Leica Science Lab - Tag : HyD https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=423&cHash=66a49615b0c3cd9eb21a651b64a2605c Article tagged with HyD en-US https://www.leica-microsystems.com/24781 Confocal Microscopy Neuroscience Zebrafish Brain - Whole Organ Imaging at High Resolution Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete brain dissected from a developing zebrafish, one would need to cover a field of some ten square millimeters at a depth in the millimeter range. Usually, low magnification lenses do not provide sufficient resolution to reveal the intricate structural interactions in nervous tissue. Additionally, due to scattering processes, the depth at which one can image within dense biological tissue using a confocal microscope is generally restricted to approximately 10 microns. https://www.leica-microsystems.com/science-lab/zebrafish-brain-whole-organ-imaging-at-high-resolution/ Mon, 08 Apr 2019 22:00:00 +0000 Dr. Rolf T. Borlinghaus, Ryan E. Robinson https://www.leica-microsystems.com/24711 Confocal Microscopy What is a Hybrid Detector (HyD)? A HyD is a sensor for detecting light (photons). It is a compound (hybrid) of two technologies: vacuum tubes, like those used in a photomultiplier tube (PMT), and semiconductor microelectronics, like an avalanche photodiode (APD). The Hybrid Detector combines essentially the advantages of both approaches. It is fast, shows very little noise, and offers a high dynamic range. For a comparison see references 1, 2, 3, and 4. The HyD is the best suited sensor for most confocal and multiphoton applications. https://www.leica-microsystems.com/science-lab/what-is-a-hybrid-detector-hyd/ Wed, 06 Mar 2019 23:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/20323 Confocal Microscopy Which Sensor is the Best for Confocal Imaging? The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article. https://www.leica-microsystems.com/science-lab/which-sensor-is-the-best-for-confocal-imaging/ Mon, 16 Jul 2018 22:00:00 +0000 Holger Birk, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/20109 Quantitative Imaging Lifetime – a Proper Alternative „Way too complicated!“ - the notorious feedback when it comes to fluorescence lifetime measurements. This will change now! New technologies and new concepts for data evaluation, all implemented in the new Leica SP8 FALCON, render fluorescence lifetime imaging (FLIM) as fuss-free as ordinary confocal imaging. And by the way: with Leica FALCON you can record frames 10 times faster compared to the classical standard. And three (or more) dimensional image stacks or time series are generated in a snap. Four channels simultaneously? No problem! And of course there are tunable excitation wavelength both visible with white light lasers (WLL) and infrared (the latter for multiphoton microscopy). That should be reason enough to delve into fluorescence lifetime imaging. The picture shows a lifetime image of a mouse embryo. Recorded in 722 stitched tiles and fitted for four separate characteristic times. Recording time ca 1 hour – compared to ca 1 day with the classical approach. https://www.leica-microsystems.com/science-lab/lifetime-a-proper-alternative/ Wed, 28 Mar 2018 22:00:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/18098 Super-Resolution Actin-Dependent Vacuolar Occupancy of the Cell Determines Auxin-Induced Growth Repression The cytoskeleton is an early attribute of cellular life, and its main components are composed of conserved proteins. The actin cytoskeleton has a direct impact on the control of cell size in animal cells, but its mechanistic contribution to cellular growth in plants remains largely elusive. Here, we reveal a role of actin in regulating cell size in plants. The actin cytoskeleton shows proximity to vacuoles, and the phytohormone auxin not only controls the organization of actin filaments but also impacts vacuolar morphogenesis in an actin-dependent manner. https://www.leica-microsystems.com/science-lab/actin-dependent-vacuolar-occupancy-of-the-cell-determines-auxin-induced-growth-repression/ Fri, 03 Feb 2017 08:14:00 +0000 https://www.leica-microsystems.com/18900 Super-Resolution Confocal Microscopy P53- and Mevalonate Pathway–Driven Malignancies Require Arf6 for Metastasis and Drug Resistance Application example of HvYolution Super-Resolution - Drug resistance, metastasis, and a mesenchymal transcriptional program are central features of aggressive breast tumors. The GTPase Arf6, often overexpressed in tumors, is critical to promote epithelial–mesenchymal transition and invasiveness. The metabolic mevalonate pathway (MVP) is associated with tumor invasiveness and known to prenylate proteins, but which prenylated proteins are critical for MVP-driven cancers is unknown. We show here that MVP requires the Arf6-dependent mesenchymal program. https://www.leica-microsystems.com/science-lab/p53-and-mevalonate-pathway-driven-malignancies-require-arf6-for-metastasis-and-drug-resistance/ Wed, 19 Oct 2016 16:27:00 +0000 https://www.leica-microsystems.com/16460 Confocal Microscopy Multiphoton Microscopy From Light to Mind: Sensors and Measuring Techniques in Confocal Microscopy This article outlines the most important sensors used in confocal microscopy. By confocal microscopy, we mean "True Confocal Scanning", i.e. the technique that illuminates and measures one single point only. The aim is not to impart in-depth specialist knowledge, but to give the user a small but clear overview of the differences between the various technologies and to advise on which sensor may be most suitable for which applications. https://www.leica-microsystems.com/science-lab/from-light-to-mind-sensors-and-measuring-techniques-in-confocal-microscopy/ Fri, 28 Aug 2015 16:25:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/15219 Confocal Microscopy "Leica is always flexible and dynamic" - Interview with Audrey Salles, Pasteur Institute, Paris Audrey Salles is a specialist for confocal and super-resolution microscopy at Pasteur Institute, Imagopole, PFID, Paris, France. Her research interests are cytokine signaling and skeleton organization of human TCD4-cells. https://www.leica-microsystems.com/science-lab/leica-is-always-flexible-and-dynamic-interview-with-audrey-salles-pasteur-institute-paris/ Mon, 12 Jan 2015 11:10:00 +0000 Audrey Salles, PhD Isabelle Köster, Dipl. oec.-troph. Anja Schué https://www.leica-microsystems.com/10111 Confocal Microscopy Multiple Microscopy Modes in a Single Sweep with Supercontinuum White Light Lasers have been critical to the advancement on confocal microscopy, and the white light laser (WLL) offers particular advantages. Finessing WLL output for bioimaging is a complex task, though, and traditional approaches retain key limitations. But acousto-optical beamsplitting enables smoother operation, leading to enhanced microscopy capabilities. https://www.leica-microsystems.com/science-lab/multiple-microscopy-modes-in-a-single-sweep-with-supercontinuum-white-light/ Fri, 14 Jun 2013 14:09:00 +0000 Dr. Lioba Kuschel, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/8119 Confocal Microscopy The White Confocal – Spectral Gaps Closed This article summarizes the development and differences in design and functionality of confocal technology as far as spectral properties are concerned, from classical filter-based excitation and emission color selection to fully flexible spectral excitation and emission tuning. All three major components: light source with excitation color selection, beam splitting for incident illumination and detector emission filtering have been completely transformed. https://www.leica-microsystems.com/science-lab/the-white-confocal-spectral-gaps-closed/ Sun, 24 Feb 2013 23:00:00 +0000 Dr. Lioba Kuschel, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/7988 Confocal Microscopy Super-Resolution Gates Open for Improved Confocal Fluorescence and Super-Resolution STED True confocal microscope systems feature single-point illumination and single-point detection. The method is called "optical sectioning" since the generated image contains only information from the focal plane. The serial detection offers highly efficient and low-noise sensors for signal conversion. Although the nonparallel detection is not conducive to high-speed imaging, modern scanning concepts allow frame rates above 400 frames per second at reasonable noise levels. This is by far enough for most applications, including the monitoring of fast ion-transport phenomena in living material. https://www.leica-microsystems.com/science-lab/gates-open-for-improved-confocal-fluorescence-and-super-resolution-sted/ Wed, 23 Jan 2013 23:00:00 +0000 Dr. Rolf T. Borlinghaus