Leica Science Lab - Tag : Magnification https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=3&cHash=0fbcf1e4a27b1f5edd4b4fb7bf9d628a Article tagged with Magnification en-US https://www.leica-microsystems.com/5098 Stereo Microscopy Basics in Microscopy Quality Assurance Key Factors to Consider When Selecting a Stereo Microscope Stereo microscopes are often nicknamed the “workhorse” of the lab or production site. Users spend many hours looking through the eyepieces inspecting, observing, documenting, or dissecting samples. Careful assessment of the relevant applications for which the stereo microscope is needed is key to long-lasting, satisfactory use. https://www.leica-microsystems.com/science-lab/factors-to-consider-when-selecting-a-stereo-microscope/ Thu, 24 Sep 2020 07:00:00 +0000 B.Sc. Elisabeth Ippel, Daniel Goeggel, PhD James DeRose https://www.leica-microsystems.com/15127 Basics in Microscopy Digital Microscopy Stereo Microscopy What Does 30,000:1 Magnification Really Mean? One important criterion concerning the performance of an optical microscope is magnification. This report will offer digital microscopy users helpful guidelines to determine the useful range of magnification values. https://www.leica-microsystems.com/science-lab/what-does-300001-magnification-really-mean/ Mon, 25 Jun 2018 22:00:00 +0000 MSc Michael Doppler, PhD James DeRose https://www.leica-microsystems.com/19605 Basics in Microscopy Collecting Light: The Importance of Numerical Aperture in Microscopy Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA is a number without units and is related to the angles of light which are collected by a lens. In calculating NA (see below), the refractive index of a medium is also taken into account and by matching the refractive index of a slide or cell culture container with an immersion medium, then more of the detail of a specimen will be resolved. The way in which light behaves when travelling from one medium to another is also related to NA (and termed ‘refraction’). This article also covers a brief history of refraction and how this concept is a limiting factor in achieving high NA. https://www.leica-microsystems.com/science-lab/collecting-light-the-importance-of-numerical-aperture-in-microscopy/ Wed, 12 Jul 2017 07:46:00 +0000 PhD Martin Wilson https://www.leica-microsystems.com/18991 Basics in Microscopy Microscope Resolution: Concepts, Factors and Calculation In microscopy, the term ‘resolution’ is used to describe the ability of a microscope to distinguish detail. In other words, this is the minimum distance at which two distinct points of a specimen can still be seen - either by the observer or the microscope camera - as separate entities. The resolution of a microscope is intrinsically linked to the numerical aperture (NA) of the optical components as well as the wavelength of light which is used to examine a specimen. In addition, we have to consider the limit of diffraction which was first described in 1873 by Ernst Abbe. This article covers some of the history behind these concepts as well as explaining each using relatively simple terminology. https://www.leica-microsystems.com/science-lab/microscope-resolution-concepts-factors-and-calculation/ Fri, 02 Dec 2016 14:09:00 +0000 PhD Martin Wilson https://www.leica-microsystems.com/16124 Basics in Microscopy A Brief History of Light Microscopy – From the Medieval Reading Stone to Super-Resolution The history of microscopy begins in the Middle Ages. As far back as the 11th century, plano-convex lenses made of polished beryl were used in the Arab world as reading stones to magnify manuscripts. However, the further development of these lenses into the first microscopes cannot be attributed to any one person. It took the ideas and designs of many scientists and scholars to produce instruments capable of strong magnification. https://www.leica-microsystems.com/science-lab/a-brief-history-of-light-microscopy-from-the-medieval-reading-stone-to-super-resolution/ Tue, 08 Sep 2015 17:41:00 +0000 Wymke Ockenga https://www.leica-microsystems.com/11767 Digital Microscopy Using Telecentric Optical Systems to Optimize Industrial Image Accuracy and Reproducibility When the first multi-lens microscopes were invented in 1590, scientists marveled at their new ability to SEE small objects and features in the natural world that were previously invisible to the eye and therefore seemingly nonexistent. With the constant miniaturization of parts and products in automated manufacturing over the past 5 decades, the use of microscopes has spread increasingly from science to industry. Today microscopes are found in a multitude of assembly and inspection applications wherever visualization and measurement of miniscule features are required. https://www.leica-microsystems.com/science-lab/using-telecentric-optical-systems-to-optimize-industrial-image-accuracy-and-reproducibility/ Thu, 25 Jun 2015 18:57:00 +0000 Clinton Smith https://www.leica-microsystems.com/5988 Basics in Microscopy Optical Microscopes – Some Basics The optical microscope has been a standard tool in life science as well as material science for more than one and a half centuries now. To use this tool economically and effectively, it helps a lot to understand the basics of optics, especially of those essential components which are part of every microscope. https://www.leica-microsystems.com/science-lab/optical-microscopes-some-basics/ Wed, 02 May 2012 22:00:00 +0000 Helmut Rühl https://www.leica-microsystems.com/2558 Stereo Microscopy Basics in Microscopy Widefield Microscopy Digital Cameras Manufacturers digital cameras race to outdo each other with ever-increasing numbers of megapixels. The world record for professional medium format digital cameras has now surpassed 60 megapixels per shot using a very large and expensive sensor with a resolution of about 9000 x 6700 pixels. Each time you capture such an image you get about 180 MB of uncompressed data and even more if you switch to 16-bit per colour for full dynamic range. https://www.leica-microsystems.com/science-lab/digital-cameras/ Thu, 30 Apr 2009 22:00:00 +0000 Urs Schmid https://www.leica-microsystems.com/2472 Basics in Microscopy Widefield Microscopy Beware of "Empty" Magnification This article explains how to avoid the phenomen of "empty magnification" in microscopy. https://www.leica-microsystems.com/science-lab/beware-of-empty-magnification/ Sun, 18 May 2008 10:00:00 +0000 Dipl. oec.-troph. Anja Schué https://www.leica-microsystems.com/5350 Stereo Microscopy Quality Assurance FusionOptics – Combines high resolution and depth of field for ideal 3D optical Images A study carried out jointly by Leica Microsystems and the Institute of Neuroinformatics at the University of Zurich and Swiss Federal Institute of Technology provided the basis for an innovation in stereomicroscopy: FusionOptics™. The significant performance increase attained by FusionOptics™ is highly valuable for everyday work at the microscope. https://www.leica-microsystems.com/science-lab/fusionoptics-combines-high-resolution-and-depth-of-field-for-ideal-3d-optical-images/ Thu, 17 Apr 2008 22:00:00 +0000 Daniel Goeggel, Dipl. oec.-troph. Anja Schué, Dr. Daniel Kiper