Leica Science Lab - Tag : Calcium Imaging https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=525&cHash=575cc3c912a4adf9195e4da885170aee Article tagged with Calcium Imaging en-US https://www.leica-microsystems.com/16593 Super-Resolution Neuroscience Super-Resolution Microscopy of the Synaptic Active Zone At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Recently, super-resolution microscopy (SRM) techniques have begun to enter the neurosciences. These approaches combine high spatial resolution with the molecular specificity of fluorescence microscopy. Here, we discuss how SRM techniques can be used to obtain information on the organization of AZ proteins. https://www.leica-microsystems.com/science-lab/super-resolution-microscopy-of-the-synaptic-active-zone/ Tue, 15 Dec 2015 10:15:00 +0000 Nadine Ehmann https://www.leica-microsystems.com/15426 Confocal Microscopy Progressive Glucose Stimulation of Islet Beta Cells Reveals a Transition From Segregated to Integrated Modular Functional Connectivity Patterns Collective beta cell activity in islets of Langerhans is critical for the supply of insulin within an organism. In order to get a detailed insight into the functional organization of the syncytium, we applied advanced analytical tools from the realm of complex network theory to uncover the functional connectivity pattern among cells composing the intact islet. https://www.leica-microsystems.com/science-lab/progressive-glucose-stimulation-of-islet-beta-cells-reveals-a-transition-from-segregated-to-integrated-modular-functional-connectivity-patterns/ Thu, 03 Sep 2015 17:10:00 +0000 https://www.leica-microsystems.com/14063 Confocal Microscopy Multiphoton Microscopy Smart Control for Resonant Galvo Scanners High time-resolution confocal microscopy (HTRCLSM) requires fast scanning devices. Whereas non-resonant galvo scanners allow full position control, but only at slow speed, resonant scanners allow ~25,000 lines per second, but offer much less positioning freedom. To still allow zoom and pan functions, several approaches have been tried, with varying success. The Leica confocal microscopes od the TCS series use a very smart solution that enables stepless zooming with short switching times. https://www.leica-microsystems.com/science-lab/smart-control-for-resonant-galvo-scanners/ Fri, 01 Aug 2014 08:29:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/11000 Confocal Microscopy Neuroscience Live-Cell Imaging Imaging Pheromone Sensing in a Mouse Vomeronasal Acute Tissue Slice Preparation In mice, the ability to detect pheromones is principally mediated by the vomeronasal organ (VNO). Here, an acute tissue slice preparation of VNO for performing calcium imaging is described. This physiological approach allows observations of subpopulations and/or individual neurons in a living tissue and is convenient for receptor-ligand identification. https://www.leica-microsystems.com/science-lab/imaging-pheromone-sensing-in-a-mouse-vomeronasal-acute-tissue-slice-preparation/ Fri, 02 May 2014 14:24:00 +0000 https://www.leica-microsystems.com/12418 Confocal Microscopy Live-Cell Imaging Glucose-Stimulated Calcium Dynamics in Islets of Langerhans in Acute Mouse Pancreas Tissue Slices In endocrine cells within islets of Langerhans calcium ions couple cell stimulation to hormone secretion. Since the advent of modern fluorimetry, numerous in vitro studies employing primarily isolated mouse islets have investigated the effects of various secretagogues on cytoplasmic calcium, predominantly in insulin-secreting beta cells. Due to technical limitations, insights of these studies are inherently limited to a rather small subpopulation of outermost cells. https://www.leica-microsystems.com/science-lab/glucose-stimulated-calcium-dynamics-in-islets-of-langerhans-in-acute-mouse-pancreas-tissue-slices/ Tue, 11 Mar 2014 07:00:00 +0000 https://www.leica-microsystems.com/12420 Confocal Microscopy Live-Cell Imaging Functional Connectivity in Islets of Langerhans from Mouse Pancreas Tissue Slices We propose a network representation of electrically coupled beta cells in islets of Langerhans. Beta cells are functionally connected on the basis of correlations between calcium dynamics of individual cells, obtained by means of confocal laser-scanning calcium imaging in islets from acute mouse pancreas tissue slices. https://www.leica-microsystems.com/science-lab/functional-connectivity-in-islets-of-langerhans-from-mouse-pancreas-tissue-slices/ Mon, 10 Mar 2014 10:31:00 +0000 https://www.leica-microsystems.com/12002 Confocal Microscopy Live-Cell Imaging The Relationship Between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta cells are associated with in-phase oscillatory cytosolic calcium activity ([Ca2+]i) measured with fluorescent probes. Recent high spatial and temporal resolution confocal imaging revealed that glucose stimulation of beta cells in intact islets within acute tissue slices produces a [Ca2+]i change with initial transient phase followed by a plateau phase with highly synchronized [Ca2+]i oscillations. https://www.leica-microsystems.com/science-lab/the-relationship-between-membrane-potential-and-calcium-dynamics-in-glucose-stimulated-beta-cell-syncytium-in-acute-mouse-pancreas-tissue-slices/ Tue, 17 Dec 2013 17:08:00 +0000 https://www.leica-microsystems.com/6656 Live-Cell Imaging Quantitative Imaging Widefield Calcium Imaging with Calcium Indicator Fura2 In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are usually kept low, as Ca2+ often forms insoluble complexes with phosphorylated and carboxylated compounds. Typically cytosolic Ca2+ concentrations are in the range of 100 nM. In response to stimuli Ca2+ may either be released from external medium or internal stores to raise the Ca2+ concentration. https://www.leica-microsystems.com/science-lab/widefield-calcium-imaging-with-calcium-indicator-fura2/ Sun, 13 Jan 2013 23:00:00 +0000 Gabriele Burger, Simone Diehl https://www.leica-microsystems.com/4718 Neuroscience Neurobiology and Microscopy Neurobiology, the science of nerves and the brain, has mainly been driven forward in the last 200 years by microscopic investigations. The structures of cellular and subcellular structures, interaction and the three-dimensional assembly of neurons were made visible by various microscopy techniques. The optical microscope is also a necessary tool for visualizing micropipettes in electrophysiological measurements. Thirdly, many types of functional imaging are performed by means of optical microscopy. https://www.leica-microsystems.com/science-lab/neurobiology-and-microscopy/ Wed, 09 Nov 2011 13:07:00 +0000 Dr. Rolf T. Borlinghaus