Leica Science Lab - Tag : Light Microscopy https://www.leica-microsystems.com//science-lab/tag/light-microscopy/ Article tagged with Light Microscopy en-US https://www.leica-microsystems.com/19036 Basics in Microscopy Live-Cell Imaging Fluorescence Microscopy Widefield Microscopy Factors to Consider When Selecting a Research Microscope An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the configuration and features of the microscope are crucial for its application coverage, ranging from brightfield through fluorescence microscopy to live-cell imaging. This article provides a brief overview of the relevant microscope features and wraps up the key questions one should consider when selecting a research microscope. https://www.leica-microsystems.com/science-lab/factors-to-consider-when-selecting-a-research-microscope/ Thu, 24 Sep 2020 10:10:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/30934 Metallography Quality Assurance Challenges Faced When Manually Rating Non-Metallic Inclusions (NMIs) to Determine Steel Quality Rapid, accurate, and reliable rating of non-metallic inclusions (NMIs) is instrumental for the determination of steel quality. This article describes the challenges that arise from manual NMI rating, which is commonly used in steel production and component manufacturing, when striving for an efficient and cost-effective steel quality evaluation. https://www.leica-microsystems.com/science-lab/challenges-faced-when-manually-rating-non-metallic-inclusions-nmis-to-determine-steel-quality/ Wed, 23 Sep 2020 15:17:00 +0000 PhD James DeRose, PhD David R. Barbero https://www.leica-microsystems.com/30553 Metallography Quality Assurance Criteria to Consider When Choosing a Digital Solution for Steel Quality Rating For the efficient and fast rating of non-metallic inclusions to determine steel quality, deciding which digital rating solution to use can be a challenge. This article discusses the important criteria that users should consider when choosing a digital steel quality rating solution. Does the solution allow inclusion rating based on multiple international and regional steel quality standards which can be updated with future changes? Are user-defined standards possible? https://www.leica-microsystems.com/science-lab/criteria-to-consider-when-choosing-a-digital-solution-for-steel-quality-rating/ Wed, 02 Sep 2020 10:12:00 +0000 PhD James DeRose, Thomas Locherer https://www.leica-microsystems.com/29779 Metallography Quality Assurance Top Issues Related to Standards for Rating Non-Metallic Inclusions in Steel Supplying components and products made of steel to users worldwide can require that a single batch be compliant with multiple steel quality standards. This user demand creates significant challenges for suppliers. The standards specify rigorous methods for rating non-metallic inclusions in steel, as the inclusions have a strong influence on quality. Rating the steel quality helps ensure that products and components meet performance and safety specifications. https://www.leica-microsystems.com/science-lab/top-issues-related-to-standards-for-rating-non-metallic-inclusions-in-steel/ Fri, 19 Jun 2020 09:58:00 +0000 PhD James DeRose, PhD David R. Barbero, Thomas Locherer https://www.leica-microsystems.com/29928 Quality Assurance Metallography Analyzing non-metallic inclusions in steel Oftentimes we find ourselves caught up in tedious analyses by reticle and comparison chart, time-consuming double-evaluation according to several standards or subjective inspection results with a bias from different users. In this webinar, Dr. Nicol Ecke will talk about the advantages of automated analysis of non-metallic inclusions using LAS X Steel Expert*. Learn how this will help you get the reliable, unbiased and standard-compliant results you are aiming for faster and easier than ever before. https://www.leica-microsystems.com/science-lab/analyzing-non-metallic-inclusions-in-steel/ Tue, 02 Jun 2020 11:17:00 +0000 https://www.leica-microsystems.com/29922 Metallography Quality Assurance Performing Elemental Analysis down to the Micro Scale If you work in electronic component analysis, you will be familiar with the many challenges posed. Whether you are identifying metallic particles or checking product authenticity, it’s important to get the right information quickly and easily. In this webinar, Dr. Konstantin Kartaschew will explain how to obtain spatially resolved information on the chemical composition of microstructures using the Chemical Micro Analyzer LIBS-System. https://www.leica-microsystems.com/science-lab/performing-elemental-analysis-down-to-the-micro-scale/ Tue, 02 Jun 2020 10:28:00 +0000 https://www.leica-microsystems.com/19946 Metallography Quality Assurance Rate the Quality of Your Steel: Free Webinar and Report This webinar and report describe optimal microscopy solutions for rating steel quality in terms of non-metallic inclusions and reviews the various international and regional standards concerning rigorous quality assessment methods, e.g., EN 10247, ASTM E45, DIN 50602, and ISO 4967. https://www.leica-microsystems.com/science-lab/rate-the-quality-of-your-steel-free-webinar-and-report/ Tue, 28 Apr 2020 12:19:00 +0000 Dionis Diez, PhD James DeRose, Thomas Locherer https://www.leica-microsystems.com/24702 EM Sample Preparation Integrated Cryo-tomography Workflow Analyzing purified protein structures in a cellular context is key to understanding how proteins and protein complexes function. Thermo Fisher Scientific and Leica Microsystems developed the integrated cryo-tomography workflow to enable high resolution structural analysis, done in a controlled and effective way, of proteins in their native environments. In this webinar, the speakers will explain the unique features of the hardware and software integration and guide you step-by-step through the workflow. https://www.leica-microsystems.com/science-lab/changing-the-way-we-look-at-the-inner-workings-of-cells/ Sun, 03 Mar 2019 23:00:00 +0000 https://www.leica-microsystems.com/19763 Metallography Quality Assurance Forensics See the Structure with Microscopy - Know the Composition with Laser Spectroscopy The advantages of a 2-in-1 materials analysis solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) for simultaneous visual and chemical inspection are described in this report. The basic principles of the 2-in-1 solution and a comparison between it and other common materials analysis methods, such scanning electron microscopy (SEM), are explained to demonstrate how a rapid, efficient workflow is achieved. A 2-in-1 analysis solution can reduce significantly the cost and time for obtaining material image and composition data. Such data are instrumental in assuring quality and reliability to make confident decisions quickly during production, quality control, failure analysis, and research and development in industries and fields, such as automotive and metallurgy. https://www.leica-microsystems.com/science-lab/see-the-structure-with-microscopy-know-the-composition-with-laser-spectroscopy/ Mon, 11 Jun 2018 13:39:00 +0000 PhD James DeRose, Dr. Kay Scheffler https://www.leica-microsystems.com/20117 Metallography Leica is MAD about LIBS When analyzing material for cleanliness testing, users normally just want a simple way to know whether the particle under scrutiny is normal debris or something more risky. Users can now “LIBS” it to acquire rapidly the composition and then move onto the next manufacturing step. https://www.leica-microsystems.com/science-lab/leica-is-mad-about-libs/ Sun, 22 Apr 2018 22:00:00 +0000 Mike Tjepkema, PhD James DeRose https://www.leica-microsystems.com/19234 Basics in Microscopy Optimization of the Interplay of Optical Components for Aberration free Microscopy Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to achieve appropriate resolution. However, besides intentional effects, all optical components have also unwanted intrinsic influence on light, resulting in aberrations. This article highlights optical elements and their physical parameters involved in this process. Based on this, it gives a historical overview of philosophies about how to cope with aberration reduction. Seeing the microscope as a whole system turned out to be beneficial, leading to the harmonization of its constituents for optimal microscopic results. https://www.leica-microsystems.com/science-lab/optimization-of-the-interplay-of-optical-components-for-aberration-free-microscopy/ Wed, 31 May 2017 08:58:00 +0000 Karl-Heinz Schade, Peter Euteneuer, Dipl. Phys. Arnold Müller-Rentz https://www.leica-microsystems.com/15098 Basics in Microscopy Video Talk by Kurt Thorn: The Abbe Diffraction Experiment This lecture describes the famous experiments of Ernst Abbe which showed how diffraction of light by a specimen (and interference with the illuminating light) gives rise to an image and how collection of diffracted light defines the resolution of the microscope. These concepts are demonstrated by using a diffraction grating as a specimen and visualizing and comparing the diffraction pattern in the back focal plane as well as the image in the image plane. https://www.leica-microsystems.com/science-lab/video-talk-by-kurt-thorn-the-abbe-diffraction-experiment/ Mon, 13 Feb 2017 12:12:00 +0000 PhD Kurt Thorn https://www.leica-microsystems.com/17636 Widefield Microscopy Basics in Microscopy Infinity Optical Systems “Infinity Optics” refers to the concept of a beam path with parallel rays between the objective and the tube lens of a microscope. Flat optical components can be brought into this “Infinity Space” without influencing image formation, which is critical for the utilization of contrast methods such as DIC or fluorescence. Modern microscopy techniques require the addition of multiple optical instruments, such as light sources or laser devices, into the infinite light path. Different approaches to fulfill this need have emerged and are described here. https://www.leica-microsystems.com/science-lab/infinity-optical-systems/ Fri, 11 Mar 2016 16:28:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/13927 Basics in Microscopy Video Talk by Jeff Lichtman: Point Spread Function An infinitesimally small point appears in the microscope as a spot with a certain size, blurred in the z-direction and with concentric rings around it. This "point spread function" reveals many of the optical properties of your microscope. This lecture explains why and how the microscope images a point as a point spread function. https://www.leica-microsystems.com/science-lab/video-talk-by-jeff-lichtman-point-spread-function/ Thu, 08 Jan 2015 13:23:00 +0000 Dr. Jeff Lichtman https://www.leica-microsystems.com/11763 Basics in Microscopy Video Talk by Jeff Lichtman: Resolution in Microscopy – Wave Optics and the Diffraction Limit Light has properties of particles and waves. Understanding the wave nature of light is essential to understanding the workings of a microscope. This lecture describes Huygens Wavelets, constructive/destructive interference, and diffraction. https://www.leica-microsystems.com/science-lab/video-talk-by-jeff-lichtman-resolution-in-microscopy-wave-optics-and-the-diffraction-limit/ Thu, 14 Aug 2014 12:48:00 +0000 Dr. Jeff Lichtman