Leica Science Lab - Tag : AOBS https://www.leica-microsystems.com//science-lab/tag/aobs/ Article tagged with AOBS en-US https://www.leica-microsystems.com/19283 Confocal Microscopy Primary Beam Splitting Devices for Confocal Microscopes Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam splitting mirror which has fixed spectral parameters and transmits the emission usually between 90% and 98% within the designated bands. Transmission is wavelength dependent and also differs by technology, requirements and design. An alternative is the acousto optical beam splitter which has freely tunable reflection notches and transmits the emission on average at 95% between these notches. https://www.leica-microsystems.com/science-lab/primary-beam-splitting-devices-for-confocal-microscopes/ Tue, 16 May 2017 07:42:00 +0000 https://www.leica-microsystems.com/15200 Confocal Microscopy Video: The White Light Laser – How to Effectively Excite Multiple Fluorophores with a Single Light Source The Leica White Light Laser produces a continuous spectral output between the wavelengths of 470 and 670 nm. It allows you to select 8 excitation lines from 3 trillion unique combinations for simultaneous imaging of multiple fluorophores. The white light laser source of the Leica TCS SP8 X perfectly matches the wavelength of any fluorophore. https://www.leica-microsystems.com/science-lab/video-the-white-light-laser-how-to-effectively-excite-multiple-fluorophores-with-a-single-light-source/ Thu, 27 Aug 2015 10:05:00 +0000 PhD Christopher Vega https://www.leica-microsystems.com/14656 Confocal Microscopy Super-Resolution CLEM Live-Cell Imaging Quantitative Imaging ICln: A New Regulator of Non-Erythroid 4.1R Localisation and Function To optimise the efficiency of cell machinery, cells can use the same protein (often called a hub protein) to participate in different cell functions by simply changing its target molecules. There are large data sets describing protein-protein interactions ("interactome") but they frequently fail to consider the functional significance of the interactions themselves. https://www.leica-microsystems.com/science-lab/icln-a-new-regulator-of-non-erythroid-41r-localisation-and-function/ Mon, 03 Nov 2014 18:02:00 +0000 https://www.leica-microsystems.com/11235 Confocal Microscopy Acousto Optics in True Confocal Spectral Microscope Systems Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this technique. Acousto-optical elements are highly transparent, quickly tunable and allow many colors to be managed simultaneously. As they show a strong dependence in polarization and have comparably small dimensions, their active part is used to modify and guide the laser illumination light, thereby leaving the principal beam (0th order) unaffected. Excitation color selection and attenuation (excitation filtering), as well as separation of illumination and detection light (beam splitting) are the main fields of application. https://www.leica-microsystems.com/science-lab/acousto-optics-in-true-confocal-spectral-microscope-systems/ Fri, 01 Nov 2013 05:47:00 +0000 https://www.leica-microsystems.com/10111 Confocal Microscopy Multiple Microscopy Modes in a Single Sweep with Supercontinuum White Light Lasers have been critical to the advancement on confocal microscopy, and the white light laser (WLL) offers particular advantages. Finessing WLL output for bioimaging is a complex task, though, and traditional approaches retain key limitations. But acousto-optical beamsplitting enables smoother operation, leading to enhanced microscopy capabilities. https://www.leica-microsystems.com/science-lab/multiple-microscopy-modes-in-a-single-sweep-with-supercontinuum-white-light/ Fri, 14 Jun 2013 14:09:00 +0000 Dr. Lioba Kuschel https://www.leica-microsystems.com/9421 Confocal Microscopy The First Supercontinuum Confocal that Adapts to the Sample Until now, biological and medical research fluorescence imaging in multi-user facilities or institutes has been limited by the type or number of dyes that could be excited. The Leica TCS SP5 X supercontinuum confocal unites the broadband capabilities of the Leica TCS SP5 AOBS® and the freedom and flexibility to select any excitation line within the continuous range of 470 to 670 nm. https://www.leica-microsystems.com/science-lab/the-first-supercontinuum-confocal-that-adapts-to-the-sample/ Fri, 22 Aug 2008 16:40:00 +0000 PhD Kees Jalink, Prof. Alberto Diaspro, Valentina Caorsi, Paolo Bianchini, Scott Young