Leica Science Lab - Tag : Alexa Fluor Dyes https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=494&cHash=84a42000536eac7f283e62294fdaf9c5 Article tagged with Alexa Fluor Dyes en-US https://www.leica-microsystems.com/44724 Confocal Microscopy Fluorescence Microscopy A New World of Confocal Applications with the Next Generation White Light Lasers As biological questions get more complex, there is an increasing need to study multiple events simultaneously in the same specimen. When preparing the specimen for imaging experiments, this need is translated in having to use multiple fluorescent labels. Unfortunately, the visualization of multiple events with confocal microscopy is limited by the number of fluorescent probes that can be simultaneously imaged without incurring bleed-through artifacts due to significant spectral overlap. Using a confocal microscope with an integrated White Light Laser (WLL) enables tuning excitation and detection to perfectly match the spectral profile of the dyes in your sample, giving you the flexibility to perform multicolor experiments while obtaining clear, sharp images. https://www.leica-microsystems.com/science-lab/a-new-world-of-confocal-applications-with-the-next-generation-white-light-lasers/ Thu, 03 Dec 2020 14:20:00 +0000 https://www.leica-microsystems.com/6345 Fluorescence Microscopy Widefield Microscopy Fluorescent Dyes A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescing agent. This can be a fluorescing protein – for example GFP – genetically linked to the protein of interest. If cloning is impossible – for instance in histologic samples – it is required to use other techniques like immunofluorescence staining to visualize the protein of interest. https://www.leica-microsystems.com/science-lab/fluorescent-dyes/ Sun, 10 Jun 2012 22:00:00 +0000 Dr. Christoph Greb