Leica Science Lab - Tag : Organoids + 3D Cell Culture https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=1266&cHash=2f7c4f9387f9070e0b47f55544f54f02 Article tagged with Organoids + 3D Cell Culture en-US https://www.leica-microsystems.com/24935 Fluorescence Microscopy Live-Cell Imaging Widefield Microscopy Stereo Microscopy Image Gallery: THUNDER Imager To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve this using Computational Clearing our new opto-digital technology. The result is high-speed, high-quality imaging of a large diversity of three-dimensional samples, including model organisms, tissue sections, and 3D cell cultures. Take a look at these images to see how THUNDER Imagers are already helping researchers to reveal the finest structural details even deep within a sample. Once you have seen the haze-free image quality achieved with THUNDER Imagers, find out more on our product page. Learn how THUNDER Imagers combine Computational Clearing with the speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes so you can decode 3D biology in real-time. https://www.leica-microsystems.com/science-lab/galleries/image-gallery-thunder-imager/ Wed, 20 Jan 2021 06:56:00 +0000 https://www.leica-microsystems.com/19049 Multiphoton Microscopy Live-Cell Imaging Confocal Microscopy Fluorescence Microscopy Neuroscience Multiphoton Microscopy Publication List Multiphoton Microscopy is an advanced technique for imaging thick samples. Applications range from the visualization of the complex architecture of the whole brain to the study of tumor development and metastasis or the responses of the immune system in living animals. On this regularly updated reference list you can find selected publications on reseach using multiphoton microscopy. https://www.leica-microsystems.com/science-lab/multiphoton-microscopy-publication-list/ Thu, 20 Feb 2020 08:47:00 +0000 https://www.leica-microsystems.com/26677 Quantitative Imaging SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM SP8 FALCON (FAst Lifetime CONtrast) is a fast and completely integrated fluorescence lifetime imaging microscopy (FLIM) confocal platform. SP8 FALCON delivers video-rate FLIM with pixel-by-pixel quantification, thanks to a novel concept for measuring fluorescence lifetimes built on fast electronics and sensitive spectral hybrid detectors. Photon arrival times are recorded at count rates typical for standard confocal imaging. The system has ultra-short dead time and powerful built-in algorithms for data acquisition and analysis. The deep integration of FLIM into the confocal platform provides easy access to complex FLIM experiments. https://www.leica-microsystems.com/science-lab/a-novel-concept-in-fluorescence-lifetime-imaging-enabling-video-rate-confocal-flim/ Thu, 17 Oct 2019 09:37:00 +0000 Bernd Widzgowski, Bram van den Broek, Dr. Giulia Ossato, PhD Kees Jalink, Dr. Lioba Kuschel, Dr. Julia Roberti, Frank Hecht, Dr. Luis Alvarez https://www.leica-microsystems.com/27569 Confocal Microscopy Improve 3D Cell Biology Workflow with Digital Light Sheet Microscopy Understanding the sub-cellular mechanisms in carcinogenesis is of crucial importance for cancer treatment. Popular cellular models comprise cancer cells grown as monolayers. But this approach disregards the three-dimensional (3D) interaction of tumor cells with their surrounding microenvironment. To understand the development and progression of malignancy in a close to nature context, the characterization of cancer microenvironments is crucial. https://www.leica-microsystems.com/science-lab/improve-3d-cell-biology-workflow-with-digital-light-sheet-microscopy/ Sun, 10 Mar 2019 08:53:00 +0000 https://www.leica-microsystems.com/20297 Confocal Microscopy Image Gallery: FLIM for high-speed investigation of molecular interactions SP8 FALCON (FAst Lifetime CONtrast) is the fast, and fully integrated platform for fluorescence lifetime imaging (FLIM) and analysis. It enables the observation of fast dynamic cellular events with lifetime contrasts between fluorescent molecules down to sub-second time scales. https://www.leica-microsystems.com/science-lab/galleries/image-gallery-flim-for-high-speed-investigation-of-molecular-interactions/ Wed, 04 Jul 2018 22:00:00 +0000 Dr. Johanna Berndt https://www.leica-microsystems.com/20208 Quantitative Imaging FLIM FRET and Biosensors: Versatile Tools for Biomedical Research Fluorescence Lifetime Imaging (FLIM) in combination with Förster Resonance Energy Transfer (FRET) has proven to be very beneficial for investigations in biomedical research for a wide range of structural elements and dynamic changes in cells. FRET allows to monitor molecular interactions, as the FRET signal depends strongly on the distance of the two FRET partners. This allows to investigate interaction of molecules, like ligand-receptor pairs, protein-protein interactions or interactions of effectors with DNA. https://www.leica-microsystems.com/science-lab/flim-fret-and-biosensors-versatile-tools-for-biomedical-research/ Sun, 10 Jun 2018 22:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/19686 Live-Cell Imaging Introduction to Mammalian Cell Culture Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research would be unthinkable. This article gives an overview of mammalian cell culture systems. Mainly, they can be categorized according to their morphology, as well as cell type and organization. Moreover, you can find basic information about the correct growth conditions and what kind of microscope you need to watch your cells. https://www.leica-microsystems.com/science-lab/introduction-to-mammalian-cell-culture/ Thu, 10 Aug 2017 10:54:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/17437 Fluorescence Microscopy Stereo Microscopy Investigating the Influence of Hormone Signaling Pathways on Mammary Gland Development and the Onset of Breast Cancer Over their entire lifetime, 1 out of 8 women can suffer from breast cancer. The risk of developing breast cancer (mammary carcinogenesis) increases with a woman’s age and is related to her reproductive history. The chance of mammary carcinogenesis is less for women who give birth to a child before the age of 30. However, it is now known that the risk of breast cancer also can depend on the number of times a woman experiences elevated blood serum progesterone levels, in relation to the menstrual cycle, before her first pregnancy. https://www.leica-microsystems.com/science-lab/investigating-the-influence-of-hormone-signaling-pathways-on-mammary-gland-development-and-the-onset-of-breast-cancer/ Thu, 21 Jan 2016 11:12:00 +0000 MD Cathrin Brisken, PhD Renuga Devi Rajaram, PhD Duje Buric, MSc Ayyakkannu Ayyanan, PhD James DeRose