Leica Science Lab - Tag : Resonant Scanner https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=175&cHash=27a2f7fb36af5482c6519e310db4fdc6 Article tagged with Resonant Scanner en-US https://www.leica-microsystems.com/24698 Confocal Microscopy What is a Resonant Scanner? A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition speed is required to track fast processes, especially in living samples. It has the additional benefit of providing a better fluorescence signal and reduced photobleaching. https://www.leica-microsystems.com/science-lab/what-is-a-resonant-scanner/ Sun, 10 Mar 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/24694 Confocal Microscopy What is a Tandem Scanner? A Tandem Scanner is an assembly of two different types of scanning together in one system for true confocal point scanning. The Tandem Scanner consists of a three-mirror scanning base with the x-scanner exchangeable with a motorized device. This combination allows scanning of large areas with high scan-resolution by a FOV-scanner and of very fast processes by a resonant scanner, both within the same instrument. https://www.leica-microsystems.com/science-lab/what-is-a-tandem-scanner/ Thu, 28 Feb 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/13262 Quantitative Imaging FRAP with TCS SP8 Resonant Scanner Fast FRAP experiments need a sufficient number of measurement points for meaningful interpretation and fitting analysis. To study very fast translocational processes, the use of a resonant scanner (RS) is preferred. The advantage in using FRAP with the RS is that statistics are much better in experiments that require fast acquisition: If the half time of recovery is about 0.5 sec you may have only about 3 to 4 data points using the conventional scanner, whereas with the resonant scanner you can get about 20 data points. https://www.leica-microsystems.com/science-lab/frap-with-tcs-sp8-resonant-scanner/ Tue, 26 Jan 2016 15:39:00 +0000 Dr. Jan Schröder https://www.leica-microsystems.com/15203 Confocal Microscopy Live-Cell Imaging Video: High Speed Scanning – With two Scanners in one System High speed scanning is necessary to image rapidly changing biological processes. With traditional scanning techniques, imaging speed is limited by the number of fluorophores in a specimen. And, rapid acquisition often comes at the cost of image resolution. https://www.leica-microsystems.com/science-lab/video-high-speed-scanning-with-two-scanners-in-one-system/ Tue, 24 Feb 2015 15:15:00 +0000 PhD Christopher Vega https://www.leica-microsystems.com/14063 Confocal Microscopy Multiphoton Microscopy Smart Control for Resonant Galvo Scanners High time-resolution confocal microscopy (HTRCLSM) requires fast scanning devices. Whereas non-resonant galvo scanners allow full position control, but only at slow speed, resonant scanners allow ~25,000 lines per second, but offer much less positioning freedom. To still allow zoom and pan functions, several approaches have been tried, with varying success. The Leica confocal microscopes od the TCS series use a very smart solution that enables stepless zooming with short switching times. https://www.leica-microsystems.com/science-lab/smart-control-for-resonant-galvo-scanners/ Fri, 01 Aug 2014 08:29:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/4126 Confocal Microscopy Fluorescence Microscopy Brighter Fluorescence by Resonant Scanning Fast True Confocal Scanning reduces photobleaching and increases the fluorescence yield at identical acquisition times. The long-lasting triplet state (or any other “dark state”) is less populated when the illumination is applied in shorter pulses at the same intensity. Consequently, more fluorochromes are available for the fluorescence process (brighter images) and fewer fluorochromes disintegrate from triplet states or excited triplet states (less bleaching). https://www.leica-microsystems.com/science-lab/brighter-fluorescence-by-resonant-scanning/ Wed, 03 Aug 2011 08:42:54 +0000 Dr. Rolf T. Borlinghaus