Leica Science Lab - Tag : Depth of Field https://www.leica-microsystems.com//science-lab/tag/depth-of-field/ Article tagged with Depth of Field en-US https://www.leica-microsystems.com/5098 Stereo Microscopy Basics in Microscopy Quality Assurance Key Factors to Consider When Selecting a Stereo Microscope Stereo microscopes are often nicknamed the “workhorse” of the lab or production site. Users spend many hours looking through the eyepieces inspecting, observing, documenting, or dissecting samples. Careful assessment of the relevant applications for which the stereo microscope is needed is key to long-lasting, satisfactory use. https://www.leica-microsystems.com/science-lab/factors-to-consider-when-selecting-a-stereo-microscope/ Thu, 24 Sep 2020 07:00:00 +0000 B.Sc. Elisabeth Ippel, PhD James DeRose, Daniel Goeggel https://www.leica-microsystems.com/24965 Digital Microscopy Stereo Microscopy Quality Assurance How to create EDOF (Extended Depth of Focus) images Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the optional Extended Depth of Focus (EDOF) function of the LAS X software from Leica Microsystems. Acquisition of EDOF images of a printed circuit board, recorded from low to high magnification using a Leica microscope, are shown as an example. The operation of EDOF by acquiring a z-stack of images in 3 modes is demonstrated. https://www.leica-microsystems.com/science-lab/how-to-create-edof-extended-depth-of-focus-images/ Wed, 08 May 2019 22:00:00 +0000 Clinton Smith, Mike Horz https://www.leica-microsystems.com/19544 Stereo Microscopy Digital Microscopy Coming to Grips with Biological Information Through Flexible Organic Electronics: Developing Bendable and Stretchable Biosensors and Device What do you associate with the word sensor? Perhaps technologies delivering automation in factories and other production sites? That may be what comes to mind, but advances in organic electronics are now driving the rapid development of biological sensors that measure physiological signals when in contact with the skin, organs, and other parts of the body. https://www.leica-microsystems.com/science-lab/coming-to-grips-with-biological-information-through-flexible-organic-electronics-developing-bendable-and-stretchable-biosensors-and-device/ Wed, 28 Jun 2017 10:14:00 +0000 Kuniko Yagura, PhD James DeRose https://www.leica-microsystems.com/18742 Basics in Microscopy Widefield Microscopy Image Gallery: Application Options with Leica DM2500 LED Microscope The number of applications in microscopy can be as manifold as life itself. Different specimens demand different contrast methods to get a decent result. Unstained cells normally do not show a very high contrast. Researchers approach this problem by either staining the specimen with dyes respectively fluorescent molecules; or adding contrast by manipulating the light passing through. https://www.leica-microsystems.com/science-lab/galleries/image-gallery-application-options-with-leica-dm2500-led-microscope/ Tue, 23 Aug 2016 11:45:00 +0000 Dr. Christoph Greb, PhD Tamara Straube https://www.leica-microsystems.com/15606 Confocal Microscopy Light Sheet Microscopy Confocal and Digital Light Sheet Imaging Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the limited depth of field. Our eye-lens (an optical imaging instrument) has the same trouble, but our brain smartly removes all not-in-focus information before the signal reaches conscious cognition. https://www.leica-microsystems.com/science-lab/confocal-and-digital-light-sheet-imaging/ Mon, 11 May 2015 07:15:00 +0000 Dr. Petra Haas https://www.leica-microsystems.com/14612 Surgical Microscopy Neurosurgery Ophthalmology FusionOptics in Neurosurgery and Ophthalmology – for a Larger 3D Area in Focus Neurosurgeons and ophthalmologists deal with delicate structures, deep or narow cavities and tiny structures with vitally important functions. A clear, three-dimensional view on the surgical field is thus indispensable for the outcome of the operation and the patient’s safety. Until now, an increased depth of field for a larger three-dimensional area in focus was only achievable by reducing the resolution. A new technology is able to overcome this challenge. https://www.leica-microsystems.com/science-lab/fusionoptics-in-neurosurgery-and-ophthalmology-for-a-larger-3d-area-in-focus/ Wed, 08 Oct 2014 19:17:00 +0000 https://www.leica-microsystems.com/6596 Digital Microscopy Quality Assurance 3D Visualization of Surface Structures One of the main features of a digital microscope is the speed and ease with which it enables surface models to be created of macroscopic and microscopic structures. In a qualitative evaluation, these provide a better understanding and a more detailed documentation of the specimen. In addition, quantification of the surface provides valuable information about the composition of the surface and its wear. Which specimens are suitable for use with a Leica digital microscope, and what are the limitations of the method used? https://www.leica-microsystems.com/science-lab/3d-visualization-of-surface-structures/ Wed, 19 Sep 2012 22:00:00 +0000 Daniel Goeggel, Georg Schlaffer https://www.leica-microsystems.com/2557 Basics in Microscopy Stereo Microscopy Widefield Microscopy How Sharp Images Are Formed In microscopy, depth of field is often seen as an empirical parameter. In practice it is determined by the correlation between numerical aperture, resolution and magnification. For the best possible visual impression, the adjustment facilities of modern microscopes produce an optimum balance between depth of field and resolution – two parameters which in theory are inversely correlated. https://www.leica-microsystems.com/science-lab/how-sharp-images-are-formed/ Wed, 28 Apr 2010 22:00:00 +0000 Ruedi Rottermann, Dipl. Geol. Peter Bauer https://www.leica-microsystems.com/5350 Stereo Microscopy Quality Assurance FusionOptics – Combines high resolution and depth of field for ideal 3D optical Images A study carried out jointly by Leica Microsystems and the Institute of Neuroinformatics at the University of Zurich and Swiss Federal Institute of Technology provided the basis for an innovation in stereomicroscopy: FusionOptics™. The significant performance increase attained by FusionOptics™ is highly valuable for everyday work at the microscope. https://www.leica-microsystems.com/science-lab/fusionoptics-combines-high-resolution-and-depth-of-field-for-ideal-3d-optical-images/ Thu, 17 Apr 2008 22:00:00 +0000 Daniel Goeggel, Dipl. oec.-troph. Anja Schué, Dr. Daniel Kiper