Leica Science Lab - Tag : Glass Knife https://www.leica-microsystems.com//science-lab/tag/?tx_leicaacademy_pi4%5Baction%5D=show&tx_leicaacademy_pi4%5Bcontroller%5D=Tag&tx_leicaacademy_pi4%5Btag%5D=696&cHash=96f85d36e5b1a2649d65a887a28290b6 Article tagged with Glass Knife en-US https://www.leica-microsystems.com/14345 EM Sample Preparation Introduction to Ultramicrotomy When studying samples, to visualize their fine structure with nanometer scale resolution, most often electron microscopy is used. There are 2 types: scanning electron microscopy (SEM) which images the sample surface or transmission electron microscopy (TEM) which requires a very thin, electron-transparent sample. Thus, to image the fine structure inside a sample using electron microscopy, the solution is to make very thin sections of it. https://www.leica-microsystems.com/science-lab/brief-introduction-to-ultramicrotomy/ Tue, 17 Sep 2019 09:06:00 +0000 Robert Ranner, PhD James DeRose https://www.leica-microsystems.com/10224 EM Sample Preparation Brief Introduction to Glass Knifemaking for Electron and Light Microscope Applications Glass knives are used in an ultramicrotome to cut ultrathin slices of samples for electron and light microscope applications. For resin and for cryo sections (Tokuyasu samples) the knife edge must be extremely sharp, strong and stable. An important requirement for breaking glass knives of superior quality is the quality of the glass strips. Glass strips are produced from specially selected glass, the thickness and quality of which is precisely controlled. Only strict tolerances ensured by careful quality control allow breaking of two high quality knives from one square. https://www.leica-microsystems.com/science-lab/brief-introduction-to-glass-knifemaking-for-electron-and-light-microscope-applications/ Fri, 13 Sep 2013 15:49:00 +0000 Dr. Ruwin Pandithage https://www.leica-microsystems.com/11027 EM Sample Preparation Neuroscience Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron microscope (FIB/SEM). The samples are fixed with aldehydes, heavy metal stained using osmium tetroxide and uranyl acetate. They are then dehydrated with alcohol and infiltrated with resin, which is then hardened. https://www.leica-microsystems.com/science-lab/focussed-ion-beam-milling-and-scanning-electron-microscopy-of-brain-tissue/ Wed, 06 Jul 2011 16:17:00 +0000