Leica Science Lab - Tag : Cryo SEM https://www.leica-microsystems.com//science-lab/tag/cryo-sem/ Article tagged with Cryo SEM en-US https://www.leica-microsystems.com/71639 CLEM Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is assessed and the distribution of target structures analyzed. It is shown how cryo confocal 3D data is projected on SEM images for improving the reliability of retaining the structure of interest within the final FIB lamella for further investigation in the cryo TEM. https://www.leica-microsystems.com/science-lab/targeting-active-recycling-nuclear-pore-complexes-using-cryo-confocal-microscopy/ Wed, 31 Mar 2021 06:09:00 +0000 https://www.leica-microsystems.com/30973 EM Sample Preparation Coronavirus Replication in Host Cells In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in replication, was visualized. https://www.leica-microsystems.com/science-lab/coronavirus-replication-in-host-cells/ Mon, 14 Sep 2020 12:45:00 +0000 PhD James DeRose https://www.leica-microsystems.com/26685 EM Sample Preparation Studying the Microstructure of Natural Polymers in Fine Detail The potential of cryogenic broad ion beam milling used in combination with scanning electron microscopy (cryo-BIB-SEM) for imaging and analyzing the microstructure of cryogenically stabilized soft polymers is assessed in this report. Results from the examination of delicate, natural polymers, such as tomato skin and wood, with cryo-BIB-SEM are shown. The polymer surface morphology and various microstructural features were analyzed. https://www.leica-microsystems.com/science-lab/studying-the-microstructure-of-natural-polymers-in-fine-detail/ Wed, 16 Oct 2019 10:30:00 +0000 PhD Joyce Schmatz, PhD James DeRose, PhD David R. Barbero, M.Sc. Martin Nopens https://www.leica-microsystems.com/25036 EM Sample Preparation Expert Knowledge on High Pressure Freezing and Freeze Fracturing in the Cryo SEM Workflow Get an insight in the working methods of the laboratory and learn about the advantages of Cryo SEM investigation in EM Sample Preparation. Find out how high pressure freezing, freeze fracturing and cryo transfer add to the Cryo SEM workflow and how the Leica portfolio ensures the compatibility between these different steps. https://www.leica-microsystems.com/science-lab/expert-knowledge-on-high-pressure-freezing-and-freeze-fracturing-in-the-cryo-sem-workflow/ Thu, 23 May 2019 22:00:00 +0000 Gisela Höflinger https://www.leica-microsystems.com/24707 EM Sample Preparation Cryo-SEM analysis of UV light stimulated sun screen lotion The EM ICE is the only available high-pressure freezer combining light stimulation with fast cryo fixation, a module mostly used for optogenetic approaches in neurobiology studies. However, this approach can be also very interesting for pharmaceutical or cosmetic industries to study the influence of UV light exposure on their target structures. This article shows an example on how UV light exposure changes the ultrastructure of sun screen lotion. https://www.leica-microsystems.com/science-lab/cryo-sem-analysis-of-uv-light-stimulated-sun-screen-lotion/ Wed, 06 Mar 2019 23:00:00 +0000 PhD Dietmar Pum, PhD Saskia Mimietz-Oeckler https://www.leica-microsystems.com/19176 EM Sample Preparation Drosophila larvae - Sample Preparation for Cryo-SEM Application Note for Leica EM ACE900 - Drosophila larvae were sandwiched between two 3 mm aluminum slit carriers with the 100 μm cavities facing each other and high-pressure frozen with a Leica EM HPM100. No ethanol as synchronization media was used, 1-hexadecene was used as filler. The wholes of the slit carriers were filled with filter tips dipped in 1-hexadecene to keep the carrier sandwich complete after freezing. https://www.leica-microsystems.com/science-lab/drosophila-larvae-sample-preparation-for-cryo-sem/ Mon, 30 Jan 2017 09:50:00 +0000 Dr. Andres Kaech, Prof. Damian Brunner https://www.leica-microsystems.com/19179 EM Sample Preparation Giardia lamblia - Sample Preparation for Cryo-SEM Application Note for Leica EM ACE900 - A 100 mesh copper grid (12 um thickness) was dipped into a concentrated Giardia suspension and sandwiched between two flat 3 mm aluminum specimen carriers with scratched surfaces. Subsequently, the sandwich was transferred to the widened hole of a middle plate (3.1 mm diameter). A 50 um spacer ring was added on top and the specimen immediately frozen with an HPM100 high-pressure freezing machine without using alcohol as synchronization fluid. https://www.leica-microsystems.com/science-lab/giardia-lamblia-sample-preparation-for-cryo-sem/ Mon, 30 Jan 2017 09:35:00 +0000 Dr. Andres Kaech, Joe Paulin Zumthor https://www.leica-microsystems.com/18312 EM Sample Preparation Commercially Available Hand Creams - Sample Preparation for Cryo-SEM Application Note for Leica EM HPM100 - Hand creams having different water contents were applied into the 100 μm cavities of two 3 mm type A sample carriers which were then closed cream sides inwards. The sample assembly was high pressure frozen with a Leica EM HPM100 and moved to a cooled Leica EM VCT100 loading station. https://www.leica-microsystems.com/science-lab/commercially-available-hand-creams-sample-preparation-for-cryo-sem/ Fri, 18 Nov 2016 17:22:00 +0000 Dr. Kim Rensing https://www.leica-microsystems.com/18284 EM Sample Preparation Cryo-SEM Imaging of Latex Paint Application Note for Leica EM VCT100, Leica EM ACE600 - A thin layer of latex paint was spread on a clean, scored, silicon chip. The sample was immediately plunge frozen in liquid ethane and transferred under LN2 to the Leica EM VCT100 loading station where it was placed in the customized sample holder. https://www.leica-microsystems.com/science-lab/cryo-sem-imaging-of-latex-paint/ Thu, 16 Jun 2016 16:50:00 +0000 Dr. Levi Felts, Dr. Kim Rensing, Chris Frethem https://www.leica-microsystems.com/17379 EM Sample Preparation High Pressure Freezing with Light Stimulation Sun screen lotion was carefully filled in the 100 μm incision of a 3 mm copper/gold plated flat carrier and covered with 3 mm sapphire disk. The sun screen lotion sample was then high pressure frozen with a Leica EM ICE with and subsequently without light stimulation. The light stimulated samples were exposed to a UV light for 500 milliseconds. https://www.leica-microsystems.com/science-lab/high-pressure-freezing-with-light-stimulation/ Fri, 18 Dec 2015 12:01:00 +0000 PhD Dietmar Pum, Dr. Cveta Tomova, PhD Saskia Mimietz-Oeckler https://www.leica-microsystems.com/14406 EM Sample Preparation Brief Introduction to Freeze Fracture and Etching Freeze fracture describes the technique of breaking a frozen specimen to reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured face that were originally hidden. A metal/carbon mix enables the sample to be imaged in a SEM (block-face) or TEM (replica). It is used to investigate for instance cell organelles, membranes, layers and emulsions. https://www.leica-microsystems.com/science-lab/brief-introduction-to-freeze-fracture-and-etching/ Wed, 01 Oct 2014 09:27:00 +0000 Gisela Höflinger