Leica Science Lab - Tag : LMD https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=131&cHash=c3fa9f6ab8dc5ec3a299fdfeeb3d1b5e Article tagged with LMD en-US https://www.leica-microsystems.com/3815 Laser Microdissection Neuroscience Forensics Laser Microdissection Publication List This list presents current publications in the major application fields for laser microdissection and is updated monthly. https://www.leica-microsystems.com/science-lab/laser-microdissection-publication-list/ Fri, 15 Jan 2021 09:55:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/26359 Laser Microdissection Medicinal Use of Qianhu Plant Roots This study focused on tissue-specific metabolite profiling and comparison of various parts of bolting and unbolting Peucedanum praeruptorum Dunn (Qianhu in Chinese). The qualitative and quantitative results were used to empirically clarify the suitability of Qianhu for medicinal purposes. https://www.leica-microsystems.com/science-lab/tissue-specific-metabolite-profiling-on-the-different-parts-of-bolting-and-unbolting-peucedanum-praeruptorum-dunn-qianhu/ Wed, 06 Nov 2019 09:54:00 +0000 Eugenia Petoukhov https://www.leica-microsystems.com/27638 Laser Microdissection How to improve your RNA Analysis Workflow with Laser Microdissection Brain research: Single neuron excision for RNA analysis - Improve your workflow with laser microdissection - Parkinson’s disease is a common progressive neurodegenerative disorder. It is connected with cell death of dopamine-releasing neurons in the substantia nigra. Differences in gene expression patterns between individual dopamine-releasing neurons of disease affected and healthy individuals allow defining target genes for therapies. For RNA analysis, single cell resolution is crucial, as investigating the whole tissue is meaningless. Analyzing mixtures of dopamine-releasing neurons and all other brain cells distorts the result. https://www.leica-microsystems.com/science-lab/how-to-improve-your-rna-analysis-workflow-with-laser-microdissection/ Sun, 17 Mar 2019 10:30:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/27643 Laser Microdissection How to improve your Alzheimer Protein Analysis with Laser Microdissection Brain Research: Collect pure starting material for proteomics - Improve your workflow with Laser Microdissection - Many brain diseases result from protein malfunction, misfolding and agglutination. For this reason protein analysis is the key to understanding causes of, and discovering therapies for, many cerebral defects. https://www.leica-microsystems.com/science-lab/how-to-improve-your-alzheimer-protein-analysis-with-laser-microdissection/ Sat, 02 Mar 2019 10:53:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/27591 Laser Microdissection How to improve your DNA Mutation Analysis Workflow with Laser Microdissection DNA mutations lead to abnormal proteins or missing functional proteins, which can cause cells to multiply uncontrollably and become cancerous. To find and understand the underlying mutation for a specific cancer type, extraction of pure tumor material is extremely important. This is challenging especially if only a limited amount of cancerous tissue is available (small metastasis, for example). Laser Microdissection allows for the extraction of purely cancerous tissue without contaminating your sample with healthy cells. DNA sequencing will lead to valid and reproducible results helping to identify the cancer causing DNA mutation easily as no masking by contaminating healthy cells can occur. https://www.leica-microsystems.com/science-lab/how-to-improve-your-dna-mutation-analysis-workflow-with-laser-microdissection/ Fri, 08 Feb 2019 12:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/20653 Laser Microdissection Live Cell Isolation by Laser Microdissection Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections, laser microdissection may be applied for live cell isolation. Unlike other well-established and widely used techniques for live cell isolation and single cell cloning—such as FACS, MACS, cloning by limited dilution, and so on—laser microdissection allows for capturing live cells and cell colonies without their detachment from the carrier. https://www.leica-microsystems.com/science-lab/live-cell-isolation-by-laser-microdissection/ Tue, 16 Oct 2018 22:00:00 +0000 https://www.leica-microsystems.com/19390 Laser Microdissection Correlation of Phenotype and Genotype – microscopic samples and their biomolecular backgrounds Seeing is believing, this is what makes microscopes, imaging systems, or box-type imagers so important for researchers in many different fields like pathology research, cancer research, neuroscience, and developmental research. Correlation of phenotype (what you can see) and genotype (genetic background) is highly desired but often biased by whole tissue approaches as no real solution is available to reliably separate the single cells of interest and surrounding tissue. Thus, results of DNA mutation analysis (sequencing), gene expression profiling (quantitative real-time PCR, qPCR, microarray, digital PCR), next generation sequencing (NGS) or mass spectrometry (MS) approaches can give mixed results as no pure starting material for the biomolecular methods of choice is available. https://www.leica-microsystems.com/science-lab/correlation-of-phenotype-and-genotype-microscopic-samples-and-their-biomolecular-backgrounds/ Thu, 20 Apr 2017 07:08:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/17941 Laser Microdissection Workflows & Protocols: Plant Laser Microdissection During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection using the Leica LMD systems was demonstrated. One special focus was on plant dissection which needs a high laser power. https://www.leica-microsystems.com/science-lab/workflows-protocols-plant-laser-microdissection/ Mon, 06 Jun 2016 05:22:00 +0000 Dr. Falk Schlaudraff, Dr. Christoph Greb https://www.leica-microsystems.com/17939 Laser Microdissection Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a perfect tool for this purpose and how this was taught in the course of a workshop held in Brazil. With the Leica LMD system pure tumor material can be selected and dissected for downstream analysis to ensure 100% pure starting material without any risk of cross contamination with healthy cells. https://www.leica-microsystems.com/science-lab/workflows-protocols-laser-microdissection-for-pathology-and-cancer-research/ Wed, 04 May 2016 11:30:00 +0000 Dr. Falk Schlaudraff, Dr. Christoph Greb https://www.leica-microsystems.com/16814 Laser Microdissection Neuroscience Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as it allows to separate distinct brain layers or even to isolate individual neurons. https://www.leica-microsystems.com/science-lab/workflows-protocols-connecting-microscopy-and-molecular-biology-in-neuroscience/ Fri, 04 Dec 2015 16:01:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/16808 Laser Microdissection Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome. Another topic was the dissection of individual chromosomes from chromosome spreads. Leica specialists held a short training session with the LMD. After this, new LMD users were able to run the system and practiced how to dissect chromosomes and collect single chromosomes for downstream analysis. https://www.leica-microsystems.com/science-lab/workflows-protocols-how-to-isolate-individual-chromosomes-with-laser-microdissection/ Thu, 03 Dec 2015 20:06:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/16110 Laser Microdissection Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or sequencing techniques. Here we describe the successful combination of the Leica Microsystems LMD system and Qiagen kits for purification of nucleic acids even from little amounts. https://www.leica-microsystems.com/science-lab/workflows-protocols-how-to-use-a-leica-laser-microdissection-system-and-qiagen-kits-for-successful-rna-analysis/ Fri, 10 Jul 2015 14:26:00 +0000 Dr. Falk Schlaudraff https://www.leica-microsystems.com/5080 Laser Microdissection Consumables for Laser Microdissection There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own individual configuration whatever their field of research. https://www.leica-microsystems.com/science-lab/application-specific-consumables-for-laser-microdissection/ Mon, 04 May 2015 11:47:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/14824 Laser Microdissection Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen Tissue As most tissue specimens contain a mixture of different cell types including epithelial cells, stromal cells and immune cells, selection of the cells of interest is of utmost importance for the accurate determination of gene/microRNA expression. Laser capture microdissection enables the researcher to obtain homogeneous ultrapure cell selections from heterogeneous starting material. https://www.leica-microsystems.com/science-lab/total-rna-isolation-after-laser-capture-microdissection-of-human-cervical-squamous-epithelial-cells-from-fresh-frozen-tissue/ Fri, 20 Mar 2015 17:24:00 +0000 https://www.leica-microsystems.com/14774 Laser Microdissection Neuroscience Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and infiltrate brain parenchyma, they closely interact with a changing micro-environmental landscape that sustains tumor cell invasion. https://www.leica-microsystems.com/science-lab/identification-of-molecular-pathways-facilitating-glioma-cell-invasion-in-situ/ Mon, 22 Dec 2014 09:35:00 +0000 https://www.leica-microsystems.com/13495 Laser Microdissection Preparation of Laser Microdissected Plant Cell and Tissue Material for Microchemical Analysis Secondary plant metabolites are involved in various plant physiological and biochemical processes, allowing plants to interact successfully with their biotic and abiotic environment. Distribution patterns of these compounds at the tissue and cellular level show how plant defense systems are organized to manage environmental challenges in a targeted and precise way. https://www.leica-microsystems.com/science-lab/preparation-of-laser-microdissected-plant-cell-and-tissue-material-for-microchemical-analysis/ Tue, 24 Jun 2014 09:08:00 +0000 PhD Dirk Hölscher, PhD Sara Agnolet, PhD Jingjing Fang, PhD Jonathan Gershenzon, PhD Oliver Kayser, PhD Sheng-Hong Li, PhD Bernd Schneider https://www.leica-microsystems.com/2469 Laser Microdissection Neuroscience The Morbus Parkinson Puzzle A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this disease have been identified. In the case of the genetic familial form, for example, it has been possible to identify various genes that have a causal influence for M. Parkinson. https://www.leica-microsystems.com/science-lab/the-morbus-parkinson-puzzle/ Thu, 27 Mar 2014 11:11:00 +0000 Dr. Falk Schlaudraff, Dr. Olaf Spörkel https://www.leica-microsystems.com/12787 Laser Microdissection Sorbin and SH3 Domain‐Containing Protein 2 Is Released from Infarcted Heart in the Very Early Phase: Proteomic Analysis of Cardiac Tissues From Patients Many animal experiments have been performed to date to elucidate the mechanism of cardiac ischemic injury, and these studies have enhanced our understanding of cardiac remodeling following hypoxic stress. Clinical studies using samples from admitted patients have also been conducted to discover diagnostic or prognostic biomarkers for acute myocardial infarction (AMI). https://www.leica-microsystems.com/science-lab/sorbin-and-sh3-domain-containing-protein-2-is-released-from-infarcted-heart-in-the-very-early-phase-proteomic-analysis-of-cardiac-tissues-from-patients/ Wed, 05 Mar 2014 19:11:00 +0000 https://www.leica-microsystems.com/12449 Laser Microdissection Neuroscience Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol. Sufficient RNA (~40–60 ng) is recovered from 3,000–4,000 motor neurons to allow downstream RNA analysis by RNA-seq and qRT-PCR. https://www.leica-microsystems.com/science-lab/production-of-rna-for-transcriptomic-analysis-from-mouse-spinal-cord-motor-neuron-cell-bodies-by-laser-capture-microdissection/ Thu, 30 Jan 2014 09:11:00 +0000 https://www.leica-microsystems.com/11133 Laser Microdissection Laser Microdissection (LMD) and Fancy Applications New and far-reaching applications have recently been opened up in the field of laser microdissection. In addition to conventional dissection, the laser microdissection system (LMD) of Leica Microsystems is an excellent tool for marking relevant structures, offering highly specific laser manipulation of selected areas. This laser marking function is useful for applications such as CLEM, NanoSIMS as well as in the live cell sector. https://www.leica-microsystems.com/science-lab/laser-microdissection-lmd-and-fancy-applications/ Mon, 09 Dec 2013 13:02:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/10668 Laser Microdissection Video: How to Achieve Fast Precise Cutting Lines in Laser Microdissection This video explains the laser beam deflection principle of Leica Microsystems‘ laser microdissection systems (Leica LMD). The laser, the beam expander, motorized attenuator, motorized iris diaphragm for limiting the aperture for cutting and the scan unit are situated in the instrument’s special incident light axis. The scan unit consists of two prism wedges whose thickness and wedge angle are chosen so that the laser beam, after passing through the two prism wedges, is directed precisely through the center of the back focal plane of the objective. https://www.leica-microsystems.com/science-lab/video-how-to-achieve-fast-precise-cutting-lines-in-laser-microdissection/ Mon, 18 Nov 2013 14:32:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/7275 Laser Microdissection Stem Cells in the Aging Process – the Signal Protein p53 as "Guardian of the Genome" The physiological process of aging is an elementary part of life. The processes responsible for the aging process and the effect they have is one of the key focuses of biomedical research. Dr. Yvonne Begus-Nahrmann researches molecular mechanisms in stem cells and their role in aging and tumor development at the Göttingen University Medical Center. Signal proteins such as p53 and p21 appear to play an important role in this context. To investigate these proteins in specific cell types, the cells are isolated from organ tissue with the aid of laser microdissection. https://www.leica-microsystems.com/science-lab/stem-cells-in-the-aging-process-the-signal-protein-p53-as-guardian-of-the-genome/ Thu, 08 Nov 2012 23:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille, Dr. Yvonne Begus-Nahrmann https://www.leica-microsystems.com/6773 Laser Microdissection K-ATP Channels in Dopamine Substantia Nigra Neurons Control Bursting and Novelty-induced Exploration Phasic activation of the dopamine (DA) midbrain system in response to unexpected reward or novelty is critical for adaptive behavioral strategies. This activation of DA midbrain neurons occurs via a synaptically triggered switch from low-frequency background spiking to transient high-frequency burst firing. We found that, in medial DA neurons of the substantia nigra (SN), activity of ATP-sensitive potassium (K-ATP) channels enabled NMDA-mediated bursting in vitro as well as spontaneous in vivo burst firing in anesthetized mice. https://www.leica-microsystems.com/science-lab/k-atp-channels-in-dopamine-substantia-nigra-neurons-control-bursting-and-novelty-induced-exploration/ Thu, 04 Oct 2012 22:00:00 +0000 Julia Schiemann, Prof. Birgit Liss, Dr. Gaby Schneider, Ph.D. Kareem A. Zaghloul, Dr. Peter J. Magill, Prof. Susumu Seino, Markus Bingmer, Dr. Verena Klose, Dr. Falk Schlaudraff, Prof. Jochen Röper https://www.leica-microsystems.com/5268 Laser Microdissection Neuroscience Region-Specific Gene Expression in Adult Mouse CNS Tissues Different areas of the Central Nervous System (CNS) display a specific and selective gene expression profile. Here, we used the Laser Microdissection system Leica LMD6500 to study region-specific mRNA expression in the adult mouse retina and hippocampus. https://www.leica-microsystems.com/science-lab/region-specific-gene-expression-in-adult-mouse-cns-tissues/ Tue, 21 Feb 2012 23:00:00 +0000 Andrea Messina, Mark Dunleavy, Ph.D. Paola Sgadò, Valentina Adami, Ph.D. Yuri Bozzi, Ph.D. Simona Casarosa https://www.leica-microsystems.com/4849 Laser Microdissection Neuroscience TED Talk of Allan Jones: Mapping the Human Brain How can we begin to understand the complexity of the human brain and the way it works? The same way we begin to understand a city: by making a map. In this talk, Allan Jones shows how his team is mapping which genes are turned on in each tiny region, and how it all connects up. https://www.leica-microsystems.com/science-lab/ted-talk-of-allan-jones-mapping-the-human-brain/ Fri, 02 Dec 2011 16:42:00 +0000 Ph.D. Allan Jones https://www.leica-microsystems.com/4392 Laser Microdissection Cell Cultures and Laser Microdissection Many of the discoveries that are now being made in cell division and differentiation, the relationships between single cells and cell organelles, treatments of cells with pharmaceutic substances, etc. would not be possible without live cell cultures. Allowing morphological and biochemical observations of single cells under different experimental conditions, they provide a unique source of information. https://www.leica-microsystems.com/science-lab/cell-cultures-and-laser-microdissection/ Fri, 07 Oct 2011 09:15:57 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/3819 Laser Microdissection An Introduction to Laser Microdissection Laser microdissection is a highly selective process for preparing samples for DNA, RNA and protein analysis. It is a microscope-controlled manipulation technique for the precise separation of samples and tissue using a focused laser beam. https://www.leica-microsystems.com/science-lab/an-introduction-to-laser-microdissection/ Tue, 28 Jun 2011 15:39:57 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/3835 Laser Microdissection Applications of Laser Microdissection Laser microdissection and laser micromanipulation are suitable for gaining a differentiated insight into the function of genes and proteins, and are used for a wide range of applications in neurobiology and immunology as well as in the developmental and cell biology of animal and plant organisms. https://www.leica-microsystems.com/science-lab/applications-of-laser-microdissection/ Sun, 26 Jun 2011 22:00:00 +0000 M.Sc., Cornelia Gilbrich-Wille https://www.leica-microsystems.com/3862 Laser Microdissection Neuroscience Users Report on the Relevance of Laser Microdissection for Their Research Results Laser dissection is used in a large number of research fields, e.g. neurology, cancer research, plant analysis. Here, user report on the research results they have attained by using laser microdissection. https://www.leica-microsystems.com/science-lab/users-report-on-the-relevance-of-laser-microdissection-for-their-research-results/ Tue, 12 Apr 2011 22:00:00 +0000 https://www.leica-microsystems.com/3833 Laser Microdissection Neuroscience The Mitochondrial Hypothesis of Ageing Why do we grow old? Research scientists have been looking for an answer to this question for many years – particularly against the background of the increase in neurodegenerative diseases among older people such as Morbus Parkinson. https://www.leica-microsystems.com/science-lab/the-mitochondrial-hypothesis-of-ageing/ Mon, 07 Sep 2009 16:11:00 +0000 Dr. Andreas Bender, Dipl. oec.-troph. Anja Schué