Leica Science Lab - Tag : CRISPR/Cas https://www.leica-microsystems.com//science-lab/tag/crispr/cas/ Article tagged with CRISPR/Cas en-US https://www.leica-microsystems.com/25143 Quantitative Imaging How FRET/FLIM Biosensors and Lifetime Imaging Helped Identify the Importance of Zinc for Human B-Cell Development In her latest studies, Dr. Mukta Deobagkar-Lele investigated the role of ZIP7, a zinc transporter protein, in modulating B cell activity and its effects on human immunity. During an interview with Dr. Luis Alvarez from Leica Microsystems, she describes the key findings of these studies published in her recent article in Nature Immunology. She also discusses her introduction to lifetime imaging with the SP8 FALCON and how this modality helps her explore B-cell biology and related immunodeficiencies. https://www.leica-microsystems.com/science-lab/how-fretflim-biosensors-and-lifetime-imaging-helped-identify-the-importance-of-zinc-for-human-b-cell-development/ Tue, 30 Jul 2019 09:10:00 +0000 PhD Mukta Deobagkar, Dr. Luis Alvarez https://www.leica-microsystems.com/24692 Quantitative Imaging Researchers use Functional Imaging to Elucidate the Role of Cellular Zn²⁺ in Human Immunity An international consortium of researchers has found a new mutation in a gene harboring a reticulum-to-cytoplasm zinc transporter. The gene is called ZIP7 and it is responsible for the early onset of infections in patients. These findings led them to uncover the role of ZIP7 in B cell development. To elucidate the cellular function of ZIP7, researchers used CRISPR-Cas9 mutagenesis to recreate the observed mutations in mice. They then studied primary cells with functional imaging and zinc biosensors to corroborate the mutations and see how cells handle zinc. https://www.leica-microsystems.com/science-lab/researchers-use-functional-imaging-to-elucidate-the-role-of-cellular-zn2-in-human-immunity/ Wed, 27 Feb 2019 23:00:00 +0000 Dr. Luis Alvarez https://www.leica-microsystems.com/19239 CRISPR-Cas9 Video: Genome Engineering with CRISPR-Cas9 Jennifer Doudna tells the story of how studying the way bacteria fight viral infection turned into a genomic engineering technology that has transformed molecular biology research. In 2013, Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact genomes. CRISPR-Cas9 is more precise, more efficient, and less expensive than other genome editing tools and, as a result, has facilitated a wide range of studies that were previously unachievable. https://www.leica-microsystems.com/science-lab/video-genome-engineering-with-crispr-cas9/ Fri, 09 Jun 2017 06:50:00 +0000 https://www.leica-microsystems.com/19530 CRISPR-Cas9 Video: Genome Editing with CRISPR-Cas9 This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, including the potential to treat human genetic disease. https://www.leica-microsystems.com/science-lab/video-genome-editing-with-crispr-cas9/ Thu, 08 Jun 2017 06:50:00 +0000 https://www.leica-microsystems.com/19232 Fluorescence Microscopy CRISPR-Cas9 Five Big Mysteries about CRISPR’s Origins Francisco Mojica was not the first to see CRISPR, but he was probably the first to be smitten by it. He remembers the day in 1992 when he got his first glimpse of the microbial immune system that would launch a biotechnology revolution. He was reviewing genome-sequence data from the salt-loving microbe Haloferax mediterranei and noticed 14 unusual DNA sequences, each 30 bases long. https://www.leica-microsystems.com/science-lab/five-big-mysteries-about-crisprs-origins/ Mon, 20 Feb 2017 08:49:00 +0000 https://www.leica-microsystems.com/19109 Confocal Microscopy Human NK Cell Development Requires CD56-mediated Motility and Formation of the Developmental Synapse While distinct stages of natural killer (NK) cell development have been defined, the molecular interactions that shape human NK cell maturation are poorly understood. Here we define intercellular interactions between developing NK cells and stromal cells which, through contact-dependent mechanisms, promote the generation of mature, functional human NK cells from CD34+ precursors. We show that developing NK cells undergo unique, developmental stage-specific sustained and transient interactions with developmentally supportive stromal cells, and that the relative motility of NK cells increases as they move through development in vitro and ex vivo. https://www.leica-microsystems.com/science-lab/human-nk-cell-development-requires-cd56-mediated-motility-and-formation-of-the-developmental-synapse/ Wed, 15 Feb 2017 07:36:00 +0000 Ph.D. Emily M. Mace https://www.leica-microsystems.com/18885 Fluorescence Microscopy CRISPR-Cas9 Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target sequence via Watson-Crick base pairing. It serves as a marker for the foreign nucleic acid. The second component is the Cas9 protein. It binds to the marked sequence and cuts it due to its nuclease activity. Because the base pairing RNA can be synthesized easily and then used to determine a target region, researchers have utilized this system in the laboratory for genome editing. https://www.leica-microsystems.com/science-lab/gene-editing-with-crisprcas9-breakthrough-in-genome-engineering/ Thu, 17 Nov 2016 18:54:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/18623 Fluorescence Microscopy CRISPR-Cas9 Video Talk: CRISPR-Cas – From a Bacterial Adaptive Immune System to a Genome Engineering Tool The CRISPR-Cas system was originally discovered as an adaptive immune system of bacteria and archaea to protect against viral attack. During this talk, given at Leica Microsystems in Wetzlar, Dr. Lennart Randau, MPI Marburg, presents this simple and fascinating system in detail. Furthermore, he introduces the adaption of the CRISPR-Cas system into a potent molecular biology tool, which is used heavily for genome editing. In addition to its influence on molecular biology, meanwhile the Cas9 nuclease also stimulates microscopy techniques e.g. to fluorescently label genomic loci in living cells. https://www.leica-microsystems.com/science-lab/video-talk-crispr-cas-from-a-bacterial-adaptive-immune-system-to-a-genome-engineering-tool/ Tue, 12 Jul 2016 04:13:00 +0000 Dr. Lennart Randau