Leica Science Lab - Tag : Stellaris Confocal Re-Imagined https://www.leica-microsystems.com//science-lab/tag/stellaris-confocal-re-imagined/ Article tagged with Stellaris Confocal Re-Imagined en-US https://www.leica-microsystems.com/72758 Confocal Microscopy Kinetochore assembly during mitosis with TauSTED on 3D Three-dimensional organization of the mitotic spindle together with the distribution of CENP-C and BUB1 based on TauSTED with multiple STED lines (592, 660 and 775 nm) can provide insights on kinetochore assembly. https://www.leica-microsystems.com/science-lab/kinetochore-assembly-during-mitosis-with-tausted-on-3d/ Wed, 02 Jun 2021 10:52:00 +0000 Dr. Julia Roberti https://www.leica-microsystems.com/72767 Confocal Microscopy Fast 2 colour live cell dynamics with TauSTED gentle super-resolution TauSTED, with low photon budget and the fast detection scheme in our STELLARIS confocal platform, enables the tracking of highly dynamic processes in live cells. We can see in this example the dynamics of the cytoskeleton and membrane vesicles. https://www.leica-microsystems.com/science-lab/fast-2-colour-live-cell-dynamics-with-tausted-gentle-super-resolution/ Wed, 02 Jun 2021 10:52:00 +0000 Dr. Julia Roberti https://www.leica-microsystems.com/72738 Confocal Microscopy How to Quantify Changes in the Metabolic Status of Single Cells Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed. Now, STELLARIS 8 DIVE FALCON makes metabolic imaging accessible to every scientist, thanks to the integration of phasor analysis to easily use fluorescence lifetime information. https://www.leica-microsystems.com/science-lab/how-to-quantify-changes-in-the-metabolic-status-of-single-cells/ Thu, 27 May 2021 10:52:00 +0000 Corporate Communications https://www.leica-microsystems.com/71884 Super-Resolution Confocal Microscopy Benefits of TauContrast to Image Complex Samples In this interview, Dr. Timo Zimmermann talks about his experience with the application of TauSense tools and their potential for the investigation of demanding samples such as thick samples or oversized embryos. As the head of the Advanced Light Microscopy Unit at the Centre for Genomic Regulation (CRG) in Barcelona, Dr. Timo Zimmermann tested the STELLARIS 5 confocal system there in 2020. https://www.leica-microsystems.com/science-lab/interview-with-timo-zimmermann-february-8th-2021/ Mon, 12 Apr 2021 10:50:00 +0000 Corporate Communications, Dr. Timo Zimmermann https://www.leica-microsystems.com/44724 Confocal Microscopy Fluorescence Microscopy A New World of Confocal Applications with the Next Generation White Light Lasers As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is translated in having to use multiple fluorescent labels. Unfortunately, the visualization of multiple events with confocal microscopy is limited by the number of fluorescent probes that can be simultaneously imaged without incurring bleed-through artifacts due to significant spectral overlap. Using a confocal microscope with an integrated White Light Laser (WLL) enables tuning excitation and detection to perfectly match the spectral profile of the dyes in your sample, giving you the flexibility to perform multicolor experiments while obtaining clear, sharp images. https://www.leica-microsystems.com/science-lab/a-new-world-of-confocal-applications-with-the-next-generation-white-light-lasers/ Thu, 03 Dec 2020 14:20:00 +0000 https://www.leica-microsystems.com/44422 Confocal Microscopy Live-Cell Imaging Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these interactions with higher spatiotemporal resolution than ever before. Here we present two examples from the field of HIV research that make use of an innovative quantitative imaging approach as well as cutting edge fluorescence lifetime-based confocal microscopy methods to gain novel insights into how HIV fuses to cell membranes and enters the cell. https://www.leica-microsystems.com/advanced-quantitative-fluorescence-microscopy-to-probe-the-molecular-dynamics-of-viral-entry/ Wed, 11 Nov 2020 09:48:00 +0000 PhD Ben Libberton https://www.leica-microsystems.com/30582 Super-Resolution Time-resolved STED microscopy Introduced more than 30 years ago, stimulated emission depletion (STED) microscopy has raised to a standard and widely used method for imaging in the life sciences. Thanks to continuous technological progress, STED microscopy can now provide effective sub-diffraction spatial resolution, while preserving most of the useful aspects of fluorescence microscopy, such as optical sectioning, molecular specificity and sensitivity, and live-cell compatibility. https://www.leica-microsystems.com/science-lab/time-resolved-sted-microscopy/ Thu, 03 Sep 2020 12:04:00 +0000 Ph.D. Giuseppe Vicidomini https://www.leica-microsystems.com/30720 Confocal Microscopy Obtain maximum information from your specimen with LIGHTNING LIGHTNING is an adaptive process for extraction of information that reveals fine structures and details, otherwise simply not visible, fully automatically. Unlike traditional technologies, that use a global set of parameters for the full image, LIGHTNING calculates an appropriate set of parameters for each voxel to uncover details with the highest fidelity. https://www.leica-microsystems.com/science-lab/obtain-maximum-information-from-your-specimen-with-lightning/ Sun, 30 Aug 2020 12:49:00 +0000 https://www.leica-microsystems.com/30707 Confocal Microscopy Expanding the frontiers of confocal live cell imaging To address the specific challenges of live cell confocal imaging and help researchers get closer to the truth, Leica Microsystems has re-imagined confocal microscopy, designing its entirely new STELLARIS platform from the ground up. Here we explore how STELLARIS unlocks the full power and potential of live cell studies by overcoming many common limitations and fully integrating fluorescence lifetime-based information to add a new dimension to your research. https://www.leica-microsystems.com/science-lab/expanding-the-frontiers-of-confocal-live-cell-imaging/ Thu, 20 Aug 2020 10:39:00 +0000 https://www.leica-microsystems.com/30238 Confocal Microscopy Super-Resolution Nanoscopy meets Lifetime: Introducing τ-STED τ-STED combines the optical signals from STED and the photophysical information from the fluorescence lifetime at unprecedented speeds. This approach to STED uses phasor analysis in a novel way. τ-STED enables an increased STED resolution and elimination of uncorrelated background noise, even at low excitation and STED powers. https://www.leica-microsystems.com/science-lab/nanoscopy-meets-lifetime-introducing-t-sted/ Mon, 06 Jul 2020 10:48:00 +0000 https://www.leica-microsystems.com/29648 Confocal Microscopy Explore Innovative Techniques to Separate Fluorophores with Overlapping Spectra In this article we explore several strategies you can take to improve the separation of fluorophores and increase the number of fluorescent probes you can distinguish in your sample. https://www.leica-microsystems.com/science-lab/explore-innovative-techniques-to-separate-fluorophores-with-overlapping-spectra/ Tue, 05 May 2020 09:59:00 +0000 PhD Liz Roquemore https://www.leica-microsystems.com/28598 Confocal Microscopy STELLARIS White light lasers When it comes to choosing fluorescent probes for your multi-color experiments, you shouldn’t have to compromise. Now you can advance beyond conventional excitation sources that limit your fluorophore choices and multiplexing capabilities. The STELLARIS next-generation White Light Laser (WLL), paired with optimally matched detectors from our Power HyD family of detectors, gives you complete spectral freedom to choose and combine exactly the right probes to answer your experimental questions. https://www.leica-microsystems.com/stellaris-white-light-lasers/ Tue, 28 Apr 2020 15:25:00 +0000 https://www.leica-microsystems.com/29614 Confocal Microscopy TauSense Technolgy Imaging Tools Leica Microsystems’ TauSense technology is a set of imaging modes based on fluorescence lifetime. Found at the core of the STELLARIS confocal platform, it will revolutionize your imaging experiments. Whatever your sample or staining procedure, fluorescence lifetime information is always there. Now TauSense gives you access to this additional information and expands the potential of your research with the possibilities provided by different TauSense modes. https://www.leica-microsystems.com/science-lab/add-an-extra-dimension-to-confocal-results-with-tausense/ Tue, 28 Apr 2020 15:13:00 +0000 https://www.leica-microsystems.com/28595 Confocal Microscopy The Power HyD detector family In STELLARIS, we have pushed the limits of our detector technology, so you can push the boundaries of your research. Our newly designed Power HyD family consists of three different type of detectors designed to fit your confocal applications. https://www.leica-microsystems.com/the-power-hyd-detector-family/ Tue, 28 Apr 2020 07:28:00 +0000 PhD Ben Libberton https://www.leica-microsystems.com/28460 Confocal Microscopy How to Uncover Hidden Dimensions in Research with Lifetime Imaging Learn how fluorescence lifetime imaging adds information depth to your confocal experiments and reveals novel insights that are difficult or impossible to discover using conventional intensity-based analysis. https://www.leica-microsystems.com/science-lab/how-to-uncover-hidden-dimensions-in-research-with-lifetime-imaging/ Wed, 15 Apr 2020 11:27:00 +0000 PhD Liz Roquemore https://www.leica-microsystems.com/28307 Confocal Microscopy Learn how to Remove Autofluorescence from your Confocal Images Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as different ways to remove it, from simple media fixes to powerful technological solutions. Removing this background is essential to correctly interpreting small differences in the signals present within your sample. Find out how in this article. https://www.leica-microsystems.com/science-lab/learn-how-to-remove-autofluorescence-from-your-confocal-images/ Mon, 23 Mar 2020 09:58:00 +0000 PhD Ben Libberton