Leica Science Lab - Tag : Tutorial https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=1261&cHash=2788b893bd6fc4d2a11fc3d92bad5368 Article tagged with Tutorial en-US https://www.leica-microsystems.com/28402 Surgical Microscopy Dentistry Neurosurgery Ophthalmology Otolaryngology (ENT) How to Drape an Overhead Surgical Microscope The tutorial features the Leica ARveo digital Augmented Reality microscope for complex neurosurgery. The procedure also applies to the Leica M530 OHX, OH6, OH5 and OH4. https://www.leica-microsystems.com/science-lab/how-to-drape-an-overhead-surgical-microscope/ Mon, 06 Apr 2020 13:14:00 +0000 https://www.leica-microsystems.com/28397 Surgical Microscopy Dentistry Neurosurgery Ophthalmology Otolaryngology (ENT) How to Drape a Surgical Microscope Before performing surgical procedures, it is important to drape the surgical microscope to ensure sterile working conditions. At Leica, we are committed to helping you with your surgical practice. In the below video, discover a step-by-step tutorial on draping your surgical microscope. The tutorial features the Leica PROvido multidisciplinary surgical microscope. The procedure also applies to the Leica M525 F50 microscope. https://www.leica-microsystems.com/science-lab/how-to-drape-a-surgical-microscope/ Mon, 06 Apr 2020 13:07:00 +0000 https://www.leica-microsystems.com/17667 Live-Cell Imaging Widefield Microscopy How to do a Proper Cell Culture Quick Check In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth must be monitored at regular intervals. This article describes a typical workflow for subculturing an adherent cell line with detailed illustrations of all of the necessary steps. https://www.leica-microsystems.com/science-lab/how-to-do-a-proper-cell-culture-quick-check/ Thu, 24 Mar 2016 09:18:00 +0000 PhD Tamara Straube, Claudia Müller https://www.leica-microsystems.com/9350 Fluorescence Microscopy Confocal Microscopy Live-Cell Imaging Handbook of Optical Filters for Fluorescence Microscopy Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and an optic that might be appropriate and optimal for one is both inappropriate and sub-optimal for another. https://www.leica-microsystems.com/science-lab/handbook-of-optical-filters-for-fluorescence-microscopy/ Tue, 06 Aug 2013 07:59:00 +0000 BSc. Jay Reichman https://www.leica-microsystems.com/6656 Live-Cell Imaging Quantitative Imaging Widefield Calcium Imaging with Calcium Indicator Fura2 In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are usually kept low, as Ca2+ often forms insoluble complexes with phosphorylated and carboxylated compounds. Typically cytosolic Ca2+ concentrations are in the range of 100 nM. In response to stimuli Ca2+ may either be released from external medium or internal stores to raise the Ca2+ concentration. https://www.leica-microsystems.com/science-lab/widefield-calcium-imaging-with-calcium-indicator-fura2/ Sun, 13 Jan 2013 23:00:00 +0000 Gabriele Burger, Simone Diehl https://www.leica-microsystems.com/6600 Live-Cell Imaging Confocal Microscopy Quantitative Imaging Label-free FLIM Many biological samples exhibit autofluorescence. Its often broad spectra can interfere with fluorescent labeling strategies. This application letter demonstrates how autofluorescence can serve as an intrinsic contrast in fluorescence lifetime imaging microscopy (FLIM) resulting in multi-color image stacks. https://www.leica-microsystems.com/science-lab/label-free-flim/ Thu, 09 Aug 2012 22:00:00 +0000 Dr. Constantin Kappel https://www.leica-microsystems.com/6602 Live-Cell Imaging Confocal Microscopy Quantitative Imaging FRET with FLIM FLIM combines lifetime measurements with imaging: lifetimes obtained for each image pixel are color-coded to produce additional image contrast. Thus, FLIM delivers information about the spatial distribution of a fluorescent molecule together with information about its biochemical status or nano-environment. A typical application of FLIM is FLIM-FRET. FRET is a well-established technique to study molecular interactions. It scrutinizes protein binding and estimates intermolecular distances on an Angström scale as well. https://www.leica-microsystems.com/science-lab/fret-with-flim/ Tue, 07 Aug 2012 22:00:00 +0000 Dr. Constantin Kappel https://www.leica-microsystems.com/6417 Image Processing for Widefield Microscopy Fluorescence microscopy is a modern and steadily evolving tool to bring light to current cell biological questions. With the help of fluorescent proteins or dyes it is possible to make discrete cellular components visible in a highly specific manner. A prerequisite for these kinds of investigations is a powerful fluorescence microscope. One special aim is the three-dimensional illustration of a structure to get an impression of full plasticity. This poses a certain problem for the experimenter using a classical light microscope. https://www.leica-microsystems.com/science-lab/getting-sharper-3d-images-of-thick-biological-specimens-with-widefield-microscopy/ Wed, 04 Jul 2012 22:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/5106 Live-Cell Imaging Introduction to Live-Cell Imaging The understanding of complex and fast cellular dynamics is an important step to get insight into biological processes. Therefore, today’s life science research more and more demands studying physiological events on the molecular level in real-time. https://www.leica-microsystems.com/science-lab/introduction-to-live-cell-imaging/ Tue, 03 Apr 2012 22:00:00 +0000 Dr. Thomas Veitinger https://www.leica-microsystems.com/5403 TIRF Microscopy Live-Cell Imaging Widefield Microscopy Total Internal Reflection Fluorescence (TIRF) Microscopy Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy delivers images with an outstandingly high axial resolution below 100 nm. This allows the observation of membrane-associated processes. https://www.leica-microsystems.com/science-lab/total-internal-reflection-fluorescence-tirf-microscopy/ Sun, 11 Mar 2012 23:00:00 +0000 Wymke Ockenga https://www.leica-microsystems.com/5405 Live-Cell Imaging TIRF Microscopy Widefield Microscopy Applications of TIRF Microscopy in Life Science Research The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or lasers, the evanescent field only penetrates the specimen by about 100 nm starting from the coverslip/medium interface. https://www.leica-microsystems.com/science-lab/applications-of-tirf-microscopy-in-life-science-research/ Sun, 11 Mar 2012 23:00:00 +0000 Wymke Ockenga, Dr. Thomas Veitinger https://www.leica-microsystems.com/5108 Live-Cell Imaging Confocal Microscopy Live-cell Imaging Techniques The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focusing on dynamic processes like cell migration, morphological changes of cells, organs or whole animals and physiological (e.g. changes of intracellular ion composition) events in living specimens in real time. https://www.leica-microsystems.com/science-lab/live-cell-imaging-techniques/ Thu, 23 Feb 2012 23:00:00 +0000 Dr. Thomas Veitinger, Dr. Zhongxiang Jiang https://www.leica-microsystems.com/4758 Live-Cell Imaging Neuroscience Ratiometric Imaging Many fundamental functions of a cell strongly depend on delicate, but nevertheless dynamic balances of ions (e.g. calcium, magnesium), voltage potentials and pH between the cell’s cytosol and the surrounding extracellular space. Ratiometric imaging allows reliable estimations of ion concentrations and pH or voltage changes by measuring fluorophore emission shifts. https://www.leica-microsystems.com/science-lab/ratiometric-imaging/ Sun, 11 Dec 2011 23:00:00 +0000 Dr. Thomas Veitinger https://www.leica-microsystems.com/4727 Neuroscience Live-Cell Imaging The Patch-Clamp Technique Especially in neuroscience, the physiology of ion channels has always been a major topic of interest. The development of the patch-clamp technique in the late 1970s has given electrophysiologists new prospects. It allows high-resolution current recordings not only of whole cells, but also of excised cellular patches. Even single-channel opening events can be investigated. However, with its complex technical, physical and biological background, the need for highly sensitive equipment and the huge amount of skills required of the experimenter, electrophysiology is still one of the most challenging methods in daily laboratory work. https://www.leica-microsystems.com/science-lab/the-patch-clamp-technique/ Wed, 09 Nov 2011 16:28:00 +0000 Dr. Sophie Veitinger