Leica Science Lab - Tag : Dichroic Mirror https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=625&cHash=711749cc6e1518eceb46f708cd9d7e33 Article tagged with Dichroic Mirror en-US https://www.leica-microsystems.com/19260 Basics in Microscopy Fluorescence Microscopy Milestones in Incident Light Fluorescence Microscopy Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the help of fluorescence molecules is a standard method in nearly every life science discipline today. This broad application range goes back to the technical work of some researchers who wanted to improve and simplify fluorescence microscopic labor. One person who was involved in that development was the Dutch medic Johann Sebastiaan Ploem. https://www.leica-microsystems.com/science-lab/milestones-in-incident-light-fluorescence-microscopy/ Mon, 06 Mar 2017 08:39:00 +0000 Dr. Christoph Greb https://www.leica-microsystems.com/11235 Confocal Microscopy Acousto Optics in True Confocal Spectral Microscope Systems Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this technique. Acousto-optical elements are highly transparent, quickly tunable and allow many colors to be managed simultaneously. As they show a strong dependence in polarization and have comparably small dimensions, their active part is used to modify and guide the laser illumination light, thereby leaving the principal beam (0th order) unaffected. Excitation color selection and attenuation (excitation filtering), as well as separation of illumination and detection light (beam splitting) are the main fields of application. https://www.leica-microsystems.com/science-lab/acousto-optics-in-true-confocal-spectral-microscope-systems/ Fri, 01 Nov 2013 05:47:00 +0000 Dr. Rolf T. Borlinghaus https://www.leica-microsystems.com/9350 Fluorescence Microscopy Confocal Microscopy Live-Cell Imaging Handbook of Optical Filters for Fluorescence Microscopy Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and an optic that might be appropriate and optimal for one is both inappropriate and sub-optimal for another. https://www.leica-microsystems.com/science-lab/handbook-of-optical-filters-for-fluorescence-microscopy/ Tue, 06 Aug 2013 07:59:00 +0000 BSc. Jay Reichman https://www.leica-microsystems.com/4598 Confocal Microscopy Spectral Imaging To separate fractions of the emission for recording channels that reproduce the emission of individual fluorochromes, it is necessary to spatially disperse the emission spectrally. This is possible by employing dichroic mirrors or a genuine dispersive element like a prism or a grating. https://www.leica-microsystems.com/science-lab/spectral-imaging/ Fri, 09 Dec 2011 10:20:00 +0000 Dr. Rolf T. Borlinghaus