Leica Science Lab - Tag : Label free https://www.leica-microsystems.com//science-lab/tag/label-free/ Article tagged with Label free en-US https://www.leica-microsystems.com/27706 Confocal Microscopy Multiphoton Microscopy How to Quantify Label-free Blood Flow The application note “Label-free Blood Flow Quantification” is a guide for data acquisition and analysis of blood flow using label-free third harmonic generation (THG) imaging. https://www.leica-microsystems.com/science-lab/how-to-quantify-label-free-blood-flow/ Fri, 11 Sep 2020 12:28:00 +0000 https://www.leica-microsystems.com/28596 Coherent Raman Scattering (CRS) Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative spectroscopic imaging of label-free or chemically tagged specimens: From cell and tissue biology to medical research, materials science, and microfabrication; Probing 3D cellular model systems (organoids) and tissues for preclinical & translational research in cancer, neurodegenerative diseases, immunology, and digestive disorders; Multi-modal confocal nonlinear optical microscopy, combining CARS, SRS, Second-Harmonic Generation, and 2-Photon Fluorescence for advanced tissue-based diagnostics; Monitoring tissue penetration of pharmacological and cosmetic compounds https://www.leica-microsystems.com/science-lab/vibrational-spectroscopic-imaging-in-cells-tissues-and-model-organisms/ Wed, 29 Apr 2020 08:46:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/27674 Confocal Microscopy Coherent Raman Scattering (CRS) How to prepare samples for Stimulated Raman Scattering Stimulated Raman Scattering (SRS) is an emerging microscopy technique that images the vibrations of chemical bonds within a specimen. It allows researchers to probe the endogenous chemistry or biochemistry of their specimen without the need for exogenous labels. https://www.leica-microsystems.com/science-lab/how-to-prepare-samples-for-stimulated-raman-scattering/ Tue, 11 Feb 2020 08:59:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/4592 Coherent Raman Scattering (CRS) Step by Step Guide to the Molecular Basics of CARS Microscopy CARS (Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. The crucial advantage of this method is that the sample remains almost unaffected. This tutorial explains the molecular basics on Coherent Anti-Stokes Raman Scattering. https://www.leica-microsystems.com/science-lab/step-by-step-guide-to-the-molecular-basics-of-cars-microscopy/ Mon, 16 Dec 2019 11:38:00 +0000 Dr. Stefanie Degenhartt https://www.leica-microsystems.com/27272 Coherent Raman Scattering (CRS) Stimulated Raman Scattering Microscopy Probes Neurodegenerative Disease Despite decades of research, the molecular mechanisms underlying some of the most severe neurodegenerative diseases, such as Alzheimer’s or Parkinson’s, remain poorly understood. The progression of these diseases often correlates with the appearance of abnormal aggregations of proteins and lipids in affected brain tissues. There is hope that detailed investigations of these pathological structures might yield new insights into the disease mechanisms. Hence, there is a strong demand for advanced analytical techniques that can probe these structures in intact tissues. Here, we show that stimulated Raman scattering (SRS) microscopy can provide label-free, high-resolution, chemically specific characterization of tissue pathologies associated with neurodegenerative diseases. https://www.leica-microsystems.com/science-lab/stimulated-raman-scattering-microscopy-probes-neurodegenerative-disease/ Wed, 20 Nov 2019 11:32:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/4421 Coherent Raman Scattering (CRS) Coherent Raman Scattering Microscopy Publication List CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their molecules. The two most important CRS techniques are Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS). The biochemical image contrast of CRS is in many ways complementary to the molecular contrast obtained in fluorescence microscopy. A second crucial advantage of these methods is that they preserve the specimen/sample in a near pristine state. This reference list presents current and basic papers on CRS microscopy. https://www.leica-microsystems.com/science-lab/cars-publication-list/ Tue, 08 Oct 2019 22:00:00 +0000 Dr. Volker Schweikhard https://www.leica-microsystems.com/26267 Fluorescence Microscopy Stereo Microscopy Improved tree-ring visualization using autofluorescence Autofluorescence can be a very practical tool for the visualization of growth rings in wood, especially when these structures are hard to distinguish, like in tropical trees. Tree rings are largely used for the investigation of growth rate, forest dynamics and management, paleoclimate and climate change, among other applications. https://www.leica-microsystems.com/science-lab/improved-tree-ring-visualization-using-autofluorescence/ Tue, 23 Jul 2019 16:00:00 +0000 Ph.D. Piero Bagnaresi, PhD James DeRose https://www.leica-microsystems.com/24562 Confocal Microscopy Kaggle Competition for Multi-label Classification of Cell Organelles in Proteome Scale Human Protein Atlas Data The Cell Atlas, a part of the Human Protein Atlas, was created by the group of Prof. Emma Lundberg at the SciLifeLab, KTH Royal Institute of Technology, in Stockholm, Sweden. Currently, she is a visiting professor at Stanford University through the support of the Chan Zuckerberg Initiative. The Cell Atlas was created, in large part, using data acquired with Leica confocal instruments. In the scope of the Kaggle competition regarding the Human Protein Atlas Image Classification Prof. Lundberg gave an interview to Dr. Constantin Kappel from Leica Microsystems. https://www.leica-microsystems.com/science-lab/kaggle-competition-for-multi-label-classification-of-cell-organelles-in-proteome-scale-human-protein-atlas-data/ Sun, 09 Dec 2018 23:00:00 +0000 Dr. Constantin Kappel https://www.leica-microsystems.com/24510 Confocal Microscopy You Shall Not Pass! Time-gated Detection Takes Care of Endogenous Fluorescence in Plant Research. Yutaka Kodama is Associate Professor at the Biosciences Education Research Center, Utsunomiya University, Japan. He graduated from Saga University, Faculty of Agriculture, in 2004 and received his PhD from the Graduate School of Biological Science at the Nara Institute of Science and Technology in 2007. https://www.leica-microsystems.com/science-lab/you-shall-not-pass-time-gated-detection-takes-care-of-endogenous-fluorescence-in-plant-research/ Tue, 13 Nov 2018 23:00:00 +0000 https://www.leica-microsystems.com/15645 Confocal Microscopy Live-Cell Imaging Label-free in vivo Imaging of Myelinated Axons in Health and Disease with Spectral Confocal Reflectance Microscopy We report a new technique for high-resolution in vivo imaging of myelinated axons in the brain, spinal cord and peripheral nerve that requires no fluorescent labeling. This method, based on spectral confocal reflectance microscopy (SCoRe), uses a conventional laser scanning confocal system to generate images by merging the simultaneously reflected signals from multiple lasers of different wavelengths. https://www.leica-microsystems.com/science-lab/label-free-in-vivo-imaging-of-myelinated-axons-in-health-and-disease-with-spectral-confocal-reflectance-microscopy/ Fri, 01 Jul 2016 09:29:00 +0000 https://www.leica-microsystems.com/15430 Confocal Microscopy Multiphoton Microscopy Live-Cell Imaging Third Harmonic Generation Microscopy – Label-Free 3D-Tissue Imaging and Blood Flow Characterization THG microscopy as special variants of multiphoton microscopy. Third Harmonic Generation (THG) microscopy is a non-fluorescent multi-photon technique that combines the advantages of label-free imaging with restriction of signal generation to the focal spot of the scanning laser. It allows three-dimensional imaging of refraction index mismatches and of hemoglobin. https://www.leica-microsystems.com/science-lab/third-harmonic-generation-microscopy-label-free-3d-tissue-imaging-and-blood-flow-characterization/ Sat, 11 Apr 2015 17:34:00 +0000 Dr. Steffen Dietzel https://www.leica-microsystems.com/13917 Confocal Microscopy Live-Cell Imaging Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis Diagnosing bacterial infection (BI) remains a challenge for the attending physician. An ex vivo infection model based on human fixed polymorphonuclear neutrophils (PMNs) gives an autofluorescence signal that differs significantly between stimulated and unstimulated cells. We took advantage of this property for use in an in vivo pneumonia mouse model and in patients hospitalized with bacterial pneumonia. https://www.leica-microsystems.com/science-lab/analysis-of-autofluorescence-in-polymorphonuclear-neutrophils-a-new-tool-for-early-infection-diagnosis/ Wed, 29 Oct 2014 12:21:00 +0000 https://www.leica-microsystems.com/9374 Coherent Raman Scattering (CRS) Unmask the Hidden without Probes: CARS Fluorescence microscopy assumed a pivotal role in cell biology once it was possible to stain cell components selectively by fluorescing dyes. One of the first explorers of targeted stainings, Paul Ehrlich, had the idea that something that stains specifically should also kill specifically – which was associated with the term “magic bullet”, the essential idea of chemotherapy. His group discovered Salvarsan, a tailored drug against syphilis – though not specific enough not to cause substantial side effects. Screening many fluorescent dyes led to a long list of stainings which are used in histology, including dyes like DAPI or hematoxylin and eosin. https://www.leica-microsystems.com/science-lab/unmask-the-hidden-without-probes-cars/ Thu, 21 Mar 2013 23:00:00 +0000 Dr. Rolf T. Borlinghaus, Dr. Stefanie Degenhartt https://www.leica-microsystems.com/5901 Coherent Raman Scattering (CRS) CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules Coherent anti-Stokes Raman scattering (CARS) microscopy is a technique that generates images based on the vibrational signatures of molecules. This imaging methods does not require labeling, yet molecular specific information can be obtained from a range of important bio-molecular compounds. https://www.leica-microsystems.com/science-lab/cars-microscopy-imaging-characteristic-vibrational-contrast-of-molecules/ Mon, 16 Jul 2012 22:00:00 +0000 Ph.D. Eric Olaf Potma https://www.leica-microsystems.com/4662 Coherent Raman Scattering (CRS) An Introduction to CARS Microscopy CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which are based on vibrational contrast and chemical selectivity. The crucial advantage of this method is that the sample remains almost unaffected. With CARS, new samples that could not be stained due to unavailability of the appropriate dye are now accessible. https://www.leica-microsystems.com/science-lab/an-introduction-to-cars-microscopy/ Fri, 04 Nov 2011 14:36:46 +0000 Dr. Stefanie Degenhartt https://www.leica-microsystems.com/3669 Coherent Raman Scattering (CRS) Multiphoton Microscopy Good Vibrations In recent years, new molecular imaging techniques, such as coherent anti-Stokes Raman scattering microscopy (CARS), have been developed for rapid vibrational imaging of living cells. https://www.leica-microsystems.com/science-lab/good-vibrations/ Tue, 12 Apr 2011 22:00:00 +0000 Ph.D. Eric Olaf Potma, Dr. Bernd Sägmüller https://www.leica-microsystems.com/2743 Confocal Microscopy Coherent Raman Scattering (CRS) Multiphoton Microscopy CARS and Confocal The most important drawback of single-photon and multiphoton confocal microscopy is the need to label the specimen. CARS (Coherent Anti-Stokes Raman Spectroscopy) addresses this issue because it is non-toxic, non-destructive, and minimally invasive. https://www.leica-microsystems.com/science-lab/cars-and-confocal/ Mon, 01 Nov 2010 23:00:00 +0000 Dr. Stefanie Degenhartt, Ph.D. Vanessa Lurquin