistry Feb 15, 2015, 57 69-80; DOI: 10.1042/bse0570069