GFP
View on Science LabThe Fundamentals and History of Fluorescence and Quantum Dots
At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and…Read articleVideo Talk by Roger Tsien: Fluorescent Proteins
Live cell imaging has been revolutionized by the discovery of the green fluorescent protein (GFP). This lecture covers the history of GFP, how it folds and becomes fluorescent, how it has been mutated…Read articleFluorescent Proteins Illuminate Cell Biology
Green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria and GFP-like fluorescent proteins from other animals have had an important role in the technical innovations that have…Read articleA Brief History of Light Microscopy – From the Medieval Reading Stone to Super-Resolution
The history of microscopy begins in the Middle Ages. As far back as the 11th century, plano-convex lenses made of polished beryl were used in the Arab world as reading stones to magnify manuscripts.…Read articleFluorescent Proteins – Introduction and Photo Spectral Characteristics
The prospects of fluorescence microscopy changed dramatically with the discovery of fluorescent proteins in the 1950s. The starting point was the detection of the jellyfish Aequorea victoria green…Read articleFluorescent Proteins - From the Beginnings to the Nobel Prize
Fluorescent proteins are the fundament of recent fluorescence microscopy and its modern applications. Their discovery and consequent development was one of the most exciting innovations for life…Read articleModern Fluorescent Proteins and their Biological Applications
Here we present two review articles on fluorescent proteins and their biological applications. These first article reviews our current knowledge of blue, green, and red chromophore formation in…Read articleObese and Slim Yeast Cells
Lipids are on everyone’s lips nowadays, whether ω-3/6 fatty acids, good and bad cholesterol or just plain fat that has the annoying habit of accumulating on our hips. Serious diseases such as obesity,…Read articleCLEM: Combining the Strengths of Light and Electron Microscopy
In recent years light microscopy studies have been dominated by live cell imaging while electron microscopy has been used for high-resolution studies. Latterly, there has been increasing interest in…Read article4D Photoactivation of pa-GFP in Living Cells Using Two-Photon Excitation Laser Scanning Microscopy
We report about two-photon activation of a photoactivatable derivative of the Aequorea Victoria green fluorescent protein (pa-GFP). This special form of the molecule increases its fluorescence…Read article