Leica Science Lab - Tag : AOTF https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=447&cHash=b2428b16bbd6a9e34a5b0de3bba25ecd Article tagged with AOTF en-US https://www.leica-microsystems.com/11235 Confocal Microscopy Acousto Optics in True Confocal Spectral Microscope Systems Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this technique. Acousto-optical elements are highly transparent, quickly tunable and allow many colors to be managed simultaneously. As they show a strong dependence in polarization and have comparably small dimensions, their active part is used to modify and guide the laser illumination light, thereby leaving the principal beam (0th order) unaffected. Excitation color selection and attenuation (excitation filtering), as well as separation of illumination and detection light (beam splitting) are the main fields of application. https://www.leica-microsystems.com/science-lab/acousto-optics-in-true-confocal-spectral-microscope-systems/ Fri, 01 Nov 2013 05:47:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/6415 Confocal Microscopy White Light Laser The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence. Furthermore, it should offer means to avoid reflection of excitation light, and the coupling into the beam path must be efficient and homogeneous throughout the full visible spectrum. Such a source has been invented and implemented: the white light laser in combination with acousto-optical beam splitting. https://www.leica-microsystems.com/science-lab/white-light-laser/ Tue, 26 Jun 2012 22:00:00 +0000 Dr. Lioba Kuschel, Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/5945 TIRF Microscopy Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the evanescent wave, which arises from the glass/water or glass/specimen interface, is that its propagation in z-direction gradually degrades, limiting its penetration depth into the specimen to some hundred nanometers. https://www.leica-microsystems.com/science-lab/controlling-the-tirf-penetration-depth-is-mandatory-for-reproducible-results/ Tue, 24 Apr 2012 22:00:00 +0000 Dr. Thomas Veitinger https://www.leica-microsystems.com/4465 Confocal Microscopy Confocal Excitation Fluorescence excitation needs specifically colored light. In confocal microscopy, multiline lasers or laser batteries are classically used. This requires devices that pick the requested lines fitting the currently employed fluorochromes. Intensity control is a second task that must be accomplished. https://www.leica-microsystems.com/science-lab/confocal-excitation/ Fri, 09 Dec 2011 07:59:00 +0000 Dr. Rolf T. Borlinghaus