Leica Science Lab - Tag : Light Sheet Microscopy https://www.leica-microsystems.com//science-lab/tag/light-sheet-microscopy/ Article tagged with Light Sheet Microscopy en-US https://www.leica-microsystems.com/71608 Light Sheet Microscopy Understanding Motor Sequence Generation Across Spatiotemporal Scales We have developed a microscopy-based pipeline to characterize a developmentally critical behavior at the pupal stage of development, called the ecdysis sequence. We study brain-wide neuronal activity with a custom light-sheet microscope, whole-organism behavior at single muscle-fiber resolution and bridge the gap with simultaneous dual-color imaging of muscle activity with sensory neuron or motor neuron activity. These tools allow us to determine the roles of specific populations of neurons in generating motor output. https://www.leica-microsystems.com/understanding-motor-sequence-generation-across-spatiotemporal-scales/ Thu, 15 Apr 2021 11:49:00 +0000 Corporate Communications https://www.leica-microsystems.com/72012 Light Sheet Microscopy Confocal Microscopy Quantitative Imaging A Quality Metric for the Systematic Evaluation of Clearing Protocols 3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they mimic the in vivo tumor environment better than standard 2D cell cultures. However, despite the biological advantages of spheroids, visualizing single cells in the innermost layers of large 3D spheroids with current microscope solutions remains a challenge, mainly due to light scattering causing poor penetration. https://www.leica-microsystems.com/science-lab/a-quality-metric-and-light-sheet-imaging-guideline/ Thu, 15 Apr 2021 11:25:00 +0000 https://www.leica-microsystems.com/72008 Light Sheet Microscopy 3D in vivo light-sheet imaging of zebrafish brain activity using microfluidics The zebrafish larval brain is small and contains about 1 million times less neurons than the human counterpart. However, the neural circuitry and the general bauplan of the vertebrate brain is evolutionary conserved. Moreover, zebrafish larvae are optically translucent offering a unique possibility to investigate neural activity throughout the whole brain in real time with advanced imaging techniques, such as light-sheet microscopy. Microfluidics, on the other hand, allow the application of water soluble chemical stimuli with high spatiotemporal precision, including 3D focusing and concentration gradients. https://www.leica-microsystems.com/science-lab/3d-in-vivo-light-sheet-imaging-of-zebrafish-brain-activity-using-microfluidics/ Wed, 14 Apr 2021 09:14:00 +0000 https://www.leica-microsystems.com/24656 Light Sheet Microscopy Using Mounting Frames for DLS Sample Preparation Sample handling is an important topic in the context of Light Sheet Microscopy. The TCS SP8 DLS integrates Light Sheet technology into an inverted confocal platform and can hence make use of general principles concerning sample mounting and xy-stage functionality. We describe a set of mounting frames that allow preparation of a higher number of samples, also in the context of potentially harmful mounting media like BABB. https://www.leica-microsystems.com/science-lab/using-mounting-frames-for-dls-sample-preparation/ Sun, 10 Feb 2019 23:00:00 +0000 Simone Diehl, Dr. Petra Haas https://www.leica-microsystems.com/20488 Light Sheet Microscopy Using a Rotation Device for DLS Sample Mounting The TCS SP8 DLS from Leica Microsystems is an innovative concept to integrate the Light Sheet Microscopy technology into the confocal microscope. Due to its unique optical architecture samples can be mounted on standard glass bottom petri dishes and require only little adaptations when compared to conventional mounting procedures, as for confocal imaging. https://www.leica-microsystems.com/science-lab/using-a-rotation-device-for-dls-sample-mounting/ Tue, 22 Jan 2019 23:00:00 +0000 Dr. Petra Haas, Elena Remacha Motta, Laia Ortiz Lopez, Dr. Bruno Cossermelli Vellutini, Dr. Pavel Tomancak, Dr. Emmanuel Reynaud https://www.leica-microsystems.com/19710 Light Sheet Microscopy DLS Sample Preparation: Using U-Shaped Glass Capillaries for Sample Mounting The TCS SP8 DLS microscope system from Leica Microsystems is an innovative concept which integrates the Light Sheet Microscopy technology into the SP8 confocal platform. Due to its unique optical architecture, samples can be mounted on standard glass bottom petri dishes and require little or no adaptation when compared to conventional mounting procedures. Here, we present a convenient way to prepare specimens quickly for light sheet imaging with the TCS SP8 DLS system. https://www.leica-microsystems.com/science-lab/dls-sample-preparation-using-u-shaped-glass-capillaries-for-sample-mounting/ Wed, 06 Dec 2017 08:45:00 +0000 Dr. Petra Haas https://www.leica-microsystems.com/18796 Confocal Microscopy Multiphoton Microscopy Light Sheet Microscopy Neuroscience Clearing of Fixed Tissue: A Review from a Microscopist’s Perspective Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing interest in this field has both triggered and been stimulated by recent advances in high-throughput microscopy and data analysis methods, which allowed imaging and management of large samples. https://www.leica-microsystems.com/science-lab/clearing-of-fixed-tissue-a-review-from-a-microscopists-perspective/ Mon, 12 Sep 2016 11:20:00 +0000 https://www.leica-microsystems.com/16587 Confocal Microscopy Light Sheet Microscopy Live-Cell Imaging Light Sheet Microscopy Turned Vertically Living cells and organisms often suffer from the high light intensities used for fluorescent imaging. Light sheet microscopy reduces phototoxic effects and bleaching by illuminating a specimen in only a single plane at a time. A new light sheet microscope combines light sheet and confocal microscopy in one system without compromising either functionality and allows the combination of the two methods, e.g. confocal photomanipulation with subsequent light sheet acquisition, for new applications. https://www.leica-microsystems.com/science-lab/light-sheet-microscopy-turned-vertically/ Fri, 02 Oct 2015 13:34:00 +0000 PhD Isabelle Köster, Dr. Petra Haas https://www.leica-microsystems.com/16458 Confocal Microscopy Light Sheet Microscopy Super-Resolution "The Leica Digital Light Sheet Module – a Clever Example of Thinking Out of the Box" Bram van den Broek is a postdoctoral fellow at the Netherlands cancer institute in Amsterdam where he supports the advanced microscopy techniques in the laboratory of Kees Jalink. Working with Leica Microsystems as a collaboration partner for beta-testing of microscopes he enjoys very much. https://www.leica-microsystems.com/science-lab/the-leica-digital-light-sheet-module-a-clever-example-of-thinking-out-of-the-box/ Tue, 29 Sep 2015 14:39:00 +0000 Bram van den Broek, Dipl. oec.-troph. Anja Schué, PhD Isabelle Köster https://www.leica-microsystems.com/15606 Confocal Microscopy Light Sheet Microscopy Confocal and Digital Light Sheet Imaging Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the limited depth of field. Our eye-lens (an optical imaging instrument) has the same trouble, but our brain smartly removes all not-in-focus information before the signal reaches conscious cognition. https://www.leica-microsystems.com/science-lab/confocal-and-digital-light-sheet-imaging/ Mon, 11 May 2015 07:15:00 +0000 Dr. Rolf T. Borlinghaus, Dr. Petra Haas https://www.leica-microsystems.com/15077 Light Sheet Microscopy Nature Methods: Light-sheet Fluorescence Microscopy - Method of the Year 2014 Just about everyone who has examined fluorescent samples under the microscope is aware of the constant struggle to have enough signal to see the labeled structures while also avoiding fluorophore bleaching. What may be less apparent, at least to those who image bright, robust or fixed samples, is how stressful and potentially toxic to living cells and tissues it is to illuminate them with high-intensity light. https://www.leica-microsystems.com/science-lab/nature-methods-light-sheet-fluorescence-microscopy-method-of-the-year-2014/ Wed, 14 Jan 2015 17:24:00 +0000 https://www.leica-microsystems.com/11693 Confocal Microscopy Live-Cell Imaging Super-Resolution Neuroscience Fluorescence Microscopy Live-Cell Imaging Evolves to Find New Niches Since its introduction in the 1600s, improvements in microscope technology have continually broadened the types of cells and cellular processes that can be studied. Advances in automation have made this already-simple tool faster and more capable, and time-lapse imaging reveals function and dynamics in addition to structure. Live-cell imaging has enabled us to witness incredible moments in biology in unprecedented detail. Even embryogenesis – the process of cell division and cellular differentiation that occurs at the earliest stages of life – has recently been captured. https://www.leica-microsystems.com/science-lab/live-cell-imaging-evolves-to-find-new-niches/ Mon, 25 Nov 2013 10:15:00 +0000 https://www.leica-microsystems.com/15675 Light Sheet Microscopy Light Sheet Fluorescence Microscopy: Beyond the Flatlands Light Sheet Fluorescence Microscopy (LISH-M) is a true fluorescence optical sectioning technique, first described by Heinrich Siedentopf in 1902 under the name of Ultramicroscopy. Light sheet microscopy utilises a plane of light to optically section samples. This allows deep imaging within transparent tissues and whole organisms. This book chapter will provide the reader with a comprehensive view on this emerging technology. https://www.leica-microsystems.com/science-lab/light-sheet-fluorescence-microscopy-beyond-the-flatlands/ Thu, 20 Dec 2012 14:05:00 +0000 https://www.leica-microsystems.com/15671 Light Sheet Microscopy Quantitative Imaging in Cell Biology: Light Sheet Microscopy This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample rotation. https://www.leica-microsystems.com/science-lab/quantitative-imaging-in-cell-biology-light-sheet-microscopy/ Sun, 20 Nov 2011 13:30:00 +0000 https://www.leica-microsystems.com/15669 Light Sheet Microscopy Light Sheet Fluorescence Microscopy - A Review Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light, specimen photobleaching and phototoxicity are minimized compared to wide-field fluorescence, confocal, or multiphoton microscopy. https://www.leica-microsystems.com/science-lab/light-sheet-fluorescence-microscopy-a-review/ Sun, 20 Feb 2011 12:47:00 +0000 https://www.leica-microsystems.com/15667 Light Sheet Microscopy Selective Plane Illumination Microscopy Techniques in Developmental Biology Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. https://www.leica-microsystems.com/science-lab/selective-plane-illumination-microscopy-techniques-in-developmental-biology/ Mon, 15 Jun 2009 11:27:00 +0000