Leica Science Lab - Tag : GFP https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=205&cHash=14e81184ea835cc7e9f969cc46e3cf01 Article tagged with GFP en-US https://www.leica-microsystems.com/19773 Fluorescence Microscopy Basics in Microscopy The Fundamentals and History of Fluorescence and Quantum Dots At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and trace anything from whole organisms to single proteins and beyond. In this article, we will examine what is meant by "fluorescence", the history and basic physics behind its definition, the discovery and application of Green Fluorescent Protein (GFP) and a look at the rapidly expanding field of fluorescent probes including Quantum Dots. https://www.leica-microsystems.com/science-lab/the-fundamentals-and-history-of-fluorescence-and-quantum-dots/ Wed, 15 Nov 2017 16:44:00 +0000 PhD Martin Wilson https://www.leica-microsystems.com/18108 Fluorescence Microscopy Confocal Microscopy The Oncogenic Triangle of HMGA2, LIN28B and IGF2BP1 Antagonizes Tumor-Suppressive Actions of the let-7 Family The tumor-suppressive let-7 microRNA family targets various oncogene-encoding mRNAs. We identify the let-7 targets HMGA2, LIN28B and IGF2BP1 to form a let-7 antagonizing self-promoting oncogenic triangle. Surprisingly, 3′-end processing of IGF2BP1 mRNAs is unaltered in aggressive cancers and tumor-derived cells although IGF2BP1 synthesis was proposed to escape let-7 attack by APA-dependent (alternative polyadenylation) 3′ UTR shortening. https://www.leica-microsystems.com/science-lab/the-oncogenic-triangle-of-hmga2-lin28b-and-igf2bp1-antagonizes-tumor-suppressive-actions-of-the-let-7-family/ Fri, 17 Jun 2016 13:49:00 +0000 https://www.leica-microsystems.com/13935 Fluorescence Microscopy Video Talk by Roger Tsien: Fluorescent Proteins Live cell imaging has been revolutionized by the discovery of the green fluorescent protein (GFP). This lecture covers the history of GFP, how it folds and becomes fluorescent, how it has been mutated to produce additional colors (blue, cyan, yellow), and the discovery of red fluorescent proteins from corals. It also covers novel photoswitchable and photoactivatible fluorescent proteins, whose color can be changed by light, and new infrared fluorescent proteins. https://www.leica-microsystems.com/science-lab/video-talk-by-roger-tsien-fluorescent-proteins/ Wed, 07 Oct 2015 16:55:00 +0000 PhD Roger Y. Tsien https://www.leica-microsystems.com/16035 Fluorescence Microscopy Live-Cell Imaging Basics in Microscopy Fluorescent Proteins Illuminate Cell Biology Green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria and GFP-like fluorescent proteins from other animals have had an important role in the technical innovations that have driven these advances. This poster provides a comprehensive user's guide to fluorescent proteins and sensors , their key properties and the cell biological questions to which they can be applied. https://www.leica-microsystems.com/science-lab/fluorescent-proteins-illuminate-cell-biology/ Fri, 18 Sep 2015 09:03:00 +0000 https://www.leica-microsystems.com/16124 Basics in Microscopy A Brief History of Light Microscopy – From the Medieval Reading Stone to Super-Resolution The history of microscopy begins in the Middle Ages. As far back as the 11th century, plano-convex lenses made of polished beryl were used in the Arab world as reading stones to magnify manuscripts. However, the further development of these lenses into the first microscopes cannot be attributed to any one person. It took the ideas and designs of many scientists and scholars to produce instruments capable of strong magnification. https://www.leica-microsystems.com/science-lab/a-brief-history-of-light-microscopy-from-the-medieval-reading-stone-to-super-resolution/ Tue, 08 Sep 2015 17:41:00 +0000 Wymke Ockenga https://www.leica-microsystems.com/14080 Fluorescence Microscopy Live-Cell Imaging Spectral and Structural Comparison Between Bright and Dim Green Fluorescent Proteins in Amphioxus The cephalochordate Amphioxus naturally co-expresses fluorescent proteins (FPs) with different brightness, which thus offers the rare opportunity to identify FP molecular feature/s that are associated with greater/lower intensity of fluorescence. Here, we describe the spectral and structural characteristics of green FP (bfloGFPa1) with perfect (100%) quantum efficiency yielding to unprecedentedly-high brightness, and compare them to those of co-expressed bfloGFPc1 showing extremely-dim brightness due to low (0.1%) quantum efficiency. https://www.leica-microsystems.com/science-lab/spectral-and-structural-comparison-between-bright-and-dim-green-fluorescent-proteins-in-amphioxus/ Thu, 28 Aug 2014 15:54:00 +0000 https://www.leica-microsystems.com/12428 Confocal Microscopy Fluorescence Microscopy Colonization of Potato Rhizosphere by GFP-Tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 Shown on Large Sections of Roots Using Enrichment Sample Preparation and Confocal Laser Scanning Microscopy The ability to colonize the host plants’ rhizospheres is a crucial feature to studyin the case of Plant Growth Promoting Rhizobacteria (PGPRs) with potential agricultural applications. In this work, we have created GFP-tagged derivatives of three candidate PGPRs: Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44. https://www.leica-microsystems.com/science-lab/colonization-of-potato-rhizosphere-by-gfp-tagged-bacillus-subtilis-mb732-pseudomonas-sp-p482-and-ochrobactrum-sp-a44-shown-on-large-sections-of-roots-using-enrichment-sample-preparation-and-confocal-laser-scanning-microscopy/ Fri, 02 May 2014 15:04:00 +0000 https://www.leica-microsystems.com/5831 Fluorescence Microscopy Widefield Microscopy Fluorescent Proteins – Introduction and Photo Spectral Characteristics The prospects of fluorescence microscopy changed dramatically with the discovery of fluorescent proteins in the 1950s. The starting point was the detection of the jellyfish Aequorea victoria green fluorescent protein (GFP) by Osamo Shimomura. Hundreds of GFP mutants later, the range of fluorescent proteins reaches from the blue to the red spectrum. https://www.leica-microsystems.com/science-lab/fluorescent-proteins-introduction-and-photo-spectral-characteristics/ Mon, 09 Apr 2012 22:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/5256 Fluorescence Microscopy Fluorescent Proteins - From the Beginnings to the Nobel Prize Fluorescent proteins are the fundament of recent fluorescence microscopy and its modern applications. Their discovery and consequent development was one of the most exciting innovations for life sciences in the last century and the starting point of the deciphering of numberless natural phenomena. https://www.leica-microsystems.com/science-lab/fluorescent-proteins-from-the-beginnings-to-the-nobel-prize/ Thu, 16 Feb 2012 23:00:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/4713 Fluorescence Microscopy Modern Fluorescent Proteins and their Biological Applications Here we present two review articles on fluorescent proteins and their biological applications. These first article reviews our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. The second article focuses on novel monomeric RFPs and their application for studying gene expression, nuclear localization, and dynamics using advanced imaging. https://www.leica-microsystems.com/science-lab/modern-fluorescent-proteins-and-their-biological-applications/ Fri, 18 Nov 2011 12:50:00 +0000 https://www.leica-microsystems.com/10218 Super-Resolution Live-Cell Imaging Nanoscopy in a Living Multicellular Organism Expressing GFP We report superresolution fluorescence microscopy in an intact living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP)-fusion proteins. We also superresolve, by stimulated emission depletion (STED) microscopy, living cultured cells, demonstrating that STED microscopy with GFP can be widely applied. https://www.leica-microsystems.com/science-lab/nanoscopy-in-a-living-multicellular-organism-expressing-gfp/ Wed, 15 Jun 2011 12:38:00 +0000 https://www.leica-microsystems.com/4303 Confocal Microscopy Obese and Slim Yeast Cells Lipids are on everyone’s lips nowadays, whether ω-3/6 fatty acids, good and bad cholesterol or just plain fat that has the annoying habit of accumulating on our hips. Serious diseases such as obesity, arteriosclerosis and type 2 diabetes mellitus are directly connected with lipid metabolism disorders. https://www.leica-microsystems.com/science-lab/obese-and-slim-yeast-cells/ Thu, 21 Aug 2008 22:00:00 +0000 Univ.-Prof., Dipl. Ing., Dr. techn. Sepp Dieter Kohlwein https://www.leica-microsystems.com/7970 CLEM EM Sample Preparation CLEM: Combining the Strengths of Light and Electron Microscopy In recent years light microscopy studies have been dominated by live cell imaging while electron microscopy has been used for high-resolution studies. Latterly, there has been increasing interest in combining these techniques. This combination is called Correlative Light Electron Microscopy (CLEM). Due to the high resolution made possible by electron microscopy, artefacts induced during preparation of a sample can, however, also be clearly seen. https://www.leica-microsystems.com/science-lab/clem-combining-the-strengths-of-light-and-electron-microscopy/ Mon, 09 Apr 2007 22:00:00 +0000 Professor Paul Verkade https://www.leica-microsystems.com/4394 Confocal Microscopy Quantitative Imaging Multiphoton Microscopy 4D Photoactivation of pa-GFP in Living Cells Using Two-Photon Excitation Laser Scanning Microscopy We report about two-photon activation of a photoactivatable derivative of the Aequorea Victoria green fluorescent protein (pa-GFP). This special form of the molecule increases its fluorescence intensity when excited by 488 nm after irradiation with high intensity light at 413 nm. Two-photon photoactivation produces an effective real three-dimensional (3D) localization of the molecular switching of pa-GFP in the bright state. https://www.leica-microsystems.com/science-lab/4d-photoactivation-of-pa-gfp-in-living-cells-using-two-photon-excitation-laser-scanning-microscopy/ Sat, 14 Oct 2006 22:00:00 +0000 Prof. Alberto Diaspro, Massimiliano Garrè, Pietro Transidico, Dario Parazzoli, Sara Barozzi, Ph.D. Giuseppe Vicidomini, Davide Mazza, Valentina Caorsi, Ph.D. Illaria Testa, Mario Faretta