gated STED
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Time-resolved STED microscopy
Introduced more than 30 years ago, stimulated emission depletion (STED) microscopy has raised to a standard and widely used method for imaging in the life sciences. Thanks to continuous technological…Read article
The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS SP8 STED 3X nanoscope from Leica Microsystems. It…Read article
Actin-Dependent Vacuolar Occupancy of the Cell Determines Auxin-Induced Growth Repression
The cytoskeleton is an early attribute of cellular life, and its main components are composed of conserved proteins. The actin cytoskeleton has a direct impact on the control of cell size in animal…Read article
Translation Microscopy (TRAM) for Super-Resolution Imaging
Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We…Read article
STED-FLCS: An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics
Heterogeneous diffusion dynamics of molecules play an important role in many cellular signaling events, such as of lipids in plasma membrane bioactivity. However, these dynamics can often only be…Read article
Two-Photon Excitation STED Microscopy with Time-Gated Detection
We report on a novel two-photon excitation stimulated emission depletion (2PE-STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the…Read article
TFG Promotes Organization of Transitional ER and Efficient Collagen Secretion
Collagen is the most abundant protein in the animal kingdom. It is of fundamental importance during development for cell differentiation and tissue morphogenesis as well as in pathological processes…Read article
Gated STED Microscopy with Time-gated Single-photon Avalanche Diode
The maximization of the useful (within the time gate) photon flux is then an important aspect to obtain super-resolved STED images. Here we show that by using a fast-gated single-photon avalanche…Read article
A Straightforward Approach for Gated STED-FCS to Investigate Lipid Membrane Dynamics
Recent years have seen the development of multiple technologies to investigate, with great spatial and temporal resolution, the dynamics of lipids in cellular and model membranes. One of these…Read article
STED Nanoscopy: A Glimpse into the Future
The well-known saying of "Seeing is believing" became even more apt in biology when stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel laureate S. Hell and coworkers.…Read article
Pathways to Optical STED Microscopy
STED nanoscopy has evolved to a highly versatile tool for the observation of the living cell, more and more finding its way into state-of-the-art optical imaging facilities in biomedical research…Read article
Multi-Images Deconvolution Improves Signal-to-Noise Ratio on Gated Stimulated Emission Depletion Microscopy
Time-gated detection, namely, only collecting the fluorescence photons after a time-delay from the excitation events, reduces complexity, cost, and illumination intensity of a stimulated emission…Read article
ICln: A New Regulator of Non-Erythroid 4.1R Localisation and Function
To optimise the efficiency of cell machinery, cells can use the same protein (often called a hub protein) to participate in different cell functions by simply changing its target molecules. There are…Read article
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse…Read article
A New Filtering Technique for Removing Anti-Stokes Emission Background in Gated CW-STED Microscopy
Stimulated emission depletion (STED) microscopy is a prominent approach of super-resolution optical microscopy, which allows cellular imaging with so far unprecedented unlimited spatial resolution.…Read article