Leica Science Lab - Tag : Collagen https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=1091&cHash=90e880ce9d6fed443e2bc9e7ca48d296 Article tagged with Collagen en-US https://www.leica-microsystems.com/18164 Super-Resolution TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion Collagen is the most abundant protein in the animal kingdom. It is of fundamental importance during development for cell differentiation and tissue morphogenesis as well as in pathological processes such as fibrosis and cancer cell migration. However, our understanding of the mechanisms of procollagen secretion remains limited. Here, we show that TFG organizes transitional ER (tER) and ER exit sites (ERESs) into larger structures. Depletion of TFG results in dispersion of tER elements that remain associated with individual ER-Golgi intermediate compartments (ERGICs) as largely functional ERESs. We show that TFG is not required for the transport and packaging of small soluble cargoes but is necessary for the export of procollagen from the ER. Our work therefore suggests a key relationship between the structure and function of ERESs and a central role for TFG in optimizing COPII assembly for procollagen export. https://www.leica-microsystems.com/science-lab/tfg-promotes-organization-of-transitional-er-and-efficient-collagen-secretion/ Tue, 14 Jun 2016 08:36:00 +0000 https://www.leica-microsystems.com/5929 Multiphoton Microscopy Confocal Microscopy Order versus Disorder In modern biomaterial design the generation of an environment mimicking some of the extracellular matrix features is envisaged to support molecular cross-talk between cells and scaffolds during tissue formation/remodeling. In bone substitutes chemical biomimesis has been particularly exploited; conversely, the relevance of pre-determined scaffold architecture for regenerated bone outputs is still unclear. https://www.leica-microsystems.com/science-lab/order-versus-disorder/ Thu, 19 Apr 2012 22:00:00 +0000 Paolo Giannoni, Paolo Bianco, Prof. Alberto Diaspro, Anna Tampieri, Ugo Valbusa, Giuseppe Firpo, Roberto Marotta, Monica Sandri, Paolo Bianchini, Rodolfo Quarto